M-phase inducer phosphatase 2 - Mus musculus (Mouse)

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P30306 (MPIP2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 109. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
M-phase inducer phosphatase 2
EC=3.1.3.48

Alternative name(s):
Dual specificity phosphatase Cdc25B

Gene names

Name:	Cdc25b
Synonyms:	Cdc25m2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	576 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity By similarity.
Catalytic activity	Protein tyrosine phosphate + H₂O = protein tyrosine + phosphate.
Enzyme regulation	Stimulated by B-type cyclins.
Subunit structure	Interacts with MAPK14 and 14-3-3 proteins By similarity.
Subcellular location	Cytoplasm › cytoskeleton › centrosome By similarity. Cytoplasm › cytoskeleton › spindle pole By similarity.
Tissue specificity	Expressed predominantly in spleen, lung, heart, brain, intestine, and muscle.
Developmental stage	Detected at the one-cell stage followed by a decrease in signal intensity at the two-cell stage. Detectable at higher level in the four-cell stage and expressed through the eight-cell, 16-cell and morula stages. Maximal expression at the blastocyst stage.
Post-translational modification	Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-351 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-167 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-321 and Ser-372 by MAPK14 is required for binding to 14-3-3 proteins By similarity.
Sequence similarities	Belongs to the MPI phosphatase family. Contains 1 rhodanese domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm Cytoskeleton
Molecular function	Hydrolase Protein phosphatase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW female meiosis I Inferred from mutant phenotype PubMed 11912493. Source: MGI mitosis Inferred from electronic annotation. Source: UniProtKB-KW oocyte maturation Inferred from mutant phenotype PubMed 11912493. Source: MGI peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC positive regulation of cell cycle cytokinesis Inferred from electronic annotation. Source: Compara positive regulation of mitotic cell cycle Inferred from electronic annotation. Source: Compara positive regulation of protein kinase activity Inferred from mutant phenotype PubMed 11912493. Source: MGI protein phosphorylation Inferred from electronic annotation. Source: Compara
Cellular_component	centrosome Inferred from sequence or structural similarity. Source: UniProtKB cytoplasm Inferred from direct assay PubMed 20083600. Source: UniProtKB nucleus Inferred from direct assay PubMed 20083600. Source: UniProtKB spindle pole Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 576

576

M-phase inducer phosphatase 2

PRO_0000198645

Regions

Domain

427 – 534

108

Rhodanese

Sites

Active site

483

By similarity

Amino acid modifications

Modified residue

167

Phosphoserine; by MELK By similarity

Modified residue

248

Phosphoserine By similarity

Modified residue

321

Phosphoserine; by MELK and MAPK14 By similarity

Modified residue

351

Phosphoserine; by AURKA By similarity

Modified residue

372

Phosphoserine; by BRSK1 and MAPK14 By similarity

Experimental info

Sequence conflict

532

F → L in AAH02287. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P30306 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 5086B676581CD570
Show »

FASTA

576

65,490

        10         20         30         40         50         60 
MEVPLQKSAP GSALSPARVL GGIQRPRHLS VFEFESDGFL GSPEPTASSS PVTTLTQTMH 

        70         80         90        100        110        120 
NLAGLGSEPP KAQVGSLSFQ NRLADLSLSR RTSECSLSSE SSESSDAGLC MDSPSPVDPQ 

       130        140        150        160        170        180 
MAERTFEQAI QAASRVIQNE QFTIKRFRSL PVRLLEHSPV LQSITNSRAL DSWRKTEAGY 

       190        200        210        220        230        240 
RAAANSPGED KENDGYIFKM PQELPHSSSA QALAEWVSRR QAFTQRPSSA PDLMCLTTEW 

       250        260        270        280        290        300 
KMEVEELSPV AQSSSLTPVE RASEEDDGFV DILESDLKDD EKVPAGMENL ISAPLVKKLD 

       310        320        330        340        350        360 
KEEEQDLIMF SKCQRLFRSP SMPCSVIRPI LKRLERPQDR DVPVQSKRRK SVTPLEEQQL 

       370        380        390        400        410        420 
EEPKARVFRS KSLCHEIENI LDSDHRGLIG DYSKAFLLQT VDGKHQDLKY ISPETMVALL 

       430        440        450        460        470        480 
TGKFSNIVEK FVIVDCRYPY EYEGGHIKNA VNLPLERDAE TFLLQRPIMP CSLDKRIILI 

       490        500        510        520        530        540 
FHCEFSSERG PRMCRFIRER DRAANDYPSL YYPEMYILKG GYKEFFPQHP NFCEPQDYRP 

       550        560        570 
MNHEAFRDEL RNFRLKTRSW AGERSRRELC SRLQDQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A mouse cdc25 homolog is differentially and developmentally expressed." Kakizuka A., Sebastian B., Borgmeyer U., Hermans-Borgmeyer I., Bolado J., Hunter T., Hoekstra M.F., Evans R.M. Genes Dev. 6:578-590(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 328-576.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S93521 mRNA. Translation: AAB22026.1. BC002287 mRNA. Translation: AAH02287.1.
IPI	IPI00129197.
PIR	A42236.
RefSeq	NP_075606.1. NM_023117.4.
UniGene	Mm.38444.
3D structure databases
ProteinModelPortal	P30306.
SMR	P30306. Positions 383-560.
ModBase	Search...
Protein-protein interaction databases
IntAct	P30306. 1 interaction.
PTM databases
PhosphoSite	P30306.
Proteomic databases
PRIDE	P30306.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000028804; ENSMUSP00000028804; ENSMUSG00000027330.
GeneID	12531.
KEGG	mmu:12531.
Organism-specific databases
CTD	994.
MGI	MGI:99701. Cdc25b.
Phylogenomic databases
eggNOG	COG5105.
HOGENOM	HOG000082672.
HOVERGEN	HBG052501.
InParanoid	P30306.
KO	K05866.
OMA	THALAEW.
OrthoDB	EOG408N87.
Gene expression databases
ArrayExpress	P30306.
Bgee	P30306.
CleanEx	MM_CDC25B.
Genevestigator	P30306.
GermOnline	ENSMUSG00000027330. Mus musculus.
Family and domain databases
Gene3D	3.40.250.10. 1 hit.
InterPro	IPR000751. MPI_Phosphatase. IPR001763. Rhodanese-like_dom. [Graphical view]
PANTHER	PTHR10828. PTHR10828. 1 hit.
Pfam	PF06617. M-inducer_phosp. 1 hit. PF00581. Rhodanese. 1 hit. [Graphical view]
PRINTS	PR00716. MPIPHPHTASE.
SMART	SM00450. RHOD. 1 hit. [Graphical view]
SUPFAM	SSF52821. Rhodanese-like. 1 hit.
PROSITE	PS50206. RHODANESE_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P30306.
ChEMBL	CHEMBL2723.
NextBio	281562.
SOURCE	Search...

Entry information

Entry name

MPIP2_MOUSE

Accession

Primary (citable) accession number: P30306
Secondary accession number(s): Q99LP3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	April 3, 2013
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents