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P30361 (UCRIA_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Cytochrome b6-f complex iron-sulfur subunit 1, chloroplastic
EC=1.10.9.1
Alternative name(s):
Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein 1
Rieske iron-sulfur protein 1
Short name=ISP 1
Short name=RISP 1
Gene names
Name:petC1
Synonyms:petC
OrganismNicotiana tabacum (Common tobacco)
Taxonomic identifier4097 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length228 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions By similarity.

Catalytic activity

Plastoquinol + 2 oxidized plastocyanin + 2 H+(Side 1) = plastoquinone + 2 reduced plastocyanin + 2 H+(Side 2).

Cofactor

Binds 1 2Fe-2S cluster per subunit By similarity.

Subunit structure

The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and the Rieske protein, while the 4 small subunits are petG, petL, petM and petN. The complex functions as a dimer By similarity.

Subcellular location

Plastid › chloroplast thylakoid membrane; Single-pass membrane protein By similarity. Note: The transmembrane helix obliquely spans the membrane in one monomer, and its extrinsic C-terminal domain is part of the other monomer By similarity.

Miscellaneous

This protein is 1 of 2 subunits of the cytochrome b6-f complex that are encoded in the nucleus.

The Rieske iron-sulfur protein is a high potential 2Fe-2S protein.

Sequence similarities

Contains 1 Rieske domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4949Chloroplast By similarity
Chain50 – 228179Cytochrome b6-f complex iron-sulfur subunit 1, chloroplastic
PRO_0000030694

Regions

Transmembrane72 – 9221Helical; Potential
Domain115 – 21197Rieske

Sites

Metal binding1571Iron-sulfur (2Fe-2S) By similarity
Metal binding1591Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Metal binding1751Iron-sulfur (2Fe-2S) By similarity
Metal binding1781Iron-sulfur (2Fe-2S); via pros nitrogen By similarity

Amino acid modifications

Disulfide bond162 ↔ 177 By similarity

Experimental info

Sequence conflict861P → S in CAA45705. Ref.2
Sequence conflict1481L → V in CAA45705. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P30361 [UniParc].

Last modified July 1, 1993. Version 2.
Checksum: 946B0DEA6EB1C269

FASTA22824,152
        10         20         30         40         50         60 
MASSTLSPVT QLCSSKSGLS SVSQCLLVKP MKINSHGLGK DKRMKVKCMA TSIPADDRVP 

        70         80         90        100        110        120 
DMEKRNLMNL LLLGALSLPT AGMLVPYGTF FVPPGSGGGS GGTPAKDALG NDVIASEWLK 

       130        140        150        160        170        180 
THPPGNRTLT QGLKGDPTYL VVENDGTLAT YGINAVCTHL GCVVPFNAAE NKFICPCHGS 

       190        200        210        220 
QYNNQGRVVR GPAPLSLALA HADIDDGKVV FVPWVETDFR TGEDPWWA

« Hide

References

[1]"Import and processing of the precursor of the Rieske FeS protein of tobacco chloroplasts."
Madueo F., Napier J.A., Cejudo F.J., Gray J.C.
Plant Mol. Biol. 20:289-299(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Leaf.
[2]"Different blue-light requirement for the accumulation of transcripts from nuclear genes for thylakoid proteins in Nicotiana tabacum and Lycopersicon esculentum."
Palomares R., Herrmann R.G., Oelmueller R.
J. Photochem. Photobiol. B 11:151-162(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Samsun NN.
Tissue: Seedling.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X66009 mRNA. Translation: CAA46808.1.
X64353 mRNA. Translation: CAA45705.1.
PIRS25312.

3D structure databases

ProteinModelPortalP30361.
SMRP30361. Positions 59-228.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.102.10.10. 1 hit.
InterProIPR014909. Cyt_b6-f_cplx_Fe-S_su.
IPR023960. Cyt_b6_f_Rieske.
IPR017941. Rieske_2Fe-2S.
IPR014349. Rieske_Fe-S_prot.
IPR005805. Rieske_Fe-S_prot_C.
[Graphical view]
PANTHERPTHR10134. PTHR10134. 1 hit.
PfamPF08802. CytB6-F_Fe-S. 1 hit.
PF00355. Rieske. 1 hit.
[Graphical view]
PRINTSPR00162. RIESKE.
SUPFAMSSF50022. Rieske_dom. 1 hit.
PROSITEPS51296. RIESKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUCRIA_TOBAC
AccessionPrimary (citable) accession number: P30361
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: July 1, 1993
Last modified: April 3, 2013
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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