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Protein

G1/S-specific cyclin-D3

Gene

Ccnd3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

GO - Molecular functioni

  1. cyclin-dependent protein serine/threonine kinase activity Source: MGI
  2. protein kinase binding Source: MGI

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. positive regulation of cyclin-dependent protein serine/threonine kinase activity Source: MGI
  4. positive regulation of protein phosphorylation Source: MGI
  5. regulation of cell cycle Source: MGI
  6. signal transduction Source: MGI
  7. T cell proliferation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Cyclin

Keywords - Biological processi

Cell cycle, Cell division

Enzyme and pathway databases

ReactomeiREACT_252217. Transcriptional regulation of white adipocyte differentiation.

Names & Taxonomyi

Protein namesi
Recommended name:
G1/S-specific cyclin-D3
Gene namesi
Name:Ccnd3
Synonyms:Cyl-3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:88315. Ccnd3.

Subcellular locationi

Nucleus By similarity. Cytoplasm By similarity. Membrane By similarity
Note: Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members.By similarity

GO - Cellular componenti

  1. cyclin-dependent protein kinase holoenzyme complex Source: MGI
  2. cytoplasm Source: UniProtKB-SubCell
  3. membrane Source: UniProtKB-SubCell
  4. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 292292G1/S-specific cyclin-D3PRO_0000080443Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei279 – 2791PhosphoserineBy similarity

Post-translational modificationi

Polyubiquitinated by the SCF(FBXL2) complex, leading to proteasomal degradation.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP30282.
PaxDbiP30282.
PRIDEiP30282.

PTM databases

PhosphoSiteiP30282.

Expressioni

Gene expression databases

BgeeiP30282.
CleanExiMM_CCND3.
ExpressionAtlasiP30282. baseline and differential.
GenevestigatoriP30282.

Interactioni

Subunit structurei

Interacts with the CDK4 and CDK6 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Component of the ternary complex cyclin D/CDK4/CDKN1B required for nuclear translocation and modulation of CDK4-mediated kinase activity. Can form similar complexes with either CDKN1A or CDKN2A. Interacts with ATF5 and EIF3K (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Cdk6Q642612EBI-847337,EBI-847380

Protein-protein interaction databases

BioGridi198550. 10 interactions.
IntActiP30282. 5 interactions.
MINTiMINT-1510794.

Structurei

3D structure databases

ProteinModelPortaliP30282.
SMRiP30282. Positions 23-254.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 152126Cyclin N-terminalAdd
BLAST

Sequence similaritiesi

Belongs to the cyclin family. Cyclin D subfamily.Curated
Contains 1 cyclin N-terminal domain.Curated

Phylogenomic databases

eggNOGiNOG294373.
HOGENOMiHOG000008182.
HOVERGENiHBG050837.
InParanoidiP30282.
KOiK10152.
OMAiLAFWMLE.
OrthoDBiEOG7J447H.
PhylomeDBiP30282.
TreeFamiTF101004.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR013763. Cyclin-like.
IPR014400. Cyclin_A/B/D/E/F.
IPR004367. Cyclin_C-dom.
IPR015451. Cyclin_D.
IPR006671. Cyclin_N.
[Graphical view]
PANTHERiPTHR10177:SF65. PTHR10177:SF65. 1 hit.
PfamiPF02984. Cyclin_C. 1 hit.
PF00134. Cyclin_N. 1 hit.
[Graphical view]
PIRSFiPIRSF001771. Cyclin_A_B_D_E. 1 hit.
SMARTiSM00385. CYCLIN. 1 hit.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS00292. CYCLINS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30282-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELLCCEGTR HAPRAGPDPR LLGDQRVLQS LLRLEERYVP RASYFQCVQK
60 70 80 90 100
EIKPHMRKML AYWMLEVCEE QRCEEDVFPL AMNYLDRYLS CVPTRKAQLQ
110 120 130 140 150
LLGTVCLLLA SKLRETTPLT IEKLCIYTDQ AVAPWQLREW EVLVLGKLKW
160 170 180 190 200
DLAAVIAHDF LALILHRLSL PSDRQALVKK HAQTFLALCA TDYTFAMYPP
210 220 230 240 250
SMIATGSIGA AVLGLGACSM SADELTELLA GITGTEVDCL RACQEQIEAA
260 270 280 290
LRESLREAAQ TAPSPVPKAP RGSSSQGPSQ TSTPTDVTAI HL
Length:292
Mass (Da):32,411
Last modified:April 1, 1993 - v1
Checksum:i5441639A9B1B1475
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti239 – 2391C → Y in AAH04076 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86183 mRNA. Translation: AAA37504.1.
U43844 Genomic DNA. Translation: AAC53363.1.
BC004076 mRNA. Translation: AAH04076.1.
BC005605 mRNA. Translation: AAH05605.1.
CCDSiCCDS28850.1.
PIRiC40035.
RefSeqiNP_001075104.1. NM_001081635.1.
NP_001075105.1. NM_001081636.1.
NP_031658.1. NM_007632.2.
UniGeneiMm.27291.

Genome annotation databases

EnsembliENSMUST00000037333; ENSMUSP00000040488; ENSMUSG00000034165.
ENSMUST00000171031; ENSMUSP00000126141; ENSMUSG00000034165.
ENSMUST00000182209; ENSMUSP00000138091; ENSMUSG00000034165.
ENSMUST00000183044; ENSMUSP00000138220; ENSMUSG00000034165.
ENSMUST00000183177; ENSMUSP00000138640; ENSMUSG00000034165.
GeneIDi12445.
KEGGimmu:12445.
UCSCiuc008cvk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86183 mRNA. Translation: AAA37504.1.
U43844 Genomic DNA. Translation: AAC53363.1.
BC004076 mRNA. Translation: AAH04076.1.
BC005605 mRNA. Translation: AAH05605.1.
CCDSiCCDS28850.1.
PIRiC40035.
RefSeqiNP_001075104.1. NM_001081635.1.
NP_001075105.1. NM_001081636.1.
NP_031658.1. NM_007632.2.
UniGeneiMm.27291.

3D structure databases

ProteinModelPortaliP30282.
SMRiP30282. Positions 23-254.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198550. 10 interactions.
IntActiP30282. 5 interactions.
MINTiMINT-1510794.

PTM databases

PhosphoSiteiP30282.

Proteomic databases

MaxQBiP30282.
PaxDbiP30282.
PRIDEiP30282.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000037333; ENSMUSP00000040488; ENSMUSG00000034165.
ENSMUST00000171031; ENSMUSP00000126141; ENSMUSG00000034165.
ENSMUST00000182209; ENSMUSP00000138091; ENSMUSG00000034165.
ENSMUST00000183044; ENSMUSP00000138220; ENSMUSG00000034165.
ENSMUST00000183177; ENSMUSP00000138640; ENSMUSG00000034165.
GeneIDi12445.
KEGGimmu:12445.
UCSCiuc008cvk.1. mouse.

Organism-specific databases

CTDi896.
MGIiMGI:88315. Ccnd3.

Phylogenomic databases

eggNOGiNOG294373.
HOGENOMiHOG000008182.
HOVERGENiHBG050837.
InParanoidiP30282.
KOiK10152.
OMAiLAFWMLE.
OrthoDBiEOG7J447H.
PhylomeDBiP30282.
TreeFamiTF101004.

Enzyme and pathway databases

ReactomeiREACT_252217. Transcriptional regulation of white adipocyte differentiation.

Miscellaneous databases

ChiTaRSiCcnd3. mouse.
NextBioi281262.
PROiP30282.
SOURCEiSearch...

Gene expression databases

BgeeiP30282.
CleanExiMM_CCND3.
ExpressionAtlasiP30282. baseline and differential.
GenevestigatoriP30282.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR013763. Cyclin-like.
IPR014400. Cyclin_A/B/D/E/F.
IPR004367. Cyclin_C-dom.
IPR015451. Cyclin_D.
IPR006671. Cyclin_N.
[Graphical view]
PANTHERiPTHR10177:SF65. PTHR10177:SF65. 1 hit.
PfamiPF02984. Cyclin_C. 1 hit.
PF00134. Cyclin_N. 1 hit.
[Graphical view]
PIRSFiPIRSF001771. Cyclin_A_B_D_E. 1 hit.
SMARTiSM00385. CYCLIN. 1 hit.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS00292. CYCLINS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Colony-stimulating factor 1 regulates novel cyclins during the G1 phase of the cell cycle."
    Matsushime H., Roussel M.F., Ashmun R.A., Sherr C.J.
    Cell 65:701-713(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Characterization of the mouse cyclin D3 gene: exon/intron organization and promoter activity."
    Wang Z., Sicinski P., Weinberg R.A., Zhang Y., Ravid K.
    Genomics 35:156-163(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Sv.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Mammary gland.

Entry informationi

Entry nameiCCND3_MOUSE
AccessioniPrimary (citable) accession number: P30282
Secondary accession number(s): Q62391, Q99KP2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: March 4, 2015
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.