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Reviewed, UniProtKB/Swiss-Prot P30234 (DHA_MYCTU)

Last modified June 16, 2009. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Alanine dehydrogenase
    EC=1.4.1.1
Alternative name(s):
    40 kDa antigen
    TB43
Gene names
Name: ald
Ordered Locus Names: Rv2780, MT2850
ORF Names: MTV002.45
OrganismMycobacterium tuberculosis [Complete proteome] [HAMAP]
Taxonomic identifier1773 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

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Protein attributes

Sequence length371 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

May play a role in cell wall synthesis as L-alanine is an important constituent of the peptidoglycan layer.

Catalytic activity

L-alanine + H2O + NAD+ = pyruvate + NH3 + NADH.

Pathway

Amino-acid degradation; L-alanine degradation via dehydrogenase pathway; NH(3) and pyruvate from L-alanine: step 1/1.

Subcellular location

Secreted.

Sequence similarities

Belongs to the AlaDH/PNT family.

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Ontologies

Keywords
   Cellular componentSecreted
   LigandNAD
   Molecular functionOxidoreductase
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological processoxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionalanine dehydrogenase activity

Inferred from electronic annotation. Source: EC

binding

Inferred from electronic annotation. Source: InterPro

electron carrier activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 371371Alanine dehydrogenase
PRO_0000198994

Regions

Nucleotide binding170 – 20031NAD By similarity

Sites

Active site961 Potential

Experimental info

Sequence conflict131E → EFQ in CAA44791. Ref.1
Sequence conflict1231A → P in AAK47169. Ref.4

Secondary structure

1 ...................................................................... 371
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P30234-1 [UniParc].

Last modified July 15, 1999. Version 2.
Checksum: 9DF7540524DC116A

FASTA37138,713
        10         20         30         40         50         60 
MRVGIPTETK NNEFRVAITP AGVAELTRRG HEVLIQAGAG EGSAITDADF KAAGAQLVGT 

        70         80         90        100        110        120 
ADQVWADADL LLKVKEPIAA EYGRLRHGQI LFTFLHLAAS RACTDALLDS GTTSIAYETV 

       130        140        150        160        170        180 
QTADGALPLL APMSEVAGRL AAQVGAYHLM RTQGGRGVLM GGVPGVEPAD VVVIGAGTAG 

       190        200        210        220        230        240 
YNAARIANGM GATVTVLDIN IDKLRQLDAE FCGRIHTRYS SAYELEGAVK RADLVIGAVL 

       250        260        270        280        290        300 
VPGAKAPKLV SNSLVAHMKP GAVLVDIAID QGGCFEGSRP TTYDHPTFAV HDTLFYCVAN 

       310        320        330        340        350        360 
MPASVPKTST YALTNATMPY VLELADHGWR AACRSNPALA KGLSTHEGAL LSERVATDLG 

       370 
VPFTEPASVL A

« Hide

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References

« Hide 'large scale' references
[1]"Structure and function of a 40,000-molecular-weight protein antigen of Mycobacterium tuberculosis."
Andersen A.B., Andersen P., Ljungqvist L.
Infect. Immun. 60:2317-2323(1992) [PubMed: 1587598] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 35801 / TMC 107 / Erdman.
[2]"Host-vector system for high level expression and purification of enzymatically active L-alanine dehydrogenase of M.tuberculosis in E.coli."
Singh M., Hutter B.
Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 25618 / H37Rv.
[3]"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence."
Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. expand/collapse author list , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G.
Nature 393:537-544(1998) [PubMed: 9634230] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25618 / H37Rv.
[4]"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains."
Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. expand/collapse author list , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.
J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CDC 1551 / Oshkosh.
[5]"Isolation of a 43 kDa protein from Mycobacterium tuberculosis H37Rv and its identification as a pyridine nucleotide transhydrogenase."
Deshpande R.G., Khan M.B., Bhat D.A., Navalkar R.G.
J. Appl. Bacteriol. 77:639-643(1994) [PubMed: 7822223] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-21.
Strain: ATCC 25618 / H37Rv.
+Additional computationally mapped references.

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Cross-references

Sequence databases

X63069 Genomic DNA. Translation: CAA44791.1.
U92472 Genomic DNA. Translation: AAC38804.1.
BX842580 Genomic DNA. Translation: CAA15575.1.
AE000516 Genomic DNA. Translation: AAK47169.1.
PIRA43830. C70883.
RefSeqNP_217296.1.
NP_337355.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2VHVX-ray2.80A/B1-371[»]
2VHWX-ray2.00A/B/C/D/E/F1-371[»]
2VHXX-ray2.00A/B/C/D/E/F1-371[»]
2VHYX-ray2.30A/B1-371[»]
2VHZX-ray2.04A/B1-371[»]
2VOEX-ray2.60A/B/C/D/E/F1-371[»]
2VOJX-ray2.60A/C/E1-371[»]
ModBaseSearch...

Genome annotation databases

GeneID888493.
925435.
GenomeReviewsGene locus MT2850 in contig AE000516_GR.
Gene locus Rv2780 in contig AL123456_GR.
KEGGmtc:MT2850.
mtu:Rv2780.
TIGRMT2850.

Organism-specific databases

TubercuListRv2780.

Phylogenomic databases

HOGENOMP30234.
OMAP30234. QNAANIA.

Enzyme and pathway databases

BRENDA1.4.1.1. 809.

Family and domain databases

InterProIPR007698. Ala_DH/PNT_C.
IPR008142. Ala_DH/PNT_CS1.
IPR008143. Ala_DH/PNT_CS2.
IPR007886. Ala_DH/PNT_N.
IPR008141. Ala_DH_PNT.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PfamPF01262. AlaDh_PNT_C. 1 hit.
PF05222. AlaDh_PNT_N. 1 hit.
[Graphical view]
ProDomPD000139. FAD_pyr_redox. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR00518. alaDH. 1 hit.
PROSITEPS00836. ALADH_PNT_1. 1 hit.
PS00837. ALADH_PNT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameDHA_MYCTU
AccessionPrimary (citable) accession number: P30234
Secondary accession number(s): O33322
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: July 15, 1999
Last modified: June 16, 2009
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents