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Protein

RNA polymerase sigma-54 factor 2

Gene

rpoN2

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi403 – 422H-T-H motifSequence analysisAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Nucleotidyltransferase, Sigma factor, Transferase
Biological processNitrogen fixation, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase sigma-54 factor 2
Gene namesi
Name:rpoN2
Ordered Locus Names:blr0723
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
Proteomesi
  • UP000002526 Componenti: Chromosome

Subcellular locationi

Keywords - Cellular componenti

DNA-directed RNA polymerase

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002055251 – 537RNA polymerase sigma-54 factor 2Add BLAST537

Expressioni

Inductioni

Negatively autoregulated.

Interactioni

Protein-protein interaction databases

STRINGi224911.blr0723.

Structurei

3D structure databases

ProteinModelPortaliP30333.
SMRiP30333.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi492 – 500RPON box9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi11 – 29Gln-richAdd BLAST19

Sequence similaritiesi

Belongs to the sigma-54 factor family.Curated

Phylogenomic databases

eggNOGiENOG4105DTW. Bacteria.
COG1508. LUCA.
HOGENOMiHOG000071904.
InParanoidiP30333.
KOiK03092.
OMAiVTTQKFM.
OrthoDBiPOG091H02EW.
PhylomeDBiP30333.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiView protein in InterPro
IPR000394. RNA_pol_sigma_54.
IPR007046. RNA_pol_sigma_54_core-bd.
IPR007634. RNA_pol_sigma_54_DNA-bd.
IPR036388. WH-like_DNA-bd_sf.
PANTHERiPTHR32248. PTHR32248. 1 hit.
PfamiView protein in Pfam
PF00309. Sigma54_AID. 1 hit.
PF04963. Sigma54_CBD. 1 hit.
PF04552. Sigma54_DBD. 1 hit.
PIRSFiPIRSF000774. RpoN. 1 hit.
PRINTSiPR00045. SIGMA54FCT.
TIGRFAMsiTIGR02395. rpoN_sigma. 1 hit.
PROSITEiView protein in PROSITE
PS00717. SIGMA54_1. 1 hit.
PS00718. SIGMA54_2. 1 hit.
PS50044. SIGMA54_3. 1 hit.

Sequencei

Sequence statusi: Complete.

P30333-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALTQRLEFR QSQSLVMTPQ LMQAIKLLQL SNLDLTTFVE EELERNPLLE
60 70 80 90 100
RANDEASGGE APAEAGQFSD SDGGHNDEPG GGPGEAFEPG QEEWMSKDLG
110 120 130 140 150
TRAEIEQTLD TGLDNVFSEE PAEAAARNAQ DAAPTTYTEW GGGASGDEDY
160 170 180 190 200
NLEAFVAAEV TLGDHLAEQL SVAFTAPAQR MIGQYLIDLV DEAGYLPPDL
210 220 230 240 250
GQAAERLGAS QQEVEDVLAV LQKFDPPGVC ARNLSECLAI QLRELDRYDP
260 270 280 290 300
AMQALVEHLD LLAKRDIAGL RKVCGVDDED IADMIGEIRR LNPKPGMKFG
310 320 330 340 350
AARLQTMVPD VYVRPGPDGG WHVELNSDTL PRVLVNQTYY SELSKKIGKD
360 370 380 390 400
GDKSYFTDAL QNATWLVRAL DQRARTILKV ATEIVRQQDG FFTHGVAHLR
410 420 430 440 450
PLNLKAVADA IQMHESTVSR VTANKYMATN RGTFELKYFF TASIASADGG
460 470 480 490 500
EAHSAEAVRH HIKQLIDSEA PAAILSDDTI VERLRASGID IARRTVAKYR
510 520 530
EAMRIPSSVQ RRRDKQSALG NVLSTAMSDR SRNPEPA
Length:537
Mass (Da):58,832
Last modified:April 1, 1993 - v1
Checksum:i163D40BDAAE540EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59243 Genomic DNA. Translation: AAA26241.1.
BA000040 Genomic DNA. Translation: BAC45988.1.
PIRiB38179.
RefSeqiNP_767363.1. NC_004463.1.
WP_011083548.1. NZ_CP011360.1.

Genome annotation databases

EnsemblBacteriaiBAC45988; BAC45988; BAC45988.
GeneIDi1054195.
KEGGibja:blr0723.
PATRICifig|224911.44.peg.95.

Similar proteinsi

Entry informationi

Entry nameiRP55_BRADU
AccessioniPrimary (citable) accession number: P30333
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: October 25, 2017
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families