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P30306

- MPIP2_MOUSE

UniProt

P30306 - MPIP2_MOUSE

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Protein

M-phase inducer phosphatase 2

Gene

Cdc25b

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity (By similarity).By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Enzyme regulationi

Stimulated by B-type cyclins.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei483 – 4831By similarity

GO - Molecular functioni

  1. protein tyrosine phosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. female meiosis I Source: MGI
  2. mitotic nuclear division Source: UniProtKB-KW
  3. oocyte maturation Source: MGI
  4. positive regulation of cytokinesis Source: Ensembl
  5. positive regulation of mitotic cell cycle Source: Ensembl
  6. positive regulation of protein kinase activity Source: MGI
  7. protein phosphorylation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiREACT_206803. Cyclin A/B1 associated events during G2/M transition.
REACT_209377. Cyclin B2 mediated events.

Names & Taxonomyi

Protein namesi
Recommended name:
M-phase inducer phosphatase 2 (EC:3.1.3.48)
Alternative name(s):
Dual specificity phosphatase Cdc25B
Gene namesi
Name:Cdc25b
Synonyms:Cdc25m2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:99701. Cdc25b.

Subcellular locationi

GO - Cellular componenti

  1. centrosome Source: UniProtKB
  2. cytoplasm Source: UniProtKB
  3. nucleus Source: UniProtKB
  4. spindle pole Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 576576M-phase inducer phosphatase 2PRO_0000198645Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei167 – 1671Phosphoserine; by MELKBy similarity
Modified residuei248 – 2481PhosphoserineBy similarity
Modified residuei321 – 3211Phosphoserine; by MELK and MAPK14By similarity
Modified residuei351 – 3511Phosphoserine; by AURKABy similarity
Modified residuei372 – 3721Phosphoserine; by BRSK1 and MAPK14By similarity

Post-translational modificationi

Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-351 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-167 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-321 and Ser-372 by MAPK14 is required for binding to 14-3-3 proteins (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP30306.

PTM databases

PhosphoSiteiP30306.

Expressioni

Tissue specificityi

Expressed predominantly in spleen, lung, heart, brain, intestine, and muscle.

Developmental stagei

Detected at the one-cell stage followed by a decrease in signal intensity at the two-cell stage. Detectable at higher level in the four-cell stage and expressed through the eight-cell, 16-cell and morula stages. Maximal expression at the blastocyst stage.

Gene expression databases

BgeeiP30306.
CleanExiMM_CDC25B.
ExpressionAtlasiP30306. baseline and differential.
GenevestigatoriP30306.

Interactioni

Subunit structurei

Interacts with MAPK14 and 14-3-3 proteins.By similarity

Protein-protein interaction databases

BioGridi198622. 2 interactions.
IntActiP30306. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliP30306.
SMRiP30306. Positions 383-560.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini427 – 534108RhodanesePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the MPI phosphatase family.Curated
Contains 1 rhodanese domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5105.
HOGENOMiHOG000082672.
HOVERGENiHBG052501.
InParanoidiP30306.
KOiK05866.
OMAiTPKSQVG.
OrthoDBiEOG7288R1.
PhylomeDBiP30306.
TreeFamiTF101056.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiIPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view]
PfamiPF06617. M-inducer_phosp. 1 hit.
PF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSiPR00716. MPIPHPHTASE.
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30306-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEVPLQKSAP GSALSPARVL GGIQRPRHLS VFEFESDGFL GSPEPTASSS
60 70 80 90 100
PVTTLTQTMH NLAGLGSEPP KAQVGSLSFQ NRLADLSLSR RTSECSLSSE
110 120 130 140 150
SSESSDAGLC MDSPSPVDPQ MAERTFEQAI QAASRVIQNE QFTIKRFRSL
160 170 180 190 200
PVRLLEHSPV LQSITNSRAL DSWRKTEAGY RAAANSPGED KENDGYIFKM
210 220 230 240 250
PQELPHSSSA QALAEWVSRR QAFTQRPSSA PDLMCLTTEW KMEVEELSPV
260 270 280 290 300
AQSSSLTPVE RASEEDDGFV DILESDLKDD EKVPAGMENL ISAPLVKKLD
310 320 330 340 350
KEEEQDLIMF SKCQRLFRSP SMPCSVIRPI LKRLERPQDR DVPVQSKRRK
360 370 380 390 400
SVTPLEEQQL EEPKARVFRS KSLCHEIENI LDSDHRGLIG DYSKAFLLQT
410 420 430 440 450
VDGKHQDLKY ISPETMVALL TGKFSNIVEK FVIVDCRYPY EYEGGHIKNA
460 470 480 490 500
VNLPLERDAE TFLLQRPIMP CSLDKRIILI FHCEFSSERG PRMCRFIRER
510 520 530 540 550
DRAANDYPSL YYPEMYILKG GYKEFFPQHP NFCEPQDYRP MNHEAFRDEL
560 570
RNFRLKTRSW AGERSRRELC SRLQDQ
Length:576
Mass (Da):65,490
Last modified:April 1, 1993 - v1
Checksum:i5086B676581CD570
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti532 – 5321F → L in AAH02287. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S93521 mRNA. Translation: AAB22026.1.
BC002287 mRNA. Translation: AAH02287.1.
CCDSiCCDS16758.1.
PIRiA42236.
RefSeqiNP_075606.1. NM_023117.4.
UniGeneiMm.38444.

Genome annotation databases

EnsembliENSMUST00000028804; ENSMUSP00000028804; ENSMUSG00000027330.
GeneIDi12531.
KEGGimmu:12531.
UCSCiuc008mky.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S93521 mRNA. Translation: AAB22026.1 .
BC002287 mRNA. Translation: AAH02287.1 .
CCDSi CCDS16758.1.
PIRi A42236.
RefSeqi NP_075606.1. NM_023117.4.
UniGenei Mm.38444.

3D structure databases

ProteinModelPortali P30306.
SMRi P30306. Positions 383-560.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 198622. 2 interactions.
IntActi P30306. 3 interactions.

Chemistry

BindingDBi P30306.
ChEMBLi CHEMBL2723.

PTM databases

PhosphoSitei P30306.

Proteomic databases

PRIDEi P30306.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000028804 ; ENSMUSP00000028804 ; ENSMUSG00000027330 .
GeneIDi 12531.
KEGGi mmu:12531.
UCSCi uc008mky.3. mouse.

Organism-specific databases

CTDi 994.
MGIi MGI:99701. Cdc25b.

Phylogenomic databases

eggNOGi COG5105.
HOGENOMi HOG000082672.
HOVERGENi HBG052501.
InParanoidi P30306.
KOi K05866.
OMAi TPKSQVG.
OrthoDBi EOG7288R1.
PhylomeDBi P30306.
TreeFami TF101056.

Enzyme and pathway databases

Reactomei REACT_206803. Cyclin A/B1 associated events during G2/M transition.
REACT_209377. Cyclin B2 mediated events.

Miscellaneous databases

NextBioi 281562.
PROi P30306.
SOURCEi Search...

Gene expression databases

Bgeei P30306.
CleanExi MM_CDC25B.
ExpressionAtlasi P30306. baseline and differential.
Genevestigatori P30306.

Family and domain databases

Gene3Di 3.40.250.10. 1 hit.
InterProi IPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view ]
Pfami PF06617. M-inducer_phosp. 1 hit.
PF00581. Rhodanese. 1 hit.
[Graphical view ]
PRINTSi PR00716. MPIPHPHTASE.
SMARTi SM00450. RHOD. 1 hit.
[Graphical view ]
SUPFAMi SSF52821. SSF52821. 1 hit.
PROSITEi PS50206. RHODANESE_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 328-576.

Entry informationi

Entry nameiMPIP2_MOUSE
AccessioniPrimary (citable) accession number: P30306
Secondary accession number(s): Q99LP3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: October 29, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3