P30289 (RNS3_STRAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanyl-specific ribonuclease Sa3
Short name=RNase Sa3
EC=3.1.27.3

Gene names

Name:

rnaSA3

Organism

Streptomyces aureofaciens

Taxonomic identifier

1894 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	141 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in G-P with 2',3'-cyclic phosphate intermediates.
Subcellular location	Secreted.
Sequence similarities	Belongs to the ribonuclease N1/T1 family.
Sequence caution	The sequence AAA26809.1 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond
Technical term	3D-structure
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro endoribonuclease activity Inferred from electronic annotation. Source: InterPro ribonuclease T1 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 36

36

Or 43 Potential

Chain

37 – 141

105

Guanyl-specific ribonuclease Sa3

PRO_0000030830

Sites

Active site

99

1

Proton acceptor

Active site

130

1

Proton donor

Amino acid modifications

Disulfide bond

52 ↔ 141

By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

141

Helix Strand Turn

Details...

Beta strand

49 – 52

4

Helix

53 – 55

3

Helix

58 – 69

12

Turn

76 – 79

4

Beta strand

98 – 101

4

Beta strand

114 – 117

4

Beta strand

124 – 129

6

Beta strand

135 – 138

4

Sequences

	Sequence		Length	Mass (Da)
	P30289 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: DB128BB3E60FDCF6	FASTA	141	14,820
	10 20 30 40 50 60 MRIPPRLVAL AGAAAVAATL IAGPVAAAAP ASHAVAASSA ASASVKAVGR VCYSALPSQA 70 80 90 100 110 120 HDTLDLIDEG GPFPYSQDGV VFQNREGLLP AHSTGYYHEY TVITPGSPTR GARRIITGQQ 130 140 WQEDYYTADH YASFRRVDFA C

References

[1]	"Cloning and sequencing of the gene encoding a ribonuclease from Streptomyces aureofaciens CCM3239." Homerova D., Hollaenderova Z., Kormanec J., Sevcik J. Gene 119:147-148(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 10762 / CCM 3239 / DSM 40127 / JCM 4008 / LMG 5968.
[2]	"X-ray structure of two crystalline forms of a streptomycete ribonuclease with cytotoxic activity." Sevcik J., Urbanikova L., Leland P.A., Raines R.T. J. Biol. Chem. 277:47325-47330(2002) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 45-141.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M82920 Genomic DNA. Translation: AAA26809.1. Frameshift.

PIR

JC1287.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1MGR	X-ray	1.70	A	43-141	[»]
1MGW	X-ray	2.00	A	43-141	[»]

ProteinModelPortal

P30289.

SMR

P30289. Positions 43-141.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

3.10.450.30. 1 hit.

InterPro

IPR000026. Gua-sp_ribonuclease_N1/T1.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view]

Pfam

PF00545. Ribonuclease. 1 hit.
[Graphical view]

SUPFAM

SSF53933. Ribonuclease/ribotoxin. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P30289.

Entry information

Entry name

RNS3_STRAU

Accession

Primary (citable) accession number: P30289

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	April 3, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program