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Protein

N-chimaerin

Gene

Chn1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTPase-activating protein for p21-rac and a phorbol ester receptor. Involved in the assembly of neuronal locomotor circuits as a direct effector of EPHA4 in axon guidance.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri80 – 13051Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • GTPase activator activity Source: RGD
  • metal ion binding Source: UniProtKB-KW
  • phorbol ester receptor activity Source: RGD

GO - Biological processi

  • ephrin receptor signaling pathway Source: UniProtKB
  • intracellular signal transduction Source: InterPro
  • motor neuron axon guidance Source: UniProtKB
  • positive regulation of GTPase activity Source: GOC
  • regulation of axonogenesis Source: UniProtKB
  • signal transduction Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Biological processi

Neurogenesis

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
N-chimaerin
Alternative name(s):
A-chimaerin
Alpha-chimerin
N-chimerin
Short name:
NC
Rho GTPase-activating protein 2
Gene namesi
Name:Chn1
Synonyms:Arhgap2, Chn
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620139. Chn1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 334334N-chimaerinPRO_0000056696Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei215 – 2151PhosphothreonineCombined sources

Post-translational modificationi

Phosphorylated. Phosphorylation is EPHA4 kinase activity-dependent (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP30337.
PRIDEiP30337.

PTM databases

iPTMnetiP30337.
PhosphoSiteiP30337.

Expressioni

Tissue specificityi

In neurons in brain regions that are involved in learning and memory processes.

Developmental stagei

Increases in amount during brain development coincident with synaptogenesis.

Gene expression databases

GenevisibleiP30337. RN.

Interactioni

Subunit structurei

Interacts with EPHA4; effector of EPHA4 in axon guidance linking EPHA4 activation to RAC1 regulation.By similarity

Protein-protein interaction databases

DIPiDIP-39378N.
IntActiP30337. 1 interaction.
STRINGi10116.ENSRNOP00000060544.

Structurei

3D structure databases

ProteinModelPortaliP30337.
SMRiP30337. Positions 78-334.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini143 – 334192Rho-GAPPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 phorbol-ester/DAG-type zinc finger.PROSITE-ProRule annotation
Contains 1 Rho-GAP domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri80 – 13051Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IPFQ. Eukaryota.
ENOG410YM3I. LUCA.
HOVERGENiHBG080489.
InParanoidiP30337.
OMAiMWGLMAQ.
OrthoDBiEOG73JKVC.
PhylomeDBiP30337.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
InterProiIPR020454. DAG/PE-bd.
IPR002219. PE/DAG-bd.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
PRINTSiPR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
PROSITEiPS50238. RHOGAP. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30337-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSKESWSGR KTNRATVHKS KQEGRQQDLL IAALGMKLGS QKSSVTIWQP
60 70 80 90 100
LKLFAYSQLT SLVRRATLKE NEQIPKYEKV HNFKVHTFRG PHWCEYCANF
110 120 130 140 150
MWGLIAQGVK CADCGLNVHK QCSKMVPNDC KPDLKHVKKV YSCDLTTLVK
160 170 180 190 200
AHITKRPMVV DMCIREIESR GLNSEGLYRV SGFSDLIEDV KMAFDRDGEK
210 220 230 240 250
ADISVNMYED INIITGALKL YFRDLPIPLI TYDAYPKFIE SAKIVDPDEQ
260 270 280 290 300
LETLHEALRS LPPAHCETLR YLMAHLKRVT LHEKENLMSA ENLGIVFGPT
310 320 330
LMRSPELDPM AALNDIRYQR LVVELLIKNE DILF
Length:334
Mass (Da):38,224
Last modified:September 5, 2006 - v2
Checksum:iD4DB599737D171FB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti21 – 211K → N in CAA47672 (PubMed:1445199).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67250 mRNA. Translation: CAA47672.1.
BC094519 mRNA. Translation: AAH94519.1.
PIRiS29128.
RefSeqiNP_114472.1. NM_032083.1.
UniGeneiRn.11166.

Genome annotation databases

GeneIDi84030.
KEGGirno:84030.
UCSCiRGD:620139. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67250 mRNA. Translation: CAA47672.1.
BC094519 mRNA. Translation: AAH94519.1.
PIRiS29128.
RefSeqiNP_114472.1. NM_032083.1.
UniGeneiRn.11166.

3D structure databases

ProteinModelPortaliP30337.
SMRiP30337. Positions 78-334.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-39378N.
IntActiP30337. 1 interaction.
STRINGi10116.ENSRNOP00000060544.

PTM databases

iPTMnetiP30337.
PhosphoSiteiP30337.

Proteomic databases

PaxDbiP30337.
PRIDEiP30337.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi84030.
KEGGirno:84030.
UCSCiRGD:620139. rat.

Organism-specific databases

CTDi1123.
RGDi620139. Chn1.

Phylogenomic databases

eggNOGiENOG410IPFQ. Eukaryota.
ENOG410YM3I. LUCA.
HOVERGENiHBG080489.
InParanoidiP30337.
OMAiMWGLMAQ.
OrthoDBiEOG73JKVC.
PhylomeDBiP30337.

Miscellaneous databases

PROiP30337.

Gene expression databases

GenevisibleiP30337. RN.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
InterProiIPR020454. DAG/PE-bd.
IPR002219. PE/DAG-bd.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
PRINTSiPR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
PROSITEiPS50238. RHOGAP. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Developmental regulation and neuronal expression of the mRNA of rat n-chimaerin, a p21rac GAP:cDNA sequence."
    Lim H.H., Michael G.J., Smith P., Lim L., Hall C.
    Biochem. J. 287:415-422(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites."
    Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.
    Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-215, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCHIN_RAT
AccessioniPrimary (citable) accession number: P30337
Secondary accession number(s): Q505J4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: September 5, 2006
Last modified: June 8, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.