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Protein

Creatine kinase U-type, mitochondrial

Gene

Ckmt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Catalytic activityi

ATP + creatine = ADP + phosphocreatine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei225ATPPROSITE-ProRule annotation1
Binding sitei270ATPPROSITE-ProRule annotation1
Binding sitei326ATPPROSITE-ProRule annotation1
Binding sitei369ATPPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi162 – 166ATPPROSITE-ProRule annotation5
Nucleotide bindingi354 – 359ATPPROSITE-ProRule annotation6

GO - Molecular functioni

GO - Biological processi

  • negative regulation of apoptotic process Source: ParkinsonsUK-UCL
  • negative regulation of protein binding Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-71288. Creatine metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Creatine kinase U-type, mitochondrial (EC:2.7.3.2)
Alternative name(s):
Acidic-type mitochondrial creatine kinase
Short name:
Mia-CK
Ubiquitous mitochondrial creatine kinase
Short name:
U-MtCK
Gene namesi
Name:Ckmt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:99441. Ckmt1.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • mitochondrial inner membrane Source: UniProtKB-SubCell
  • mitochondrion Source: ParkinsonsUK-UCL
  • myelin sheath Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 39MitochondrionBy similarityAdd BLAST39
ChainiPRO_000001659140 – 418Creatine kinase U-type, mitochondrialAdd BLAST379

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei152PhosphoserineBy similarity1
Modified residuei197PhosphoserineBy similarity1
Modified residuei214PhosphothreonineBy similarity1
Modified residuei233PhosphoserineCombined sources1
Modified residuei356PhosphothreonineBy similarity1
Modified residuei366PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP30275.
PeptideAtlasiP30275.
PRIDEiP30275.

PTM databases

iPTMnetiP30275.
PhosphoSitePlusiP30275.
SwissPalmiP30275.

Expressioni

Gene expression databases

BgeeiENSMUSG00000000308.
CleanExiMM_CKMT1.
ExpressionAtlasiP30275. baseline and differential.
GenevisibleiP30275. MM.

Interactioni

Subunit structurei

Exists as an octamer composed of four MTCK homodimers.

Binary interactionsi

WithEntry#Exp.IntActNotes
LRRK2Q5S0072EBI-773103,EBI-5323863From a different organism.

Protein-protein interaction databases

DIPiDIP-32284N.
IntActiP30275. 6 interactors.
MINTiMINT-4099745.
STRINGi10090.ENSMUSP00000000317.

Structurei

3D structure databases

ProteinModelPortaliP30275.
SMRiP30275.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini46 – 132Phosphagen kinase N-terminalPROSITE-ProRule annotationAdd BLAST87
Domaini159 – 401Phosphagen kinase C-terminalPROSITE-ProRule annotationAdd BLAST243

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni40 – 64Cardiolipin-bindingBy similarityAdd BLAST25

Sequence similaritiesi

Belongs to the ATP:guanido phosphotransferase family.PROSITE-ProRule annotation
Contains 1 phosphagen kinase C-terminal domain.PROSITE-ProRule annotation
Contains 1 phosphagen kinase N-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG3581. Eukaryota.
COG3869. LUCA.
GeneTreeiENSGT00550000074561.
HOGENOMiHOG000232165.
HOVERGENiHBG001339.
InParanoidiP30275.
KOiK00933.
OMAiERHNGYN.
OrthoDBiEOG091G0HZ0.
PhylomeDBiP30275.
TreeFamiTF314214.

Family and domain databases

Gene3Di1.10.135.10. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR000749. ATP-guanido_PTrfase.
IPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view]
PANTHERiPTHR11547. PTHR11547. 1 hit.
PfamiPF00217. ATP-gua_Ptrans. 1 hit.
PF02807. ATP-gua_PtransN. 1 hit.
[Graphical view]
SUPFAMiSSF48034. SSF48034. 1 hit.
PROSITEiPS00112. PHOSPHAGEN_KINASE. 1 hit.
PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30275-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGPFSRLLS ARPGLRLLAL AGAGSLTAGI LLRPESVGAA AAERRRLYPP
60 70 80 90 100
SAEYPDLRKH NNCMASHLTP AVYARLCDKT TPTGWTLDQC IQTGVDNPGH
110 120 130 140 150
PFIKTVGMVA GDEETYEVFA ELFDPVIQER HNGYDPRTMK HTTDLDASKI
160 170 180 190 200
RSGYFDERYV LSSRVRTGRS IRGLSLPPAC TRAERREVER VVVDALSGLK
210 220 230 240 250
GDLAGRYYRL SEMTEAEQQQ LIDDHFLFDK PVSPLLTAAG MARDWPDARG
260 270 280 290 300
IWHNNEKSFL IWVNEEDHTR VISMEKGGNM KRVFERFCRG LKEVEKLIQE
310 320 330 340 350
RGWEFMWNER LGYILTCPSN LGTGLRAGVH IKLPLLSKDN RFPKILENLR
360 370 380 390 400
LQKRGTGGVD TAATGSVFDI SNLDRLGKSE VELVQLVIDG VNYLIDCERR
410
LERGQDIRIP PPLVHSKH
Length:418
Mass (Da):47,004
Last modified:April 1, 1993 - v1
Checksum:i993CD8C290C8BFB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z13968 mRNA. Translation: CAA78371.1.
Z13969 Genomic DNA. Translation: CAA78372.1.
BC025976 mRNA. Translation: AAH25976.1.
CCDSiCCDS16640.1.
PIRiI48308. S24612.
RefSeqiNP_034027.1. NM_009897.2.
XP_006498718.1. XM_006498655.1.
XP_006498719.1. XM_006498656.2.
UniGeneiMm.252145.

Genome annotation databases

EnsembliENSMUST00000000317; ENSMUSP00000000317; ENSMUSG00000000308.
ENSMUST00000078222; ENSMUSP00000077349; ENSMUSG00000000308.
GeneIDi12716.
KEGGimmu:12716.
UCSCiuc008lyt.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z13968 mRNA. Translation: CAA78371.1.
Z13969 Genomic DNA. Translation: CAA78372.1.
BC025976 mRNA. Translation: AAH25976.1.
CCDSiCCDS16640.1.
PIRiI48308. S24612.
RefSeqiNP_034027.1. NM_009897.2.
XP_006498718.1. XM_006498655.1.
XP_006498719.1. XM_006498656.2.
UniGeneiMm.252145.

3D structure databases

ProteinModelPortaliP30275.
SMRiP30275.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-32284N.
IntActiP30275. 6 interactors.
MINTiMINT-4099745.
STRINGi10090.ENSMUSP00000000317.

PTM databases

iPTMnetiP30275.
PhosphoSitePlusiP30275.
SwissPalmiP30275.

Proteomic databases

PaxDbiP30275.
PeptideAtlasiP30275.
PRIDEiP30275.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000000317; ENSMUSP00000000317; ENSMUSG00000000308.
ENSMUST00000078222; ENSMUSP00000077349; ENSMUSG00000000308.
GeneIDi12716.
KEGGimmu:12716.
UCSCiuc008lyt.1. mouse.

Organism-specific databases

CTDi12716.
MGIiMGI:99441. Ckmt1.

Phylogenomic databases

eggNOGiKOG3581. Eukaryota.
COG3869. LUCA.
GeneTreeiENSGT00550000074561.
HOGENOMiHOG000232165.
HOVERGENiHBG001339.
InParanoidiP30275.
KOiK00933.
OMAiERHNGYN.
OrthoDBiEOG091G0HZ0.
PhylomeDBiP30275.
TreeFamiTF314214.

Enzyme and pathway databases

ReactomeiR-MMU-71288. Creatine metabolism.

Miscellaneous databases

PROiP30275.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000000308.
CleanExiMM_CKMT1.
ExpressionAtlasiP30275. baseline and differential.
GenevisibleiP30275. MM.

Family and domain databases

Gene3Di1.10.135.10. 1 hit.
3.30.590.10. 1 hit.
InterProiIPR000749. ATP-guanido_PTrfase.
IPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view]
PANTHERiPTHR11547. PTHR11547. 1 hit.
PfamiPF00217. ATP-gua_Ptrans. 1 hit.
PF02807. ATP-gua_PtransN. 1 hit.
[Graphical view]
SUPFAMiSSF48034. SSF48034. 1 hit.
PROSITEiPS00112. PHOSPHAGEN_KINASE. 1 hit.
PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKCRU_MOUSE
AccessioniPrimary (citable) accession number: P30275
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: November 2, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Mitochondrial creatine kinase binds cardiolipin.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.