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P30289

- RNS3_STRAU

UniProt

P30289 - RNS3_STRAU

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Protein
Guanyl-specific ribonuclease Sa3
Gene
rnaSA3
Organism
Streptomyces aureofaciens
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in G-P with 2',3'-cyclic phosphate intermediates.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei99 – 991Proton acceptor
Active sitei130 – 1301Proton donor

GO - Molecular functioni

  1. RNA binding Source: InterPro
  2. endoribonuclease activity Source: InterPro
  3. ribonuclease T1 activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanyl-specific ribonuclease Sa3 (EC:3.1.27.3)
    Short name:
    RNase Sa3
    Gene namesi
    Name:rnaSA3
    OrganismiStreptomyces aureofaciens
    Taxonomic identifieri1894 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3636Or 43 Reviewed prediction
    Add
    BLAST
    Chaini37 – 141105Guanyl-specific ribonuclease Sa3
    PRO_0000030830Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi52 ↔ 141 By similarity

    Keywords - PTMi

    Disulfide bond

    Structurei

    Secondary structure

    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi49 – 524
    Helixi53 – 553
    Helixi58 – 6912
    Turni76 – 794
    Beta strandi98 – 1014
    Beta strandi114 – 1174
    Beta strandi124 – 1296
    Beta strandi135 – 1384

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1MGRX-ray1.70A43-141[»]
    1MGWX-ray2.00A43-141[»]
    ProteinModelPortaliP30289.
    SMRiP30289. Positions 43-141.

    Miscellaneous databases

    EvolutionaryTraceiP30289.

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.10.450.30. 1 hit.
    InterProiIPR000026. Gua-sp_ribonuclease_N1/T1.
    IPR016191. Ribonuclease/ribotoxin.
    [Graphical view]
    PfamiPF00545. Ribonuclease. 1 hit.
    [Graphical view]
    SUPFAMiSSF53933. SSF53933. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P30289-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRIPPRLVAL AGAAAVAATL IAGPVAAAAP ASHAVAASSA ASASVKAVGR    50
    VCYSALPSQA HDTLDLIDEG GPFPYSQDGV VFQNREGLLP AHSTGYYHEY 100
    TVITPGSPTR GARRIITGQQ WQEDYYTADH YASFRRVDFA C 141
    Length:141
    Mass (Da):14,820
    Last modified:April 1, 1993 - v1
    Checksum:iDB128BB3E60FDCF6
    GO

    Sequence cautioni

    The sequence AAA26809.1 differs from that shown. Reason: Frameshift at several positions.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M82920 Genomic DNA. Translation: AAA26809.1. Frameshift.
    PIRiJC1287.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M82920 Genomic DNA. Translation: AAA26809.1 . Frameshift.
    PIRi JC1287.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1MGR X-ray 1.70 A 43-141 [» ]
    1MGW X-ray 2.00 A 43-141 [» ]
    ProteinModelPortali P30289.
    SMRi P30289. Positions 43-141.
    ModBasei Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P30289.

    Family and domain databases

    Gene3Di 3.10.450.30. 1 hit.
    InterProi IPR000026. Gua-sp_ribonuclease_N1/T1.
    IPR016191. Ribonuclease/ribotoxin.
    [Graphical view ]
    Pfami PF00545. Ribonuclease. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53933. SSF53933. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of the gene encoding a ribonuclease from Streptomyces aureofaciens CCM3239."
      Homerova D., Hollaenderova Z., Kormanec J., Sevcik J.
      Gene 119:147-148(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 10762 / CCM 3239 / DSM 40127 / JCM 4008 / LMG 5968.
    2. "X-ray structure of two crystalline forms of a streptomycete ribonuclease with cytotoxic activity."
      Sevcik J., Urbanikova L., Leland P.A., Raines R.T.
      J. Biol. Chem. 277:47325-47330(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 45-141.

    Entry informationi

    Entry nameiRNS3_STRAU
    AccessioniPrimary (citable) accession number: P30289
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: April 16, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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