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Protein

G2/mitotic-specific cyclin-B1

Gene

Ccnb1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Essential for the control of the cell cycle at the G2/M (mitosis) transition.

GO - Molecular functioni

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • cellular response to fatty acid Source: RGD
  • cellular response to hypoxia Source: RGD
  • cellular response to iron(III) ion Source: RGD
  • cellular response to organic cyclic compound Source: RGD
  • digestive tract development Source: RGD
  • histone H3-S10 phosphorylation involved in chromosome condensation Source: Ensembl
  • in utero embryonic development Source: Ensembl
  • mitotic cell cycle Source: RGD
  • mitotic metaphase plate congression Source: Ensembl
  • mitotic spindle organization Source: Ensembl
  • negative regulation of gene expression Source: RGD
  • negative regulation of protein phosphorylation Source: RGD
  • oocyte maturation Source: RGD
  • positive regulation of attachment of spindle microtubules to kinetochore Source: Ensembl
  • positive regulation of cardiac muscle cell proliferation Source: RGD
  • positive regulation of fibroblast proliferation Source: Ensembl
  • positive regulation of G2/M transition of mitotic cell cycle Source: Ensembl
  • positive regulation of histone phosphorylation Source: RGD
  • positive regulation of mitochondrial ATP synthesis coupled electron transport Source: Ensembl
  • positive regulation of mRNA 3'-end processing Source: RGD
  • protein-containing complex assembly Source: RGD
  • regulation of cell cycle Source: RGD
  • regulation of chromosome condensation Source: RGD
  • regulation of mitotic cell cycle spindle assembly checkpoint Source: Ensembl
  • response to DDT Source: RGD
  • response to drug Source: RGD
  • response to mechanical stimulus Source: RGD
  • response to toxic substance Source: RGD
  • spermatogenesis Source: RGD
  • tissue regeneration Source: RGD
  • ventricular cardiac muscle cell development Source: RGD

Keywordsi

Molecular functionCyclin
Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-RNO-174048 APC/C:Cdc20 mediated degradation of Cyclin B
R-RNO-176408 Regulation of APC/C activators between G1/S and early anaphase
R-RNO-176412 Phosphorylation of the APC/C
R-RNO-176417 Phosphorylation of Emi1
R-RNO-2299718 Condensation of Prophase Chromosomes
R-RNO-2500257 Resolution of Sister Chromatid Cohesion
R-RNO-2565942 Regulation of PLK1 Activity at G2/M Transition
R-RNO-2980767 Activation of NIMA Kinases NEK9, NEK6, NEK7
R-RNO-3301854 Nuclear Pore Complex (NPC) Disassembly
R-RNO-4419969 Depolymerisation of the Nuclear Lamina
R-RNO-6804114 TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest
R-RNO-68875 Mitotic Prophase
R-RNO-69273 Cyclin A/B1/B2 associated events during G2/M transition
R-RNO-69478 G2/M DNA replication checkpoint
R-RNO-75035 Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex
R-RNO-8852276 The role of GTSE1 in G2/M progression after G2 checkpoint
R-RNO-8878166 Transcriptional regulation by RUNX2

Names & Taxonomyi

Protein namesi
Recommended name:
G2/mitotic-specific cyclin-B1
Gene namesi
Name:Ccnb1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi2291 Ccnb1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2093

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000803531 – 423G2/mitotic-specific cyclin-B1Add BLAST423

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei116Phosphoserine; by CDK1By similarity1
Modified residuei118PhosphoserineBy similarity1
Modified residuei123Phosphoserine; by PLK1By similarity1
Modified residuei137PhosphoserineBy similarity1
Modified residuei311PhosphothreonineBy similarity1

Post-translational modificationi

Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases (By similarity).By similarity
Phosphorylated by PLK1 at Ser-123 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-137 was also reported to be mediated by PLK1 but Ser-123 seems to be the primary phosphorylation site (By similarity).By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP30277
PRIDEiP30277

PTM databases

iPTMnetiP30277
PhosphoSitePlusiP30277

Expressioni

Developmental stagei

Accumulates steadily during G2 and is abruptly destroyed at mitosis.

Gene expression databases

BgeeiENSRNOG00000058539
GenevisibleiP30277 RN

Interactioni

Subunit structurei

Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase. Interacts with CCNF; interaction is required for nuclear localization. Interacts with CDK5RAP3.By similarity

GO - Molecular functioni

Protein-protein interaction databases

CORUMiP30277
IntActiP30277, 1 interactor
STRINGi10116.ENSRNOP00000025297

Chemistry databases

BindingDBiP30277

Structurei

3D structure databases

ProteinModelPortaliP30277
SMRiP30277
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni159 – 167Interaction with CDK2By similarity9
Regioni248 – 251Interaction with CDK2By similarity4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi50 – 82Lys-richAdd BLAST33

Sequence similaritiesi

Belongs to the cyclin family. Cyclin AB subfamily.Curated

Phylogenomic databases

eggNOGiKOG0653 Eukaryota
COG5024 LUCA
GeneTreeiENSGT00760000118939
HOGENOMiHOG000167672
HOVERGENiHBG061650
InParanoidiP30277
KOiK05868
OMAiHMTVKNK
OrthoDBiEOG091G0E9B
PhylomeDBiP30277
TreeFamiTF101001

Family and domain databases

CDDicd00043 CYCLIN, 2 hits
InterProiView protein in InterPro
IPR013763 Cyclin-like
IPR036915 Cyclin-like_sf
IPR004367 Cyclin_C-dom
IPR006671 Cyclin_N
PfamiView protein in Pfam
PF02984 Cyclin_C, 1 hit
PF00134 Cyclin_N, 1 hit
SMARTiView protein in SMART
SM00385 CYCLIN, 2 hits
SM01332 Cyclin_C, 1 hit
SUPFAMiSSF47954 SSF47954, 2 hits
PROSITEiView protein in PROSITE
PS00292 CYCLINS, 1 hit

Sequencei

Sequence statusi: Complete.

P30277-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALRVTRNTK INTENKAKVS MAGAKRVPVA VAASKPLLRS RTALGDIGNK
60 70 80 90 100
VSEQSRIPLK KETKKLGSGT VTVKALPKPV DKVPVCEPEV ELDEPEPEPV
110 120 130 140 150
MEVKHSPEPI LVDTPSPSPM ETSGCAPAEE YLCQAFSDVI LAVSDVDADD
160 170 180 190 200
GGDPNLCSEY VKDIYAYLRQ LEEEQSVRPK YLLGREVTGN MRAILIDWLI
210 220 230 240 250
QVQMKFRLLQ ETMYMTVSII DRFMQDSCVP KKMLQLVGVT AMFIASKYEE
260 270 280 290 300
MYPPEIGDFA FVTNNTYTKH QIRQMEMKIL RVLNFSLGRP LPLHFLRRAS
310 320 330 340 350
KIGEVDVEQH TLAKYLMELS MLDYDMVHFA PSQIAAGAFC LALKILDNGE
360 370 380 390 400
WTPTLQHYLS HTEESLLPVM QHLAKNIVMV NRGLTKHMTI KNKYATSKHA
410 420
KISTLAQLNC TLVQNLSKAV TKA
Length:423
Mass (Da):47,391
Last modified:April 1, 1993 - v1
Checksum:i4223CF71F144B279
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60768 mRNA Translation: CAA43178.1
X64589 mRNA Translation: CAA45877.1
L11995 mRNA Translation: AAC00032.1
BC059113 mRNA Translation: AAH59113.1
PIRiS34226
RefSeqiNP_741988.1, NM_171991.3
UniGeneiRn.9232

Genome annotation databases

EnsembliENSRNOT00000082846; ENSRNOP00000075114; ENSRNOG00000058539
GeneIDi25203
KEGGirno:25203
UCSCiRGD:2291 rat

Similar proteinsi

Entry informationi

Entry nameiCCNB1_RAT
AccessioniPrimary (citable) accession number: P30277
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: May 23, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health