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P30290

- MIK1_SCHPO

UniProt

P30290 - MIK1_SCHPO

Protein

Mitosis inhibitor protein kinase mik1

Gene

mik1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Protein kinase that acts both on serines and on tyrosines. It acts as a negative regulator of entry into mitosis (G2 to M transition). Phosphorylates and inhibits cdc2.

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei320 – 3201ATPPROSITE-ProRule annotation
    Active sitei417 – 4171Proton acceptorPROSITE-ProRule annotation
    Metal bindingi422 – 4221Magnesium; via carbonyl oxygenBy similarity
    Metal bindingi435 – 4351Magnesium; via carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi295 – 3039ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. protein serine/threonine kinase activity Source: PomBase
    4. protein tyrosine kinase activity Source: PomBase

    GO - Biological processi

    1. mitotic nuclear division Source: UniProtKB-KW
    2. negative regulation of G2/M transition of mitotic cell cycle Source: PomBase
    3. peptidyl-tyrosine phosphorylation Source: PomBase

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitosis inhibitor protein kinase mik1 (EC:2.7.11.1)
    Gene namesi
    Name:mik1
    ORF Names:SPBC660.14
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBC660.14.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: PomBase
    2. nucleus Source: PomBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 581581Mitosis inhibitor protein kinase mik1PRO_0000086327Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    BioGridi277402. 55 interactions.
    MINTiMINT-4688175.
    STRINGi4896.SPBC660.14-1.

    Structurei

    3D structure databases

    ProteinModelPortaliP30290.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini289 – 561273Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    OMAiWHITSKE.
    OrthoDBiEOG7380FJ.
    PhylomeDBiP30290.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P30290-1 [UniParc]FASTAAdd to Basket

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    MDSSTTIPIT PTRTPCFFNI SSSFNEHSPL NFYDEPIYNF SSGHEENQSH    50
    KSSKLTFFKP SNTKRSPHTP MQNNAKAIRL STTVRHGIFK NSDLDGCSKP 100
    FAFSSGLKLS KKIVDASTPI DLKRKRAVTS LSTGLLSKRE KWSLWEGNLT 150
    NPRSEQPHTP CKKGTKIKLK PPQSPLSPTT SLLARKCKHI DLDTFSRLDH 200
    PNSDSSDETF EMEELPSLSY GSEDLLEFCE TPCKSQPIFL SSSHVNNWDE 250
    KDVPSSLSWT PTSPIFLNIN SADDYEEEED WTSDLRIRFQ QVKPIHESDF 300
    SFVYHVSSIN PPTETVYVVK MLKKNAAKFT GKERHLQEVS ILQRLQACPF 350
    VVNLVNVWSY NDNIFLQLDY CENGDLSLFL SELGLLQVMD PFRVWKMLFQ 400
    LTQALNFIHL LEFVHLDVKP SNVLITRDGN LKLGDFGLAT SLPVSSMVDL 450
    EGDRVYIAPE ILASHNYGKP ADVYSLGLSM IEAATNVVLP ENGVEWQRLR 500
    SGDYSNLPNL KDLLLSKEKV QINKVRCAES LQCLLQRMTH PYVDCRPTTQ 550
    DLLAMPEMIF ISEHSQKAAI IYEDHNSWLE T 581
    Length:581
    Mass (Da):65,934
    Last modified:April 1, 1993 - v1
    Checksum:i371E0CEDB2CA1CE3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60834 Genomic DNA. Translation: AAA91278.1.
    CU329671 Genomic DNA. Translation: CAA22534.1.
    PIRiA37913.
    RefSeqiNP_595093.1. NM_001021000.2.

    Genome annotation databases

    EnsemblFungiiSPBC660.14.1; SPBC660.14.1:pep; SPBC660.14.
    GeneIDi2540885.
    KEGGispo:SPBC660.14.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60834 Genomic DNA. Translation: AAA91278.1 .
    CU329671 Genomic DNA. Translation: CAA22534.1 .
    PIRi A37913.
    RefSeqi NP_595093.1. NM_001021000.2.

    3D structure databases

    ProteinModelPortali P30290.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 277402. 55 interactions.
    MINTi MINT-4688175.
    STRINGi 4896.SPBC660.14-1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBC660.14.1 ; SPBC660.14.1:pep ; SPBC660.14 .
    GeneIDi 2540885.
    KEGGi spo:SPBC660.14.

    Organism-specific databases

    PomBasei SPBC660.14.

    Phylogenomic databases

    eggNOGi COG0515.
    OMAi WHITSKE.
    OrthoDBi EOG7380FJ.
    PhylomeDBi P30290.

    Miscellaneous databases

    NextBioi 20802001.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "mik1 and wee1 cooperate in the inhibitory tyrosine phosphorylation of cdc2."
      Lundgren K., Walworth N., Booher R., Dembski M., Kirschner M., Beach D.
      Cell 64:1111-1122(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.

    Entry informationi

    Entry nameiMIK1_SCHPO
    AccessioniPrimary (citable) accession number: P30290
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3