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P30306 (MPIP2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
M-phase inducer phosphatase 2

EC=3.1.3.48
Alternative name(s):
Dual specificity phosphatase Cdc25B
Gene names
Name:Cdc25b
Synonyms:Cdc25m2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length576 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity By similarity.

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Enzyme regulation

Stimulated by B-type cyclins.

Subunit structure

Interacts with MAPK14 and 14-3-3 proteins By similarity.

Subcellular location

Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Cytoplasmcytoskeletonspindle pole By similarity.

Tissue specificity

Expressed predominantly in spleen, lung, heart, brain, intestine, and muscle.

Developmental stage

Detected at the one-cell stage followed by a decrease in signal intensity at the two-cell stage. Detectable at higher level in the four-cell stage and expressed through the eight-cell, 16-cell and morula stages. Maximal expression at the blastocyst stage.

Post-translational modification

Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-351 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-167 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-321 and Ser-372 by MAPK14 is required for binding to 14-3-3 proteins By similarity.

Sequence similarities

Belongs to the MPI phosphatase family.

Contains 1 rhodanese domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 576576M-phase inducer phosphatase 2
PRO_0000198645

Regions

Domain427 – 534108Rhodanese

Sites

Active site4831 By similarity

Amino acid modifications

Modified residue1671Phosphoserine; by MELK By similarity
Modified residue2481Phosphoserine By similarity
Modified residue3211Phosphoserine; by MELK and MAPK14 By similarity
Modified residue3511Phosphoserine; by AURKA By similarity
Modified residue3721Phosphoserine; by BRSK1 and MAPK14 By similarity

Experimental info

Sequence conflict5321F → L in AAH02287. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P30306 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 5086B676581CD570

FASTA57665,490
        10         20         30         40         50         60 
MEVPLQKSAP GSALSPARVL GGIQRPRHLS VFEFESDGFL GSPEPTASSS PVTTLTQTMH 

        70         80         90        100        110        120 
NLAGLGSEPP KAQVGSLSFQ NRLADLSLSR RTSECSLSSE SSESSDAGLC MDSPSPVDPQ 

       130        140        150        160        170        180 
MAERTFEQAI QAASRVIQNE QFTIKRFRSL PVRLLEHSPV LQSITNSRAL DSWRKTEAGY 

       190        200        210        220        230        240 
RAAANSPGED KENDGYIFKM PQELPHSSSA QALAEWVSRR QAFTQRPSSA PDLMCLTTEW 

       250        260        270        280        290        300 
KMEVEELSPV AQSSSLTPVE RASEEDDGFV DILESDLKDD EKVPAGMENL ISAPLVKKLD 

       310        320        330        340        350        360 
KEEEQDLIMF SKCQRLFRSP SMPCSVIRPI LKRLERPQDR DVPVQSKRRK SVTPLEEQQL 

       370        380        390        400        410        420 
EEPKARVFRS KSLCHEIENI LDSDHRGLIG DYSKAFLLQT VDGKHQDLKY ISPETMVALL 

       430        440        450        460        470        480 
TGKFSNIVEK FVIVDCRYPY EYEGGHIKNA VNLPLERDAE TFLLQRPIMP CSLDKRIILI 

       490        500        510        520        530        540 
FHCEFSSERG PRMCRFIRER DRAANDYPSL YYPEMYILKG GYKEFFPQHP NFCEPQDYRP 

       550        560        570 
MNHEAFRDEL RNFRLKTRSW AGERSRRELC SRLQDQ 

« Hide

References

« Hide 'large scale' references
[1]"A mouse cdc25 homolog is differentially and developmentally expressed."
Kakizuka A., Sebastian B., Borgmeyer U., Hermans-Borgmeyer I., Bolado J., Hunter T., Hoekstra M.F., Evans R.M.
Genes Dev. 6:578-590(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 328-576.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S93521 mRNA. Translation: AAB22026.1.
BC002287 mRNA. Translation: AAH02287.1.
PIRA42236.
RefSeqNP_075606.1. NM_023117.4.
UniGeneMm.38444.

3D structure databases

ProteinModelPortalP30306.
SMRP30306. Positions 383-560.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid198622. 2 interactions.
IntActP30306. 3 interactions.

Chemistry

BindingDBP30306.
ChEMBLCHEMBL2723.

PTM databases

PhosphoSiteP30306.

Proteomic databases

PRIDEP30306.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000028804; ENSMUSP00000028804; ENSMUSG00000027330.
GeneID12531.
KEGGmmu:12531.
UCSCuc008mky.3. mouse.

Organism-specific databases

CTD994.
MGIMGI:99701. Cdc25b.

Phylogenomic databases

eggNOGCOG5105.
HOGENOMHOG000082672.
HOVERGENHBG052501.
InParanoidP30306.
KOK05866.
OMATPKSQVG.
OrthoDBEOG7288R1.
PhylomeDBP30306.
TreeFamTF101056.

Gene expression databases

ArrayExpressP30306.
BgeeP30306.
CleanExMM_CDC25B.
GenevestigatorP30306.

Family and domain databases

Gene3D3.40.250.10. 1 hit.
InterProIPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view]
PfamPF06617. M-inducer_phosp. 1 hit.
PF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSPR00716. MPIPHPHTASE.
SMARTSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMSSF52821. SSF52821. 1 hit.
PROSITEPS50206. RHODANESE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio281562.
PROP30306.
SOURCESearch...

Entry information

Entry nameMPIP2_MOUSE
AccessionPrimary (citable) accession number: P30306
Secondary accession number(s): Q99LP3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: April 16, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot