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P30317

- DPOL_THELI

UniProt

P30317 - DPOL_THELI

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Protein

DNA polymerase

Gene

pol

Organism
Thermococcus litoralis
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' exonuclease activity.
Intein encoded endonucleases are thought to mediate intein mobility by site-specific recombination initiated by endonuclease cleavage at the "homing site" in gene that lack the intein.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA-directed DNA polymerase activity Source: UniProtKB-KW
  3. endonuclease activity Source: UniProtKB-KW
  4. nucleotide binding Source: InterPro

GO - Biological processi

  1. intein-mediated protein splicing Source: InterPro
  2. intron homing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication, Intron homing

Keywords - Ligandi

DNA-binding

Protein family/group databases

MEROPSiN10.007.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase (EC:2.7.7.7)
Alternative name(s):
Vent DNA polymerase
Cleaved into the following 2 chains:
Alternative name(s):
IVPS2
Tli pol-1 intein
Alternative name(s):
IVPS1
Tli pol-2 intein
Gene namesi
Name:pol
OrganismiThermococcus litoralis
Taxonomic identifieri2265 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus

Pathology & Biotechi

Biotechnological usei

Used in the PCR method because of its high thermostability and low error rate. Sold by New England Biolabs.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 494494DNA polymerase, 1st partPRO_0000007338Add
BLAST
Chaini495 – 1032538Endonuclease PI-TliIIPRO_0000007339Add
BLAST
Chaini1033 – 108149DNA polymerase, 2nd partPRO_0000007340Add
BLAST
Chaini1082 – 1471390Endonuclease PI-TliIPRO_0000007341Add
BLAST
Chaini1472 – 1702231DNA polymerase, 3rd partPRO_0000007342Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the two intervening regions (inteins) followed by peptide ligation.

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Structurei

3D structure databases

ProteinModelPortaliP30317.
SMRiP30317. Positions 1-1082, 1456-1701.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini776 – 909134DOD-type homing endonuclease 1PROSITE-ProRule annotationAdd
BLAST
Domaini1229 – 1368140DOD-type homing endonuclease 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the DNA polymerase type-B family.Curated
Contains 2 DOD-type homing endonuclease domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di2.170.16.10. 4 hits.
3.10.28.10. 3 hits.
3.30.420.10. 1 hit.
InterProiIPR006172. DNA-dir_DNA_pol_B.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00136. DNA_pol_B. 3 hits.
PF03104. DNA_pol_B_exo1. 2 hits.
PF14528. LAGLIDADG_3. 2 hits.
[Graphical view]
PRINTSiPR00379. INTEIN.
SMARTiSM00305. HintC. 2 hits.
SM00306. HintN. 2 hits.
SM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 4 hits.
SSF53098. SSF53098. 1 hit.
SSF55608. SSF55608. 2 hits.
TIGRFAMsiTIGR01443. intein_Cterm. 2 hits.
TIGR01445. intein_Nterm. 2 hits.
PROSITEiPS50818. INTEIN_C_TER. 2 hits.
PS50819. INTEIN_ENDONUCLEASE. 2 hits.
PS50817. INTEIN_N_TER. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30317-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MILDTDYITK DGKPIIRIFK KENGEFKIEL DPHFQPYIYA LLKDDSAIEE
60 70 80 90 100
IKAIKGERHG KTVRVLDAVK VRKKFLGREV EVWKLIFEHP QDVPAMRGKI
110 120 130 140 150
REHPAVVDIY EYDIPFAKRY LIDKGLIPME GDEELKLLAF DIETFYHEGD
160 170 180 190 200
EFGKGEIIMI SYADEEEARV ITWKNIDLPY VDVVSNEREM IKRFVQVVKE
210 220 230 240 250
KDPDVIITYN GDNFDLPYLI KRAEKLGVRL VLGRDKEHPE PKIQRMGDSF
260 270 280 290 300
AVEIKGRIHF DLFPVVRRTI NLPTYTLEAV YEAVLGKTKS KLGAEEIAAI
310 320 330 340 350
WETEESMKKL AQYSMEDARA TYELGKEFFP MEAELAKLIG QSVWDVSRSS
360 370 380 390 400
TGNLVEWYLL RVAYARNELA PNKPDEEEYK RRLRTTYLGG YVKEPEKGLW
410 420 430 440 450
ENIIYLDFRS LYPSIIVTHN VSPDTLEKEG CKNYDVAPIV GYRFCKDFPG
460 470 480 490 500
FIPSILGDLI AMRQDIKKKM KSTIDPIEKK MLDYRQRAIK LLANSILPNE
510 520 530 540 550
WLPIIENGEI KFVKIGEFIN SYMEKQKENV KTVENTEVLE VNNLFAFSFN
560 570 580 590 600
KKIKESEVKK VKALIRHKYK GKAYEIQLSS GRKINITAGH SLFTVRNGEI
610 620 630 640 650
KEVSGDGIKE GDLIVAPKKI KLNEKGVSIN IPELISDLSE EETADIVMTI
660 670 680 690 700
SAKGRKNFFK GMLRTLRWMF GEENRRIRTF NRYLFHLEKL GLIKLLPRGY
710 720 730 740 750
EVTDWERLKK YKQLYEKLAG SVKYNGNKRE YLVMFNEIKD FISYFPQKEL
760 770 780 790 800
EEWKIGTLNG FRTNCILKVD EDFGKLLGYY VSEGYAGAQK NKTGGISYSV
810 820 830 840 850
KLYNEDPNVL ESMKNVAEKF FGKVRVDRNC VSISKKMAYL VMKCLCGALA
860 870 880 890 900
ENKRIPSVIL TSPEPVRWSF LEAYFTGDGD IHPSKRFRLS TKSELLANQL
910 920 930 940 950
VFLLNSLGIS SVKIGFDSGV YRVYINEDLQ FPQTSREKNT YYSNLIPKEI
960 970 980 990 1000
LRDVFGKEFQ KNMTFKKFKE LVDSGKLNRE KAKLLEFFIN GDIVLDRVKS
1010 1020 1030 1040 1050
VKEKDYEGYV YDLSVEDNEN FLVGFGLLYA HNSYYGYMGY PKARWYSKEC
1060 1070 1080 1090 1100
AESVTAWGRH YIEMTIREIE EKFGFKVLYA DSVSGESEII IRQNGKIRFV
1110 1120 1130 1140 1150
KIKDLFSKVD YSIGEKEYCI LEGVEALTLD DDGKLVWKPV PYVMRHRANK
1160 1170 1180 1190 1200
RMFRIWLTNS WYIDVTEDHS LIGYLNTSKT KTAKKIGERL KEVKPFELGK
1210 1220 1230 1240 1250
AVKSLICPNA PLKDENTKTS EIAVKFWELV GLIVGDGNWG GDSRWAEYYL
1260 1270 1280 1290 1300
GLSTGKDAEE IKQKLLEPLK TYGVISNYYP KNEKGDFNIL AKSLVKFMKR
1310 1320 1330 1340 1350
HFKDEKGRRK IPEFMYELPV TYIEAFLRGL FSADGTVTIR KGVPEIRLTN
1360 1370 1380 1390 1400
IDADFLREVR KLLWIVGISN SIFAETTPNR YNGVSTGTYS KHLRIKNKWR
1410 1420 1430 1440 1450
FAERIGFLIE RKQKRLLEHL KSARVKRNTI DFGFDLVHVK KVEEIPYEGY
1460 1470 1480 1490 1500
VYDIEVEETH RFFANNILVH NTDGFYATIP GEKPELIKKK AKEFLNYINS
1510 1520 1530 1540 1550
KLPGLLELEY EGFYLRGFFV TKKRYAVIDE EGRITTRGLE VVRRDWSEIA
1560 1570 1580 1590 1600
KETQAKVLEA ILKEGSVEKA VEVVRDVVEK IAKYRVPLEK LVIHEQITRD
1610 1620 1630 1640 1650
LKDYKAIGPH VAIAKRLAAR GIKVKPGTII SYIVLKGSGK ISDRVILLTE
1660 1670 1680 1690 1700
YDPRKHKYDP DYYIENQVLP AVLRILEAFG YRKEDLRYQS SKQTGLDAWL

KR
Length:1,702
Mass (Da):197,294
Last modified:April 1, 1993 - v1
Checksum:i21D6B98C75F53B20
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74198 Unassigned DNA. Translation: AAA72100.1.
M74198 Unassigned DNA. Translation: AAA72101.1.
PIRiS42459.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74198 Unassigned DNA. Translation: AAA72100.1 .
M74198 Unassigned DNA. Translation: AAA72101.1 .
PIRi S42459.

3D structure databases

ProteinModelPortali P30317.
SMRi P30317. Positions 1-1082, 1456-1701.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi N10.007.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.170.16.10. 4 hits.
3.10.28.10. 3 hits.
3.30.420.10. 1 hit.
InterProi IPR006172. DNA-dir_DNA_pol_B.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR012337. RNaseH-like_dom.
[Graphical view ]
Pfami PF00136. DNA_pol_B. 3 hits.
PF03104. DNA_pol_B_exo1. 2 hits.
PF14528. LAGLIDADG_3. 2 hits.
[Graphical view ]
PRINTSi PR00379. INTEIN.
SMARTi SM00305. HintC. 2 hits.
SM00306. HintN. 2 hits.
SM00486. POLBc. 1 hit.
[Graphical view ]
SUPFAMi SSF51294. SSF51294. 4 hits.
SSF53098. SSF53098. 1 hit.
SSF55608. SSF55608. 2 hits.
TIGRFAMsi TIGR01443. intein_Cterm. 2 hits.
TIGR01445. intein_Nterm. 2 hits.
PROSITEi PS50818. INTEIN_C_TER. 2 hits.
PS50819. INTEIN_ENDONUCLEASE. 2 hits.
PS50817. INTEIN_N_TER. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
  2. "Protein splicing removes intervening sequences in an archaea DNA polymerase."
    Hodges R.A., Perler F.B., Noren C.J., Jack W.E.
    Nucleic Acids Res. 20:6153-6157(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SPLICING.

Entry informationi

Entry nameiDPOL_THELI
AccessioniPrimary (citable) accession number: P30317
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: October 29, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3