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P30317

- DPOL_THELI

UniProt

P30317 - DPOL_THELI

Protein

DNA polymerase

Gene

pol

Organism
Thermococcus litoralis
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' exonuclease activity.
    Intein encoded endonucleases are thought to mediate intein mobility by site-specific recombination initiated by endonuclease cleavage at the "homing site" in gene that lack the intein.

    Catalytic activityi

    Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA-directed DNA polymerase activity Source: UniProtKB-KW
    3. endonuclease activity Source: UniProtKB-KW
    4. nucleotide binding Source: InterPro

    GO - Biological processi

    1. intein-mediated protein splicing Source: InterPro
    2. intron homing Source: UniProtKB-KW

    Keywords - Molecular functioni

    DNA-directed DNA polymerase, Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    DNA replication, Intron homing

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA polymerase (EC:2.7.7.7)
    Alternative name(s):
    Vent DNA polymerase
    Cleaved into the following 2 chains:
    Alternative name(s):
    IVPS2
    Tli pol-1 intein
    Alternative name(s):
    IVPS1
    Tli pol-2 intein
    Gene namesi
    Name:pol
    OrganismiThermococcus litoralis
    Taxonomic identifieri2265 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus

    Pathology & Biotechi

    Biotechnological usei

    Used in the PCR method because of its high thermostability and low error rate. Sold by New England Biolabs.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 494494DNA polymerase, 1st partPRO_0000007338Add
    BLAST
    Chaini495 – 1032538Endonuclease PI-TliIIPRO_0000007339Add
    BLAST
    Chaini1033 – 108149DNA polymerase, 2nd partPRO_0000007340Add
    BLAST
    Chaini1082 – 1471390Endonuclease PI-TliIPRO_0000007341Add
    BLAST
    Chaini1472 – 1702231DNA polymerase, 3rd partPRO_0000007342Add
    BLAST

    Post-translational modificationi

    This protein undergoes a protein self splicing that involves a post-translational excision of the two intervening regions (inteins) followed by peptide ligation.

    Keywords - PTMi

    Autocatalytic cleavage, Protein splicing

    Structurei

    3D structure databases

    ProteinModelPortaliP30317.
    SMRiP30317. Positions 1-1082, 1456-1701.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini776 – 909134DOD-type homing endonuclease 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini1229 – 1368140DOD-type homing endonuclease 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the DNA polymerase type-B family.Curated
    Contains 2 DOD-type homing endonuclease domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Family and domain databases

    Gene3Di2.170.16.10. 4 hits.
    3.10.28.10. 3 hits.
    3.30.420.10. 1 hit.
    InterProiIPR006172. DNA-dir_DNA_pol_B.
    IPR006133. DNA-dir_DNA_pol_B_exonuc.
    IPR006134. DNA-dir_DNA_pol_B_multi_dom.
    IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR012337. RNaseH-like_dom.
    [Graphical view]
    PfamiPF00136. DNA_pol_B. 3 hits.
    PF03104. DNA_pol_B_exo1. 2 hits.
    PF14528. LAGLIDADG_3. 2 hits.
    [Graphical view]
    PRINTSiPR00379. INTEIN.
    SMARTiSM00305. HintC. 2 hits.
    SM00306. HintN. 2 hits.
    SM00486. POLBc. 1 hit.
    [Graphical view]
    SUPFAMiSSF51294. SSF51294. 4 hits.
    SSF53098. SSF53098. 1 hit.
    SSF55608. SSF55608. 2 hits.
    TIGRFAMsiTIGR01443. intein_Cterm. 2 hits.
    TIGR01445. intein_Nterm. 2 hits.
    PROSITEiPS50818. INTEIN_C_TER. 2 hits.
    PS50819. INTEIN_ENDONUCLEASE. 2 hits.
    PS50817. INTEIN_N_TER. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P30317-1 [UniParc]FASTAAdd to Basket

    « Hide

    MILDTDYITK DGKPIIRIFK KENGEFKIEL DPHFQPYIYA LLKDDSAIEE     50
    IKAIKGERHG KTVRVLDAVK VRKKFLGREV EVWKLIFEHP QDVPAMRGKI 100
    REHPAVVDIY EYDIPFAKRY LIDKGLIPME GDEELKLLAF DIETFYHEGD 150
    EFGKGEIIMI SYADEEEARV ITWKNIDLPY VDVVSNEREM IKRFVQVVKE 200
    KDPDVIITYN GDNFDLPYLI KRAEKLGVRL VLGRDKEHPE PKIQRMGDSF 250
    AVEIKGRIHF DLFPVVRRTI NLPTYTLEAV YEAVLGKTKS KLGAEEIAAI 300
    WETEESMKKL AQYSMEDARA TYELGKEFFP MEAELAKLIG QSVWDVSRSS 350
    TGNLVEWYLL RVAYARNELA PNKPDEEEYK RRLRTTYLGG YVKEPEKGLW 400
    ENIIYLDFRS LYPSIIVTHN VSPDTLEKEG CKNYDVAPIV GYRFCKDFPG 450
    FIPSILGDLI AMRQDIKKKM KSTIDPIEKK MLDYRQRAIK LLANSILPNE 500
    WLPIIENGEI KFVKIGEFIN SYMEKQKENV KTVENTEVLE VNNLFAFSFN 550
    KKIKESEVKK VKALIRHKYK GKAYEIQLSS GRKINITAGH SLFTVRNGEI 600
    KEVSGDGIKE GDLIVAPKKI KLNEKGVSIN IPELISDLSE EETADIVMTI 650
    SAKGRKNFFK GMLRTLRWMF GEENRRIRTF NRYLFHLEKL GLIKLLPRGY 700
    EVTDWERLKK YKQLYEKLAG SVKYNGNKRE YLVMFNEIKD FISYFPQKEL 750
    EEWKIGTLNG FRTNCILKVD EDFGKLLGYY VSEGYAGAQK NKTGGISYSV 800
    KLYNEDPNVL ESMKNVAEKF FGKVRVDRNC VSISKKMAYL VMKCLCGALA 850
    ENKRIPSVIL TSPEPVRWSF LEAYFTGDGD IHPSKRFRLS TKSELLANQL 900
    VFLLNSLGIS SVKIGFDSGV YRVYINEDLQ FPQTSREKNT YYSNLIPKEI 950
    LRDVFGKEFQ KNMTFKKFKE LVDSGKLNRE KAKLLEFFIN GDIVLDRVKS 1000
    VKEKDYEGYV YDLSVEDNEN FLVGFGLLYA HNSYYGYMGY PKARWYSKEC 1050
    AESVTAWGRH YIEMTIREIE EKFGFKVLYA DSVSGESEII IRQNGKIRFV 1100
    KIKDLFSKVD YSIGEKEYCI LEGVEALTLD DDGKLVWKPV PYVMRHRANK 1150
    RMFRIWLTNS WYIDVTEDHS LIGYLNTSKT KTAKKIGERL KEVKPFELGK 1200
    AVKSLICPNA PLKDENTKTS EIAVKFWELV GLIVGDGNWG GDSRWAEYYL 1250
    GLSTGKDAEE IKQKLLEPLK TYGVISNYYP KNEKGDFNIL AKSLVKFMKR 1300
    HFKDEKGRRK IPEFMYELPV TYIEAFLRGL FSADGTVTIR KGVPEIRLTN 1350
    IDADFLREVR KLLWIVGISN SIFAETTPNR YNGVSTGTYS KHLRIKNKWR 1400
    FAERIGFLIE RKQKRLLEHL KSARVKRNTI DFGFDLVHVK KVEEIPYEGY 1450
    VYDIEVEETH RFFANNILVH NTDGFYATIP GEKPELIKKK AKEFLNYINS 1500
    KLPGLLELEY EGFYLRGFFV TKKRYAVIDE EGRITTRGLE VVRRDWSEIA 1550
    KETQAKVLEA ILKEGSVEKA VEVVRDVVEK IAKYRVPLEK LVIHEQITRD 1600
    LKDYKAIGPH VAIAKRLAAR GIKVKPGTII SYIVLKGSGK ISDRVILLTE 1650
    YDPRKHKYDP DYYIENQVLP AVLRILEAFG YRKEDLRYQS SKQTGLDAWL 1700
    KR 1702
    Length:1,702
    Mass (Da):197,294
    Last modified:April 1, 1993 - v1
    Checksum:i21D6B98C75F53B20
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M74198 Unassigned DNA. Translation: AAA72100.1.
    M74198 Unassigned DNA. Translation: AAA72101.1.
    PIRiS42459.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M74198 Unassigned DNA. Translation: AAA72100.1 .
    M74198 Unassigned DNA. Translation: AAA72101.1 .
    PIRi S42459.

    3D structure databases

    ProteinModelPortali P30317.
    SMRi P30317. Positions 1-1082, 1456-1701.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.170.16.10. 4 hits.
    3.10.28.10. 3 hits.
    3.30.420.10. 1 hit.
    InterProi IPR006172. DNA-dir_DNA_pol_B.
    IPR006133. DNA-dir_DNA_pol_B_exonuc.
    IPR006134. DNA-dir_DNA_pol_B_multi_dom.
    IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR012337. RNaseH-like_dom.
    [Graphical view ]
    Pfami PF00136. DNA_pol_B. 3 hits.
    PF03104. DNA_pol_B_exo1. 2 hits.
    PF14528. LAGLIDADG_3. 2 hits.
    [Graphical view ]
    PRINTSi PR00379. INTEIN.
    SMARTi SM00305. HintC. 2 hits.
    SM00306. HintN. 2 hits.
    SM00486. POLBc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51294. SSF51294. 4 hits.
    SSF53098. SSF53098. 1 hit.
    SSF55608. SSF55608. 2 hits.
    TIGRFAMsi TIGR01443. intein_Cterm. 2 hits.
    TIGR01445. intein_Nterm. 2 hits.
    PROSITEi PS50818. INTEIN_C_TER. 2 hits.
    PS50819. INTEIN_ENDONUCLEASE. 2 hits.
    PS50817. INTEIN_N_TER. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    2. "Protein splicing removes intervening sequences in an archaea DNA polymerase."
      Hodges R.A., Perler F.B., Noren C.J., Jack W.E.
      Nucleic Acids Res. 20:6153-6157(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SPLICING.

    Entry informationi

    Entry nameiDPOL_THELI
    AccessioniPrimary (citable) accession number: P30317
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Intein-containing proteins
      List of intein-containing protein entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3