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P30275

- KCRU_MOUSE

UniProt

P30275 - KCRU_MOUSE

Protein

Creatine kinase U-type, mitochondrial

Gene

Ckmt1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

    Catalytic activityi

    ATP + creatine = ADP + phosphocreatine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei225 – 2251ATPPROSITE-ProRule annotation
    Binding sitei270 – 2701ATPPROSITE-ProRule annotation
    Binding sitei326 – 3261ATPPROSITE-ProRule annotation
    Binding sitei369 – 3691ATPPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi162 – 1665ATPPROSITE-ProRule annotation
    Nucleotide bindingi354 – 3596ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. creatine kinase activity Source: UniProtKB-EC
    3. protein binding Source: IntAct

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Creatine kinase U-type, mitochondrial (EC:2.7.3.2)
    Alternative name(s):
    Acidic-type mitochondrial creatine kinase
    Short name:
    Mia-CK
    Ubiquitous mitochondrial creatine kinase
    Short name:
    U-MtCK
    Gene namesi
    Name:Ckmt1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:99441. Ckmt1.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial inner membrane Source: UniProtKB-SubCell
    2. mitochondrion Source: MGI

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3939MitochondrionBy similarityAdd
    BLAST
    Chaini40 – 418379Creatine kinase U-type, mitochondrialPRO_0000016591Add
    BLAST

    Proteomic databases

    MaxQBiP30275.
    PaxDbiP30275.
    PRIDEiP30275.

    PTM databases

    PhosphoSiteiP30275.

    Expressioni

    Gene expression databases

    ArrayExpressiP30275.
    BgeeiP30275.
    CleanExiMM_CKMT1.
    GenevestigatoriP30275.

    Interactioni

    Subunit structurei

    Exists as an octamer composed of four MTCK homodimers.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    LRRK2Q5S0072EBI-773103,EBI-5323863From a different organism.

    Protein-protein interaction databases

    IntActiP30275. 6 interactions.
    MINTiMINT-4099745.

    Structurei

    3D structure databases

    ProteinModelPortaliP30275.
    SMRiP30275. Positions 40-418.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini46 – 13287Phosphagen kinase N-terminalPROSITE-ProRule annotationAdd
    BLAST
    Domaini159 – 401243Phosphagen kinase C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni40 – 6425Cardiolipin-bindingBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ATP:guanido phosphotransferase family.PROSITE-ProRule annotation
    Contains 1 phosphagen kinase C-terminal domain.PROSITE-ProRule annotation
    Contains 1 phosphagen kinase N-terminal domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG3869.
    HOGENOMiHOG000232165.
    HOVERGENiHBG001339.
    InParanoidiP30275.
    KOiK00933.
    OMAiERHNGYN.
    PhylomeDBiP30275.
    TreeFamiTF314214.

    Family and domain databases

    Gene3Di1.10.135.10. 1 hit.
    3.30.590.10. 1 hit.
    InterProiIPR022415. ATP-guanido_PTrfase_AS.
    IPR022414. ATP-guanido_PTrfase_cat.
    IPR022413. ATP-guanido_PTrfase_N.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    [Graphical view]
    PfamiPF00217. ATP-gua_Ptrans. 1 hit.
    PF02807. ATP-gua_PtransN. 1 hit.
    [Graphical view]
    SUPFAMiSSF48034. SSF48034. 1 hit.
    PROSITEiPS00112. PHOSPHAGEN_KINASE. 1 hit.
    PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
    PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P30275-1 [UniParc]FASTAAdd to Basket

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    MAGPFSRLLS ARPGLRLLAL AGAGSLTAGI LLRPESVGAA AAERRRLYPP    50
    SAEYPDLRKH NNCMASHLTP AVYARLCDKT TPTGWTLDQC IQTGVDNPGH 100
    PFIKTVGMVA GDEETYEVFA ELFDPVIQER HNGYDPRTMK HTTDLDASKI 150
    RSGYFDERYV LSSRVRTGRS IRGLSLPPAC TRAERREVER VVVDALSGLK 200
    GDLAGRYYRL SEMTEAEQQQ LIDDHFLFDK PVSPLLTAAG MARDWPDARG 250
    IWHNNEKSFL IWVNEEDHTR VISMEKGGNM KRVFERFCRG LKEVEKLIQE 300
    RGWEFMWNER LGYILTCPSN LGTGLRAGVH IKLPLLSKDN RFPKILENLR 350
    LQKRGTGGVD TAATGSVFDI SNLDRLGKSE VELVQLVIDG VNYLIDCERR 400
    LERGQDIRIP PPLVHSKH 418
    Length:418
    Mass (Da):47,004
    Last modified:April 1, 1993 - v1
    Checksum:i993CD8C290C8BFB9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z13968 mRNA. Translation: CAA78371.1.
    Z13969 Genomic DNA. Translation: CAA78372.1.
    BC025976 mRNA. Translation: AAH25976.1.
    CCDSiCCDS16640.1.
    PIRiI48308. S24612.
    RefSeqiNP_034027.1. NM_009897.2.
    XP_006498718.1. XM_006498655.1.
    XP_006498719.1. XM_006498656.1.
    UniGeneiMm.252145.

    Genome annotation databases

    EnsembliENSMUST00000000317; ENSMUSP00000000317; ENSMUSG00000000308.
    ENSMUST00000078222; ENSMUSP00000077349; ENSMUSG00000000308.
    GeneIDi12716.
    KEGGimmu:12716.
    UCSCiuc008lyt.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z13968 mRNA. Translation: CAA78371.1 .
    Z13969 Genomic DNA. Translation: CAA78372.1 .
    BC025976 mRNA. Translation: AAH25976.1 .
    CCDSi CCDS16640.1.
    PIRi I48308. S24612.
    RefSeqi NP_034027.1. NM_009897.2.
    XP_006498718.1. XM_006498655.1.
    XP_006498719.1. XM_006498656.1.
    UniGenei Mm.252145.

    3D structure databases

    ProteinModelPortali P30275.
    SMRi P30275. Positions 40-418.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P30275. 6 interactions.
    MINTi MINT-4099745.

    PTM databases

    PhosphoSitei P30275.

    Proteomic databases

    MaxQBi P30275.
    PaxDbi P30275.
    PRIDEi P30275.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000000317 ; ENSMUSP00000000317 ; ENSMUSG00000000308 .
    ENSMUST00000078222 ; ENSMUSP00000077349 ; ENSMUSG00000000308 .
    GeneIDi 12716.
    KEGGi mmu:12716.
    UCSCi uc008lyt.1. mouse.

    Organism-specific databases

    CTDi 12716.
    MGIi MGI:99441. Ckmt1.

    Phylogenomic databases

    eggNOGi COG3869.
    HOGENOMi HOG000232165.
    HOVERGENi HBG001339.
    InParanoidi P30275.
    KOi K00933.
    OMAi ERHNGYN.
    PhylomeDBi P30275.
    TreeFami TF314214.

    Miscellaneous databases

    NextBioi 281988.
    PROi P30275.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P30275.
    Bgeei P30275.
    CleanExi MM_CKMT1.
    Genevestigatori P30275.

    Family and domain databases

    Gene3Di 1.10.135.10. 1 hit.
    3.30.590.10. 1 hit.
    InterProi IPR022415. ATP-guanido_PTrfase_AS.
    IPR022414. ATP-guanido_PTrfase_cat.
    IPR022413. ATP-guanido_PTrfase_N.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    [Graphical view ]
    Pfami PF00217. ATP-gua_Ptrans. 1 hit.
    PF02807. ATP-gua_PtransN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48034. SSF48034. 1 hit.
    PROSITEi PS00112. PHOSPHAGEN_KINASE. 1 hit.
    PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
    PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mouse ubiquitous mitochondrial creatine kinase: gene organization and consequences from inactivation in mouse embryonic stem cells."
      Steeghs K., Peters W., Brueckwilder M., Croes H., van Alewijk D., Wieringa B.
      DNA Cell Biol. 14:539-553(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Colon.
    3. Lubec G., Kang S.U., Klug S., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 47-75; 105-130; 152-158; 173-182; 191-200; 250-270; 302-326 AND 355-375, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6 and OF1.
      Tissue: Brain and Hippocampus.

    Entry informationi

    Entry nameiKCRU_MOUSE
    AccessioniPrimary (citable) accession number: P30275
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Mitochondrial creatine kinase binds cardiolipin.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3