Probable cadmium-transporting ATPase - Bacillus pseudofirmus (strain OF4)

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P30336 (CADA_BACPE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 92. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Probable cadmium-transporting ATPase
EC=3.6.3.3

Alternative name(s):
Cadmium efflux ATPase

Gene names

Name:	cadA
Ordered Locus Names:	BpOF4_21834

Encoded on

Plasmid pBpOF4-02 Ref.2

Organism

Bacillus pseudofirmus (strain OF4) [Complete proteome] [HAMAP]

Taxonomic identifier

398511 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	723 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This electroneutral antiporter ejects one cadmium ion while accumulating two protons by an energy-dependent efflux mechanism.
Catalytic activity	ATP + H₂O + Cd²⁺(In) = ADP + phosphate + Cd²⁺(Out).
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily. [View classification] Contains 1 HMA domain.

Ontologies

Keywords
Biological process	Antiport Cadmium resistance Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Cadmium Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro response to cadmium ion Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW antiporter activity Inferred from electronic annotation. Source: UniProtKB-KW cadmium-exporting ATPase activity Inferred from electronic annotation. Source: EC cation channel activity Inferred from electronic annotation. Source: InterPro copper-exporting ATPase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 723

723

Probable cadmium-transporting ATPase

PRO_0000046245

Regions

Transmembrane

103 – 123

Helical; Potential

Transmembrane

127 – 147

Helical; Potential

Transmembrane

168 – 188

Helical; Potential

Transmembrane

329 – 349

Helical; Potential

Transmembrane

361 – 381

Helical; Potential

Transmembrane

671 – 690

Helical; Potential

Transmembrane

694 – 716

Helical; Potential

Domain

13 – 76

HMA

Sites

Active site

412

4-aspartylphosphate intermediate Probable

Metal binding

Cadmium Potential

Metal binding

Cadmium Potential

Experimental info

Sequence conflict

113

A → T in AAA22858. Ref.1

Sequence conflict

261

A → T in AAA22858. Ref.1

Sequence conflict

339

A → T in AAA22858. Ref.1

Sequence conflict

660

F → S in AAA22858. Ref.1

Sequence conflict

687

L → S in AAA22858. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P30336 [UniParc].

Last modified June 15, 2010. Version 2.
Checksum: 975D709B3754D5EF
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FASTA

723

78,204

        10         20         30         40         50         60 
MSDQKAITSE QEMKAYRVQG FTCANCAGKF EKNVKQLSGV EDAKVNFGAS KIAVYGNATI 

        70         80         90        100        110        120 
EELEKAGAFE NLKVTPEKSA RQASQEVKED TKEDKVPFYK KHSTLLYASL LIAFGYLSSY 

       130        140        150        160        170        180 
VNGEENIVTT LLFLASMFIG GLSLFKVGLQ NLLRFEFDMK TLMTVAVIGG AIIGEWAEVA 

       190        200        210        220        230        240 
IVVILFAISE ALERFSMDRA RQSIRSLMDI APKEALVKRN GQEIMIHVDD IAVGDIMIVK 

       250        260        270        280        290        300 
PGQKIAMDGV VVSGYSAVNQ AAITGESVPV EKTVDNEVFA GTLNEEGLLE VEITKLVEDT 

       310        320        330        340        350        360 
TISKIIHLVE EAQGERAPSQ AFVDKFAKYY TPIIMIIAAL VAIVPPLFFD GSWETWIYQG 

       370        380        390        400        410        420 
LAVLVVGCPC ALVISTPISI VSAIGNAAKK GVLVKGGVYL EEMGALKAIA FDKTGTLTKG 

       430        440        450        460        470        480 
VPAVTDYNVL NKQINEKELL SIITALEYRS QHPLASAIMK KAEEENITYS DVQVEDFSSI 

       490        500        510        520        530        540 
TGKGIKGIVN GTTYYIGSPK LFKELLTNDF DKDLEQNVTT LQNQGKTAMI IGTEKEILAV 

       550        560        570        580        590        600 
IAVADEVRES SKEILQKLHQ LGIKKTIMLT GDNKGTANAI GGQVGVSDIE AELMPQDKLD 

       610        620        630        640        650        660 
FIKQLRSEYG NVAMVGDGVN DAPALAASTV GIAMGGAGTD TALETADVAL MGDDLRKLPF 

       670        680        690        700        710        720 
TVKLSRKTLN IIKANITFAI AIKFIALLLV IPGWLTLWIA ILSDMGATLL VALNGLRLMR 


VKE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The cadC gene product of alkaliphilic Bacillus firmus OF4 partially restores Na+ resistance to an Escherichia coli strain lacking an Na+/H+ antiporter (NhaA)." Ivey D.M., Guffanti A.A., Shen Z., Kudyan N., Krulwich T.A. J. Bacteriol. 174:4878-4884(1992) [PubMed: 1321115] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of Bacillus pseudofirmus OF4." Hu F.Z. Submitted (JAN-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OF4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M90750 Genomic DNA. Translation: AAA22858.1. CP001880 Genomic DNA. Translation: ADC52362.1.
PIR	D42707.
RefSeq	YP_003429254.1. NC_013793.1.
3D structure databases
ProteinModelPortal	P30336.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000289258; EBBACP00000286704; EBBACG00000288766.
GeneID	8769369.
GenomeReviews	Gene locus BpOF4_21834 in contig CP001880_GR.
KEGG	bpf:BpOF4_21834.
PATRIC	31951034. VBIBacPse80461_4179.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00070000031791.
ProtClustDB	CLSK793380.
Family and domain databases
InterPro	IPR008250. ATPase_P-typ_ATPase-assoc-dom. IPR006403. ATPase_P-typ_cat/Cu-transptr. IPR001366. ATPase_P-typ_Cd-transp. IPR006404. ATPase_P-typ_Cd/Co/Hg/Pb/Zn. IPR023300. ATPase_P-typ_cyto_domA. IPR023299. ATPase_P-typ_cyto_domN. IPR006416. ATPase_P-typ_heavy-metal. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_P_site. IPR005834. Dehalogen-like_hydro. IPR023214. HAD-like_dom. IPR017969. Heavy-metal-associated_CS. IPR006121. HeavyMe-assoc_HMA. [Graphical view]
Gene3D	G3DSA:2.70.150.10. ATPase_P-typ_cyto_domA. 1 hit. G3DSA:3.40.1110.10. ATPase_P-typ_cyto_domN. 1 hit. G3DSA:3.40.50.1000. HAD-like_dom. 2 hits.
KO	K01534.
Pfam	PF00122. E1-E2_ATPase. 1 hit. PF00403. HMA. 1 hit. PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE. PR00941. CDATPASE.
SUPFAM	SSF56784. HAD-like_dom. 1 hit. SSF55008. HeavyMe_transpt. 1 hit.
TIGRFAMs	TIGR01511. ATPase-IB1_Cu. 1 hit. TIGR01512. ATPase-IB2_Cd. 1 hit. TIGR01525. ATPase-IB_hvy. 1 hit. TIGR01494. ATPase_P-type. 1 hit.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. PS01047. HMA_1. 1 hit. PS50846. HMA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CADA_BACPE

Accession

Primary (citable) accession number: P30336
Secondary accession number(s): D3G1Y6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	June 15, 2010
Last modified:	January 25, 2012
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents