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P30233

- 2SS2_CAPMA

UniProt

P30233 - 2SS2_CAPMA

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Protein

Sweet protein mabinlin-2

Gene
N/A
Organism
Capparis masaikai (Mabinlang)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Heat stable 2S seed storage protein having sweetness-inducing activity.

GO - Molecular functioni

  1. nutrient reservoir activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Seed storage protein, Storage protein, Taste-modifying protein

Names & Taxonomyi

Protein namesi
Recommended name:
Sweet protein mabinlin-2
Alternative name(s):
Mabinlin II
Short name:
MAB II
Cleaved into the following 2 chains:
OrganismiCapparis masaikai (Mabinlang)
Taxonomic identifieri13395 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesCapparaceaeCapparis

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Propeptidei21 – 35151 PublicationPRO_0000032137Add
BLAST
Chaini36 – 6833Sweet protein mabinlin-2 chain APRO_0000032138Add
BLAST
Propeptidei69 – 82141 PublicationPRO_0000032139Add
BLAST
Chaini83 – 15472Sweet protein mabinlin-2 chain BPRO_0000032140Add
BLAST
Propeptidei155 – 1551PRO_0000032141

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei36 – 361Pyrrolidone carboxylic acid
Disulfide bondi40 ↔ 103Interchain (between A and B chains)
Disulfide bondi53 ↔ 92Interchain (between A and B chains)
Modified residuei83 – 831Pyrrolidone carboxylic acid
Disulfide bondi93 ↔ 141
Disulfide bondi105 ↔ 149

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Interactioni

Subunit structurei

Heterodimer of a small A and a large B chain linked by disulfide bonds.

Structurei

Secondary structure

1
155
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi40 – 467
Helixi47 – 504
Helixi51 – 6212
Helixi88 – 969
Helixi101 – 1033
Helixi104 – 11815
Helixi124 – 14017

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DS2X-ray1.70A/C36-68[»]
B/D83-154[»]
ProteinModelPortaliP30233.
SMRiP30233. Positions 37-64, 86-153.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP30233.

Family & Domainsi

Sequence similaritiesi

Belongs to the 2S seed storage albumins family.Curated

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR016140. Bifunc_inhib/LTP/seed_store.
IPR000617. Napin.
[Graphical view]
PfamiPF00234. Tryp_alpha_amyl. 1 hit.
[Graphical view]
PRINTSiPR00496. NAPIN.
SMARTiSM00499. AAI. 1 hit.
[Graphical view]
SUPFAMiSSF47699. SSF47699. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30233-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAKLIFLFAT LALFVLLANA SIQTTVIEVD EEEDNQLWRC QRQFLQHQRL
60 70 80 90 100
RACQRFIHRR AQFGGQPDEL EDEVEDDNDD ENQPRRPALR QCCNQLRQVD
110 120 130 140 150
RPCVCPVLRQ AAQQVLQRQI IQGPQQLRRL FDAARNLPNI CNIPNIGACP

FRAWP
Length:155
Mass (Da):18,089
Last modified:February 21, 2001 - v3
Checksum:i72E885DEDCC2D46A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti148 – 1481A → T in BAA12204. (PubMed:8973336)Curated
Sequence conflicti153 – 1531A → T in BAA12204. (PubMed:8973336)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D83997 mRNA. Translation: BAA12204.1.
PIRiJC5379.
S28842.
S28843.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D83997 mRNA. Translation: BAA12204.1 .
PIRi JC5379.
S28842.
S28843.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2DS2 X-ray 1.70 A/C 36-68 [» ]
B/D 83-154 [» ]
ProteinModelPortali P30233.
SMRi P30233. Positions 37-64, 86-153.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P30233.

Family and domain databases

InterProi IPR016140. Bifunc_inhib/LTP/seed_store.
IPR000617. Napin.
[Graphical view ]
Pfami PF00234. Tryp_alpha_amyl. 1 hit.
[Graphical view ]
PRINTSi PR00496. NAPIN.
SMARTi SM00499. AAI. 1 hit.
[Graphical view ]
SUPFAMi SSF47699. SSF47699. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequencing of a cDNA encoding a heat-stable sweet protein, mabinlin II."
    Nirasawa S., Masuda Y., Nakaya K., Kurihara Y.
    Gene 181:225-227(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Seed.
  2. "Purification, complete amino acid sequence and structural characterization of the heat-stable sweet protein, mabinlin II."
    Liu X., Maeda S., Hu Z., Aiuchi T., Nakaya K., Kurihara Y.
    Eur. J. Biochem. 211:281-287(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 36-68 AND 83-154.
    Tissue: Seed.
  3. "Disulfide bridge structure of the heat-stable sweet protein mabinlin II."
    Nirasawa S., Liu X., Nishino T., Kurihara Y.
    Biochim. Biophys. Acta 1202:277-280(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE, DISULFIDE BONDS.
  4. "Crystal structure of Mabinlin II: a novel structural type of sweet proteins and the main structural basis for its sweetness."
    Li D.F., Jiang P., Zhu D.Y., Hu Y., Max M., Wang D.C.
    J. Struct. Biol. 162:50-62(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 36-68 AND 83-154, DISULFIDE BONDS.

Entry informationi

Entry namei2SS2_CAPMA
AccessioniPrimary (citable) accession number: P30233
Secondary accession number(s): O04774
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: February 21, 2001
Last modified: October 1, 2014
This is version 82 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3