Reviewed, UniProtKB/Swiss-Prot P30261 (DPOD3_SCHPO)

Names and origin

Protein names

Recommended name:
    DNA polymerase delta subunit 3
Alternative name(s):
    Cell division control protein 27

Gene names

Name:	cdc27
ORF Names:	SPBC1734.02c, SPBC337.18c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subunit structure	Heterotetramer that consist of the pol3, cdc1, cdc27 and cdm1 subunits. Cdc27 interacts with cdc1 and is required for dimerization of the tetramer. Ref.3
Subcellular location	Nucleus By similarity.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA replication Mitosis
Cellular component	Nucleus
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication, removal of RNA primer Traceable author statement. Source: GeneDB_SPombe DNA synthesis during DNA repair Traceable author statement. Source: GeneDB_SPombe cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW telomere maintenance Inferred from mutant phenotype. Source: GeneDB_SPombe
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_SPombe delta DNA polymerase complex Traceable author statement. Source: GeneDB_SPombe
Molecular function	DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
cdc1	P87324	1	EBI-866919,EBI-865227
pcn1	Q03392	3	EBI-866919,EBI-768724

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	DNA polymerase delta subunit 3		PRO_0000186049
Amino acid modifications
	Modified residue	163	1	Phosphoserine Ref.4
Experimental info
	Mutagenesis	57	1	G → E: Cell cycle arrest at 35 degrees Celsius.
	Mutagenesis	57	1	G → R: Cell cycle arrest at 35 degrees Celsius.

Sequences

	Sequence		Length	Mass (Da)
	P30261-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 6E6F9AD407B17673	FASTA	372	42,350
	10 20 30 40 50 60 MEEWRNFLDI KVINESSLVT VDNLSLQLDI SSEKAQEYLN MFYQGNDFLY PIYLIHGQPI 70 80 90 100 110 120 DDEINLEIDE ESQPISNFPV LQYILCDKSS LQEKQSRLKS GYKTVIFALS SAPLSDFDEL 130 140 150 160 170 180 LPAVYEIREK DVLYKKEDAD KYGFIFNENS VPRVLKKAPS THSPQLSVPS KTSTIDKTDT 190 200 210 220 230 240 RSTEKTKGKD IFSNARNQKG NSSRKNKKAP LENHKEKEPL LPKEEKLSEQ AKRERDDLKN 250 260 270 280 290 300 IMQLEDESVS TTSVHDSEDD NLDSNNFQLE IGTEAKSAAP DEPQEIIKSV SGGKRRGKRK 310 320 330 340 350 360 VKKYATTKDE EGFLVTKEEE VWESFSEDEN ISTGTSNVVR NKPTTVNIAT KKKNTAQSKP 370 QQKSIMSFFG KK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and sequence analysis of cdc27+ required for the G2-M transition in the fission yeast Schizosaccharomyces pombe." Hughes D.A., Macneill S.A., Fantes P.A. Mol. Gen. Genet. 231:401-410(1992) [PubMed: 1538696] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[3]	"Structure and activity associated with multiple forms of Schizosaccharomyces pombe DNA polymerase delta." Zuo S., Bermudez V., Zhang G., Kelman Z., Hurwitz J. J. Biol. Chem. 275:5153-5162(2000) [PubMed: 10671561] [Abstract] Cited for: FUNCTION, SUBUNIT.
[4]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed: 18257517] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-163, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M74062 Genomic DNA. Translation: AAA35295.1. M83307 mRNA. Translation: AAA35296.1. CU329671 Genomic DNA. Translation: CAA21288.1.
PIR	T39649.
RefSeq	NP_595419.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P30261. 2 interactions.
STRING	P30261.
Genome annotation databases
GeneID	2539627.
GenomeReviews	Gene locus cdc27 in contig CU329671_GR.
KEGG	spo:SPBC1734.02c.
NMPDR	fig|4896.1.peg.1285.
Organism-specific databases
GeneDB_Spombe	SPBC1734.02c.
Gene expression databases
ArrayExpress	P30261.
Family and domain databases
InterPro	IPR019038. DNA_polymerase_subunit_Cdc27. [Graphical view]
Pfam	PF09507. CDC27. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DPOD3_SCHPO

Accession

Primary (citable) accession number: P30261

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	November 24, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)