Reviewed, UniProtKB/Swiss-Prot P30234 (DHA_MYCTU)

Names and origin

Protein names

Recommended name:
    Alanine dehydrogenase
    EC=1.4.1.1
Alternative name(s):
    40 kDa antigen
    TB43

Gene names

Name:	ald
Ordered Locus Names:	Rv2780, MT2850
ORF Names:	MTV002.45

Organism

Mycobacterium tuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	371 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May play a role in cell wall synthesis as L-alanine is an important constituent of the peptidoglycan layer.
Catalytic activity	L-alanine + H2O + NAD+ = pyruvate + NH3 + NADH.
Pathway	Amino-acid degradation; L-alanine degradation via dehydrogenase pathway; NH(3) and pyruvate from L-alanine: step 1/1.
Subcellular location	Secreted.
Sequence similarities	Belongs to the AlaDH/PNT family.

Ontologies

Keywords
Cellular component	Secreted
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alanine dehydrogenase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 371

371

Alanine dehydrogenase

PRO_0000198994

Regions

Nucleotide binding

170 – 200

31

NAD By similarity

Sites

Active site

96

1

Potential

Experimental info

Sequence conflict

13

1

E → EFQ in CAA44791. Ref.1

Sequence conflict

123

1

A → P in AAK47169. Ref.4

Secondary structure

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371

Helix Strand Turn

Details...

Beta strand

2 – 5

4

Helix

20 – 28

9

Beta strand

32 – 36

5

Turn

37 – 40

4

Helix

41 – 43

3

Helix

47 – 53

7

Beta strand

56 – 59

4

Helix

61 – 67

7

Beta strand

69 – 72

4

Helix

79 – 84

6

Beta strand

90 – 93

4

Helix

97 – 99

3

Helix

101 – 110

10

Beta strand

113 – 116

4

Helix

117 – 119

3

Turn

128 – 130

3

Helix

131 – 148

18

Helix

151 – 153

3

Beta strand

170 – 174

5

Helix

178 – 189

12

Beta strand

193 – 199

7

Helix

201 – 210

10

Turn

211 – 213

3

Beta strand

214 – 219

6

Helix

222 – 231

10

Beta strand

233 – 237

5

Helix

252 – 255

4

Beta strand

263 – 266

4

Helix

267 – 270

4

Beta strand

283 – 285

3

Beta strand

287 – 290

4

Beta strand

293 – 296

4

Helix

301 – 304

4

Helix

306 – 335

30

Helix

337 – 340

4

Beta strand

343 – 346

4

Helix

353 – 359

7

Helix

367 – 370

4

Sequences

	Sequence		Length	Mass (Da)
	P30234-1 [UniParc]. Last modified July 15, 1999. Version 2. Checksum: 9DF7540524DC116A	FASTA	371	38,713
	10 20 30 40 50 60 MRVGIPTETK NNEFRVAITP AGVAELTRRG HEVLIQAGAG EGSAITDADF KAAGAQLVGT 70 80 90 100 110 120 ADQVWADADL LLKVKEPIAA EYGRLRHGQI LFTFLHLAAS RACTDALLDS GTTSIAYETV 130 140 150 160 170 180 QTADGALPLL APMSEVAGRL AAQVGAYHLM RTQGGRGVLM GGVPGVEPAD VVVIGAGTAG 190 200 210 220 230 240 YNAARIANGM GATVTVLDIN IDKLRQLDAE FCGRIHTRYS SAYELEGAVK RADLVIGAVL 250 260 270 280 290 300 VPGAKAPKLV SNSLVAHMKP GAVLVDIAID QGGCFEGSRP TTYDHPTFAV HDTLFYCVAN 310 320 330 340 350 360 MPASVPKTST YALTNATMPY VLELADHGWR AACRSNPALA KGLSTHEGAL LSERVATDLG 370 VPFTEPASVL A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and function of a 40,000-molecular-weight protein antigen of Mycobacterium tuberculosis." Andersen A.B., Andersen P., Ljungqvist L. Infect. Immun. 60:2317-2323(1992) [PubMed: 1587598] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 35801 / TMC 107 / Erdman.
[2]	"Host-vector system for high level expression and purification of enzymatically active L-alanine dehydrogenase of M.tuberculosis in E.coli." Singh M., Hutter B. Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[3]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[4]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[5]	"Isolation of a 43 kDa protein from Mycobacterium tuberculosis H37Rv and its identification as a pyridine nucleotide transhydrogenase." Deshpande R.G., Khan M.B., Bhat D.A., Navalkar R.G. J. Appl. Bacteriol. 77:639-643(1994) [PubMed: 7822223] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-21. Strain: ATCC 25618 / H37Rv.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X63069 Genomic DNA. Translation: CAA44791.1.
U92472 Genomic DNA. Translation: AAC38804.1.
BX842580 Genomic DNA. Translation: CAA15575.1.
AE000516 Genomic DNA. Translation: AAK47169.1.

PIR

A43830. C70883.

RefSeq

NP_217296.1.
NP_337355.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2VHV	X-ray	2.80	A/B	1-371	[»]
2VHW	X-ray	2.00	A/B/C/D/E/F	1-371	[»]
2VHX	X-ray	2.00	A/B/C/D/E/F	1-371	[»]
2VHY	X-ray	2.30	A/B	1-371	[»]
2VHZ	X-ray	2.04	A/B	1-371	[»]
2VOE	X-ray	2.60	A/B/C/D/E/F	1-371	[»]
2VOJ	X-ray	2.60	A/C/E	1-371	[»]

ModBase

Search...

Genome annotation databases

GeneID

888493.
925435.

GenomeReviews

Gene locus MT2850 in contig AE000516_GR.

KEGG

mtc:MT2850.
mtu:Rv2780.

TIGR

MT2850.

Organism-specific databases

TubercuList

Rv2780.

Phylogenomic databases

HOGENOM

HBG289735.

OMA

GGLATHE.

Enzyme and pathway databases

BRENDA

1.4.1.1. 809.

Family and domain databases

InterPro

IPR007698. Ala_DH/PNT_C.
IPR008142. Ala_DH/PNT_CS1.
IPR008143. Ala_DH/PNT_CS2.
IPR007886. Ala_DH/PNT_N.
IPR008141. Ala_DH/Pyr_transHydgase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.

Pfam

PF01262. AlaDh_PNT_C. 1 hit.
PF05222. AlaDh_PNT_N. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00518. alaDH. 1 hit.

PROSITE

PS00836. ALADH_PNT_1. 1 hit.
PS00837. ALADH_PNT_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

DHA_MYCTU

Accession

Primary (citable) accession number: P30234
Secondary accession number(s): O33322

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	July 15, 1999
Last modified:	February 9, 2010
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)