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Protein

Probable cadmium-transporting ATPase

Gene

cadA

Organism
Bacillus pseudofirmus (strain OF4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This electroneutral antiporter ejects one cadmium ion while accumulating two protons by an energy-dependent efflux mechanism.

Catalytic activityi

ATP + H2O + Cd2+(In) = ADP + phosphate + Cd2+(Out).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi23 – 231CadmiumPROSITE-ProRule annotation
Metal bindingi26 – 261CadmiumPROSITE-ProRule annotation
Active sitei412 – 41214-aspartylphosphate intermediateCurated

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antiport, Cadmium resistance, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Cadmium, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBPSE398511:GJI9-4392-MONOMER.
RETL1328306-WGS:GSTH-3794-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cadmium-transporting ATPase (EC:3.6.3.3)
Alternative name(s):
Cadmium efflux ATPase
Gene namesi
Name:cadA
Ordered Locus Names:BpOF4_21834
Encoded oniPlasmid pBpOF4-021 Publication
OrganismiBacillus pseudofirmus (strain OF4)
Taxonomic identifieri398511 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001544 Componenti: Plasmid pBpOF4-02

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei103 – 12321HelicalSequence analysisAdd
BLAST
Transmembranei127 – 14721HelicalSequence analysisAdd
BLAST
Transmembranei168 – 18821HelicalSequence analysisAdd
BLAST
Transmembranei329 – 34921HelicalSequence analysisAdd
BLAST
Transmembranei361 – 38121HelicalSequence analysisAdd
BLAST
Transmembranei671 – 69020HelicalSequence analysisAdd
BLAST
Transmembranei694 – 71623HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 723723Probable cadmium-transporting ATPasePRO_0000046245Add
BLAST

Keywords - PTMi

Phosphoprotein

Structurei

3D structure databases

ProteinModelPortaliP30336.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 7664HMAPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HMA domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000250399.
KOiK01534.

Family and domain databases

Gene3Di2.70.150.10. 1 hit.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR017969. Heavy-metal-associated_CS.
IPR006121. HMA_dom.
IPR027256. P-typ_ATPase_IB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF00403. HMA. 1 hit.
[Graphical view]
PRINTSiPR00941. CDATPASE.
SUPFAMiSSF55008. SSF55008. 1 hit.
SSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01525. ATPase-IB_hvy. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
PS01047. HMA_1. 1 hit.
PS50846. HMA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30336-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDQKAITSE QEMKAYRVQG FTCANCAGKF EKNVKQLSGV EDAKVNFGAS
60 70 80 90 100
KIAVYGNATI EELEKAGAFE NLKVTPEKSA RQASQEVKED TKEDKVPFYK
110 120 130 140 150
KHSTLLYASL LIAFGYLSSY VNGEENIVTT LLFLASMFIG GLSLFKVGLQ
160 170 180 190 200
NLLRFEFDMK TLMTVAVIGG AIIGEWAEVA IVVILFAISE ALERFSMDRA
210 220 230 240 250
RQSIRSLMDI APKEALVKRN GQEIMIHVDD IAVGDIMIVK PGQKIAMDGV
260 270 280 290 300
VVSGYSAVNQ AAITGESVPV EKTVDNEVFA GTLNEEGLLE VEITKLVEDT
310 320 330 340 350
TISKIIHLVE EAQGERAPSQ AFVDKFAKYY TPIIMIIAAL VAIVPPLFFD
360 370 380 390 400
GSWETWIYQG LAVLVVGCPC ALVISTPISI VSAIGNAAKK GVLVKGGVYL
410 420 430 440 450
EEMGALKAIA FDKTGTLTKG VPAVTDYNVL NKQINEKELL SIITALEYRS
460 470 480 490 500
QHPLASAIMK KAEEENITYS DVQVEDFSSI TGKGIKGIVN GTTYYIGSPK
510 520 530 540 550
LFKELLTNDF DKDLEQNVTT LQNQGKTAMI IGTEKEILAV IAVADEVRES
560 570 580 590 600
SKEILQKLHQ LGIKKTIMLT GDNKGTANAI GGQVGVSDIE AELMPQDKLD
610 620 630 640 650
FIKQLRSEYG NVAMVGDGVN DAPALAASTV GIAMGGAGTD TALETADVAL
660 670 680 690 700
MGDDLRKLPF TVKLSRKTLN IIKANITFAI AIKFIALLLV IPGWLTLWIA
710 720
ILSDMGATLL VALNGLRLMR VKE
Length:723
Mass (Da):78,204
Last modified:June 15, 2010 - v2
Checksum:i975D709B3754D5EF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti113 – 1131A → T in AAA22858 (PubMed:1321115).Curated
Sequence conflicti261 – 2611A → T in AAA22858 (PubMed:1321115).Curated
Sequence conflicti339 – 3391A → T in AAA22858 (PubMed:1321115).Curated
Sequence conflicti660 – 6601F → S in AAA22858 (PubMed:1321115).Curated
Sequence conflicti687 – 6871L → S in AAA22858 (PubMed:1321115).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90750 Genomic DNA. Translation: AAA22858.1.
CP001880 Genomic DNA. Translation: ADC52362.1.
PIRiD42707.
RefSeqiWP_012961265.1. NC_013793.1.

Genome annotation databases

EnsemblBacteriaiADC52362; ADC52362; BpOF4_21834.
KEGGibpf:BpOF4_21834.
PATRICi31951034. VBIBacPse80461_4179.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90750 Genomic DNA. Translation: AAA22858.1.
CP001880 Genomic DNA. Translation: ADC52362.1.
PIRiD42707.
RefSeqiWP_012961265.1. NC_013793.1.

3D structure databases

ProteinModelPortaliP30336.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADC52362; ADC52362; BpOF4_21834.
KEGGibpf:BpOF4_21834.
PATRICi31951034. VBIBacPse80461_4179.

Phylogenomic databases

HOGENOMiHOG000250399.
KOiK01534.

Enzyme and pathway databases

BioCyciBPSE398511:GJI9-4392-MONOMER.
RETL1328306-WGS:GSTH-3794-MONOMER.

Family and domain databases

Gene3Di2.70.150.10. 1 hit.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR017969. Heavy-metal-associated_CS.
IPR006121. HMA_dom.
IPR027256. P-typ_ATPase_IB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF00403. HMA. 1 hit.
[Graphical view]
PRINTSiPR00941. CDATPASE.
SUPFAMiSSF55008. SSF55008. 1 hit.
SSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01525. ATPase-IB_hvy. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
PS01047. HMA_1. 1 hit.
PS50846. HMA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The cadC gene product of alkaliphilic Bacillus firmus OF4 partially restores Na+ resistance to an Escherichia coli strain lacking an Na+/H+ antiporter (NhaA)."
    Ivey D.M., Guffanti A.A., Shen Z., Kudyan N., Krulwich T.A.
    J. Bacteriol. 174:4878-4884(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Genome of alkaliphilic Bacillus pseudofirmus OF4 reveals adaptations that support the ability to grow in an external pH range from 7.5 to 11.4."
    Janto B., Ahmed A., Ito M., Liu J., Hicks D.B., Pagni S., Fackelmayer O.J., Smith T.A., Earl J., Elbourne L.D., Hassan K., Paulsen I.T., Kolsto A.B., Tourasse N.J., Ehrlich G.D., Boissy R., Ivey D.M., Li G.
    , Xue Y., Ma Y., Hu F.Z., Krulwich T.A.
    Environ. Microbiol. 13:3289-3309(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: OF4.
    Plasmid: pBpOF4-02

Entry informationi

Entry nameiCADA_BACPE
AccessioniPrimary (citable) accession number: P30336
Secondary accession number(s): D3G1Y6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: June 15, 2010
Last modified: April 13, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.