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P30275

- KCRU_MOUSE

UniProt

P30275 - KCRU_MOUSE

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Protein

Creatine kinase U-type, mitochondrial

Gene

Ckmt1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Catalytic activityi

ATP + creatine = ADP + phosphocreatine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei225 – 2251ATPPROSITE-ProRule annotation
Binding sitei270 – 2701ATPPROSITE-ProRule annotation
Binding sitei326 – 3261ATPPROSITE-ProRule annotation
Binding sitei369 – 3691ATPPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi162 – 1665ATPPROSITE-ProRule annotation
Nucleotide bindingi354 – 3596ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. creatine kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. negative regulation of apoptotic process Source: ParkinsonsUK-UCL
  2. negative regulation of protein binding Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_239426. Creatine metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Creatine kinase U-type, mitochondrial (EC:2.7.3.2)
Alternative name(s):
Acidic-type mitochondrial creatine kinase
Short name:
Mia-CK
Ubiquitous mitochondrial creatine kinase
Short name:
U-MtCK
Gene namesi
Name:Ckmt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:99441. Ckmt1.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial inner membrane Source: UniProtKB-KW
  2. mitochondrion Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3939MitochondrionBy similarityAdd
BLAST
Chaini40 – 418379Creatine kinase U-type, mitochondrialPRO_0000016591Add
BLAST

Proteomic databases

MaxQBiP30275.
PaxDbiP30275.
PRIDEiP30275.

PTM databases

PhosphoSiteiP30275.

Expressioni

Gene expression databases

BgeeiP30275.
CleanExiMM_CKMT1.
ExpressionAtlasiP30275. baseline and differential.
GenevestigatoriP30275.

Interactioni

Subunit structurei

Exists as an octamer composed of four MTCK homodimers.

Binary interactionsi

WithEntry#Exp.IntActNotes
LRRK2Q5S0072EBI-773103,EBI-5323863From a different organism.

Protein-protein interaction databases

IntActiP30275. 6 interactions.
MINTiMINT-4099745.

Structurei

3D structure databases

ProteinModelPortaliP30275.
SMRiP30275. Positions 40-418.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini46 – 13287Phosphagen kinase N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini159 – 401243Phosphagen kinase C-terminalPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni40 – 6425Cardiolipin-bindingBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the ATP:guanido phosphotransferase family.PROSITE-ProRule annotation
Contains 1 phosphagen kinase C-terminal domain.PROSITE-ProRule annotation
Contains 1 phosphagen kinase N-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG3869.
HOGENOMiHOG000232165.
HOVERGENiHBG001339.
InParanoidiP30275.
KOiK00933.
OMAiERHNGYN.
PhylomeDBiP30275.
TreeFamiTF314214.

Family and domain databases

Gene3Di1.10.135.10. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view]
PfamiPF00217. ATP-gua_Ptrans. 1 hit.
PF02807. ATP-gua_PtransN. 1 hit.
[Graphical view]
SUPFAMiSSF48034. SSF48034. 1 hit.
PROSITEiPS00112. PHOSPHAGEN_KINASE. 1 hit.
PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30275-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAGPFSRLLS ARPGLRLLAL AGAGSLTAGI LLRPESVGAA AAERRRLYPP
60 70 80 90 100
SAEYPDLRKH NNCMASHLTP AVYARLCDKT TPTGWTLDQC IQTGVDNPGH
110 120 130 140 150
PFIKTVGMVA GDEETYEVFA ELFDPVIQER HNGYDPRTMK HTTDLDASKI
160 170 180 190 200
RSGYFDERYV LSSRVRTGRS IRGLSLPPAC TRAERREVER VVVDALSGLK
210 220 230 240 250
GDLAGRYYRL SEMTEAEQQQ LIDDHFLFDK PVSPLLTAAG MARDWPDARG
260 270 280 290 300
IWHNNEKSFL IWVNEEDHTR VISMEKGGNM KRVFERFCRG LKEVEKLIQE
310 320 330 340 350
RGWEFMWNER LGYILTCPSN LGTGLRAGVH IKLPLLSKDN RFPKILENLR
360 370 380 390 400
LQKRGTGGVD TAATGSVFDI SNLDRLGKSE VELVQLVIDG VNYLIDCERR
410
LERGQDIRIP PPLVHSKH
Length:418
Mass (Da):47,004
Last modified:April 1, 1993 - v1
Checksum:i993CD8C290C8BFB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z13968 mRNA. Translation: CAA78371.1.
Z13969 Genomic DNA. Translation: CAA78372.1.
BC025976 mRNA. Translation: AAH25976.1.
CCDSiCCDS16640.1.
PIRiI48308. S24612.
RefSeqiNP_034027.1. NM_009897.2.
XP_006498718.1. XM_006498655.1.
XP_006498719.1. XM_006498656.1.
UniGeneiMm.252145.

Genome annotation databases

EnsembliENSMUST00000000317; ENSMUSP00000000317; ENSMUSG00000000308.
ENSMUST00000078222; ENSMUSP00000077349; ENSMUSG00000000308.
GeneIDi12716.
KEGGimmu:12716.
UCSCiuc008lyt.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z13968 mRNA. Translation: CAA78371.1 .
Z13969 Genomic DNA. Translation: CAA78372.1 .
BC025976 mRNA. Translation: AAH25976.1 .
CCDSi CCDS16640.1.
PIRi I48308. S24612.
RefSeqi NP_034027.1. NM_009897.2.
XP_006498718.1. XM_006498655.1.
XP_006498719.1. XM_006498656.1.
UniGenei Mm.252145.

3D structure databases

ProteinModelPortali P30275.
SMRi P30275. Positions 40-418.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P30275. 6 interactions.
MINTi MINT-4099745.

PTM databases

PhosphoSitei P30275.

Proteomic databases

MaxQBi P30275.
PaxDbi P30275.
PRIDEi P30275.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000000317 ; ENSMUSP00000000317 ; ENSMUSG00000000308 .
ENSMUST00000078222 ; ENSMUSP00000077349 ; ENSMUSG00000000308 .
GeneIDi 12716.
KEGGi mmu:12716.
UCSCi uc008lyt.1. mouse.

Organism-specific databases

CTDi 12716.
MGIi MGI:99441. Ckmt1.

Phylogenomic databases

eggNOGi COG3869.
HOGENOMi HOG000232165.
HOVERGENi HBG001339.
InParanoidi P30275.
KOi K00933.
OMAi ERHNGYN.
PhylomeDBi P30275.
TreeFami TF314214.

Enzyme and pathway databases

Reactomei REACT_239426. Creatine metabolism.

Miscellaneous databases

NextBioi 281988.
PROi P30275.
SOURCEi Search...

Gene expression databases

Bgeei P30275.
CleanExi MM_CKMT1.
ExpressionAtlasi P30275. baseline and differential.
Genevestigatori P30275.

Family and domain databases

Gene3Di 1.10.135.10. 1 hit.
3.30.590.10. 1 hit.
InterProi IPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view ]
Pfami PF00217. ATP-gua_Ptrans. 1 hit.
PF02807. ATP-gua_PtransN. 1 hit.
[Graphical view ]
SUPFAMi SSF48034. SSF48034. 1 hit.
PROSITEi PS00112. PHOSPHAGEN_KINASE. 1 hit.
PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse ubiquitous mitochondrial creatine kinase: gene organization and consequences from inactivation in mouse embryonic stem cells."
    Steeghs K., Peters W., Brueckwilder M., Croes H., van Alewijk D., Wieringa B.
    DNA Cell Biol. 14:539-553(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Colon.
  3. Lubec G., Kang S.U., Klug S., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 47-75; 105-130; 152-158; 173-182; 191-200; 250-270; 302-326 AND 355-375, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6 and OF1.
    Tissue: Brain and Hippocampus.

Entry informationi

Entry nameiKCRU_MOUSE
AccessioniPrimary (citable) accession number: P30275
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: November 26, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Mitochondrial creatine kinase binds cardiolipin.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3