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Protein

M-phase inducer phosphatase 2

Gene

Cdc25b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity (By similarity).By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Enzyme regulationi

Stimulated by B-type cyclins.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei483By similarity1

GO - Molecular functioni

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • female meiosis I Source: MGI
  • mitotic nuclear division Source: UniProtKB-KW
  • oocyte maturation Source: MGI
  • positive regulation of cytokinesis Source: MGI
  • positive regulation of mitotic cell cycle Source: MGI
  • positive regulation of protein kinase activity Source: MGI
  • protein phosphorylation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-MMU-69273. Cyclin A/B1 associated events during G2/M transition.

Names & Taxonomyi

Protein namesi
Recommended name:
M-phase inducer phosphatase 2 (EC:3.1.3.48)
Alternative name(s):
Dual specificity phosphatase Cdc25B
Gene namesi
Name:Cdc25b
Synonyms:Cdc25m2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:99701. Cdc25b.

Subcellular locationi

GO - Cellular componenti

  • centrosome Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • nucleoplasm Source: MGI
  • nucleus Source: UniProtKB
  • spindle pole Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2723.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001986451 – 576M-phase inducer phosphatase 2Add BLAST576

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei42PhosphoserineBy similarity1
Modified residuei167Phosphoserine; by MELKBy similarity1
Modified residuei248PhosphoserineBy similarity1
Modified residuei321Phosphoserine; by MELK and MAPK14By similarity1
Modified residuei351Phosphoserine; by AURKABy similarity1
Modified residuei372Phosphoserine; by BRSK1 and MAPK14By similarity1
Modified residuei559PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-351 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-167 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-321 and Ser-372 by MAPK14 is required for binding to 14-3-3 proteins (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP30306.
PaxDbiP30306.
PRIDEiP30306.

PTM databases

iPTMnetiP30306.
PhosphoSitePlusiP30306.

Expressioni

Tissue specificityi

Expressed predominantly in spleen, lung, heart, brain, intestine, and muscle.

Developmental stagei

Detected at the one-cell stage followed by a decrease in signal intensity at the two-cell stage. Detectable at higher level in the four-cell stage and expressed through the eight-cell, 16-cell and morula stages. Maximal expression at the blastocyst stage.

Gene expression databases

BgeeiENSMUSG00000027330.
CleanExiMM_CDC25B.
ExpressionAtlasiP30306. baseline and differential.
GenevisibleiP30306. MM.

Interactioni

Subunit structurei

Interacts with MAPK14 and 14-3-3 proteins.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198622. 2 interactors.
IntActiP30306. 3 interactors.
STRINGi10090.ENSMUSP00000028804.

Chemistry databases

BindingDBiP30306.

Structurei

3D structure databases

ProteinModelPortaliP30306.
SMRiP30306.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini427 – 534RhodanesePROSITE-ProRule annotationAdd BLAST108

Sequence similaritiesi

Belongs to the MPI phosphatase family.Curated
Contains 1 rhodanese domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3772. Eukaryota.
COG5105. LUCA.
GeneTreeiENSGT00390000018747.
HOGENOMiHOG000082672.
HOVERGENiHBG052501.
InParanoidiP30306.
KOiK05866.
OMAiITPCSLD.
OrthoDBiEOG091G0H0D.
PhylomeDBiP30306.
TreeFamiTF101056.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiIPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view]
PfamiPF06617. M-inducer_phosp. 1 hit.
PF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSiPR00716. MPIPHPHTASE.
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30306-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVPLQKSAP GSALSPARVL GGIQRPRHLS VFEFESDGFL GSPEPTASSS
60 70 80 90 100
PVTTLTQTMH NLAGLGSEPP KAQVGSLSFQ NRLADLSLSR RTSECSLSSE
110 120 130 140 150
SSESSDAGLC MDSPSPVDPQ MAERTFEQAI QAASRVIQNE QFTIKRFRSL
160 170 180 190 200
PVRLLEHSPV LQSITNSRAL DSWRKTEAGY RAAANSPGED KENDGYIFKM
210 220 230 240 250
PQELPHSSSA QALAEWVSRR QAFTQRPSSA PDLMCLTTEW KMEVEELSPV
260 270 280 290 300
AQSSSLTPVE RASEEDDGFV DILESDLKDD EKVPAGMENL ISAPLVKKLD
310 320 330 340 350
KEEEQDLIMF SKCQRLFRSP SMPCSVIRPI LKRLERPQDR DVPVQSKRRK
360 370 380 390 400
SVTPLEEQQL EEPKARVFRS KSLCHEIENI LDSDHRGLIG DYSKAFLLQT
410 420 430 440 450
VDGKHQDLKY ISPETMVALL TGKFSNIVEK FVIVDCRYPY EYEGGHIKNA
460 470 480 490 500
VNLPLERDAE TFLLQRPIMP CSLDKRIILI FHCEFSSERG PRMCRFIRER
510 520 530 540 550
DRAANDYPSL YYPEMYILKG GYKEFFPQHP NFCEPQDYRP MNHEAFRDEL
560 570
RNFRLKTRSW AGERSRRELC SRLQDQ
Length:576
Mass (Da):65,490
Last modified:April 1, 1993 - v1
Checksum:i5086B676581CD570
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti532F → L in AAH02287 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S93521 mRNA. Translation: AAB22026.1.
BC002287 mRNA. Translation: AAH02287.1.
CCDSiCCDS16758.1.
PIRiA42236.
RefSeqiNP_075606.1. NM_023117.4.
UniGeneiMm.38444.

Genome annotation databases

EnsembliENSMUST00000028804; ENSMUSP00000028804; ENSMUSG00000027330.
GeneIDi12531.
KEGGimmu:12531.
UCSCiuc008mky.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S93521 mRNA. Translation: AAB22026.1.
BC002287 mRNA. Translation: AAH02287.1.
CCDSiCCDS16758.1.
PIRiA42236.
RefSeqiNP_075606.1. NM_023117.4.
UniGeneiMm.38444.

3D structure databases

ProteinModelPortaliP30306.
SMRiP30306.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198622. 2 interactors.
IntActiP30306. 3 interactors.
STRINGi10090.ENSMUSP00000028804.

Chemistry databases

BindingDBiP30306.
ChEMBLiCHEMBL2723.

PTM databases

iPTMnetiP30306.
PhosphoSitePlusiP30306.

Proteomic databases

EPDiP30306.
PaxDbiP30306.
PRIDEiP30306.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028804; ENSMUSP00000028804; ENSMUSG00000027330.
GeneIDi12531.
KEGGimmu:12531.
UCSCiuc008mky.3. mouse.

Organism-specific databases

CTDi994.
MGIiMGI:99701. Cdc25b.

Phylogenomic databases

eggNOGiKOG3772. Eukaryota.
COG5105. LUCA.
GeneTreeiENSGT00390000018747.
HOGENOMiHOG000082672.
HOVERGENiHBG052501.
InParanoidiP30306.
KOiK05866.
OMAiITPCSLD.
OrthoDBiEOG091G0H0D.
PhylomeDBiP30306.
TreeFamiTF101056.

Enzyme and pathway databases

ReactomeiR-MMU-69273. Cyclin A/B1 associated events during G2/M transition.

Miscellaneous databases

PROiP30306.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027330.
CleanExiMM_CDC25B.
ExpressionAtlasiP30306. baseline and differential.
GenevisibleiP30306. MM.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiIPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view]
PfamiPF06617. M-inducer_phosp. 1 hit.
PF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSiPR00716. MPIPHPHTASE.
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMPIP2_MOUSE
AccessioniPrimary (citable) accession number: P30306
Secondary accession number(s): Q99LP3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: November 2, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.