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P30275 (KCRU_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Creatine kinase U-type, mitochondrial

EC=2.7.3.2
Alternative name(s):
Acidic-type mitochondrial creatine kinase
Short name=Mia-CK
Ubiquitous mitochondrial creatine kinase
Short name=U-MtCK
Gene names
Name:Ckmt1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length418 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Catalytic activity

ATP + creatine = ADP + phosphocreatine.

Subunit structure

Exists as an octamer composed of four MTCK homodimers.

Subcellular location

Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side.

Miscellaneous

Mitochondrial creatine kinase binds cardiolipin.

Sequence similarities

Belongs to the ATP:guanido phosphotransferase family.

Contains 1 phosphagen kinase C-terminal domain.

Contains 1 phosphagen kinase N-terminal domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

LRRK2Q5S0072EBI-773103,EBI-5323863From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3939Mitochondrion By similarity
Chain40 – 418379Creatine kinase U-type, mitochondrial
PRO_0000016591

Regions

Domain46 – 13287Phosphagen kinase N-terminal
Domain159 – 401243Phosphagen kinase C-terminal
Nucleotide binding162 – 1665ATP By similarity
Nucleotide binding354 – 3596ATP By similarity
Region40 – 6425Cardiolipin-binding By similarity

Sites

Binding site2251ATP By similarity
Binding site2701ATP By similarity
Binding site3261ATP By similarity
Binding site3691ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P30275 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 993CD8C290C8BFB9

FASTA41847,004
        10         20         30         40         50         60 
MAGPFSRLLS ARPGLRLLAL AGAGSLTAGI LLRPESVGAA AAERRRLYPP SAEYPDLRKH 

        70         80         90        100        110        120 
NNCMASHLTP AVYARLCDKT TPTGWTLDQC IQTGVDNPGH PFIKTVGMVA GDEETYEVFA 

       130        140        150        160        170        180 
ELFDPVIQER HNGYDPRTMK HTTDLDASKI RSGYFDERYV LSSRVRTGRS IRGLSLPPAC 

       190        200        210        220        230        240 
TRAERREVER VVVDALSGLK GDLAGRYYRL SEMTEAEQQQ LIDDHFLFDK PVSPLLTAAG 

       250        260        270        280        290        300 
MARDWPDARG IWHNNEKSFL IWVNEEDHTR VISMEKGGNM KRVFERFCRG LKEVEKLIQE 

       310        320        330        340        350        360 
RGWEFMWNER LGYILTCPSN LGTGLRAGVH IKLPLLSKDN RFPKILENLR LQKRGTGGVD 

       370        380        390        400        410 
TAATGSVFDI SNLDRLGKSE VELVQLVIDG VNYLIDCERR LERGQDIRIP PPLVHSKH 

« Hide

References

« Hide 'large scale' references
[1]"Mouse ubiquitous mitochondrial creatine kinase: gene organization and consequences from inactivation in mouse embryonic stem cells."
Steeghs K., Peters W., Brueckwilder M., Croes H., van Alewijk D., Wieringa B.
DNA Cell Biol. 14:539-553(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Colon.
[3]Lubec G., Kang S.U., Klug S., Sunyer B., Chen W.-Q.
Submitted (JAN-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 47-75; 105-130; 152-158; 173-182; 191-200; 250-270; 302-326 AND 355-375, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6 and OF1.
Tissue: Brain and Hippocampus.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z13968 mRNA. Translation: CAA78371.1.
Z13969 Genomic DNA. Translation: CAA78372.1.
BC025976 mRNA. Translation: AAH25976.1.
CCDSCCDS16640.1.
PIRS24612. I48308.
RefSeqNP_034027.1. NM_009897.2.
XP_006498718.1. XM_006498655.1.
XP_006498719.1. XM_006498656.1.
UniGeneMm.252145.

3D structure databases

ProteinModelPortalP30275.
SMRP30275. Positions 40-418.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP30275. 6 interactions.
MINTMINT-4099745.

PTM databases

PhosphoSiteP30275.

Proteomic databases

MaxQBP30275.
PaxDbP30275.
PRIDEP30275.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000000317; ENSMUSP00000000317; ENSMUSG00000000308.
ENSMUST00000078222; ENSMUSP00000077349; ENSMUSG00000000308.
GeneID12716.
KEGGmmu:12716.
UCSCuc008lyt.1. mouse.

Organism-specific databases

CTD12716.
MGIMGI:99441. Ckmt1.

Phylogenomic databases

eggNOGCOG3869.
HOGENOMHOG000232165.
HOVERGENHBG001339.
InParanoidP30275.
KOK00933.
OMAERHNGYN.
PhylomeDBP30275.
TreeFamTF314214.

Gene expression databases

ArrayExpressP30275.
BgeeP30275.
CleanExMM_CKMT1.
GenevestigatorP30275.

Family and domain databases

Gene3D1.10.135.10. 1 hit.
3.30.590.10. 1 hit.
InterProIPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view]
PfamPF00217. ATP-gua_Ptrans. 1 hit.
PF02807. ATP-gua_PtransN. 1 hit.
[Graphical view]
SUPFAMSSF48034. SSF48034. 1 hit.
PROSITEPS00112. PHOSPHAGEN_KINASE. 1 hit.
PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio281988.
PROP30275.
SOURCESearch...

Entry information

Entry nameKCRU_MOUSE
AccessionPrimary (citable) accession number: P30275
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: July 9, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot