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P30233 (2SS2_CAPMA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sweet protein mabinlin-2
Alternative name(s):
Mabinlin II
Short name=MAB II
OrganismCapparis masaikai (Mabinlang)
Taxonomic identifier13395 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesCapparaceaeCapparis

Protein attributes

Sequence length155 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Heat stable 2S seed storage protein having sweetness-inducing activity.

Subunit structure

Heterodimer of a small A and a large B chain linked by disulfide bonds.

Sequence similarities

Belongs to the 2S seed storage albumins family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Propeptide21 – 3515
PRO_0000032137
Chain36 – 6833Sweet protein mabinlin-2 chain A
PRO_0000032138
Propeptide69 – 8214
PRO_0000032139
Chain83 – 15472Sweet protein mabinlin-2 chain B
PRO_0000032140
Propeptide1551
PRO_0000032141

Amino acid modifications

Modified residue361Pyrrolidone carboxylic acid
Modified residue831Pyrrolidone carboxylic acid
Disulfide bond40 ↔ 103Interchain (between A and B chains) Ref.3 Ref.4
Disulfide bond53 ↔ 92Interchain (between A and B chains) Ref.3 Ref.4
Disulfide bond93 ↔ 141 Ref.3 Ref.4
Disulfide bond105 ↔ 149 Ref.3 Ref.4

Experimental info

Sequence conflict1481A → T in BAA12204. Ref.1
Sequence conflict1531A → T in BAA12204. Ref.1

Secondary structure

............ 155
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P30233 [UniParc].

Last modified February 21, 2001. Version 3.
Checksum: 72E885DEDCC2D46A

FASTA15518,089
        10         20         30         40         50         60 
MAKLIFLFAT LALFVLLANA SIQTTVIEVD EEEDNQLWRC QRQFLQHQRL RACQRFIHRR 

        70         80         90        100        110        120 
AQFGGQPDEL EDEVEDDNDD ENQPRRPALR QCCNQLRQVD RPCVCPVLRQ AAQQVLQRQI 

       130        140        150 
IQGPQQLRRL FDAARNLPNI CNIPNIGACP FRAWP 

« Hide

References

[1]"Cloning and sequencing of a cDNA encoding a heat-stable sweet protein, mabinlin II."
Nirasawa S., Masuda Y., Nakaya K., Kurihara Y.
Gene 181:225-227(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Seed.
[2]"Purification, complete amino acid sequence and structural characterization of the heat-stable sweet protein, mabinlin II."
Liu X., Maeda S., Hu Z., Aiuchi T., Nakaya K., Kurihara Y.
Eur. J. Biochem. 211:281-287(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 36-68 AND 83-154.
Tissue: Seed.
[3]"Disulfide bridge structure of the heat-stable sweet protein mabinlin II."
Nirasawa S., Liu X., Nishino T., Kurihara Y.
Biochim. Biophys. Acta 1202:277-280(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE, DISULFIDE BONDS.
[4]"Crystal structure of Mabinlin II: a novel structural type of sweet proteins and the main structural basis for its sweetness."
Li D.F., Jiang P., Zhu D.Y., Hu Y., Max M., Wang D.C.
J. Struct. Biol. 162:50-62(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 36-68 AND 83-154, DISULFIDE BONDS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D83997 mRNA. Translation: BAA12204.1.
PIRJC5379.
S28842.
S28843.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2DS2X-ray1.70A/C36-68[»]
B/D83-154[»]
ProteinModelPortalP30233.
SMRP30233. Positions 37-64, 86-153.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR016140. Bifunc_inhib/LTP/seed_store.
IPR000617. Napin.
[Graphical view]
PfamPF00234. Tryp_alpha_amyl. 1 hit.
[Graphical view]
PRINTSPR00496. NAPIN.
SMARTSM00499. AAI. 1 hit.
[Graphical view]
SUPFAMSSF47699. SSF47699. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP30233.

Entry information

Entry name2SS2_CAPMA
AccessionPrimary (citable) accession number: P30233
Secondary accession number(s): O04774
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: February 21, 2001
Last modified: February 19, 2014
This is version 81 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references