Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P30290 (MIK1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitosis inhibitor protein kinase mik1

EC=2.7.11.1
Gene names
Name:mik1
ORF Names:SPBC660.14
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces

Protein attributes

Sequence length581 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Protein kinase that acts both on serines and on tyrosines. It acts as a negative regulator of entry into mitosis (G2 to M transition). Phosphorylates and inhibits cdc2.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 581581Mitosis inhibitor protein kinase mik1
PRO_0000086327

Regions

Domain289 – 561273Protein kinase
Nucleotide binding295 – 3039ATP By similarity

Sites

Active site4171Proton acceptor By similarity
Metal binding4221Magnesium; via carbonyl oxygen By similarity
Metal binding4351Magnesium; via carbonyl oxygen By similarity
Binding site3201ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P30290 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 371E0CEDB2CA1CE3

FASTA58165,934
        10         20         30         40         50         60 
MDSSTTIPIT PTRTPCFFNI SSSFNEHSPL NFYDEPIYNF SSGHEENQSH KSSKLTFFKP 

        70         80         90        100        110        120 
SNTKRSPHTP MQNNAKAIRL STTVRHGIFK NSDLDGCSKP FAFSSGLKLS KKIVDASTPI 

       130        140        150        160        170        180 
DLKRKRAVTS LSTGLLSKRE KWSLWEGNLT NPRSEQPHTP CKKGTKIKLK PPQSPLSPTT 

       190        200        210        220        230        240 
SLLARKCKHI DLDTFSRLDH PNSDSSDETF EMEELPSLSY GSEDLLEFCE TPCKSQPIFL 

       250        260        270        280        290        300 
SSSHVNNWDE KDVPSSLSWT PTSPIFLNIN SADDYEEEED WTSDLRIRFQ QVKPIHESDF 

       310        320        330        340        350        360 
SFVYHVSSIN PPTETVYVVK MLKKNAAKFT GKERHLQEVS ILQRLQACPF VVNLVNVWSY 

       370        380        390        400        410        420 
NDNIFLQLDY CENGDLSLFL SELGLLQVMD PFRVWKMLFQ LTQALNFIHL LEFVHLDVKP 

       430        440        450        460        470        480 
SNVLITRDGN LKLGDFGLAT SLPVSSMVDL EGDRVYIAPE ILASHNYGKP ADVYSLGLSM 

       490        500        510        520        530        540 
IEAATNVVLP ENGVEWQRLR SGDYSNLPNL KDLLLSKEKV QINKVRCAES LQCLLQRMTH 

       550        560        570        580 
PYVDCRPTTQ DLLAMPEMIF ISEHSQKAAI IYEDHNSWLE T 

« Hide

References

« Hide 'large scale' references
[1]"mik1 and wee1 cooperate in the inhibitory tyrosine phosphorylation of cdc2."
Lundgren K., Walworth N., Booher R., Dembski M., Kirschner M., Beach D.
Cell 64:1111-1122(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M60834 Genomic DNA. Translation: AAA91278.1.
CU329671 Genomic DNA. Translation: CAA22534.1.
PIRA37913.
RefSeqNP_595093.1. NM_001021000.2.

3D structure databases

ProteinModelPortalP30290.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid277402. 54 interactions.
MINTMINT-4688175.
STRING4896.SPBC660.14-1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPBC660.14.1; SPBC660.14.1:pep; SPBC660.14.
GeneID2540885.
KEGGspo:SPBC660.14.

Organism-specific databases

PomBaseSPBC660.14.

Phylogenomic databases

eggNOGCOG0515.
OrthoDBEOG7380FJ.
PhylomeDBP30290.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20802001.

Entry information

Entry nameMIK1_SCHPO
AccessionPrimary (citable) accession number: P30290
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: April 16, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names