Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P30289 (RNS3_STRAU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Guanyl-specific ribonuclease Sa3

Short name=RNase Sa3
EC=3.1.27.3
Gene names
Name:rnaSA3
OrganismStreptomyces aureofaciens
Taxonomic identifier1894 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Protein attributes

Sequence length141 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in G-P with 2',3'-cyclic phosphate intermediates.

Subcellular location

Secreted.

Sequence similarities

Belongs to the ribonuclease N1/T1 family.

Sequence caution

The sequence AAA26809.1 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionEndonuclease
Hydrolase
Nuclease
   PTMDisulfide bond
   Technical term3D-structure
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: InterPro

endoribonuclease activity

Inferred from electronic annotation. Source: InterPro

ribonuclease T1 activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3636Or 43 Potential
Chain37 – 141105Guanyl-specific ribonuclease Sa3
PRO_0000030830

Sites

Active site991Proton acceptor
Active site1301Proton donor

Amino acid modifications

Disulfide bond52 ↔ 141 By similarity

Secondary structure

................ 141
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P30289 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: DB128BB3E60FDCF6

FASTA14114,820
        10         20         30         40         50         60 
MRIPPRLVAL AGAAAVAATL IAGPVAAAAP ASHAVAASSA ASASVKAVGR VCYSALPSQA 

        70         80         90        100        110        120 
HDTLDLIDEG GPFPYSQDGV VFQNREGLLP AHSTGYYHEY TVITPGSPTR GARRIITGQQ 

       130        140 
WQEDYYTADH YASFRRVDFA C 

« Hide

References

[1]"Cloning and sequencing of the gene encoding a ribonuclease from Streptomyces aureofaciens CCM3239."
Homerova D., Hollaenderova Z., Kormanec J., Sevcik J.
Gene 119:147-148(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 10762 / CCM 3239 / DSM 40127 / JCM 4008 / LMG 5968.
[2]"X-ray structure of two crystalline forms of a streptomycete ribonuclease with cytotoxic activity."
Sevcik J., Urbanikova L., Leland P.A., Raines R.T.
J. Biol. Chem. 277:47325-47330(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 45-141.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M82920 Genomic DNA. Translation: AAA26809.1. Frameshift.
PIRJC1287.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1MGRX-ray1.70A43-141[»]
1MGWX-ray2.00A43-141[»]
ProteinModelPortalP30289.
SMRP30289. Positions 43-141.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.10.450.30. 1 hit.
InterProIPR000026. Gua-sp_ribonuclease_N1/T1.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view]
PfamPF00545. Ribonuclease. 1 hit.
[Graphical view]
SUPFAMSSF53933. SSF53933. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP30289.

Entry information

Entry nameRNS3_STRAU
AccessionPrimary (citable) accession number: P30289
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: April 16, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references