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P30289

- RNS3_STRAU

UniProt

P30289 - RNS3_STRAU

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Protein

Guanyl-specific ribonuclease Sa3

Gene

rnaSA3

Organism
Streptomyces aureofaciens
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in G-P with 2',3'-cyclic phosphate intermediates.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei99 – 991Proton acceptor
Active sitei130 – 1301Proton donor

GO - Molecular functioni

  1. endoribonuclease activity Source: InterPro
  2. ribonuclease T1 activity Source: UniProtKB-EC
  3. RNA binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Names & Taxonomyi

Protein namesi
Recommended name:
Guanyl-specific ribonuclease Sa3 (EC:3.1.27.3)
Short name:
RNase Sa3
Gene namesi
Name:rnaSA3
OrganismiStreptomyces aureofaciens
Taxonomic identifieri1894 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3636Or 43Sequence AnalysisAdd
BLAST
Chaini37 – 141105Guanyl-specific ribonuclease Sa3PRO_0000030830Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi52 ↔ 141By similarity

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

1
141
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi49 – 524
Helixi53 – 553
Helixi58 – 6912
Turni76 – 794
Beta strandi98 – 1014
Beta strandi114 – 1174
Beta strandi124 – 1296
Beta strandi135 – 1384

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MGRX-ray1.70A43-141[»]
1MGWX-ray2.00A43-141[»]
ProteinModelPortaliP30289.
SMRiP30289. Positions 43-141.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP30289.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribonuclease N1/T1 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.10.450.30. 1 hit.
InterProiIPR000026. Gua-sp_ribonuclease_N1/T1.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view]
PfamiPF00545. Ribonuclease. 1 hit.
[Graphical view]
SUPFAMiSSF53933. SSF53933. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30289-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRIPPRLVAL AGAAAVAATL IAGPVAAAAP ASHAVAASSA ASASVKAVGR
60 70 80 90 100
VCYSALPSQA HDTLDLIDEG GPFPYSQDGV VFQNREGLLP AHSTGYYHEY
110 120 130 140
TVITPGSPTR GARRIITGQQ WQEDYYTADH YASFRRVDFA C
Length:141
Mass (Da):14,820
Last modified:April 1, 1993 - v1
Checksum:iDB128BB3E60FDCF6
GO

Sequence cautioni

The sequence AAA26809.1 differs from that shown. Reason: Frameshift at several positions.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M82920 Genomic DNA. Translation: AAA26809.1. Frameshift.
PIRiJC1287.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M82920 Genomic DNA. Translation: AAA26809.1 . Frameshift.
PIRi JC1287.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1MGR X-ray 1.70 A 43-141 [» ]
1MGW X-ray 2.00 A 43-141 [» ]
ProteinModelPortali P30289.
SMRi P30289. Positions 43-141.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P30289.

Family and domain databases

Gene3Di 3.10.450.30. 1 hit.
InterProi IPR000026. Gua-sp_ribonuclease_N1/T1.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view ]
Pfami PF00545. Ribonuclease. 1 hit.
[Graphical view ]
SUPFAMi SSF53933. SSF53933. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequencing of the gene encoding a ribonuclease from Streptomyces aureofaciens CCM3239."
    Homerova D., Hollaenderova Z., Kormanec J., Sevcik J.
    Gene 119:147-148(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 10762 / CCM 3239 / DSM 40127 / JCM 4008 / LMG 5968.
  2. "X-ray structure of two crystalline forms of a streptomycete ribonuclease with cytotoxic activity."
    Sevcik J., Urbanikova L., Leland P.A., Raines R.T.
    J. Biol. Chem. 277:47325-47330(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 45-141.

Entry informationi

Entry nameiRNS3_STRAU
AccessioniPrimary (citable) accession number: P30289
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: October 29, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3