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P30317 (DPOL_THELI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA polymerase

EC=2.7.7.7
Alternative name(s):
Vent DNA polymerase

Cleaved into the following 2 chains:

  1. Endonuclease PI-TliII
    EC=3.1.-.-
    Alternative name(s):
    IVPS2
    Tli pol-1 intein
  2. Endonuclease PI-TliI
    EC=3.1.-.-
    Alternative name(s):
    IVPS1
    Tli pol-2 intein
Gene names
Name:pol
OrganismThermococcus litoralis
Taxonomic identifier2265 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus

Protein attributes

Sequence length1702 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' exonuclease activity.

Intein encoded endonucleases are thought to mediate intein mobility by site-specific recombination initiated by endonuclease cleavage at the "homing site" in gene that lack the intein.

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the two intervening regions (inteins) followed by peptide ligation.

Biotechnological use

Used in the PCR method because of its high thermostability and low error rate. Sold by New England Biolabs.

Sequence similarities

Belongs to the DNA polymerase type-B family.

Contains 2 DOD-type homing endonuclease domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 494494DNA polymerase, 1st part
PRO_0000007338
Chain495 – 1032538Endonuclease PI-TliII
PRO_0000007339
Chain1033 – 108149DNA polymerase, 2nd part
PRO_0000007340
Chain1082 – 1471390Endonuclease PI-TliI
PRO_0000007341
Chain1472 – 1702231DNA polymerase, 3rd part
PRO_0000007342

Regions

Domain776 – 909134DOD-type homing endonuclease 1
Domain1229 – 1368140DOD-type homing endonuclease 2

Sequences

Sequence LengthMass (Da)Tools
P30317 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 21D6B98C75F53B20

FASTA1,702197,294
        10         20         30         40         50         60 
MILDTDYITK DGKPIIRIFK KENGEFKIEL DPHFQPYIYA LLKDDSAIEE IKAIKGERHG 

        70         80         90        100        110        120 
KTVRVLDAVK VRKKFLGREV EVWKLIFEHP QDVPAMRGKI REHPAVVDIY EYDIPFAKRY 

       130        140        150        160        170        180 
LIDKGLIPME GDEELKLLAF DIETFYHEGD EFGKGEIIMI SYADEEEARV ITWKNIDLPY 

       190        200        210        220        230        240 
VDVVSNEREM IKRFVQVVKE KDPDVIITYN GDNFDLPYLI KRAEKLGVRL VLGRDKEHPE 

       250        260        270        280        290        300 
PKIQRMGDSF AVEIKGRIHF DLFPVVRRTI NLPTYTLEAV YEAVLGKTKS KLGAEEIAAI 

       310        320        330        340        350        360 
WETEESMKKL AQYSMEDARA TYELGKEFFP MEAELAKLIG QSVWDVSRSS TGNLVEWYLL 

       370        380        390        400        410        420 
RVAYARNELA PNKPDEEEYK RRLRTTYLGG YVKEPEKGLW ENIIYLDFRS LYPSIIVTHN 

       430        440        450        460        470        480 
VSPDTLEKEG CKNYDVAPIV GYRFCKDFPG FIPSILGDLI AMRQDIKKKM KSTIDPIEKK 

       490        500        510        520        530        540 
MLDYRQRAIK LLANSILPNE WLPIIENGEI KFVKIGEFIN SYMEKQKENV KTVENTEVLE 

       550        560        570        580        590        600 
VNNLFAFSFN KKIKESEVKK VKALIRHKYK GKAYEIQLSS GRKINITAGH SLFTVRNGEI 

       610        620        630        640        650        660 
KEVSGDGIKE GDLIVAPKKI KLNEKGVSIN IPELISDLSE EETADIVMTI SAKGRKNFFK 

       670        680        690        700        710        720 
GMLRTLRWMF GEENRRIRTF NRYLFHLEKL GLIKLLPRGY EVTDWERLKK YKQLYEKLAG 

       730        740        750        760        770        780 
SVKYNGNKRE YLVMFNEIKD FISYFPQKEL EEWKIGTLNG FRTNCILKVD EDFGKLLGYY 

       790        800        810        820        830        840 
VSEGYAGAQK NKTGGISYSV KLYNEDPNVL ESMKNVAEKF FGKVRVDRNC VSISKKMAYL 

       850        860        870        880        890        900 
VMKCLCGALA ENKRIPSVIL TSPEPVRWSF LEAYFTGDGD IHPSKRFRLS TKSELLANQL 

       910        920        930        940        950        960 
VFLLNSLGIS SVKIGFDSGV YRVYINEDLQ FPQTSREKNT YYSNLIPKEI LRDVFGKEFQ 

       970        980        990       1000       1010       1020 
KNMTFKKFKE LVDSGKLNRE KAKLLEFFIN GDIVLDRVKS VKEKDYEGYV YDLSVEDNEN 

      1030       1040       1050       1060       1070       1080 
FLVGFGLLYA HNSYYGYMGY PKARWYSKEC AESVTAWGRH YIEMTIREIE EKFGFKVLYA 

      1090       1100       1110       1120       1130       1140 
DSVSGESEII IRQNGKIRFV KIKDLFSKVD YSIGEKEYCI LEGVEALTLD DDGKLVWKPV 

      1150       1160       1170       1180       1190       1200 
PYVMRHRANK RMFRIWLTNS WYIDVTEDHS LIGYLNTSKT KTAKKIGERL KEVKPFELGK 

      1210       1220       1230       1240       1250       1260 
AVKSLICPNA PLKDENTKTS EIAVKFWELV GLIVGDGNWG GDSRWAEYYL GLSTGKDAEE 

      1270       1280       1290       1300       1310       1320 
IKQKLLEPLK TYGVISNYYP KNEKGDFNIL AKSLVKFMKR HFKDEKGRRK IPEFMYELPV 

      1330       1340       1350       1360       1370       1380 
TYIEAFLRGL FSADGTVTIR KGVPEIRLTN IDADFLREVR KLLWIVGISN SIFAETTPNR 

      1390       1400       1410       1420       1430       1440 
YNGVSTGTYS KHLRIKNKWR FAERIGFLIE RKQKRLLEHL KSARVKRNTI DFGFDLVHVK 

      1450       1460       1470       1480       1490       1500 
KVEEIPYEGY VYDIEVEETH RFFANNILVH NTDGFYATIP GEKPELIKKK AKEFLNYINS 

      1510       1520       1530       1540       1550       1560 
KLPGLLELEY EGFYLRGFFV TKKRYAVIDE EGRITTRGLE VVRRDWSEIA KETQAKVLEA 

      1570       1580       1590       1600       1610       1620 
ILKEGSVEKA VEVVRDVVEK IAKYRVPLEK LVIHEQITRD LKDYKAIGPH VAIAKRLAAR 

      1630       1640       1650       1660       1670       1680 
GIKVKPGTII SYIVLKGSGK ISDRVILLTE YDPRKHKYDP DYYIENQVLP AVLRILEAFG 

      1690       1700 
YRKEDLRYQS SKQTGLDAWL KR 

« Hide

References

[1]"Intervening sequences in an Archaea DNA polymerase gene."
Perler F.B., Comb D.G., Jack W.E., Moran L.S., Qiang B., Kucera R.B., Benner J., Slatko B.E., Nwankwo D.O., Hempstead S.K., Carlow C.K.S., Jannasch H.
Proc. Natl. Acad. Sci. U.S.A. 89:5577-5581(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]"Protein splicing removes intervening sequences in an archaea DNA polymerase."
Hodges R.A., Perler F.B., Noren C.J., Jack W.E.
Nucleic Acids Res. 20:6153-6157(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SPLICING.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M74198 Unassigned DNA. Translation: AAA72100.1.
M74198 Unassigned DNA. Translation: AAA72101.1.
PIRS42459.

3D structure databases

ProteinModelPortalP30317.
SMRP30317. Positions 1-1082, 1456-1701.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.170.16.10. 4 hits.
3.10.28.10. 3 hits.
3.30.420.10. 1 hit.
InterProIPR006172. DNA-dir_DNA_pol_B.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR004578. DNA-dir_DNA_pol_B_pol2.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamPF00136. DNA_pol_B. 3 hits.
PF03104. DNA_pol_B_exo1. 2 hits.
PF14528. LAGLIDADG_3. 2 hits.
[Graphical view]
PRINTSPR00379. INTEIN.
SMARTSM00305. HintC. 2 hits.
SM00306. HintN. 2 hits.
SM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMSSF51294. SSF51294. 4 hits.
SSF53098. SSF53098. 1 hit.
SSF55608. SSF55608. 2 hits.
TIGRFAMsTIGR01443. intein_Cterm. 2 hits.
TIGR01445. intein_Nterm. 2 hits.
TIGR00592. pol2. 2 hits.
PROSITEPS50818. INTEIN_C_TER. 2 hits.
PS50819. INTEIN_ENDONUCLEASE. 2 hits.
PS50817. INTEIN_N_TER. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDPOL_THELI
AccessionPrimary (citable) accession number: P30317
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: June 11, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Intein-containing proteins

List of intein-containing protein entries