Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P30306

- MPIP2_MOUSE

UniProt

P30306 - MPIP2_MOUSE

Protein

M-phase inducer phosphatase 2

Gene

Cdc25b

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity By similarity.By similarity

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

    Enzyme regulationi

    Stimulated by B-type cyclins.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei483 – 4831By similarity

    GO - Molecular functioni

    1. protein tyrosine phosphatase activity Source: UniProtKB-EC

    GO - Biological processi

    1. female meiosis I Source: MGI
    2. mitotic nuclear division Source: UniProtKB-KW
    3. oocyte maturation Source: MGI
    4. positive regulation of cytokinesis Source: Ensembl
    5. positive regulation of mitotic cell cycle Source: Ensembl
    6. positive regulation of protein kinase activity Source: MGI
    7. protein phosphorylation Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Enzyme and pathway databases

    ReactomeiREACT_206803. Cyclin A/B1 associated events during G2/M transition.
    REACT_209377. Cyclin B2 mediated events.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    M-phase inducer phosphatase 2 (EC:3.1.3.48)
    Alternative name(s):
    Dual specificity phosphatase Cdc25B
    Gene namesi
    Name:Cdc25b
    Synonyms:Cdc25m2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:99701. Cdc25b.

    Subcellular locationi

    GO - Cellular componenti

    1. centrosome Source: UniProtKB
    2. cytoplasm Source: UniProtKB
    3. nucleus Source: UniProtKB
    4. spindle pole Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 576576M-phase inducer phosphatase 2PRO_0000198645Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei167 – 1671Phosphoserine; by MELKBy similarity
    Modified residuei248 – 2481PhosphoserineBy similarity
    Modified residuei321 – 3211Phosphoserine; by MELK and MAPK14By similarity
    Modified residuei351 – 3511Phosphoserine; by AURKABy similarity
    Modified residuei372 – 3721Phosphoserine; by BRSK1 and MAPK14By similarity

    Post-translational modificationi

    Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-351 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-167 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-321 and Ser-372 by MAPK14 is required for binding to 14-3-3 proteins By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiP30306.

    PTM databases

    PhosphoSiteiP30306.

    Expressioni

    Tissue specificityi

    Expressed predominantly in spleen, lung, heart, brain, intestine, and muscle.

    Developmental stagei

    Detected at the one-cell stage followed by a decrease in signal intensity at the two-cell stage. Detectable at higher level in the four-cell stage and expressed through the eight-cell, 16-cell and morula stages. Maximal expression at the blastocyst stage.

    Gene expression databases

    ArrayExpressiP30306.
    BgeeiP30306.
    CleanExiMM_CDC25B.
    GenevestigatoriP30306.

    Interactioni

    Subunit structurei

    Interacts with MAPK14 and 14-3-3 proteins.By similarity

    Protein-protein interaction databases

    BioGridi198622. 2 interactions.
    IntActiP30306. 3 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP30306.
    SMRiP30306. Positions 383-560.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini427 – 534108RhodanesePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the MPI phosphatase family.Curated
    Contains 1 rhodanese domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5105.
    HOGENOMiHOG000082672.
    HOVERGENiHBG052501.
    InParanoidiP30306.
    KOiK05866.
    OMAiTPKSQVG.
    OrthoDBiEOG7288R1.
    PhylomeDBiP30306.
    TreeFamiTF101056.

    Family and domain databases

    Gene3Di3.40.250.10. 1 hit.
    InterProiIPR000751. MPI_Phosphatase.
    IPR001763. Rhodanese-like_dom.
    [Graphical view]
    PfamiPF06617. M-inducer_phosp. 1 hit.
    PF00581. Rhodanese. 1 hit.
    [Graphical view]
    PRINTSiPR00716. MPIPHPHTASE.
    SMARTiSM00450. RHOD. 1 hit.
    [Graphical view]
    SUPFAMiSSF52821. SSF52821. 1 hit.
    PROSITEiPS50206. RHODANESE_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P30306-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEVPLQKSAP GSALSPARVL GGIQRPRHLS VFEFESDGFL GSPEPTASSS    50
    PVTTLTQTMH NLAGLGSEPP KAQVGSLSFQ NRLADLSLSR RTSECSLSSE 100
    SSESSDAGLC MDSPSPVDPQ MAERTFEQAI QAASRVIQNE QFTIKRFRSL 150
    PVRLLEHSPV LQSITNSRAL DSWRKTEAGY RAAANSPGED KENDGYIFKM 200
    PQELPHSSSA QALAEWVSRR QAFTQRPSSA PDLMCLTTEW KMEVEELSPV 250
    AQSSSLTPVE RASEEDDGFV DILESDLKDD EKVPAGMENL ISAPLVKKLD 300
    KEEEQDLIMF SKCQRLFRSP SMPCSVIRPI LKRLERPQDR DVPVQSKRRK 350
    SVTPLEEQQL EEPKARVFRS KSLCHEIENI LDSDHRGLIG DYSKAFLLQT 400
    VDGKHQDLKY ISPETMVALL TGKFSNIVEK FVIVDCRYPY EYEGGHIKNA 450
    VNLPLERDAE TFLLQRPIMP CSLDKRIILI FHCEFSSERG PRMCRFIRER 500
    DRAANDYPSL YYPEMYILKG GYKEFFPQHP NFCEPQDYRP MNHEAFRDEL 550
    RNFRLKTRSW AGERSRRELC SRLQDQ 576
    Length:576
    Mass (Da):65,490
    Last modified:April 1, 1993 - v1
    Checksum:i5086B676581CD570
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti532 – 5321F → L in AAH02287. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S93521 mRNA. Translation: AAB22026.1.
    BC002287 mRNA. Translation: AAH02287.1.
    CCDSiCCDS16758.1.
    PIRiA42236.
    RefSeqiNP_075606.1. NM_023117.4.
    UniGeneiMm.38444.

    Genome annotation databases

    EnsembliENSMUST00000028804; ENSMUSP00000028804; ENSMUSG00000027330.
    GeneIDi12531.
    KEGGimmu:12531.
    UCSCiuc008mky.3. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S93521 mRNA. Translation: AAB22026.1 .
    BC002287 mRNA. Translation: AAH02287.1 .
    CCDSi CCDS16758.1.
    PIRi A42236.
    RefSeqi NP_075606.1. NM_023117.4.
    UniGenei Mm.38444.

    3D structure databases

    ProteinModelPortali P30306.
    SMRi P30306. Positions 383-560.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198622. 2 interactions.
    IntActi P30306. 3 interactions.

    Chemistry

    BindingDBi P30306.
    ChEMBLi CHEMBL2723.

    PTM databases

    PhosphoSitei P30306.

    Proteomic databases

    PRIDEi P30306.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000028804 ; ENSMUSP00000028804 ; ENSMUSG00000027330 .
    GeneIDi 12531.
    KEGGi mmu:12531.
    UCSCi uc008mky.3. mouse.

    Organism-specific databases

    CTDi 994.
    MGIi MGI:99701. Cdc25b.

    Phylogenomic databases

    eggNOGi COG5105.
    HOGENOMi HOG000082672.
    HOVERGENi HBG052501.
    InParanoidi P30306.
    KOi K05866.
    OMAi TPKSQVG.
    OrthoDBi EOG7288R1.
    PhylomeDBi P30306.
    TreeFami TF101056.

    Enzyme and pathway databases

    Reactomei REACT_206803. Cyclin A/B1 associated events during G2/M transition.
    REACT_209377. Cyclin B2 mediated events.

    Miscellaneous databases

    NextBioi 281562.
    PROi P30306.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P30306.
    Bgeei P30306.
    CleanExi MM_CDC25B.
    Genevestigatori P30306.

    Family and domain databases

    Gene3Di 3.40.250.10. 1 hit.
    InterProi IPR000751. MPI_Phosphatase.
    IPR001763. Rhodanese-like_dom.
    [Graphical view ]
    Pfami PF06617. M-inducer_phosp. 1 hit.
    PF00581. Rhodanese. 1 hit.
    [Graphical view ]
    PRINTSi PR00716. MPIPHPHTASE.
    SMARTi SM00450. RHOD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52821. SSF52821. 1 hit.
    PROSITEi PS50206. RHODANESE_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 328-576.

    Entry informationi

    Entry nameiMPIP2_MOUSE
    AccessioniPrimary (citable) accession number: P30306
    Secondary accession number(s): Q99LP3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3