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P30233

- 2SS2_CAPMA

UniProt

P30233 - 2SS2_CAPMA

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Protein
Sweet protein mabinlin-2
Gene
N/A
Organism
Capparis masaikai (Mabinlang)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Heat stable 2S seed storage protein having sweetness-inducing activity.

GO - Molecular functioni

  1. nutrient reservoir activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Seed storage protein, Storage protein, Taste-modifying protein

Names & Taxonomyi

Protein namesi
Recommended name:
Sweet protein mabinlin-2
Alternative name(s):
Mabinlin II
Short name:
MAB II
Cleaved into the following 2 chains:
OrganismiCapparis masaikai (Mabinlang)
Taxonomic identifieri13395 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesCapparaceaeCapparis

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020 Reviewed prediction
Add
BLAST
Propeptidei21 – 3515
PRO_0000032137Add
BLAST
Chaini36 – 6833Sweet protein mabinlin-2 chain A
PRO_0000032138Add
BLAST
Propeptidei69 – 8214
PRO_0000032139Add
BLAST
Chaini83 – 15472Sweet protein mabinlin-2 chain B
PRO_0000032140Add
BLAST
Propeptidei155 – 1551
PRO_0000032141

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei36 – 361Pyrrolidone carboxylic acid
Disulfide bondi40 ↔ 103Interchain (between A and B chains)2 Publications
Disulfide bondi53 ↔ 92Interchain (between A and B chains)2 Publications
Modified residuei83 – 831Pyrrolidone carboxylic acid
Disulfide bondi93 ↔ 1412 Publications
Disulfide bondi105 ↔ 1492 Publications

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Interactioni

Subunit structurei

Heterodimer of a small A and a large B chain linked by disulfide bonds.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi40 – 467
Helixi47 – 504
Helixi51 – 6212
Helixi88 – 969
Helixi101 – 1033
Helixi104 – 11815
Helixi124 – 14017

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DS2X-ray1.70A/C36-68[»]
B/D83-154[»]
ProteinModelPortaliP30233.
SMRiP30233. Positions 37-64, 86-153.

Miscellaneous databases

EvolutionaryTraceiP30233.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR016140. Bifunc_inhib/LTP/seed_store.
IPR000617. Napin.
[Graphical view]
PfamiPF00234. Tryp_alpha_amyl. 1 hit.
[Graphical view]
PRINTSiPR00496. NAPIN.
SMARTiSM00499. AAI. 1 hit.
[Graphical view]
SUPFAMiSSF47699. SSF47699. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30233-1 [UniParc]FASTAAdd to Basket

« Hide

MAKLIFLFAT LALFVLLANA SIQTTVIEVD EEEDNQLWRC QRQFLQHQRL    50
RACQRFIHRR AQFGGQPDEL EDEVEDDNDD ENQPRRPALR QCCNQLRQVD 100
RPCVCPVLRQ AAQQVLQRQI IQGPQQLRRL FDAARNLPNI CNIPNIGACP 150
FRAWP 155
Length:155
Mass (Da):18,089
Last modified:February 21, 2001 - v3
Checksum:i72E885DEDCC2D46A
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti148 – 1481A → T in BAA12204. 1 Publication
Sequence conflicti153 – 1531A → T in BAA12204. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D83997 mRNA. Translation: BAA12204.1.
PIRiJC5379.
S28842.
S28843.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D83997 mRNA. Translation: BAA12204.1 .
PIRi JC5379.
S28842.
S28843.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2DS2 X-ray 1.70 A/C 36-68 [» ]
B/D 83-154 [» ]
ProteinModelPortali P30233.
SMRi P30233. Positions 37-64, 86-153.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P30233.

Family and domain databases

InterProi IPR016140. Bifunc_inhib/LTP/seed_store.
IPR000617. Napin.
[Graphical view ]
Pfami PF00234. Tryp_alpha_amyl. 1 hit.
[Graphical view ]
PRINTSi PR00496. NAPIN.
SMARTi SM00499. AAI. 1 hit.
[Graphical view ]
SUPFAMi SSF47699. SSF47699. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequencing of a cDNA encoding a heat-stable sweet protein, mabinlin II."
    Nirasawa S., Masuda Y., Nakaya K., Kurihara Y.
    Gene 181:225-227(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Seed.
  2. "Purification, complete amino acid sequence and structural characterization of the heat-stable sweet protein, mabinlin II."
    Liu X., Maeda S., Hu Z., Aiuchi T., Nakaya K., Kurihara Y.
    Eur. J. Biochem. 211:281-287(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 36-68 AND 83-154.
    Tissue: Seed.
  3. "Disulfide bridge structure of the heat-stable sweet protein mabinlin II."
    Nirasawa S., Liu X., Nishino T., Kurihara Y.
    Biochim. Biophys. Acta 1202:277-280(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE, DISULFIDE BONDS.
  4. "Crystal structure of Mabinlin II: a novel structural type of sweet proteins and the main structural basis for its sweetness."
    Li D.F., Jiang P., Zhu D.Y., Hu Y., Max M., Wang D.C.
    J. Struct. Biol. 162:50-62(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 36-68 AND 83-154, DISULFIDE BONDS.

Entry informationi

Entry namei2SS2_CAPMA
AccessioniPrimary (citable) accession number: P30233
Secondary accession number(s): O04774
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: February 21, 2001
Last modified: February 19, 2014
This is version 81 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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