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Protein

G2/mitotic-specific cyclin-B1

Gene

Ccnb1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Essential for the control of the cell cycle at the G2/M (mitosis) transition.

GO - Molecular functioni

  • kinase activity Source: Ensembl
  • protein kinase binding Source: RGD

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • cellular response to fatty acid Source: RGD
  • cellular response to hypoxia Source: RGD
  • cellular response to iron(III) ion Source: RGD
  • cellular response to organic cyclic compound Source: RGD
  • digestive tract development Source: RGD
  • histone H3-S10 phosphorylation involved in chromosome condensation Source: Ensembl
  • in utero embryonic development Source: Ensembl
  • mitotic cell cycle Source: RGD
  • mitotic metaphase plate congression Source: Ensembl
  • negative regulation of gene expression Source: RGD
  • negative regulation of protein phosphorylation Source: RGD
  • oocyte maturation Source: RGD
  • positive regulation of attachment of spindle microtubules to kinetochore Source: Ensembl
  • positive regulation of cardiac muscle cell proliferation Source: RGD
  • positive regulation of fibroblast proliferation Source: Ensembl
  • positive regulation of histone phosphorylation Source: RGD
  • positive regulation of mitotic cell cycle Source: Ensembl
  • positive regulation of mRNA 3'-end processing Source: RGD
  • protein complex assembly Source: RGD
  • regulation of cell cycle Source: RGD
  • regulation of chromosome condensation Source: RGD
  • regulation of mitotic cell cycle spindle assembly checkpoint Source: Ensembl
  • response to DDT Source: RGD
  • response to drug Source: RGD
  • response to mechanical stimulus Source: RGD
  • response to toxic substance Source: RGD
  • spermatogenesis Source: RGD
  • tissue regeneration Source: RGD
  • ventricular cardiac muscle cell development Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Cyclin

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-RNO-113507. E2F-enabled inhibition of pre-replication complex formation.
R-RNO-174048. APC/C:Cdc20 mediated degradation of Cyclin B.
R-RNO-176408. Regulation of APC/C activators between G1/S and early anaphase.
R-RNO-176412. Phosphorylation of the APC/C.
R-RNO-176417. Phosphorylation of Emi1.
R-RNO-2299718. Condensation of Prophase Chromosomes.
R-RNO-2500257. Resolution of Sister Chromatid Cohesion.
R-RNO-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-RNO-2980767. Activation of NIMA Kinases NEK9, NEK6, NEK7.
R-RNO-3301854. Nuclear Pore Complex (NPC) Disassembly.
R-RNO-380320. Recruitment of NuMA to mitotic centrosomes.
R-RNO-4419969. Depolymerisation of the Nuclear Lamina.
R-RNO-6804114. TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest.
R-RNO-69273. Cyclin A/B1 associated events during G2/M transition.
R-RNO-69478. G2/M DNA replication checkpoint.
R-RNO-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
R-RNO-8852276. The role of GTSE1 in G2/M progression after G2 checkpoint.

Names & Taxonomyi

Protein namesi
Recommended name:
G2/mitotic-specific cyclin-B1
Gene namesi
Name:Ccnb1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi2291. Ccnb1.

Subcellular locationi

GO - Cellular componenti

  • centrosome Source: UniProtKB
  • condensed nuclear chromosome outer kinetochore Source: Ensembl
  • cytoplasm Source: RGD
  • membrane Source: Ensembl
  • nucleus Source: RGD
  • spindle pole Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2093.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 423423G2/mitotic-specific cyclin-B1PRO_0000080353Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei116 – 1161Phosphoserine; by CDK1By similarity
Modified residuei118 – 1181PhosphoserineBy similarity
Modified residuei123 – 1231Phosphoserine; by PLK1By similarity
Modified residuei137 – 1371PhosphoserineBy similarity
Modified residuei311 – 3111PhosphothreonineBy similarity

Post-translational modificationi

Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases (By similarity).By similarity
Phosphorylated by PLK1 at Ser-123 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-137 was also reported to be mediated by PLK1 but Ser-123 seems to be the primary phosphorylation site (By similarity).By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP30277.
PRIDEiP30277.

PTM databases

iPTMnetiP30277.
PhosphoSiteiP30277.

Expressioni

Developmental stagei

Accumulates steadily during G2 and is abruptly destroyed at mitosis.

Gene expression databases

BgeeiENSRNOG00000018635.
GenevisibleiP30277. RN.

Interactioni

Subunit structurei

Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase. Interacts with CCNF; interaction is required for nuclear localization. Interacts with CDK5RAP3.By similarity

GO - Molecular functioni

  • protein kinase binding Source: RGD

Protein-protein interaction databases

IntActiP30277. 1 interaction.
STRINGi10116.ENSRNOP00000025297.

Chemistry

BindingDBiP30277.

Structurei

3D structure databases

ProteinModelPortaliP30277.
SMRiP30277. Positions 157-416.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni159 – 1679Interaction with CDK2By similarity
Regioni248 – 2514Interaction with CDK2By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi50 – 8233Lys-richAdd
BLAST

Sequence similaritiesi

Belongs to the cyclin family. Cyclin AB subfamily.Curated

Phylogenomic databases

eggNOGiKOG0653. Eukaryota.
COG5024. LUCA.
GeneTreeiENSGT00760000118939.
HOGENOMiHOG000167672.
HOVERGENiHBG061650.
InParanoidiP30277.
KOiK05868.
OMAiHMTVKNK.
OrthoDBiEOG091G0E9B.
PhylomeDBiP30277.
TreeFamiTF101001.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR013763. Cyclin-like.
IPR004367. Cyclin_C-dom.
IPR006671. Cyclin_N.
[Graphical view]
PfamiPF02984. Cyclin_C. 1 hit.
PF00134. Cyclin_N. 1 hit.
[Graphical view]
SMARTiSM00385. CYCLIN. 2 hits.
SM01332. Cyclin_C. 1 hit.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS00292. CYCLINS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30277-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALRVTRNTK INTENKAKVS MAGAKRVPVA VAASKPLLRS RTALGDIGNK
60 70 80 90 100
VSEQSRIPLK KETKKLGSGT VTVKALPKPV DKVPVCEPEV ELDEPEPEPV
110 120 130 140 150
MEVKHSPEPI LVDTPSPSPM ETSGCAPAEE YLCQAFSDVI LAVSDVDADD
160 170 180 190 200
GGDPNLCSEY VKDIYAYLRQ LEEEQSVRPK YLLGREVTGN MRAILIDWLI
210 220 230 240 250
QVQMKFRLLQ ETMYMTVSII DRFMQDSCVP KKMLQLVGVT AMFIASKYEE
260 270 280 290 300
MYPPEIGDFA FVTNNTYTKH QIRQMEMKIL RVLNFSLGRP LPLHFLRRAS
310 320 330 340 350
KIGEVDVEQH TLAKYLMELS MLDYDMVHFA PSQIAAGAFC LALKILDNGE
360 370 380 390 400
WTPTLQHYLS HTEESLLPVM QHLAKNIVMV NRGLTKHMTI KNKYATSKHA
410 420
KISTLAQLNC TLVQNLSKAV TKA
Length:423
Mass (Da):47,391
Last modified:April 1, 1993 - v1
Checksum:i4223CF71F144B279
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60768 mRNA. Translation: CAA43178.1.
X64589 mRNA. Translation: CAA45877.1.
L11995 mRNA. Translation: AAC00032.1.
BC059113 mRNA. Translation: AAH59113.1.
PIRiS34226.
RefSeqiNP_741988.1. NM_171991.3.
UniGeneiRn.9232.

Genome annotation databases

EnsembliENSRNOT00000082846; ENSRNOP00000075114; ENSRNOG00000058539.
GeneIDi25203.
KEGGirno:25203.
UCSCiRGD:2291. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60768 mRNA. Translation: CAA43178.1.
X64589 mRNA. Translation: CAA45877.1.
L11995 mRNA. Translation: AAC00032.1.
BC059113 mRNA. Translation: AAH59113.1.
PIRiS34226.
RefSeqiNP_741988.1. NM_171991.3.
UniGeneiRn.9232.

3D structure databases

ProteinModelPortaliP30277.
SMRiP30277. Positions 157-416.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP30277. 1 interaction.
STRINGi10116.ENSRNOP00000025297.

Chemistry

BindingDBiP30277.
ChEMBLiCHEMBL2093.

PTM databases

iPTMnetiP30277.
PhosphoSiteiP30277.

Proteomic databases

PaxDbiP30277.
PRIDEiP30277.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000082846; ENSRNOP00000075114; ENSRNOG00000058539.
GeneIDi25203.
KEGGirno:25203.
UCSCiRGD:2291. rat.

Organism-specific databases

CTDi891.
RGDi2291. Ccnb1.

Phylogenomic databases

eggNOGiKOG0653. Eukaryota.
COG5024. LUCA.
GeneTreeiENSGT00760000118939.
HOGENOMiHOG000167672.
HOVERGENiHBG061650.
InParanoidiP30277.
KOiK05868.
OMAiHMTVKNK.
OrthoDBiEOG091G0E9B.
PhylomeDBiP30277.
TreeFamiTF101001.

Enzyme and pathway databases

ReactomeiR-RNO-113507. E2F-enabled inhibition of pre-replication complex formation.
R-RNO-174048. APC/C:Cdc20 mediated degradation of Cyclin B.
R-RNO-176408. Regulation of APC/C activators between G1/S and early anaphase.
R-RNO-176412. Phosphorylation of the APC/C.
R-RNO-176417. Phosphorylation of Emi1.
R-RNO-2299718. Condensation of Prophase Chromosomes.
R-RNO-2500257. Resolution of Sister Chromatid Cohesion.
R-RNO-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-RNO-2980767. Activation of NIMA Kinases NEK9, NEK6, NEK7.
R-RNO-3301854. Nuclear Pore Complex (NPC) Disassembly.
R-RNO-380320. Recruitment of NuMA to mitotic centrosomes.
R-RNO-4419969. Depolymerisation of the Nuclear Lamina.
R-RNO-6804114. TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest.
R-RNO-69273. Cyclin A/B1 associated events during G2/M transition.
R-RNO-69478. G2/M DNA replication checkpoint.
R-RNO-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
R-RNO-8852276. The role of GTSE1 in G2/M progression after G2 checkpoint.

Miscellaneous databases

PROiP30277.

Gene expression databases

BgeeiENSRNOG00000018635.
GenevisibleiP30277. RN.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiIPR013763. Cyclin-like.
IPR004367. Cyclin_C-dom.
IPR006671. Cyclin_N.
[Graphical view]
PfamiPF02984. Cyclin_C. 1 hit.
PF00134. Cyclin_N. 1 hit.
[Graphical view]
SMARTiSM00385. CYCLIN. 2 hits.
SM01332. Cyclin_C. 1 hit.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS00292. CYCLINS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCCNB1_RAT
AccessioniPrimary (citable) accession number: P30277
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: September 7, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.