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Protein

DNA polymerase

Gene

pol

Organism
Thermococcus litoralis
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' exonuclease activity.
Intein encoded endonucleases are thought to mediate intein mobility by site-specific recombination initiated by endonuclease cleavage at the "homing site" in gene that lack the intein.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase
Biological processDNA replication, Intron homing

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase (EC:2.7.7.7)
Alternative name(s):
Vent DNA polymerase
Cleaved into the following 2 chains:
Alternative name(s):
IVPS2
Tli pol-1 intein
Alternative name(s):
IVPS1
Tli pol-2 intein
Gene namesi
Name:pol
OrganismiThermococcus litoralis
Taxonomic identifieri2265 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus

Pathology & Biotechi

Biotechnological usei

Used in the PCR method because of its high thermostability and low error rate. Sold by New England Biolabs.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000073381 – 494DNA polymerase, 1st partAdd BLAST494
ChainiPRO_0000007339495 – 1032Endonuclease PI-TliIIAdd BLAST538
ChainiPRO_00000073401033 – 1081DNA polymerase, 2nd partAdd BLAST49
ChainiPRO_00000073411082 – 1471Endonuclease PI-TliIAdd BLAST390
ChainiPRO_00000073421472 – 1702DNA polymerase, 3rd partAdd BLAST231

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the two intervening regions (inteins) followed by peptide ligation.

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Structurei

3D structure databases

ProteinModelPortaliP30317
SMRiP30317
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini776 – 909DOD-type homing endonuclease 1PROSITE-ProRule annotationAdd BLAST134
Domaini1229 – 1368DOD-type homing endonuclease 2PROSITE-ProRule annotationAdd BLAST140

Sequence similaritiesi

Belongs to the DNA polymerase type-B family.Curated

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di3.10.28.10, 2 hits
3.30.420.10, 1 hit
3.90.1600.10, 3 hits
InterProiView protein in InterPro
IPR006172 DNA-dir_DNA_pol_B
IPR006133 DNA-dir_DNA_pol_B_exonuc
IPR006134 DNA-dir_DNA_pol_B_multi_dom
IPR023211 DNA_pol_palm_dom_sf
IPR003586 Hint_dom_C
IPR003587 Hint_dom_N
IPR036844 Hint_dom_sf
IPR027434 Homing_endonucl
IPR006142 INTEIN
IPR030934 Intein_C
IPR004042 Intein_endonuc
IPR006141 Intein_N
IPR004860 LAGLIDADG_2
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
PfamiView protein in Pfam
PF00136 DNA_pol_B, 3 hits
PF03104 DNA_pol_B_exo1, 2 hits
PF14528 LAGLIDADG_3, 2 hits
PRINTSiPR00379 INTEIN
SMARTiView protein in SMART
SM00305 HintC, 2 hits
SM00306 HintN, 2 hits
SM00486 POLBc, 1 hit
SUPFAMiSSF51294 SSF51294, 4 hits
SSF53098 SSF53098, 1 hit
SSF55608 SSF55608, 2 hits
TIGRFAMsiTIGR01443 intein_Cterm, 2 hits
TIGR01445 intein_Nterm, 2 hits
PROSITEiView protein in PROSITE
PS50818 INTEIN_C_TER, 2 hits
PS50819 INTEIN_ENDONUCLEASE, 2 hits
PS50817 INTEIN_N_TER, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30317-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILDTDYITK DGKPIIRIFK KENGEFKIEL DPHFQPYIYA LLKDDSAIEE
60 70 80 90 100
IKAIKGERHG KTVRVLDAVK VRKKFLGREV EVWKLIFEHP QDVPAMRGKI
110 120 130 140 150
REHPAVVDIY EYDIPFAKRY LIDKGLIPME GDEELKLLAF DIETFYHEGD
160 170 180 190 200
EFGKGEIIMI SYADEEEARV ITWKNIDLPY VDVVSNEREM IKRFVQVVKE
210 220 230 240 250
KDPDVIITYN GDNFDLPYLI KRAEKLGVRL VLGRDKEHPE PKIQRMGDSF
260 270 280 290 300
AVEIKGRIHF DLFPVVRRTI NLPTYTLEAV YEAVLGKTKS KLGAEEIAAI
310 320 330 340 350
WETEESMKKL AQYSMEDARA TYELGKEFFP MEAELAKLIG QSVWDVSRSS
360 370 380 390 400
TGNLVEWYLL RVAYARNELA PNKPDEEEYK RRLRTTYLGG YVKEPEKGLW
410 420 430 440 450
ENIIYLDFRS LYPSIIVTHN VSPDTLEKEG CKNYDVAPIV GYRFCKDFPG
460 470 480 490 500
FIPSILGDLI AMRQDIKKKM KSTIDPIEKK MLDYRQRAIK LLANSILPNE
510 520 530 540 550
WLPIIENGEI KFVKIGEFIN SYMEKQKENV KTVENTEVLE VNNLFAFSFN
560 570 580 590 600
KKIKESEVKK VKALIRHKYK GKAYEIQLSS GRKINITAGH SLFTVRNGEI
610 620 630 640 650
KEVSGDGIKE GDLIVAPKKI KLNEKGVSIN IPELISDLSE EETADIVMTI
660 670 680 690 700
SAKGRKNFFK GMLRTLRWMF GEENRRIRTF NRYLFHLEKL GLIKLLPRGY
710 720 730 740 750
EVTDWERLKK YKQLYEKLAG SVKYNGNKRE YLVMFNEIKD FISYFPQKEL
760 770 780 790 800
EEWKIGTLNG FRTNCILKVD EDFGKLLGYY VSEGYAGAQK NKTGGISYSV
810 820 830 840 850
KLYNEDPNVL ESMKNVAEKF FGKVRVDRNC VSISKKMAYL VMKCLCGALA
860 870 880 890 900
ENKRIPSVIL TSPEPVRWSF LEAYFTGDGD IHPSKRFRLS TKSELLANQL
910 920 930 940 950
VFLLNSLGIS SVKIGFDSGV YRVYINEDLQ FPQTSREKNT YYSNLIPKEI
960 970 980 990 1000
LRDVFGKEFQ KNMTFKKFKE LVDSGKLNRE KAKLLEFFIN GDIVLDRVKS
1010 1020 1030 1040 1050
VKEKDYEGYV YDLSVEDNEN FLVGFGLLYA HNSYYGYMGY PKARWYSKEC
1060 1070 1080 1090 1100
AESVTAWGRH YIEMTIREIE EKFGFKVLYA DSVSGESEII IRQNGKIRFV
1110 1120 1130 1140 1150
KIKDLFSKVD YSIGEKEYCI LEGVEALTLD DDGKLVWKPV PYVMRHRANK
1160 1170 1180 1190 1200
RMFRIWLTNS WYIDVTEDHS LIGYLNTSKT KTAKKIGERL KEVKPFELGK
1210 1220 1230 1240 1250
AVKSLICPNA PLKDENTKTS EIAVKFWELV GLIVGDGNWG GDSRWAEYYL
1260 1270 1280 1290 1300
GLSTGKDAEE IKQKLLEPLK TYGVISNYYP KNEKGDFNIL AKSLVKFMKR
1310 1320 1330 1340 1350
HFKDEKGRRK IPEFMYELPV TYIEAFLRGL FSADGTVTIR KGVPEIRLTN
1360 1370 1380 1390 1400
IDADFLREVR KLLWIVGISN SIFAETTPNR YNGVSTGTYS KHLRIKNKWR
1410 1420 1430 1440 1450
FAERIGFLIE RKQKRLLEHL KSARVKRNTI DFGFDLVHVK KVEEIPYEGY
1460 1470 1480 1490 1500
VYDIEVEETH RFFANNILVH NTDGFYATIP GEKPELIKKK AKEFLNYINS
1510 1520 1530 1540 1550
KLPGLLELEY EGFYLRGFFV TKKRYAVIDE EGRITTRGLE VVRRDWSEIA
1560 1570 1580 1590 1600
KETQAKVLEA ILKEGSVEKA VEVVRDVVEK IAKYRVPLEK LVIHEQITRD
1610 1620 1630 1640 1650
LKDYKAIGPH VAIAKRLAAR GIKVKPGTII SYIVLKGSGK ISDRVILLTE
1660 1670 1680 1690 1700
YDPRKHKYDP DYYIENQVLP AVLRILEAFG YRKEDLRYQS SKQTGLDAWL

KR
Length:1,702
Mass (Da):197,294
Last modified:April 1, 1993 - v1
Checksum:i21D6B98C75F53B20
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74198 Unassigned DNA Translation: AAA72100.1
M74198 Unassigned DNA Translation: AAA72101.1
PIRiS42459

Genome annotation databases

GeneIDi16550440

Similar proteinsi

Entry informationi

Entry nameiDPOL_THELI
AccessioniPrimary (citable) accession number: P30317
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: April 25, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing
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Main funding by: National Institutes of Health