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Protein

G1/S-specific cyclin-D3

Gene

Ccnd3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G1 phase. Hypophosphorylates RB1 in early G1 phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

GO - Molecular functioni

  • cyclin-dependent protein serine/threonine kinase activity Source: MGI
  • protein kinase binding Source: MGI

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • positive regulation of cyclin-dependent protein serine/threonine kinase activity Source: MGI
  • positive regulation of protein phosphorylation Source: MGI
  • regulation of cell cycle Source: MGI
  • regulation of cell proliferation Source: MGI
  • regulation of insulin receptor signaling pathway Source: MGI
  • signal transduction Source: MGI
  • T cell proliferation Source: MGI

Keywordsi

Molecular functionCyclin
Biological processCell cycle, Cell division

Enzyme and pathway databases

ReactomeiR-MMU-5687128. MAPK6/MAPK4 signaling.
R-MMU-8934593. Regulation of RUNX1 Expression and Activity.

Names & Taxonomyi

Protein namesi
Recommended name:
G1/S-specific cyclin-D3
Gene namesi
Name:Ccnd3
Synonyms:Cyl-3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:88315. Ccnd3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000804431 – 292G1/S-specific cyclin-D3Add BLAST292

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei264PhosphoserineCombined sources1
Modified residuei279PhosphoserineBy similarity1

Post-translational modificationi

Polyubiquitinated by the SCF(FBXL2) complex, leading to proteasomal degradation.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP30282.
PaxDbiP30282.
PeptideAtlasiP30282.
PRIDEiP30282.

PTM databases

iPTMnetiP30282.
PhosphoSitePlusiP30282.

Expressioni

Gene expression databases

BgeeiENSMUSG00000034165.
CleanExiMM_CCND3.
ExpressionAtlasiP30282. baseline and differential.
GenevisibleiP30282. MM.

Interactioni

Subunit structurei

Interacts with the CDK4 and CDK6 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Component of the ternary complex cyclin D/CDK4/CDKN1B required for nuclear translocation and modulation of CDK4-mediated kinase activity. Can form similar complexes with either CDKN1A or CDKN2A. Interacts with ATF5 and EIF3K (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Cdk6Q642612EBI-847337,EBI-847380

GO - Molecular functioni

  • protein kinase binding Source: MGI

Protein-protein interaction databases

BioGridi198550. 10 interactors.
IntActiP30282. 6 interactors.
MINTiMINT-1510794.
STRINGi10090.ENSMUSP00000040488.

Structurei

3D structure databases

ProteinModelPortaliP30282.
SMRiP30282.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 152Cyclin N-terminalAdd BLAST126

Sequence similaritiesi

Belongs to the cyclin family. Cyclin D subfamily.Curated

Phylogenomic databases

eggNOGiKOG0656. Eukaryota.
ENOG410XRKC. LUCA.
GeneTreeiENSGT00760000118939.
HOGENOMiHOG000008182.
HOVERGENiHBG050837.
InParanoidiP30282.
KOiK10152.
OMAiPYMRKML.
OrthoDBiEOG091G0URX.
PhylomeDBiP30282.
TreeFamiTF101004.

Family and domain databases

CDDicd00043. CYCLIN. 1 hit.
Gene3Di1.10.472.10. 2 hits.
InterProiView protein in InterPro
IPR013763. Cyclin-like.
IPR036915. Cyclin-like_sf.
IPR004367. Cyclin_C-dom.
IPR015451. Cyclin_D.
IPR006671. Cyclin_N.
PANTHERiPTHR10177:SF65. PTHR10177:SF65. 1 hit.
PfamiView protein in Pfam
PF02984. Cyclin_C. 1 hit.
PF00134. Cyclin_N. 1 hit.
SMARTiView protein in SMART
SM00385. CYCLIN. 1 hit.
SM01332. Cyclin_C. 1 hit.
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiView protein in PROSITE
PS00292. CYCLINS. 1 hit.

Sequencei

Sequence statusi: Complete.

P30282-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELLCCEGTR HAPRAGPDPR LLGDQRVLQS LLRLEERYVP RASYFQCVQK
60 70 80 90 100
EIKPHMRKML AYWMLEVCEE QRCEEDVFPL AMNYLDRYLS CVPTRKAQLQ
110 120 130 140 150
LLGTVCLLLA SKLRETTPLT IEKLCIYTDQ AVAPWQLREW EVLVLGKLKW
160 170 180 190 200
DLAAVIAHDF LALILHRLSL PSDRQALVKK HAQTFLALCA TDYTFAMYPP
210 220 230 240 250
SMIATGSIGA AVLGLGACSM SADELTELLA GITGTEVDCL RACQEQIEAA
260 270 280 290
LRESLREAAQ TAPSPVPKAP RGSSSQGPSQ TSTPTDVTAI HL
Length:292
Mass (Da):32,411
Last modified:April 1, 1993 - v1
Checksum:i5441639A9B1B1475
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti239C → Y in AAH04076 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86183 mRNA. Translation: AAA37504.1.
U43844 Genomic DNA. Translation: AAC53363.1.
BC004076 mRNA. Translation: AAH04076.1.
BC005605 mRNA. Translation: AAH05605.1.
CCDSiCCDS28850.1.
PIRiC40035.
RefSeqiNP_001075104.1. NM_001081635.1.
NP_001075105.1. NM_001081636.1.
NP_031658.1. NM_007632.2.
UniGeneiMm.27291.

Genome annotation databases

EnsembliENSMUST00000037333; ENSMUSP00000040488; ENSMUSG00000034165.
ENSMUST00000171031; ENSMUSP00000126141; ENSMUSG00000034165.
ENSMUST00000182209; ENSMUSP00000138091; ENSMUSG00000034165.
ENSMUST00000183044; ENSMUSP00000138220; ENSMUSG00000034165.
ENSMUST00000183177; ENSMUSP00000138640; ENSMUSG00000034165.
GeneIDi12445.
KEGGimmu:12445.
UCSCiuc008cvk.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiCCND3_MOUSE
AccessioniPrimary (citable) accession number: P30282
Secondary accession number(s): Q62391, Q99KP2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: November 22, 2017
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families