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P30233

- 2SS2_CAPMA

UniProt

P30233 - 2SS2_CAPMA

Protein

Sweet protein mabinlin-2

Gene
N/A
Organism
Capparis masaikai (Mabinlang)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Heat stable 2S seed storage protein having sweetness-inducing activity.

    GO - Molecular functioni

    1. nutrient reservoir activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Seed storage protein, Storage protein, Taste-modifying protein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sweet protein mabinlin-2
    Alternative name(s):
    Mabinlin II
    Short name:
    MAB II
    Cleaved into the following 2 chains:
    OrganismiCapparis masaikai (Mabinlang)
    Taxonomic identifieri13395 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesCapparaceaeCapparis

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Propeptidei21 – 35151 PublicationPRO_0000032137Add
    BLAST
    Chaini36 – 6833Sweet protein mabinlin-2 chain APRO_0000032138Add
    BLAST
    Propeptidei69 – 82141 PublicationPRO_0000032139Add
    BLAST
    Chaini83 – 15472Sweet protein mabinlin-2 chain BPRO_0000032140Add
    BLAST
    Propeptidei155 – 1551PRO_0000032141

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei36 – 361Pyrrolidone carboxylic acid
    Disulfide bondi40 ↔ 103Interchain (between A and B chains)
    Disulfide bondi53 ↔ 92Interchain (between A and B chains)
    Modified residuei83 – 831Pyrrolidone carboxylic acid
    Disulfide bondi93 ↔ 141
    Disulfide bondi105 ↔ 149

    Keywords - PTMi

    Disulfide bond, Pyrrolidone carboxylic acid

    Interactioni

    Subunit structurei

    Heterodimer of a small A and a large B chain linked by disulfide bonds.

    Structurei

    Secondary structure

    1
    155
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi40 – 467
    Helixi47 – 504
    Helixi51 – 6212
    Helixi88 – 969
    Helixi101 – 1033
    Helixi104 – 11815
    Helixi124 – 14017

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2DS2X-ray1.70A/C36-68[»]
    B/D83-154[»]
    ProteinModelPortaliP30233.
    SMRiP30233. Positions 37-64, 86-153.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP30233.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the 2S seed storage albumins family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    InterProiIPR016140. Bifunc_inhib/LTP/seed_store.
    IPR000617. Napin.
    [Graphical view]
    PfamiPF00234. Tryp_alpha_amyl. 1 hit.
    [Graphical view]
    PRINTSiPR00496. NAPIN.
    SMARTiSM00499. AAI. 1 hit.
    [Graphical view]
    SUPFAMiSSF47699. SSF47699. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P30233-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKLIFLFAT LALFVLLANA SIQTTVIEVD EEEDNQLWRC QRQFLQHQRL    50
    RACQRFIHRR AQFGGQPDEL EDEVEDDNDD ENQPRRPALR QCCNQLRQVD 100
    RPCVCPVLRQ AAQQVLQRQI IQGPQQLRRL FDAARNLPNI CNIPNIGACP 150
    FRAWP 155
    Length:155
    Mass (Da):18,089
    Last modified:February 21, 2001 - v3
    Checksum:i72E885DEDCC2D46A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti148 – 1481A → T in BAA12204. (PubMed:8973336)Curated
    Sequence conflicti153 – 1531A → T in BAA12204. (PubMed:8973336)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D83997 mRNA. Translation: BAA12204.1.
    PIRiJC5379.
    S28842.
    S28843.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D83997 mRNA. Translation: BAA12204.1 .
    PIRi JC5379.
    S28842.
    S28843.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2DS2 X-ray 1.70 A/C 36-68 [» ]
    B/D 83-154 [» ]
    ProteinModelPortali P30233.
    SMRi P30233. Positions 37-64, 86-153.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P30233.

    Family and domain databases

    InterProi IPR016140. Bifunc_inhib/LTP/seed_store.
    IPR000617. Napin.
    [Graphical view ]
    Pfami PF00234. Tryp_alpha_amyl. 1 hit.
    [Graphical view ]
    PRINTSi PR00496. NAPIN.
    SMARTi SM00499. AAI. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47699. SSF47699. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of a cDNA encoding a heat-stable sweet protein, mabinlin II."
      Nirasawa S., Masuda Y., Nakaya K., Kurihara Y.
      Gene 181:225-227(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Seed.
    2. "Purification, complete amino acid sequence and structural characterization of the heat-stable sweet protein, mabinlin II."
      Liu X., Maeda S., Hu Z., Aiuchi T., Nakaya K., Kurihara Y.
      Eur. J. Biochem. 211:281-287(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 36-68 AND 83-154.
      Tissue: Seed.
    3. "Disulfide bridge structure of the heat-stable sweet protein mabinlin II."
      Nirasawa S., Liu X., Nishino T., Kurihara Y.
      Biochim. Biophys. Acta 1202:277-280(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE, DISULFIDE BONDS.
    4. "Crystal structure of Mabinlin II: a novel structural type of sweet proteins and the main structural basis for its sweetness."
      Li D.F., Jiang P., Zhu D.Y., Hu Y., Max M., Wang D.C.
      J. Struct. Biol. 162:50-62(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 36-68 AND 83-154, DISULFIDE BONDS.

    Entry informationi

    Entry namei2SS2_CAPMA
    AccessioniPrimary (citable) accession number: P30233
    Secondary accession number(s): O04774
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: February 21, 2001
    Last modified: October 1, 2014
    This is version 82 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3