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Protein

Creatine kinase U-type, mitochondrial

Gene

Ckmt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Miscellaneous

Mitochondrial creatine kinase binds cardiolipin.

Catalytic activityi

ATP + creatine = ADP + phosphocreatine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei225ATPPROSITE-ProRule annotation1
Binding sitei270ATPPROSITE-ProRule annotation1
Binding sitei326ATPPROSITE-ProRule annotation1
Binding sitei369ATPPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi162 – 166ATPPROSITE-ProRule annotation5
Nucleotide bindingi354 – 359ATPPROSITE-ProRule annotation6

GO - Molecular functioni

GO - Biological processi

  • negative regulation of apoptotic process Source: ParkinsonsUK-UCL
  • negative regulation of protein binding Source: ParkinsonsUK-UCL

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-71288 Creatine metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Creatine kinase U-type, mitochondrial (EC:2.7.3.2)
Alternative name(s):
Acidic-type mitochondrial creatine kinase
Short name:
Mia-CK
Ubiquitous mitochondrial creatine kinase
Short name:
U-MtCK
Gene namesi
Name:Ckmt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:99441 Ckmt1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 39MitochondrionBy similarityAdd BLAST39
ChainiPRO_000001659140 – 418Creatine kinase U-type, mitochondrialAdd BLAST379

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei152PhosphoserineBy similarity1
Modified residuei197PhosphoserineBy similarity1
Modified residuei214PhosphothreonineBy similarity1
Modified residuei233PhosphoserineCombined sources1
Modified residuei356PhosphothreonineBy similarity1
Modified residuei366PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP30275
PeptideAtlasiP30275
PRIDEiP30275

PTM databases

iPTMnetiP30275
PhosphoSitePlusiP30275
SwissPalmiP30275

Expressioni

Gene expression databases

BgeeiENSMUSG00000000308
CleanExiMM_CKMT1
ExpressionAtlasiP30275 baseline and differential
GenevisibleiP30275 MM

Interactioni

Subunit structurei

Exists as an octamer composed of four MTCK homodimers.

Binary interactionsi

Show more details

Protein-protein interaction databases

DIPiDIP-32284N
IntActiP30275 7 interactors.
MINTiP30275
STRINGi10090.ENSMUSP00000000317

Structurei

3D structure databases

ProteinModelPortaliP30275
SMRiP30275
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini46 – 132Phosphagen kinase N-terminalPROSITE-ProRule annotationAdd BLAST87
Domaini159 – 401Phosphagen kinase C-terminalPROSITE-ProRule annotationAdd BLAST243

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni40 – 64Cardiolipin-bindingBy similarityAdd BLAST25

Sequence similaritiesi

Belongs to the ATP:guanido phosphotransferase family.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG3581 Eukaryota
COG3869 LUCA
GeneTreeiENSGT00550000074561
HOGENOMiHOG000232165
HOVERGENiHBG001339
InParanoidiP30275
OMAiERHNGYN
OrthoDBiEOG091G0HZ0
PhylomeDBiP30275
TreeFamiTF314214

Family and domain databases

Gene3Di1.10.135.101 hit
InterProiView protein in InterPro
IPR000749 ATP-guanido_PTrfase
IPR022415 ATP-guanido_PTrfase_AS
IPR022414 ATP-guanido_PTrfase_cat
IPR022413 ATP-guanido_PTrfase_N
IPR036802 ATP-guanido_PTrfase_N_sf
IPR014746 Gln_synth/guanido_kin_cat_dom
PANTHERiPTHR11547 PTHR11547, 1 hit
PfamiView protein in Pfam
PF00217 ATP-gua_Ptrans, 1 hit
PF02807 ATP-gua_PtransN, 1 hit
SUPFAMiSSF48034 SSF48034, 1 hit
SSF55931 SSF55931, 1 hit
PROSITEiView protein in PROSITE
PS00112 PHOSPHAGEN_KINASE, 1 hit
PS51510 PHOSPHAGEN_KINASE_C, 1 hit
PS51509 PHOSPHAGEN_KINASE_N, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30275-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGPFSRLLS ARPGLRLLAL AGAGSLTAGI LLRPESVGAA AAERRRLYPP
60 70 80 90 100
SAEYPDLRKH NNCMASHLTP AVYARLCDKT TPTGWTLDQC IQTGVDNPGH
110 120 130 140 150
PFIKTVGMVA GDEETYEVFA ELFDPVIQER HNGYDPRTMK HTTDLDASKI
160 170 180 190 200
RSGYFDERYV LSSRVRTGRS IRGLSLPPAC TRAERREVER VVVDALSGLK
210 220 230 240 250
GDLAGRYYRL SEMTEAEQQQ LIDDHFLFDK PVSPLLTAAG MARDWPDARG
260 270 280 290 300
IWHNNEKSFL IWVNEEDHTR VISMEKGGNM KRVFERFCRG LKEVEKLIQE
310 320 330 340 350
RGWEFMWNER LGYILTCPSN LGTGLRAGVH IKLPLLSKDN RFPKILENLR
360 370 380 390 400
LQKRGTGGVD TAATGSVFDI SNLDRLGKSE VELVQLVIDG VNYLIDCERR
410
LERGQDIRIP PPLVHSKH
Length:418
Mass (Da):47,004
Last modified:April 1, 1993 - v1
Checksum:i993CD8C290C8BFB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z13968 mRNA Translation: CAA78371.1
Z13969 Genomic DNA Translation: CAA78372.1
BC025976 mRNA Translation: AAH25976.1
CCDSiCCDS16640.1
PIRiI48308 S24612
RefSeqiNP_034027.1, NM_009897.2
XP_006498718.1, XM_006498655.1
XP_006498719.1, XM_006498656.2
UniGeneiMm.252145

Genome annotation databases

EnsembliENSMUST00000000317; ENSMUSP00000000317; ENSMUSG00000000308
ENSMUST00000078222; ENSMUSP00000077349; ENSMUSG00000000308
GeneIDi12716
UCSCiuc008lyt.1 mouse

Similar proteinsi

Entry informationi

Entry nameiKCRU_MOUSE
AccessioniPrimary (citable) accession number: P30275
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: March 28, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome