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P30355

- AL5AP_MOUSE

UniProt

P30355 - AL5AP_MOUSE

Protein

Arachidonate 5-lipoxygenase-activating protein

Gene

Alox5ap

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 2 (11 Oct 2005)
      Previous versions | rss
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    Functioni

    Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes By similarity. unstructured By similarity.By similarity

    GO - Molecular functioni

    1. arachidonic acid binding Source: UniProtKB
    2. enzyme activator activity Source: InterPro
    3. protein binding Source: MGI

    GO - Biological processi

    1. cellular response to calcium ion Source: Ensembl
    2. leukotriene biosynthetic process Source: MGI
    3. leukotriene production involved in inflammatory response Source: MGI
    4. positive regulation of acute inflammatory response Source: Ensembl
    5. protein homotrimerization Source: Ensembl

    Keywords - Biological processi

    Leukotriene biosynthesis

    Enzyme and pathway databases

    ReactomeiREACT_196544. Synthesis of Lipoxins (LX).
    REACT_206751. Synthesis of 5-eicosatetraenoic acids.
    REACT_215561. Synthesis of Leukotrienes (LT) and Eoxins (EX).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Arachidonate 5-lipoxygenase-activating protein
    Alternative name(s):
    FLAP
    MK-886-binding protein
    Gene namesi
    Name:Alox5ap
    Synonyms:Flap
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:107505. Alox5ap.

    Subcellular locationi

    Nucleus membrane 1 Publication; Multi-pass membrane protein 1 Publication. Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein By similarity

    GO - Cellular componenti

    1. cytosol Source: Ensembl
    2. endoplasmic reticulum Source: RefGenome
    3. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    4. integral component of membrane Source: UniProtKB-KW
    5. nuclear envelope Source: UniProtKB
    6. nuclear membrane Source: UniProtKB
    7. nucleus Source: MGI

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 161161Arachidonate 5-lipoxygenase-activating proteinPRO_0000217753Add
    BLAST

    Proteomic databases

    PaxDbiP30355.
    PRIDEiP30355.

    PTM databases

    PhosphoSiteiP30355.

    Expressioni

    Gene expression databases

    ArrayExpressiP30355.
    BgeeiP30355.
    CleanExiMM_ALOX5AP.
    GenevestigatoriP30355.

    Interactioni

    Subunit structurei

    Homotrimer. Interacts with LTC4S and ALOX5.1 Publication

    Protein-protein interaction databases

    BioGridi198077. 1 interaction.
    STRINGi10090.ENSMUSP00000071130.

    Structurei

    3D structure databases

    ProteinModelPortaliP30355.
    SMRiP30355. Positions 1-147.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 88LumenalBy similarity
    Topological domaini31 – 5222CytoplasmicBy similarityAdd
    BLAST
    Topological domaini78 – 803LumenalBy similarity
    Topological domaini103 – 1075CytoplasmicBy similarity
    Topological domaini129 – 16133LumenalBy similarityAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei108 – 1158By similarity

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei9 – 3022HelicalBy similarityAdd
    BLAST
    Transmembranei53 – 7725HelicalBy similarityAdd
    BLAST
    Transmembranei81 – 10222HelicalBy similarityAdd
    BLAST
    Transmembranei116 – 12813HelicalBy similarityAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni20 – 278Inhibitor bindingBy similarity
    Regioni112 – 12312Inhibitor bindingBy similarityAdd
    BLAST

    Domaini

    The C-terminal part after residue 140 is mostly disordered.By similarity

    Sequence similaritiesi

    Belongs to the MAPEG family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG79474.
    GeneTreeiENSGT00430000030964.
    HOGENOMiHOG000116372.
    HOVERGENiHBG107295.
    InParanoidiP30355.
    OMAiNCMDAYP.
    PhylomeDBiP30355.
    TreeFamiTF105328.

    Family and domain databases

    Gene3Di1.20.120.550. 1 hit.
    InterProiIPR001446. 5_LipOase_AP.
    IPR018295. FLAP/GST2/LTC4S_CS.
    IPR023352. MAPEG-like_dom.
    IPR001129. Membr-assoc_MAPEG.
    [Graphical view]
    PfamiPF01124. MAPEG. 1 hit.
    [Graphical view]
    PRINTSiPR00488. 5LPOXGNASEAP.
    PROSITEiPS01297. FLAP_GST2_LTC4S. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P30355-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDQEAVGNVV LLALVTLISV VQNAFFAHKV EHESKAHNGR SFQRTGTLAF    50
    ERVYTANQNC VDAYPTFLVV LWTAGLLCSQ VPAAFAGLMY LFVRQKYFVG 100
    YLGERTQSTP GYIFGKRIIL FLFLMSFAGI LNHYLIFFFG SDFENYIRTV 150
    STTISPLLLI P 161
    Length:161
    Mass (Da):18,136
    Last modified:October 11, 2005 - v2
    Checksum:i28DA042AA18D17C8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti142 – 1421D → Y in AAA37632. (PubMed:1480129)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK004002 mRNA. Translation: BAB23117.1.
    BC026209 mRNA. Translation: AAH26209.1.
    M96554 mRNA. Translation: AAA37632.1.
    CCDSiCCDS39407.1.
    RefSeqiNP_033793.1. NM_009663.1.
    UniGeneiMm.19844.

    Genome annotation databases

    EnsembliENSMUST00000071130; ENSMUSP00000071130; ENSMUSG00000060063.
    GeneIDi11690.
    KEGGimmu:11690.
    UCSCiuc009app.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK004002 mRNA. Translation: BAB23117.1 .
    BC026209 mRNA. Translation: AAH26209.1 .
    M96554 mRNA. Translation: AAA37632.1 .
    CCDSi CCDS39407.1.
    RefSeqi NP_033793.1. NM_009663.1.
    UniGenei Mm.19844.

    3D structure databases

    ProteinModelPortali P30355.
    SMRi P30355. Positions 1-147.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198077. 1 interaction.
    STRINGi 10090.ENSMUSP00000071130.

    PTM databases

    PhosphoSitei P30355.

    Proteomic databases

    PaxDbi P30355.
    PRIDEi P30355.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000071130 ; ENSMUSP00000071130 ; ENSMUSG00000060063 .
    GeneIDi 11690.
    KEGGi mmu:11690.
    UCSCi uc009app.1. mouse.

    Organism-specific databases

    CTDi 241.
    MGIi MGI:107505. Alox5ap.

    Phylogenomic databases

    eggNOGi NOG79474.
    GeneTreei ENSGT00430000030964.
    HOGENOMi HOG000116372.
    HOVERGENi HBG107295.
    InParanoidi P30355.
    OMAi NCMDAYP.
    PhylomeDBi P30355.
    TreeFami TF105328.

    Enzyme and pathway databases

    Reactomei REACT_196544. Synthesis of Lipoxins (LX).
    REACT_206751. Synthesis of 5-eicosatetraenoic acids.
    REACT_215561. Synthesis of Leukotrienes (LT) and Eoxins (EX).

    Miscellaneous databases

    NextBioi 279343.
    PROi P30355.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P30355.
    Bgeei P30355.
    CleanExi MM_ALOX5AP.
    Genevestigatori P30355.

    Family and domain databases

    Gene3Di 1.20.120.550. 1 hit.
    InterProi IPR001446. 5_LipOase_AP.
    IPR018295. FLAP/GST2/LTC4S_CS.
    IPR023352. MAPEG-like_dom.
    IPR001129. Membr-assoc_MAPEG.
    [Graphical view ]
    Pfami PF01124. MAPEG. 1 hit.
    [Graphical view ]
    PRINTSi PR00488. 5LPOXGNASEAP.
    PROSITEi PS01297. FLAP_GST2_LTC4S. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Embryo.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Mammary gland.
    3. "Cross-species comparison of 5-lipoxygenase-activating protein."
      Vickers P.J., O'Neill G.P., Mancini J.A., Charleson S., Abramovitz M.
      Mol. Pharmacol. 42:1014-1019(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-153.
    4. Cited for: SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH ALOX5.

    Entry informationi

    Entry nameiAL5AP_MOUSE
    AccessioniPrimary (citable) accession number: P30355
    Secondary accession number(s): Q9D138
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: October 11, 2005
    Last modified: October 1, 2014
    This is version 100 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3