M-phase inducer phosphatase 3 - Xenopus laevis (African clawed frog)

Preferred order of sections

Names and origin

Protein names

Recommended name:
M-phase inducer phosphatase 3
EC=3.1.3.48

Gene names

Name:

cdc25-3

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	572 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function

This protein functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It may directly dephosphorylate p34(cdc2) and activate the p34(cdc2) kinase activity.

Catalytic activity

Protein tyrosine phosphate + H₂O = protein tyrosine + phosphate.

Sequence similarities

Belongs to the MPI phosphatase family.

Contains 1 rhodanese domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Molecular function	Hydrolase Protein phosphatase
Technical term	3D-structure
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 572

572

M-phase inducer phosphatase 3

PRO_0000198654

Regions

Domain

420 – 527

108

Rhodanese

Sites

Active site

476

1

By similarity

Secondary structure

1

.

572

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P30311 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: B268EA4BAA3971AC
Show »

FASTA

572

64,413

        10         20         30         40         50         60 
MIKERVTPPF QASGGSGVVM AESHIISSEA PPKSNPGLNI RTNCRMILNL LREKDCSVTF 

        70         80         90        100        110        120 
SPEQPLTPVT DLAVGFSNLS TFSGETPKRC LDLSNLGDET APLPTESPDR MSSGKLESPK 

       130        140        150        160        170        180 
TQFVQFDGLF TPDLAWKAKK CPKRNMNSVL PHLLCSTPSF KKASGGQRSL SNKENEGELF 

       190        200        210        220        230        240 
KNPNCKPVAL LLPQEVVDSQ LSPTPENKVD ISLDEDCEMN ILGSPISADP PCLDGAHDDI 

       250        260        270        280        290        300 
KMQNLDGFAD FFSVDEEEME NPPGAVGNLS CSMAILLSGP LLNQDVEISN VNNISLNRSR 

       310        320        330        340        350        360 
LYRSPSMPEK LDRPMLKRPV RPLNSETPVR VKRRRSTSSP LQPEEENCQP QRRGTSLKKT 

       370        380        390        400        410        420 
LSLCDVDISS VLDEDCGHRQ LIGDFSKVYA LPTVTGRHQD LRYITGETLA ALMHGDFNSL 

       430        440        450        460        470        480 
VEKFFIIDCR YPYEYDGGHI KSAFNLHRQD EVTDYFLQQP LTPLMAQKRL IIIFHCEFSS 

       490        500        510        520        530        540 
ERGPKMCRSL REEDRASNDY PSLYYPELYL LKGGYKDFFP EYKELCEPQS YCPMHHQDFR 

       550        560        570 
EDLLKFRTKC KTSVGDRKRR EPEFRLTGQR LG

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References

[1]	"Regulation of the cdc25 protein during the cell cycle in Xenopus extracts." Kumagai A., Dunphy W.G. Cell 70:139-151(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M94264 mRNA. Translation: AAA49675.1.

PIR

C42679.

RefSeq

NP_001081256.1. NM_001087787.1.

UniGene

Xl.85437.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1I8G	NMR	-	A	63-72	[»]

ProteinModelPortal

P30311.

SMR

P30311. Positions 381-541.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

397737.

KEGG

xla:397737.

Organism-specific databases

CTD

397737.

Xenbase

XB-GENE-6252614. cdc25c.

Phylogenomic databases

HOVERGEN

HBG052501.

KO

K05867.

Family and domain databases

Gene3D

3.40.250.10. 1 hit.

InterPro

IPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view]

PANTHER

PTHR10828. PTHR10828. 1 hit.

Pfam

PF06617. M-inducer_phosp. 1 hit.
PF00581. Rhodanese. 1 hit.
[Graphical view]

PRINTS

PR00716. MPIPHPHTASE.

SMART

SM00450. RHOD. 1 hit.
[Graphical view]

SUPFAM

SSF52821. Rhodanese-like. 1 hit.

PROSITE

PS50206. RHODANESE_3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P30311.

Entry information

Entry name

MPIP3_XENLA

Accession

Primary (citable) accession number: P30311

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	April 3, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families