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Protein

Mitosis inhibitor protein kinase mik1

Gene

mik1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Protein kinase that acts both on serines and on tyrosines. It acts as a negative regulator of entry into mitosis (G2 to M transition). Phosphorylates and inhibits cdc2.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei320ATPPROSITE-ProRule annotation1
Active sitei417Proton acceptorPROSITE-ProRule annotation1
Metal bindingi422Magnesium; via carbonyl oxygenBy similarity1
Metal bindingi435Magnesium; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi295 – 303ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • mitotic DNA replication checkpoint Source: PomBase
  • negative regulation of G2/M transition of mitotic cell cycle Source: PomBase
  • negative regulation of meiotic nuclear division Source: PomBase

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase
Biological processCell cycle, Cell division, Mitosis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-156711. Polo-like kinase mediated events.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitosis inhibitor protein kinase mik1 (EC:2.7.11.1)
Gene namesi
Name:mik1
ORF Names:SPBC660.14
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC660.14.
PomBaseiSPBC660.14. mik1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000863271 – 581Mitosis inhibitor protein kinase mik1Add BLAST581

Proteomic databases

PRIDEiP30290.

Interactioni

Protein-protein interaction databases

BioGridi277402. 60 interactors.
MINTiMINT-4688175.
STRINGi4896.SPBC660.14.1.

Structurei

3D structure databases

ProteinModelPortaliP30290.
SMRiP30290.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini289 – 561Protein kinasePROSITE-ProRule annotationAdd BLAST273

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.PROSITE-ProRule annotation

Phylogenomic databases

InParanoidiP30290.
OrthoDBiEOG092C38EF.
PhylomeDBiP30290.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

P30290-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSSTTIPIT PTRTPCFFNI SSSFNEHSPL NFYDEPIYNF SSGHEENQSH
60 70 80 90 100
KSSKLTFFKP SNTKRSPHTP MQNNAKAIRL STTVRHGIFK NSDLDGCSKP
110 120 130 140 150
FAFSSGLKLS KKIVDASTPI DLKRKRAVTS LSTGLLSKRE KWSLWEGNLT
160 170 180 190 200
NPRSEQPHTP CKKGTKIKLK PPQSPLSPTT SLLARKCKHI DLDTFSRLDH
210 220 230 240 250
PNSDSSDETF EMEELPSLSY GSEDLLEFCE TPCKSQPIFL SSSHVNNWDE
260 270 280 290 300
KDVPSSLSWT PTSPIFLNIN SADDYEEEED WTSDLRIRFQ QVKPIHESDF
310 320 330 340 350
SFVYHVSSIN PPTETVYVVK MLKKNAAKFT GKERHLQEVS ILQRLQACPF
360 370 380 390 400
VVNLVNVWSY NDNIFLQLDY CENGDLSLFL SELGLLQVMD PFRVWKMLFQ
410 420 430 440 450
LTQALNFIHL LEFVHLDVKP SNVLITRDGN LKLGDFGLAT SLPVSSMVDL
460 470 480 490 500
EGDRVYIAPE ILASHNYGKP ADVYSLGLSM IEAATNVVLP ENGVEWQRLR
510 520 530 540 550
SGDYSNLPNL KDLLLSKEKV QINKVRCAES LQCLLQRMTH PYVDCRPTTQ
560 570 580
DLLAMPEMIF ISEHSQKAAI IYEDHNSWLE T
Length:581
Mass (Da):65,934
Last modified:April 1, 1993 - v1
Checksum:i371E0CEDB2CA1CE3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60834 Genomic DNA. Translation: AAA91278.1.
CU329671 Genomic DNA. Translation: CAA22534.1.
PIRiA37913.
RefSeqiNP_595093.1. NM_001021000.2.

Genome annotation databases

EnsemblFungiiSPBC660.14.1; SPBC660.14.1:pep; SPBC660.14.
GeneIDi2540885.
KEGGispo:SPBC660.14.

Entry informationi

Entry nameiMIK1_SCHPO
AccessioniPrimary (citable) accession number: P30290
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: September 27, 2017
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families