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P30317

- DPOL_THELI

UniProt

P30317 - DPOL_THELI

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Protein
DNA polymerase
Gene
pol
Organism
Thermococcus litoralis
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' exonuclease activity.
Intein encoded endonucleases are thought to mediate intein mobility by site-specific recombination initiated by endonuclease cleavage at the "homing site" in gene that lack the intein.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA-directed DNA polymerase activity Source: UniProtKB-KW
  3. endonuclease activity Source: UniProtKB-KW
  4. nucleotide binding Source: InterPro

GO - Biological processi

  1. intein-mediated protein splicing Source: InterPro
  2. intron homing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication, Intron homing

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase (EC:2.7.7.7)
Alternative name(s):
Vent DNA polymerase
Cleaved into the following 2 chains:
Alternative name(s):
IVPS2
Tli pol-1 intein
Alternative name(s):
IVPS1
Tli pol-2 intein
Gene namesi
Name:pol
OrganismiThermococcus litoralis
Taxonomic identifieri2265 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus

Pathology & Biotechi

Biotechnological usei

Used in the PCR method because of its high thermostability and low error rate. Sold by New England Biolabs.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 494494DNA polymerase, 1st part
PRO_0000007338Add
BLAST
Chaini495 – 1032538Endonuclease PI-TliII
PRO_0000007339Add
BLAST
Chaini1033 – 108149DNA polymerase, 2nd part
PRO_0000007340Add
BLAST
Chaini1082 – 1471390Endonuclease PI-TliI
PRO_0000007341Add
BLAST
Chaini1472 – 1702231DNA polymerase, 3rd part
PRO_0000007342Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the two intervening regions (inteins) followed by peptide ligation.

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Structurei

3D structure databases

ProteinModelPortaliP30317.
SMRiP30317. Positions 1-1082, 1456-1701.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini776 – 909134DOD-type homing endonuclease 1
Add
BLAST
Domaini1229 – 1368140DOD-type homing endonuclease 2
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di2.170.16.10. 4 hits.
3.10.28.10. 3 hits.
3.30.420.10. 1 hit.
InterProiIPR006172. DNA-dir_DNA_pol_B.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00136. DNA_pol_B. 3 hits.
PF03104. DNA_pol_B_exo1. 2 hits.
PF14528. LAGLIDADG_3. 2 hits.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00305. HintC. 2 hits.
SM00306. HintN. 2 hits.
SM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 4 hits.
SSF53098. SSF53098. 1 hit.
SSF55608. SSF55608. 2 hits.
TIGRFAMsiTIGR01443. intein_Cterm. 2 hits.
TIGR01445. intein_Nterm. 2 hits.
PROSITEiPS50818. INTEIN_C_TER. 2 hits.
PS50819. INTEIN_ENDONUCLEASE. 2 hits.
PS50817. INTEIN_N_TER. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30317-1 [UniParc]FASTAAdd to Basket

« Hide

MILDTDYITK DGKPIIRIFK KENGEFKIEL DPHFQPYIYA LLKDDSAIEE     50
IKAIKGERHG KTVRVLDAVK VRKKFLGREV EVWKLIFEHP QDVPAMRGKI 100
REHPAVVDIY EYDIPFAKRY LIDKGLIPME GDEELKLLAF DIETFYHEGD 150
EFGKGEIIMI SYADEEEARV ITWKNIDLPY VDVVSNEREM IKRFVQVVKE 200
KDPDVIITYN GDNFDLPYLI KRAEKLGVRL VLGRDKEHPE PKIQRMGDSF 250
AVEIKGRIHF DLFPVVRRTI NLPTYTLEAV YEAVLGKTKS KLGAEEIAAI 300
WETEESMKKL AQYSMEDARA TYELGKEFFP MEAELAKLIG QSVWDVSRSS 350
TGNLVEWYLL RVAYARNELA PNKPDEEEYK RRLRTTYLGG YVKEPEKGLW 400
ENIIYLDFRS LYPSIIVTHN VSPDTLEKEG CKNYDVAPIV GYRFCKDFPG 450
FIPSILGDLI AMRQDIKKKM KSTIDPIEKK MLDYRQRAIK LLANSILPNE 500
WLPIIENGEI KFVKIGEFIN SYMEKQKENV KTVENTEVLE VNNLFAFSFN 550
KKIKESEVKK VKALIRHKYK GKAYEIQLSS GRKINITAGH SLFTVRNGEI 600
KEVSGDGIKE GDLIVAPKKI KLNEKGVSIN IPELISDLSE EETADIVMTI 650
SAKGRKNFFK GMLRTLRWMF GEENRRIRTF NRYLFHLEKL GLIKLLPRGY 700
EVTDWERLKK YKQLYEKLAG SVKYNGNKRE YLVMFNEIKD FISYFPQKEL 750
EEWKIGTLNG FRTNCILKVD EDFGKLLGYY VSEGYAGAQK NKTGGISYSV 800
KLYNEDPNVL ESMKNVAEKF FGKVRVDRNC VSISKKMAYL VMKCLCGALA 850
ENKRIPSVIL TSPEPVRWSF LEAYFTGDGD IHPSKRFRLS TKSELLANQL 900
VFLLNSLGIS SVKIGFDSGV YRVYINEDLQ FPQTSREKNT YYSNLIPKEI 950
LRDVFGKEFQ KNMTFKKFKE LVDSGKLNRE KAKLLEFFIN GDIVLDRVKS 1000
VKEKDYEGYV YDLSVEDNEN FLVGFGLLYA HNSYYGYMGY PKARWYSKEC 1050
AESVTAWGRH YIEMTIREIE EKFGFKVLYA DSVSGESEII IRQNGKIRFV 1100
KIKDLFSKVD YSIGEKEYCI LEGVEALTLD DDGKLVWKPV PYVMRHRANK 1150
RMFRIWLTNS WYIDVTEDHS LIGYLNTSKT KTAKKIGERL KEVKPFELGK 1200
AVKSLICPNA PLKDENTKTS EIAVKFWELV GLIVGDGNWG GDSRWAEYYL 1250
GLSTGKDAEE IKQKLLEPLK TYGVISNYYP KNEKGDFNIL AKSLVKFMKR 1300
HFKDEKGRRK IPEFMYELPV TYIEAFLRGL FSADGTVTIR KGVPEIRLTN 1350
IDADFLREVR KLLWIVGISN SIFAETTPNR YNGVSTGTYS KHLRIKNKWR 1400
FAERIGFLIE RKQKRLLEHL KSARVKRNTI DFGFDLVHVK KVEEIPYEGY 1450
VYDIEVEETH RFFANNILVH NTDGFYATIP GEKPELIKKK AKEFLNYINS 1500
KLPGLLELEY EGFYLRGFFV TKKRYAVIDE EGRITTRGLE VVRRDWSEIA 1550
KETQAKVLEA ILKEGSVEKA VEVVRDVVEK IAKYRVPLEK LVIHEQITRD 1600
LKDYKAIGPH VAIAKRLAAR GIKVKPGTII SYIVLKGSGK ISDRVILLTE 1650
YDPRKHKYDP DYYIENQVLP AVLRILEAFG YRKEDLRYQS SKQTGLDAWL 1700
KR 1702
Length:1,702
Mass (Da):197,294
Last modified:April 1, 1993 - v1
Checksum:i21D6B98C75F53B20
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M74198 Unassigned DNA. Translation: AAA72100.1.
M74198 Unassigned DNA. Translation: AAA72101.1.
PIRiS42459.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M74198 Unassigned DNA. Translation: AAA72100.1 .
M74198 Unassigned DNA. Translation: AAA72101.1 .
PIRi S42459.

3D structure databases

ProteinModelPortali P30317.
SMRi P30317. Positions 1-1082, 1456-1701.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.170.16.10. 4 hits.
3.10.28.10. 3 hits.
3.30.420.10. 1 hit.
InterProi IPR006172. DNA-dir_DNA_pol_B.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR012337. RNaseH-like_dom.
[Graphical view ]
Pfami PF00136. DNA_pol_B. 3 hits.
PF03104. DNA_pol_B_exo1. 2 hits.
PF14528. LAGLIDADG_3. 2 hits.
[Graphical view ]
PRINTSi PR00379. INTEIN.
SMARTi SM00305. HintC. 2 hits.
SM00306. HintN. 2 hits.
SM00486. POLBc. 1 hit.
[Graphical view ]
SUPFAMi SSF51294. SSF51294. 4 hits.
SSF53098. SSF53098. 1 hit.
SSF55608. SSF55608. 2 hits.
TIGRFAMsi TIGR01443. intein_Cterm. 2 hits.
TIGR01445. intein_Nterm. 2 hits.
PROSITEi PS50818. INTEIN_C_TER. 2 hits.
PS50819. INTEIN_ENDONUCLEASE. 2 hits.
PS50817. INTEIN_N_TER. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
  2. "Protein splicing removes intervening sequences in an archaea DNA polymerase."
    Hodges R.A., Perler F.B., Noren C.J., Jack W.E.
    Nucleic Acids Res. 20:6153-6157(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SPLICING.

Entry informationi

Entry nameiDPOL_THELI
AccessioniPrimary (citable) accession number: P30317
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: September 3, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi