Sweet protein mabinlin-2 precursor - Capparis masaikai (Mabinlang)

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Reviewed, UniProtKB/Swiss-Prot P30233 (2SS2_CAPMA)

Last modified November 24, 2009. Version 66. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names	Recommended name: Sweet protein mabinlin-2 Alternative name(s): Mabinlin II Short name=MAB II Cleaved into the following 2 chains: 1- Recommended name: Sweet protein mabinlin-2 chain A 2- Recommended name: Sweet protein mabinlin-2 chain B
Organism	Capparis masaikai (Mabinlang)
Taxonomic identifier	13395 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Capparaceae › Capparis

Protein attributes

Sequence length	155 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Heat stable 2S seed storage protein having sweetness-inducing activity.
Subunit structure	Heterodimer of a small A and a large B chain linked by disulfide bonds.
Sequence similarities	Belongs to the 2S seed storage albumins family.

Ontologies

Keywords
Domain	Signal
Molecular function	Seed storage protein Storage protein Taste-modifying protein
PTM	Disulfide bond Pyrrolidone carboxylic acid
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Molecular function	nutrient reservoir activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

20

Potential

Propeptide

15

PRO_0000032137

Chain

33

Sweet protein mabinlin-2 chain A

PRO_0000032138

Propeptide

14

PRO_0000032139

Chain

72

Sweet protein mabinlin-2 chain B

PRO_0000032140

Propeptide

1

PRO_0000032141

Amino acid modifications

Modified residue

1

Pyrrolidone carboxylic acid

Modified residue

1

Pyrrolidone carboxylic acid

Disulfide bond

Disulfide bond

Disulfide bond

Disulfide bond

Experimental info

Sequence conflict

1

A → T in BAA12204. Ref.1

Sequence conflict

1

A → T in BAA12204. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

155

Helix Strand Turn

Sequences

Sequence

Length

Mass (Da)

Tools

P30233-1 [UniParc].

Last modified February 21, 2001. Version 3.
Checksum: 72E885DEDCC2D46A
Show »

155

18,089

        10         20         30         40         50         60 
MAKLIFLFAT LALFVLLANA SIQTTVIEVD EEEDNQLWRC QRQFLQHQRL RACQRFIHRR 

        70         80         90        100        110        120 
AQFGGQPDEL EDEVEDDNDD ENQPRRPALR QCCNQLRQVD RPCVCPVLRQ AAQQVLQRQI 

       130        140        150 
IQGPQQLRRL FDAARNLPNI CNIPNIGACP FRAWP

References

[1]	"Cloning and sequencing of a cDNA encoding a heat-stable sweet protein, mabinlin II." Nirasawa S., Masuda Y., Nakaya K., Kurihara Y. Gene 181:225-227(1996) [PubMed: 8973336] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Seed.
[2]	"Purification, complete amino acid sequence and structural characterization of the heat-stable sweet protein, mabinlin II." Liu X., Maeda S., Hu Z., Aiuchi T., Nakaya K., Kurihara Y. Eur. J. Biochem. 211:281-287(1993) [PubMed: 8425538] [Abstract] Cited for: PROTEIN SEQUENCE OF 36-68 AND 83-154. Tissue: Seed.
[3]	"Disulfide bridge structure of the heat-stable sweet protein mabinlin II." Nirasawa S., Liu X., Nishino T., Kurihara Y. Biochim. Biophys. Acta 1202:277-280(1993) [PubMed: 8399391] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, DISULFIDE BONDS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

D83997 mRNA. Translation: BAA12204.1.

PIR

JC5379.
S28842.
S28843.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2DS2	X-ray	1.70	A/C	36-68	[»]
			B/D	83-154	[»]

ModBase

Family and domain databases

InterPro

IPR016140. Bifunc_inhib/LTP/seed_store.
IPR003612. LTP/seed_store/tryp_amyl_inhib.
IPR000617. Napin.
IPR013771. Trypsin/amylase_inhib.
[Graphical view]

Gene3D

G3DSA:1.10.120.10. Trypsin/amylase_inhib. 1 hit.

Pfam

PF00234. Tryp_alpha_amyl. 1 hit.
[Graphical view]

PRINTS

PR00496. NAPIN.

SMART

SM00499. AAI. 1 hit.
[Graphical view]

ProtoNet

Entry information

Entry name

2SS2_CAPMA

Accession

Primary (citable) accession number: P30233
Secondary accession number(s): O04774

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	February 21, 2001
Last modified:	November 24, 2009
This is version 66 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents