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Protein

Arachidonate 5-lipoxygenase-activating protein

Gene

Alox5ap

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Leukotriene biosynthesis

Enzyme and pathway databases

ReactomeiR-MMU-2142688. Synthesis of 5-eicosatetraenoic acids.
R-MMU-2142691. Synthesis of Leukotrienes (LT) and Eoxins (EX).
R-MMU-2142700. Synthesis of Lipoxins (LX).

Names & Taxonomyi

Protein namesi
Recommended name:
Arachidonate 5-lipoxygenase-activating protein
Alternative name(s):
FLAP
MK-886-binding protein
Gene namesi
Name:Alox5ap
Synonyms:Flap
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:107505. Alox5ap.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8LumenalBy similarity8
Transmembranei9 – 30HelicalBy similarityAdd BLAST22
Topological domaini31 – 52CytoplasmicBy similarityAdd BLAST22
Transmembranei53 – 77HelicalBy similarityAdd BLAST25
Topological domaini78 – 80LumenalBy similarity3
Transmembranei81 – 102HelicalBy similarityAdd BLAST22
Topological domaini103 – 107CytoplasmicBy similarity5
Intramembranei108 – 115By similarity8
Transmembranei116 – 128HelicalBy similarityAdd BLAST13
Topological domaini129 – 161LumenalBy similarityAdd BLAST33

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3414408.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002177531 – 161Arachidonate 5-lipoxygenase-activating proteinAdd BLAST161

Proteomic databases

MaxQBiP30355.
PaxDbiP30355.
PeptideAtlasiP30355.
PRIDEiP30355.

PTM databases

PhosphoSitePlusiP30355.

Expressioni

Gene expression databases

BgeeiENSMUSG00000060063.
CleanExiMM_ALOX5AP.
ExpressionAtlasiP30355. baseline and differential.
GenevisibleiP30355. MM.

Interactioni

Subunit structurei

Homotrimer. Interacts with LTC4S and ALOX5.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198077. 1 interactor.
STRINGi10090.ENSMUSP00000071130.

Chemistry databases

BindingDBiP30355.

Structurei

3D structure databases

ProteinModelPortaliP30355.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni20 – 27Inhibitor bindingBy similarity8
Regioni112 – 123Inhibitor bindingBy similarityAdd BLAST12

Domaini

The C-terminal part after residue 140 is mostly disordered.By similarity

Sequence similaritiesi

Belongs to the MAPEG family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IHE0. Eukaryota.
ENOG4111T4I. LUCA.
GeneTreeiENSGT00430000030964.
HOGENOMiHOG000116372.
HOVERGENiHBG107295.
InParanoidiP30355.
KOiK20735.
OMAiNCMDAYP.
OrthoDBiEOG091G12NW.
PhylomeDBiP30355.
TreeFamiTF105328.

Family and domain databases

Gene3Di1.20.120.550. 1 hit.
InterProiIPR001446. 5_LipOase_AP.
IPR018295. FLAP/GST2/LTC4S_CS.
IPR023352. MAPEG-like_dom.
IPR001129. Membr-assoc_MAPEG.
[Graphical view]
PfamiPF01124. MAPEG. 1 hit.
[Graphical view]
PRINTSiPR00488. 5LPOXGNASEAP.
PROSITEiPS01297. FLAP_GST2_LTC4S. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30355-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDQEAVGNVV LLALVTLISV VQNAFFAHKV EHESKAHNGR SFQRTGTLAF
60 70 80 90 100
ERVYTANQNC VDAYPTFLVV LWTAGLLCSQ VPAAFAGLMY LFVRQKYFVG
110 120 130 140 150
YLGERTQSTP GYIFGKRIIL FLFLMSFAGI LNHYLIFFFG SDFENYIRTV
160
STTISPLLLI P
Length:161
Mass (Da):18,136
Last modified:October 11, 2005 - v2
Checksum:i28DA042AA18D17C8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti142D → Y in AAA37632 (PubMed:1480129).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004002 mRNA. Translation: BAB23117.1.
BC026209 mRNA. Translation: AAH26209.1.
M96554 mRNA. Translation: AAA37632.1.
CCDSiCCDS39407.1.
RefSeqiNP_033793.1. NM_009663.2.
UniGeneiMm.19844.

Genome annotation databases

EnsembliENSMUST00000071130; ENSMUSP00000071130; ENSMUSG00000060063.
GeneIDi11690.
KEGGimmu:11690.
UCSCiuc009app.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004002 mRNA. Translation: BAB23117.1.
BC026209 mRNA. Translation: AAH26209.1.
M96554 mRNA. Translation: AAA37632.1.
CCDSiCCDS39407.1.
RefSeqiNP_033793.1. NM_009663.2.
UniGeneiMm.19844.

3D structure databases

ProteinModelPortaliP30355.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198077. 1 interactor.
STRINGi10090.ENSMUSP00000071130.

Chemistry databases

BindingDBiP30355.
ChEMBLiCHEMBL3414408.

PTM databases

PhosphoSitePlusiP30355.

Proteomic databases

MaxQBiP30355.
PaxDbiP30355.
PeptideAtlasiP30355.
PRIDEiP30355.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000071130; ENSMUSP00000071130; ENSMUSG00000060063.
GeneIDi11690.
KEGGimmu:11690.
UCSCiuc009app.1. mouse.

Organism-specific databases

CTDi241.
MGIiMGI:107505. Alox5ap.

Phylogenomic databases

eggNOGiENOG410IHE0. Eukaryota.
ENOG4111T4I. LUCA.
GeneTreeiENSGT00430000030964.
HOGENOMiHOG000116372.
HOVERGENiHBG107295.
InParanoidiP30355.
KOiK20735.
OMAiNCMDAYP.
OrthoDBiEOG091G12NW.
PhylomeDBiP30355.
TreeFamiTF105328.

Enzyme and pathway databases

ReactomeiR-MMU-2142688. Synthesis of 5-eicosatetraenoic acids.
R-MMU-2142691. Synthesis of Leukotrienes (LT) and Eoxins (EX).
R-MMU-2142700. Synthesis of Lipoxins (LX).

Miscellaneous databases

PROiP30355.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000060063.
CleanExiMM_ALOX5AP.
ExpressionAtlasiP30355. baseline and differential.
GenevisibleiP30355. MM.

Family and domain databases

Gene3Di1.20.120.550. 1 hit.
InterProiIPR001446. 5_LipOase_AP.
IPR018295. FLAP/GST2/LTC4S_CS.
IPR023352. MAPEG-like_dom.
IPR001129. Membr-assoc_MAPEG.
[Graphical view]
PfamiPF01124. MAPEG. 1 hit.
[Graphical view]
PRINTSiPR00488. 5LPOXGNASEAP.
PROSITEiPS01297. FLAP_GST2_LTC4S. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAL5AP_MOUSE
AccessioniPrimary (citable) accession number: P30355
Secondary accession number(s): Q9D138
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: October 11, 2005
Last modified: November 2, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.