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Protein

Mitosis inhibitor protein kinase mik1

Gene

mik1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Protein kinase that acts both on serines and on tyrosines. It acts as a negative regulator of entry into mitosis (G2 to M transition). Phosphorylates and inhibits cdc2.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei320 – 3201ATPPROSITE-ProRule annotation
Active sitei417 – 4171Proton acceptorPROSITE-ProRule annotation
Metal bindingi422 – 4221Magnesium; via carbonyl oxygenBy similarity
Metal bindingi435 – 4351Magnesium; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi295 – 3039ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • mitotic nuclear division Source: UniProtKB-KW
  • negative regulation of G2/M transition of mitotic cell cycle Source: PomBase
  • peptidyl-tyrosine phosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_291429. Cyclin A/B1 associated events during G2/M transition.
REACT_297137. G2/M DNA replication checkpoint.
REACT_302677. Polo-like kinase mediated events.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitosis inhibitor protein kinase mik1 (EC:2.7.11.1)
Gene namesi
Name:mik1
ORF Names:SPBC660.14
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC660.14.
PomBaseiSPBC660.14. mik1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 581581Mitosis inhibitor protein kinase mik1PRO_0000086327Add
BLAST

Interactioni

Protein-protein interaction databases

BioGridi277402. 57 interactions.
MINTiMINT-4688175.
STRINGi4896.SPBC660.14.1.

Structurei

3D structure databases

ProteinModelPortaliP30290.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini289 – 561273Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
InParanoidiP30290.
OMAiFLNINSA.
OrthoDBiEOG7380FJ.
PhylomeDBiP30290.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30290-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSSTTIPIT PTRTPCFFNI SSSFNEHSPL NFYDEPIYNF SSGHEENQSH
60 70 80 90 100
KSSKLTFFKP SNTKRSPHTP MQNNAKAIRL STTVRHGIFK NSDLDGCSKP
110 120 130 140 150
FAFSSGLKLS KKIVDASTPI DLKRKRAVTS LSTGLLSKRE KWSLWEGNLT
160 170 180 190 200
NPRSEQPHTP CKKGTKIKLK PPQSPLSPTT SLLARKCKHI DLDTFSRLDH
210 220 230 240 250
PNSDSSDETF EMEELPSLSY GSEDLLEFCE TPCKSQPIFL SSSHVNNWDE
260 270 280 290 300
KDVPSSLSWT PTSPIFLNIN SADDYEEEED WTSDLRIRFQ QVKPIHESDF
310 320 330 340 350
SFVYHVSSIN PPTETVYVVK MLKKNAAKFT GKERHLQEVS ILQRLQACPF
360 370 380 390 400
VVNLVNVWSY NDNIFLQLDY CENGDLSLFL SELGLLQVMD PFRVWKMLFQ
410 420 430 440 450
LTQALNFIHL LEFVHLDVKP SNVLITRDGN LKLGDFGLAT SLPVSSMVDL
460 470 480 490 500
EGDRVYIAPE ILASHNYGKP ADVYSLGLSM IEAATNVVLP ENGVEWQRLR
510 520 530 540 550
SGDYSNLPNL KDLLLSKEKV QINKVRCAES LQCLLQRMTH PYVDCRPTTQ
560 570 580
DLLAMPEMIF ISEHSQKAAI IYEDHNSWLE T
Length:581
Mass (Da):65,934
Last modified:April 1, 1993 - v1
Checksum:i371E0CEDB2CA1CE3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60834 Genomic DNA. Translation: AAA91278.1.
CU329671 Genomic DNA. Translation: CAA22534.1.
PIRiA37913.
RefSeqiNP_595093.1. NM_001021000.2.

Genome annotation databases

EnsemblFungiiSPBC660.14.1; SPBC660.14.1:pep; SPBC660.14.
GeneIDi2540885.
KEGGispo:SPBC660.14.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60834 Genomic DNA. Translation: AAA91278.1.
CU329671 Genomic DNA. Translation: CAA22534.1.
PIRiA37913.
RefSeqiNP_595093.1. NM_001021000.2.

3D structure databases

ProteinModelPortaliP30290.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277402. 57 interactions.
MINTiMINT-4688175.
STRINGi4896.SPBC660.14.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC660.14.1; SPBC660.14.1:pep; SPBC660.14.
GeneIDi2540885.
KEGGispo:SPBC660.14.

Organism-specific databases

EuPathDBiFungiDB:SPBC660.14.
PomBaseiSPBC660.14. mik1.

Phylogenomic databases

eggNOGiCOG0515.
InParanoidiP30290.
OMAiFLNINSA.
OrthoDBiEOG7380FJ.
PhylomeDBiP30290.

Enzyme and pathway databases

ReactomeiREACT_291429. Cyclin A/B1 associated events during G2/M transition.
REACT_297137. G2/M DNA replication checkpoint.
REACT_302677. Polo-like kinase mediated events.

Miscellaneous databases

NextBioi20802001.
PROiP30290.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "mik1 and wee1 cooperate in the inhibitory tyrosine phosphorylation of cdc2."
    Lundgren K., Walworth N., Booher R., Dembski M., Kirschner M., Beach D.
    Cell 64:1111-1122(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiMIK1_SCHPO
AccessioniPrimary (citable) accession number: P30290
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: June 24, 2015
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.