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Protein

Mercuric reductase

Gene

merA

Organism
Streptomyces lividans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Resistance to Hg2+ in bacteria appears to be governed by a specialized system which includes mercuric reductase. MerA protein is responsible for volatilizing mercury as Hg0.

Miscellaneous

The active site is a redox-active disulfide bond.

Catalytic activityi

Hg + NADP+ + H+ = Hg2+ + NADPH.

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi471MercurySequence analysis1
Metal bindingi472MercurySequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi37 – 45FADBy similarity9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processMercuric resistance
LigandFAD, Flavoprotein, Mercury, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Mercuric reductase (EC:1.16.1.1)
Alternative name(s):
Hg(II) reductase
Gene namesi
Name:merA
OrganismiStreptomyces lividans
Taxonomic identifieri1916 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000680041 – 474Mercuric reductaseAdd BLAST474

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi45 ↔ 50Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Expressioni

Inductioni

By mercuric ions. Negatively regulated by MerR.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP30341.
SMRiP30341.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Redox-active center

Family and domain databases

Gene3Di3.30.390.30. 1 hit.
3.50.50.60. 3 hits.
InterProiView protein in InterPro
IPR023753. FAD/NAD-binding_dom.
IPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR021179. Mercury_reductase_MerA.
IPR001100. Pyr_nuc-diS_OxRdtase.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR012999. Pyr_OxRdtase_I_AS.
PfamiView protein in Pfam
PF07992. Pyr_redox_2. 1 hit.
PF02852. Pyr_redox_dim. 1 hit.
PIRSFiPIRSF000350. Mercury_reductase_MerA. 1 hit.
SUPFAMiSSF51905. SSF51905. 3 hits.
SSF55424. SSF55424. 1 hit.
TIGRFAMsiTIGR02053. MerA. 1 hit.
PROSITEiView protein in PROSITE
PS00076. PYRIDINE_REDOX_1. 1 hit.

Sequencei

Sequence statusi: Complete.

P30341-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLQAHTGYDL AIIGSGAGAF AAAIAARNKG RSVVMVERGT TGGTCVNVGC
60 70 80 90 100
VPSKALLAAA EARHGAQAAS RFPGIQATEP ALDFPALISG KDTLVGQLRA
110 120 130 140 150
EKYTDLAAEY GWQIVHGTAT FADGPMLEVA LNDGGTATVE AAHYLIATGS
160 170 180 190 200
APTAPHIDGL DQVDYLTSTT AMELQQLPEH LLILGGGYVG LEQAQLFARL
210 220 230 240 250
GSRVTLAVRS RLASREEPEI SAGIENIFRE EGITVHTRTQ LRAVRRDGEG
260 270 280 290 300
ILATLTGPDG DQQVRASHLL IATGRRSVTN GLGLERVGVK TGERGEVVVD
310 320 330 340 350
EYLRTDNPRI WAAGDVTCHP DFVYVAAAHG TLVADNALDG AERTLDYTAL
360 370 380 390 400
PKVTFTSPAI ASVGLTEAQL TEAGIAHQTR TLSLENVPRA LVNRDTRGLV
410 420 430 440 450
KLIAERGTGK LLAAHVLAEG AGDVITAATY AITAGLTVDQ LARTWHPYLT
460 470
MAEALKLAAQ TFTSDVAKLS CCAG
Length:474
Mass (Da):49,671
Last modified:April 1, 1993 - v1
Checksum:i3C85E89D0062215E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65467 Genomic DNA. Translation: CAA46460.1.
PIRiS30168.

Similar proteinsi

Entry informationi

Entry nameiMERA_STRLI
AccessioniPrimary (citable) accession number: P30341
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: September 27, 2017
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families