Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Omega-agatoxin-Aa4a

Gene
N/A
Organism
Agelenopsis aperta (North American funnel-web spider) (Agelenopsis gertschi)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Omega-agatoxins inhibit neuronal voltage-gated calcium channels. This toxin acts by modifying the gating of the high voltage activated P-type Cav2.1/CACNA1A channel. Is a potent blocker in both insect and mammalian central neurons.7 Publications

GO - Molecular functioni

  • calcium channel inhibitor activity Source: CACAO

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Calcium channel impairing toxin, Ion channel impairing toxin, Neurotoxin, Presynaptic neurotoxin, Toxin, Voltage-gated calcium channel impairing toxin

Protein family/group databases

TCDBi8.B.6.1.1. the ca(2+) channel-targeting spider toxin (cst) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Omega-agatoxin-Aa4a
Short name:
Omega-AGTX-Aa4a
Alternative name(s):
Omega-agatoxin IVA
Short name:
Omega-Aga-IVA
Omega-agatoxin-4A
OrganismiAgelenopsis aperta (North American funnel-web spider) (Agelenopsis gertschi)
Taxonomic identifieri6908 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeAraneomorphaeEntelegynaeAgelenidaeAgelenopsis

Organism-specific databases

ArachnoServeriAS000182. omega-agatoxin-Aa4a.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4848Omega-agatoxin-Aa4aPRO_0000087610Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi4 ↔ 20
Disulfide bondi12 ↔ 25
Disulfide bondi19 ↔ 36
Disulfide bondi27 ↔ 34

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.1 Publication

Structurei

Secondary structure

1
48
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 53Combined sources
Beta strandi7 – 115Combined sources
Beta strandi20 – 223Combined sources
Beta strandi24 – 263Combined sources
Beta strandi29 – 335Combined sources
Beta strandi35 – 384Combined sources
Turni41 – 444Combined sources
Turni45 – 473Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IVANMR-A1-48[»]
1OAVNMR-A1-48[»]
1OAWNMR-A1-48[»]
ProteinModelPortaliP30288.
SMRiP30288. Positions 1-48.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP30288.

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Sequence similaritiesi

Keywords - Domaini

Knottin

Family and domain databases

InterProiIPR004169. Spidertoxin.
[Graphical view]
PfamiPF02819. Toxin_9. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30288-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40 
KKKCIAKDYG RCKWGGTPCC RGRGCICSIM GTNCECKPRL IMEGLGLA
Length:48
Mass (Da):5,210
Last modified:April 1, 1993 - v1
Checksum:i945054B55EAE81FD
GO

Sequence databases

PIRiS20256.

Cross-referencesi

Sequence databases

PIRiS20256.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IVANMR-A1-48[»]
1OAVNMR-A1-48[»]
1OAWNMR-A1-48[»]
ProteinModelPortaliP30288.
SMRiP30288. Positions 1-48.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi8.B.6.1.1. the ca(2+) channel-targeting spider toxin (cst) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

ArachnoServeriAS000182. omega-agatoxin-Aa4a.

Miscellaneous databases

EvolutionaryTraceiP30288.

Family and domain databases

InterProiIPR004169. Spidertoxin.
[Graphical view]
PfamiPF02819. Toxin_9. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTOG4A_AGEAP
AccessioniPrimary (citable) accession number: P30288
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: July 6, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Miscellaneous

This toxin is a diagnostic ligand for P-type channels Ca2.1/CACNA1A in the mammalian brain.
The binding site for this toxin has been localized in part to the extracellular S3-S4 loop in repeat IV of the alpha1 subunit of mammalian Cav2.1/CACNA1A channels, which is proximal to the S4 sensor domain.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.