ID DPOL_THELI Reviewed; 1702 AA. AC P30317; DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot. DT 01-APR-1993, sequence version 1. DT 08-NOV-2023, entry version 145. DE RecName: Full=DNA polymerase; DE EC=2.7.7.7; DE AltName: Full=Vent DNA polymerase; DE Contains: DE RecName: Full=Endonuclease PI-TliII; DE EC=3.1.-.-; DE AltName: Full=IVPS2; DE AltName: Full=Tli pol-1 intein; DE Contains: DE RecName: Full=Endonuclease PI-TliI; DE EC=3.1.-.-; DE AltName: Full=IVPS1; DE AltName: Full=Tli pol-2 intein; GN Name=pol; OS Thermococcus litoralis. OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae; OC Thermococcus. OX NCBI_TaxID=2265; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE. RX PubMed=1608969; DOI=10.1073/pnas.89.12.5577; RA Perler F.B., Comb D.G., Jack W.E., Moran L.S., Qiang B., Kucera R.B., RA Benner J., Slatko B.E., Nwankwo D.O., Hempstead S.K., Carlow C.K.S., RA Jannasch H.; RT "Intervening sequences in an Archaea DNA polymerase gene."; RL Proc. Natl. Acad. Sci. U.S.A. 89:5577-5581(1992). RN [2] RP PROTEIN SPLICING. RX PubMed=1475179; DOI=10.1093/nar/20.23.6153; RA Hodges R.A., Perler F.B., Noren C.J., Jack W.E.; RT "Protein splicing removes intervening sequences in an archaea DNA RT polymerase."; RL Nucleic Acids Res. 20:6153-6157(1992). CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase CC exhibits 3' to 5' exonuclease activity. CC -!- FUNCTION: Intein encoded endonucleases are thought to mediate intein CC mobility by site-specific recombination initiated by endonuclease CC cleavage at the 'homing site' in gene that lack the intein. CC -!- CATALYTIC ACTIVITY: CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339, CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560, CC ChEBI:CHEBI:173112; EC=2.7.7.7; CC -!- PTM: This protein undergoes a protein self splicing that involves a CC post-translational excision of the two intervening regions (inteins) CC followed by peptide ligation. CC -!- BIOTECHNOLOGY: Used in the PCR method because of its high CC thermostability and low error rate. Sold by New England Biolabs. CC -!- SIMILARITY: Belongs to the DNA polymerase type-B family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M74198; AAA72100.1; -; Unassigned_DNA. DR EMBL; M74198; AAA72101.1; -; Unassigned_DNA. DR PIR; S42459; S42459. DR AlphaFoldDB; P30317; -. DR SMR; P30317; -. DR GeneID; 16550440; -. DR OMA; YAHNSYY; -. DR BRENDA; 2.7.7.7; 6302. DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW. DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW. DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW. DR GO; GO:0000166; F:nucleotide binding; IEA:InterPro. DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW. DR GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro. DR GO; GO:0006314; P:intron homing; IEA:UniProtKB-KW. DR CDD; cd05780; DNA_polB_Kod1_like_exo; 1. DR CDD; cd00081; Hint; 3. DR Gene3D; 1.10.132.60; DNA polymerase family B, C-terminal domain; 1. DR Gene3D; 3.30.342.10; DNA Polymerase, chain B, domain 1; 1. DR Gene3D; 2.170.16.10; Hedgehog/Intein (Hint) domain; 2. DR Gene3D; 3.10.28.10; Homing endonucleases; 2. DR Gene3D; 1.10.8.1330; Intein homing endonuclease, domain III; 1. DR Gene3D; 1.10.10.1010; Intein homing endonuclease, domain IV; 1. DR Gene3D; 3.90.1600.10; Palm domain of DNA polymerase; 3. DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1. DR InterPro; IPR006172; DNA-dir_DNA_pol_B. DR InterPro; IPR006133; DNA-dir_DNA_pol_B_exonuc. DR InterPro; IPR006134; DNA-dir_DNA_pol_B_multi_dom. DR InterPro; IPR043502; DNA/RNA_pol_sf. DR InterPro; IPR042087; DNA_pol_B_thumb. DR InterPro; IPR023211; DNA_pol_palm_dom_sf. DR InterPro; IPR003586; Hint_dom_C. DR InterPro; IPR003587; Hint_dom_N. DR InterPro; IPR036844; Hint_dom_sf. DR InterPro; IPR027434; Homing_endonucl. DR InterPro; IPR006142; INTEIN. DR InterPro; IPR030934; Intein_C. DR InterPro; IPR004042; Intein_endonuc. DR InterPro; IPR006141; Intein_N. DR InterPro; IPR004860; LAGLIDADG_2. DR InterPro; IPR041005; PI-TkoII_IV. DR InterPro; IPR012337; RNaseH-like_sf. DR InterPro; IPR036397; RNaseH_sf. DR NCBIfam; TIGR01443; intein_Cterm; 2. DR NCBIfam; TIGR01445; intein_Nterm; 2. DR NCBIfam; TIGR00592; pol2; 2. DR PANTHER; PTHR10322; DNA POLYMERASE CATALYTIC SUBUNIT; 1. DR PANTHER; PTHR10322:SF23; DNA POLYMERASE DELTA CATALYTIC SUBUNIT; 1. DR Pfam; PF00136; DNA_pol_B; 3. DR Pfam; PF03104; DNA_pol_B_exo1; 2. DR Pfam; PF14890; Intein_splicing; 2. DR Pfam; PF14528; LAGLIDADG_3; 2. DR Pfam; PF18714; PI-TkoII_IV; 1. DR PRINTS; PR00379; INTEIN. DR SMART; SM00305; HintC; 2. DR SMART; SM00306; HintN; 2. DR SMART; SM00486; POLBc; 1. DR SUPFAM; SSF56672; DNA/RNA polymerases; 2. DR SUPFAM; SSF51294; Hedgehog/intein (Hint) domain; 2. DR SUPFAM; SSF55608; Homing endonucleases; 2. DR SUPFAM; SSF53098; Ribonuclease H-like; 1. DR PROSITE; PS50818; INTEIN_C_TER; 2. DR PROSITE; PS50819; INTEIN_ENDONUCLEASE; 2. DR PROSITE; PS50817; INTEIN_N_TER; 2. PE 1: Evidence at protein level; KW Autocatalytic cleavage; Direct protein sequencing; DNA replication; KW DNA-binding; DNA-directed DNA polymerase; Endonuclease; Hydrolase; KW Intron homing; Nuclease; Nucleotidyltransferase; Protein splicing; Repeat; KW Transferase. FT CHAIN 1..494 FT /note="DNA polymerase, 1st part" FT /id="PRO_0000007338" FT CHAIN 495..1032 FT /note="Endonuclease PI-TliII" FT /id="PRO_0000007339" FT CHAIN 1033..1081 FT /note="DNA polymerase, 2nd part" FT /id="PRO_0000007340" FT CHAIN 1082..1471 FT /note="Endonuclease PI-TliI" FT /id="PRO_0000007341" FT CHAIN 1472..1702 FT /note="DNA polymerase, 3rd part" FT /id="PRO_0000007342" FT DOMAIN 776..909 FT /note="DOD-type homing endonuclease 1" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00273" FT DOMAIN 1229..1368 FT /note="DOD-type homing endonuclease 2" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00273" SQ SEQUENCE 1702 AA; 197294 MW; 21D6B98C75F53B20 CRC64; MILDTDYITK DGKPIIRIFK KENGEFKIEL DPHFQPYIYA LLKDDSAIEE IKAIKGERHG KTVRVLDAVK VRKKFLGREV EVWKLIFEHP QDVPAMRGKI REHPAVVDIY EYDIPFAKRY LIDKGLIPME GDEELKLLAF DIETFYHEGD EFGKGEIIMI SYADEEEARV ITWKNIDLPY VDVVSNEREM IKRFVQVVKE KDPDVIITYN GDNFDLPYLI KRAEKLGVRL VLGRDKEHPE PKIQRMGDSF AVEIKGRIHF DLFPVVRRTI NLPTYTLEAV YEAVLGKTKS KLGAEEIAAI WETEESMKKL AQYSMEDARA TYELGKEFFP MEAELAKLIG QSVWDVSRSS TGNLVEWYLL RVAYARNELA PNKPDEEEYK RRLRTTYLGG YVKEPEKGLW ENIIYLDFRS LYPSIIVTHN VSPDTLEKEG CKNYDVAPIV GYRFCKDFPG FIPSILGDLI AMRQDIKKKM KSTIDPIEKK MLDYRQRAIK LLANSILPNE WLPIIENGEI KFVKIGEFIN SYMEKQKENV KTVENTEVLE VNNLFAFSFN KKIKESEVKK VKALIRHKYK GKAYEIQLSS GRKINITAGH SLFTVRNGEI KEVSGDGIKE GDLIVAPKKI KLNEKGVSIN IPELISDLSE EETADIVMTI SAKGRKNFFK GMLRTLRWMF GEENRRIRTF NRYLFHLEKL GLIKLLPRGY EVTDWERLKK YKQLYEKLAG SVKYNGNKRE YLVMFNEIKD FISYFPQKEL EEWKIGTLNG FRTNCILKVD EDFGKLLGYY VSEGYAGAQK NKTGGISYSV KLYNEDPNVL ESMKNVAEKF FGKVRVDRNC VSISKKMAYL VMKCLCGALA ENKRIPSVIL TSPEPVRWSF LEAYFTGDGD IHPSKRFRLS TKSELLANQL VFLLNSLGIS SVKIGFDSGV YRVYINEDLQ FPQTSREKNT YYSNLIPKEI LRDVFGKEFQ KNMTFKKFKE LVDSGKLNRE KAKLLEFFIN GDIVLDRVKS VKEKDYEGYV YDLSVEDNEN FLVGFGLLYA HNSYYGYMGY PKARWYSKEC AESVTAWGRH YIEMTIREIE EKFGFKVLYA DSVSGESEII IRQNGKIRFV KIKDLFSKVD YSIGEKEYCI LEGVEALTLD DDGKLVWKPV PYVMRHRANK RMFRIWLTNS WYIDVTEDHS LIGYLNTSKT KTAKKIGERL KEVKPFELGK AVKSLICPNA PLKDENTKTS EIAVKFWELV GLIVGDGNWG GDSRWAEYYL GLSTGKDAEE IKQKLLEPLK TYGVISNYYP KNEKGDFNIL AKSLVKFMKR HFKDEKGRRK IPEFMYELPV TYIEAFLRGL FSADGTVTIR KGVPEIRLTN IDADFLREVR KLLWIVGISN SIFAETTPNR YNGVSTGTYS KHLRIKNKWR FAERIGFLIE RKQKRLLEHL KSARVKRNTI DFGFDLVHVK KVEEIPYEGY VYDIEVEETH RFFANNILVH NTDGFYATIP GEKPELIKKK AKEFLNYINS KLPGLLELEY EGFYLRGFFV TKKRYAVIDE EGRITTRGLE VVRRDWSEIA KETQAKVLEA ILKEGSVEKA VEVVRDVVEK IAKYRVPLEK LVIHEQITRD LKDYKAIGPH VAIAKRLAAR GIKVKPGTII SYIVLKGSGK ISDRVILLTE YDPRKHKYDP DYYIENQVLP AVLRILEAFG YRKEDLRYQS SKQTGLDAWL KR //