P30303 (MPIP_EMENI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
M-phase inducer phosphatase
EC=3.1.3.48

Gene names

Name:	nimT
ORF Names:	AN3941

Organism

Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) [Reference proteome]

Taxonomic identifier

227321 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Emericella › Emericella nidulans › mitosporic Emericella nidulans

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This protein functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It may directly dephosphorylate p34(cdc2) and activate the p34(cdc2) kinase activity.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Sequence similarities	Belongs to the MPI phosphatase family. Contains 1 rhodanese domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	M phase of mitotic cell cycle Inferred from electronic annotation. Source: InterPro cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 556	556	M-phase inducer phosphatase		PRO_0000198660
Regions
	Domain	371 – 474	104	Rhodanese
Sites
	Active site	421	1	By similarity
Experimental info
	Sequence conflict	353	1	P → G in CAA45885. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P30303 [UniParc]. Last modified May 1, 2007. Version 2. Checksum: F558E77944A0BA59	FASTA	556	61,626
	10 20 30 40 50 60 MEHSSPLAAM QPPSVMLGHC FRSDAPTSYH GFSPLPGLGP GGFNFKDLSM KRSNGDYFGT 70 80 90 100 110 120 KVVRGSSPTA SLAADLSQNF HIDQSPQVAT PRRSLFSACL LGNGNRRGVD DAMTTPPLPS 130 140 150 160 170 180 SSPAPAMDIM DMSPLPHKPP FISTPEIELD SPTLESSPMD TTMMSTDGLV PDSPTVLPKD 190 200 210 220 230 240 GKQERRRPTF LRPSLARSKA QSFQVGMTRP APESQGPPFK FQTNGINKTS SGVAASLEDM 250 260 270 280 290 300 FGESPQRERP MMRINSTSGL NSRLRPPLGS GSHVRGNGSP SAASVRKSAH PNMRPRKQCR 310 320 330 340 350 360 RSLSMYEHPE DVIADSEVSY TSNAPLQSIS DFEETQALQL PHFIPEEQAD NLPRIDKATL 370 380 390 400 410 420 VDIKEGKYDN MFDNIMIIDC RFEYEYDGGH IVGAVNYNDK ENLAAELFAD PKPRTAIVFH 430 440 450 460 470 480 CEYSVHRAPL MAKYIRHRDR AYNVDHYPQL SYPDMYILEG GYSGFFAEHR SLCYPQNYVE 490 500 510 520 530 540 MSAKEHEFAC ERGLGKVKQR SKLSRAQTFA FGQQSPEMED SPTGRCRNNP GDRKLLASPF 550 NDSPGSRFPG RRMLSY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An extra copy of nimEcyclinB elevates pre-MPF levels and partially suppresses mutation of nimTcdc25 in Aspergillus nidulans." O'Connell M.J., Osmani A.H., Morris N.R., Osmani S.A. EMBO J. 11:2139-2149(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: GB20.
[2]	"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae." Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. , Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W. Nature 438:1105-1115(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
[3]	"The 2008 update of the Aspergillus nidulans genome annotation: a community effort." Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G. , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G. Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X64601 mRNA. Translation: CAA45885.1. AACD01000064 Genomic DNA. Translation: EAA59250.1. BN001302 Genomic DNA. Translation: CBF75046.1.
PIR	S24395.
RefSeq	XP_661545.1. XM_656453.1.
3D structure databases
ProteinModelPortal	P30303.
ModBase	Search...
Protein-protein interaction databases
STRING	162425.CADANIAP00004753.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADANIAT00004753; CADANIAP00004753; CADANIAG00004753.
GeneID	2873361.
KEGG	ani:AN3941.2.
Phylogenomic databases
eggNOG	COG5105.
HOGENOM	HOG000215320.
KO	K02555.
OMA	EYSAHRA.
OrthoDB	EOG4VQDZR.
Family and domain databases
Gene3D	3.40.250.10. 1 hit.
InterPro	IPR000751. MPI_Phosphatase. IPR001763. Rhodanese-like_dom. [Graphical view]
PANTHER	PTHR10828. PTHR10828. 1 hit.
Pfam	PF00581. Rhodanese. 1 hit. [Graphical view]
PRINTS	PR00716. MPIPHPHTASE.
SMART	SM00450. RHOD. 1 hit. [Graphical view]
SUPFAM	SSF52821. Rhodanese-like. 1 hit.
PROSITE	PS50206. RHODANESE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MPIP_EMENI

Accession

Primary (citable) accession number: P30303
Secondary accession number(s): C8V635, Q5B689

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	May 1, 2007
Last modified:	May 29, 2013
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program