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Protein

C-X-C motif chemokine 2

Gene

Cxcl2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst. Contributes to neutrophil activation during inflammation.

GO - Molecular functioni

  • chemokine activity Source: RGD

GO - Biological processi

  • cellular response to interleukin-1 Source: RGD
  • cellular response to lipopolysaccharide Source: RGD
  • immune response Source: InterPro
  • inflammatory response Source: UniProtKB-KW
  • leukocyte chemotaxis Source: RGD
  • neuron death Source: RGD
  • neutrophil chemotaxis Source: RGD
  • positive regulation of cytosolic calcium ion concentration Source: RGD
  • response to amphetamine Source: RGD
  • response to estradiol Source: RGD
  • response to gamma radiation Source: RGD
  • response to glucocorticoid Source: RGD
  • response to heat Source: RGD
  • wound healing Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Keywords - Biological processi

Chemotaxis, Inflammatory response

Enzyme and pathway databases

ReactomeiR-RNO-380108. Chemokine receptors bind chemokines.
R-RNO-418594. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
C-X-C motif chemokine 2
Alternative name(s):
Cytokine-induced neutrophil chemoattractant 3
Short name:
CINC-3
Macrophage inflammatory protein 2
Short name:
MIP2
Gene namesi
Name:Cxcl2
Synonyms:Cinc3, Mip-2, Mip2, Scyb2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi70069. Cxcl2.

Subcellular locationi

GO - Cellular componenti

  • cell Source: GOC
  • extracellular space Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 31313 PublicationsAdd
BLAST
Chaini32 – 10069C-X-C motif chemokine 2PRO_0000005062Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi36 ↔ 62By similarity
Disulfide bondi38 ↔ 78By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP30348.
PRIDEiP30348.

Expressioni

Tissue specificityi

At least expressed in the lung and trachea.

Inductioni

By lipopolysaccharide (LPS) and inflammation; in lung.

Gene expression databases

GenevisibleiP30348. RN.

Interactioni

Subunit structurei

Homotetramer.

GO - Molecular functioni

  • chemokine activity Source: RGD

Protein-protein interaction databases

BioGridi250284. 1 interaction.
STRINGi10116.ENSRNOP00000003745.

Structurei

3D structure databases

ProteinModelPortaliP30348.
SMRiP30348. Positions 29-99.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410J3WE. Eukaryota.
ENOG410ZT1C. LUCA.
GeneTreeiENSGT00530000062901.
HOGENOMiHOG000220915.
HOVERGENiHBG107789.
InParanoidiP30348.
KOiK05505.
OMAiRCITKER.
OrthoDBiEOG7NSB57.
PhylomeDBiP30348.
TreeFamiTF333433.

Family and domain databases

InterProiIPR001089. Chemokine_CXC.
IPR018048. Chemokine_CXC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PANTHERiPTHR10179. PTHR10179. 1 hit.
PfamiPF00048. IL8. 1 hit.
[Graphical view]
PRINTSiPR00437. SMALLCYTKCXC.
SMARTiSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMiSSF54117. SSF54117. 1 hit.
PROSITEiPS00471. SMALL_CYTOKINES_CXC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30348-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPPTRQLLN AVLVLLLLLA TNHQGTGVVV ASELRCQCLT TLPRVDFKNI
60 70 80 90 100
QSLTVTPPGP HCAQTEVIAT LKDGHEVCLN PEAPLVQRIV QKILNKGKAN
Length:100
Mass (Da):10,783
Last modified:April 1, 1993 - v1
Checksum:i4299DFE68FF37A9B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65647 mRNA. Translation: CAA46599.1.
S77604 mRNA. Translation: AAB33749.1.
U45965 mRNA. Translation: AAA92438.1.
AB060092 mRNA. Translation: BAB41105.1.
S45855 mRNA. No translation available.
PIRiS21467.
RefSeqiNP_446099.1. NM_053647.1.
UniGeneiRn.10230.

Genome annotation databases

EnsembliENSRNOT00000003745; ENSRNOP00000003745; ENSRNOG00000002792.
GeneIDi114105.
KEGGirno:114105.
UCSCiRGD:70069. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65647 mRNA. Translation: CAA46599.1.
S77604 mRNA. Translation: AAB33749.1.
U45965 mRNA. Translation: AAA92438.1.
AB060092 mRNA. Translation: BAB41105.1.
S45855 mRNA. No translation available.
PIRiS21467.
RefSeqiNP_446099.1. NM_053647.1.
UniGeneiRn.10230.

3D structure databases

ProteinModelPortaliP30348.
SMRiP30348. Positions 29-99.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250284. 1 interaction.
STRINGi10116.ENSRNOP00000003745.

Proteomic databases

PaxDbiP30348.
PRIDEiP30348.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000003745; ENSRNOP00000003745; ENSRNOG00000002792.
GeneIDi114105.
KEGGirno:114105.
UCSCiRGD:70069. rat.

Organism-specific databases

CTDi2920.
RGDi70069. Cxcl2.

Phylogenomic databases

eggNOGiENOG410J3WE. Eukaryota.
ENOG410ZT1C. LUCA.
GeneTreeiENSGT00530000062901.
HOGENOMiHOG000220915.
HOVERGENiHBG107789.
InParanoidiP30348.
KOiK05505.
OMAiRCITKER.
OrthoDBiEOG7NSB57.
PhylomeDBiP30348.
TreeFamiTF333433.

Enzyme and pathway databases

ReactomeiR-RNO-380108. Chemokine receptors bind chemokines.
R-RNO-418594. G alpha (i) signalling events.

Miscellaneous databases

NextBioi618277.
PROiP30348.

Gene expression databases

GenevisibleiP30348. RN.

Family and domain databases

InterProiIPR001089. Chemokine_CXC.
IPR018048. Chemokine_CXC_CS.
IPR001811. Chemokine_IL8-like_dom.
[Graphical view]
PANTHERiPTHR10179. PTHR10179. 1 hit.
PfamiPF00048. IL8. 1 hit.
[Graphical view]
PRINTSiPR00437. SMALLCYTKCXC.
SMARTiSM00199. SCY. 1 hit.
[Graphical view]
SUPFAMiSSF54117. SSF54117. 1 hit.
PROSITEiPS00471. SMALL_CYTOKINES_CXC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning, expression, and functional characterization of rat MIP-2: a neutrophil chemoattractant and epithelial cell mitogen."
    Driscoll K.E., Hassenbein D.G., Howard B.W., Isfort R.J., Cody D., Tindal M.H., Suchanek M., Carter J.M.
    J. Leukoc. Biol. 58:359-364(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Fischer 344.
    Tissue: Lung.
  2. "Modulation of neutrophil influx in glomerulonephritis in the rat with anti-macrophage inflammatory protein-2 (MIP-2) antibody."
    Feng L., Xia Y., Yoshimura T., Wilson C.B.
    J. Clin. Invest. 95:1009-1017(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Fischer.
  3. "Molecular cloning and mRNA expression of rat macrophage inflammatory protein-2."
    Farone A., Farone M., Shi M.M., Kobzik L., Paulauskis J.D.
    Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: CD Charles River.
    Tissue: Lung.
  4. "Analysis of the mechanism regulating the stability of rat macrophage inflammatory protein-2 mRNA in RBL-2H3 cells."
    Numahata K., Komagata T., Hirasawa N., Someya K., Xiao Y.Q., Ohuchi K.
    J. Cell. Biochem. 90:976-986(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
  5. "Expression of macrophage inflammatory protein-2 and KC mRNA in pulmonary inflammation."
    Huang S., Paulauskis J.D., Godleski J.J., Kobzik L.
    Am. J. Pathol. 141:981-988(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 39-91.
    Tissue: Lung.
  6. "Identification of cytokine-induced neutrophil chemoattractants (CINC), rat GRO/CINC-2 alpha and CINC-2 beta, produced by granulation tissue in culture: purification, complete amino acid sequences and characterization."
    Nakagawa H., Komorita N., Shibata F., Ikesue A., Konishi K., Fujioka M., Kato H.
    Biochem. J. 301:545-550(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 32-100.
    Strain: Wistar.
  7. "Cytokine-induced neutrophil chemoattractant (CINC)-2 alpha, a novel member of rat GRO/CINCs, is a predominant chemokine produced by lipopolysaccharide-stimulated rat macrophages in culture."
    Nakagawa H., Shiota S., Takano K., Shibata F., Kato H.
    Biochem. Biophys. Res. Commun. 220:945-948(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 32-59.
    Strain: Wistar.
  8. "Production of an interleukin-8-like chemokine by cytokine-stimulated rat NRK-49F fibroblasts and its suppression by anti-inflammatory steroids."
    Nakagawa H., Ikesue A., Hatakeyama S., Kato H., Gotoda T., Komorita N., Watanabe K., Miyai H.
    Biochem. Pharmacol. 45:1425-1430(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 32-45.

Entry informationi

Entry nameiCXCL2_RAT
AccessioniPrimary (citable) accession number: P30348
Secondary accession number(s): Q5WA60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: May 11, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.