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Protein

Pseudouridine kinase

Gene

psuK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of pseudouridine to pseudouridine 5'-phosphate (PsiMP).1 Publication

Catalytic activityi

ATP + pseudouridine = ADP + pseudouridine 5'-phosphate.1 Publication

Kineticsi

  1. KM=120 µM for pseudouridine (in the presence of 0.5 mM ATP)1 Publication
  2. KM=330 µM for ATP (in the presence of 200 µM pseudouridine)1 Publication

    GO - Molecular functioni

    • ATP binding Source: UniProtKB-KW
    • pseudouridine kinase activity Source: EcoliWiki
    Complete GO annotation...

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11646-MONOMER.
    ECOL316407:JW2153-MONOMER.
    BRENDAi2.7.1.83. 2026.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pseudouridine kinase (EC:2.7.1.83)
    Gene namesi
    Name:psuK
    Synonyms:pscK, yeiC
    Ordered Locus Names:b2166, JW2153
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG11646. psuK.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000801471 – 313Pseudouridine kinaseAdd BLAST313

    Proteomic databases

    PaxDbiP30235.
    PRIDEiP30235.

    Interactioni

    Protein-protein interaction databases

    BioGridi4260461. 17 interactors.
    IntActiP30235. 11 interactors.
    STRINGi511145.b2166.

    Structurei

    3D structure databases

    ProteinModelPortaliP30235.
    SMRiP30235.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the carbohydrate kinase PfkB family.Curated

    Phylogenomic databases

    eggNOGiENOG4105DD7. Bacteria.
    COG0524. LUCA.
    HOGENOMiHOG000064422.
    InParanoidiP30235.
    KOiK16328.
    OMAiFAQGCSA.
    PhylomeDBiP30235.

    Family and domain databases

    Gene3Di3.40.1190.20. 1 hit.
    InterProiIPR002173. Carboh/pur_kinase_PfkB_CS.
    IPR011611. PfkB_dom.
    IPR029056. Ribokinase-like.
    [Graphical view]
    PfamiPF00294. PfkB. 1 hit.
    [Graphical view]
    SUPFAMiSSF53613. SSF53613. 1 hit.
    PROSITEiPS00583. PFKB_KINASES_1. 1 hit.
    PS00584. PFKB_KINASES_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P30235-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MREKDYVVII GSANIDVAGY SHESLNYADS NPGKIKFTPG GVGRNIAQNL
    60 70 80 90 100
    ALLGNKAWLL SAVGSDFYGQ SLLTQTNQSG VYVDKCLIVP GENTSSYLSL
    110 120 130 140 150
    LDNTGEMLVA INDMNISNAI TAEYLAQHGE FIQRAKVIVA DCNISEEALA
    160 170 180 190 200
    WILDNAANVP VFVDPVSAWK CVKVRDRLNQ IHTLKPNRLE AETLSGIALS
    210 220 230 240 250
    GREDVAKVAA WFHQHGLNRL VLSMGGDGVY YSDISGESGW SAPIKTNVIN
    260 270 280 290 300
    VTGAGDAMMA GLASCWVDGM PFAESVRFAQ GCSSMALSCE YTNNPDLSIA
    310
    NVISLVENAE CLN
    Length:313
    Mass (Da):33,573
    Last modified:October 1, 1993 - v2
    Checksum:i89D0F8439D148103
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00007 Genomic DNA. Translation: AAA60507.1.
    U00096 Genomic DNA. Translation: AAC75227.1.
    AP009048 Genomic DNA. Translation: BAE76643.1.
    M23196 Genomic DNA. Translation: AAA62625.1.
    PIRiE64985.
    RefSeqiNP_416671.1. NC_000913.3.
    WP_001208118.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75227; AAC75227; b2166.
    BAE76643; BAE76643; BAE76643.
    GeneIDi946664.
    KEGGiecj:JW2153.
    eco:b2166.
    PATRICi32119681. VBIEscCol129921_2251.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00007 Genomic DNA. Translation: AAA60507.1.
    U00096 Genomic DNA. Translation: AAC75227.1.
    AP009048 Genomic DNA. Translation: BAE76643.1.
    M23196 Genomic DNA. Translation: AAA62625.1.
    PIRiE64985.
    RefSeqiNP_416671.1. NC_000913.3.
    WP_001208118.1. NZ_LN832404.1.

    3D structure databases

    ProteinModelPortaliP30235.
    SMRiP30235.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4260461. 17 interactors.
    IntActiP30235. 11 interactors.
    STRINGi511145.b2166.

    Proteomic databases

    PaxDbiP30235.
    PRIDEiP30235.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC75227; AAC75227; b2166.
    BAE76643; BAE76643; BAE76643.
    GeneIDi946664.
    KEGGiecj:JW2153.
    eco:b2166.
    PATRICi32119681. VBIEscCol129921_2251.

    Organism-specific databases

    EchoBASEiEB1599.
    EcoGeneiEG11646. psuK.

    Phylogenomic databases

    eggNOGiENOG4105DD7. Bacteria.
    COG0524. LUCA.
    HOGENOMiHOG000064422.
    InParanoidiP30235.
    KOiK16328.
    OMAiFAQGCSA.
    PhylomeDBiP30235.

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11646-MONOMER.
    ECOL316407:JW2153-MONOMER.
    BRENDAi2.7.1.83. 2026.

    Miscellaneous databases

    PROiP30235.

    Family and domain databases

    Gene3Di3.40.1190.20. 1 hit.
    InterProiIPR002173. Carboh/pur_kinase_PfkB_CS.
    IPR011611. PfkB_dom.
    IPR029056. Ribokinase-like.
    [Graphical view]
    PfamiPF00294. PfkB. 1 hit.
    [Graphical view]
    SUPFAMiSSF53613. SSF53613. 1 hit.
    PROSITEiPS00583. PFKB_KINASES_1. 1 hit.
    PS00584. PFKB_KINASES_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPSUK_ECOLI
    AccessioniPrimary (citable) accession number: P30235
    Secondary accession number(s): Q2MAR3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: October 1, 1993
    Last modified: November 2, 2016
    This is version 108 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.