Reviewed, UniProtKB/Swiss-Prot P30271 (AMYB_SECCE)

Names and origin

Protein names

Recommended name:
    Beta-amylase
    EC=3.2.1.2
Alternative name(s):
    1,4-alpha-D-glucan maltohydrolase

Gene names

Name:

BMY1

Organism

Secale cereale (Rye)

Taxonomic identifier

4550 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Secale

Protein attributes

Sequence length	222 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains.
Sequence similarities	Belongs to the glycosyl hydrolase 14 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Polysaccharide degradation
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-amylase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 222	›222	Beta-amylase		PRO_0000153937
Sites
	Active site	74	1	By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P30271-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 29A66E6EA5C0F718	FASTA	222	24,349
	10 20 30 40 50 60 SHAAEVTAGY YNLHDRDDYR PIARMLTRHH ASLNFTCAEM RDSEQSSQAM SAPEELVQQV 70 80 90 100 110 120 WSAGWREGLN IACENALPRY DPTAYNTILR NARPHGINHS SPTEHKLFGF TYLRLSNQLL 130 140 150 160 170 180 EGQNYVNFKT FVDRMHANLP HDPSVDPVAP LQRSGPEIPI EVILQAAQPK LDPFPFEDHT 190 200 210 220 DLPVQCLGGI GGGEVECPAG GIGGEVQQDP TGGMGGELPP AV

References

[1]

"Characterization of cDNA clones for rye endosperm beta-amylase and analysis of beta-amylase deficiency in rye mutant lines." Rorat T., Sadowski J., Grellet F., Daussant J., Delseny M. Theor. Appl. Genet. 83:257-263(1991) [Agricola: IND92007634] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Endosperm.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X56785 mRNA. Translation: CAA40105.1.
PIR	S38779.
3D structure databases
HSSP	HSSP built from PDB template 1B1Y based on UniProtKB P16098.
SMR	P30271. Positions 1-200.
ModBase	Search...
Protein family/group databases
CAZy	GH14. Glycoside Hydrolase Family 14.
Organism-specific databases
Gramene	P30271.
Enzyme and pathway databases
BRENDA	3.2.1.2. 1472.
Family and domain databases
InterPro	IPR001554. Glyco_hydro_14. IPR018238. Glyco_hydro_14_CS. IPR001371. Glyco_hydro_14B_pln. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF01373. Glyco_hydro_14. 1 hit. [Graphical view]
PRINTS	PR00750. BETAAMYLASE. PR00842. GLHYDLASE14B.
PROSITE	PS00506. BETA_AMYLASE_1. Partial match. PS00679. BETA_AMYLASE_2. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMYB_SECCE

Accession

Primary (citable) accession number: P30271

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)