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P30355 (AL5AP_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Arachidonate 5-lipoxygenase-activating protein
Alternative name(s):
FLAP
MK-886-binding protein
Gene names
Name:Alox5ap
Synonyms:Flap
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length161 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes By similarity. unstructured By similarity. Ref.4

Subunit structure

Homotrimer. Interacts with LTC4S and ALOX5. Ref.4

Subcellular location

Nucleus membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity Ref.4.

Domain

The C-terminal part after residue 140 is mostly disordered By similarity.

Sequence similarities

Belongs to the MAPEG family.

Ontologies

Keywords
   Biological processLeukotriene biosynthesis
   Cellular componentEndoplasmic reticulum
Membrane
Nucleus
   DomainTransmembrane
Transmembrane helix
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to calcium ion

Inferred from electronic annotation. Source: Ensembl

leukotriene biosynthetic process

Inferred from direct assay Ref.4. Source: MGI

leukotriene production involved in inflammatory response

Inferred from mutant phenotype PubMed 10779800PubMed 9091580. Source: MGI

positive regulation of acute inflammatory response

Inferred from electronic annotation. Source: Ensembl

protein homotrimerization

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcytosol

Inferred from electronic annotation. Source: Ensembl

endoplasmic reticulum

Inferred from Biological aspect of Ancestor. Source: RefGenome

endoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

nuclear envelope

Inferred from sequence or structural similarity. Source: UniProtKB

nuclear membrane

Inferred from sequence or structural similarity. Source: UniProtKB

nucleus

Inferred from direct assay Ref.4. Source: MGI

   Molecular_functionarachidonic acid binding

Inferred from sequence or structural similarity. Source: UniProtKB

enzyme activator activity

Inferred from electronic annotation. Source: InterPro

protein binding

Inferred from physical interaction Ref.4. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 161161Arachidonate 5-lipoxygenase-activating protein
PRO_0000217753

Regions

Topological domain1 – 88Lumenal By similarity
Transmembrane9 – 3022Helical; By similarity
Topological domain31 – 5222Cytoplasmic By similarity
Transmembrane53 – 7725Helical; By similarity
Topological domain78 – 803Lumenal By similarity
Transmembrane81 – 10222Helical; By similarity
Topological domain103 – 1075Cytoplasmic By similarity
Intramembrane108 – 1158 By similarity
Transmembrane116 – 12813Helical; By similarity
Topological domain129 – 16133Lumenal By similarity
Region20 – 278Inhibitor binding By similarity
Region112 – 12312Inhibitor binding By similarity

Experimental info

Sequence conflict1421D → Y in AAA37632. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P30355 [UniParc].

Last modified October 11, 2005. Version 2.
Checksum: 28DA042AA18D17C8

FASTA16118,136
        10         20         30         40         50         60 
MDQEAVGNVV LLALVTLISV VQNAFFAHKV EHESKAHNGR SFQRTGTLAF ERVYTANQNC 

        70         80         90        100        110        120 
VDAYPTFLVV LWTAGLLCSQ VPAAFAGLMY LFVRQKYFVG YLGERTQSTP GYIFGKRIIL 

       130        140        150        160 
FLFLMSFAGI LNHYLIFFFG SDFENYIRTV STTISPLLLI P 

« Hide

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Embryo.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Mammary gland.
[3]"Cross-species comparison of 5-lipoxygenase-activating protein."
Vickers P.J., O'Neill G.P., Mancini J.A., Charleson S., Abramovitz M.
Mol. Pharmacol. 42:1014-1019(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-153.
[4]"The nuclear membrane organization of leukotriene synthesis."
Mandal A.K., Jones P.B., Bair A.M., Christmas P., Miller D., Yamin T.-T., Wisniewski D., Menke J., Evans J.F., Hyman B.T., Bacskai B., Chen M., Lee D.M., Nikolic B., Soberman R.J.
Proc. Natl. Acad. Sci. U.S.A. 105:20434-20439(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH ALOX5.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK004002 mRNA. Translation: BAB23117.1.
BC026209 mRNA. Translation: AAH26209.1.
M96554 mRNA. Translation: AAA37632.1.
CCDSCCDS39407.1.
RefSeqNP_033793.1. NM_009663.1.
UniGeneMm.19844.

3D structure databases

ProteinModelPortalP30355.
SMRP30355. Positions 1-147.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid198077. 1 interaction.
STRING10090.ENSMUSP00000071130.

PTM databases

PhosphoSiteP30355.

Proteomic databases

PaxDbP30355.
PRIDEP30355.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000071130; ENSMUSP00000071130; ENSMUSG00000060063.
GeneID11690.
KEGGmmu:11690.
UCSCuc009app.1. mouse.

Organism-specific databases

CTD241.
MGIMGI:107505. Alox5ap.

Phylogenomic databases

eggNOGNOG79474.
GeneTreeENSGT00430000030964.
HOGENOMHOG000116372.
HOVERGENHBG107295.
InParanoidP30355.
OMANCMDAYP.
PhylomeDBP30355.
TreeFamTF105328.

Gene expression databases

ArrayExpressP30355.
BgeeP30355.
CleanExMM_ALOX5AP.
GenevestigatorP30355.

Family and domain databases

Gene3D1.20.120.550. 1 hit.
InterProIPR001446. 5_LipOase_AP.
IPR018295. FLAP/GST2/LTC4S_CS.
IPR023352. MAPEG-like_dom.
IPR001129. Membr-assoc_MAPEG.
[Graphical view]
PfamPF01124. MAPEG. 1 hit.
[Graphical view]
PRINTSPR00488. 5LPOXGNASEAP.
PROSITEPS01297. FLAP_GST2_LTC4S. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio279343.
PROP30355.
SOURCESearch...

Entry information

Entry nameAL5AP_MOUSE
AccessionPrimary (citable) accession number: P30355
Secondary accession number(s): Q9D138
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: October 11, 2005
Last modified: July 9, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot