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Protein

Hemagglutinin-esterase

Gene

HE

Organism
Human coronavirus OC43 (HCoV-OC43)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-9-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system (By similarity).By similarity

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei40NucleophileBy similarity1
Active sitei326Charge relay systemBy similarity1
Active sitei329Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

  • fusion of virus membrane with host plasma membrane Source: InterPro
  • viral life cycle Source: CACAO
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esterase (EC:3.1.1.53)
Short name:
HE protein
Alternative name(s):
E3 glycoprotein
Gene namesi
Name:HE
ORF Names:2b
OrganismiHuman coronavirus OC43 (HCoV-OC43)
Taxonomic identifieri31631 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000007552 Componenti: Genome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini19 – 392Virion surfaceSequence analysisAdd BLAST374
Transmembranei393 – 413HelicalSequence analysisAdd BLAST21
Topological domaini414 – 424IntravirionSequence analysisAdd BLAST11

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18By similarityAdd BLAST18
ChainiPRO_000003714419 – 424Hemagglutinin-esteraseAdd BLAST406

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi44 ↔ 65By similarity
Glycosylationi54N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi89N-linked (GlcNAc...); by hostSequence analysis1
Disulfide bondi113 ↔ 162By similarity
Glycosylationi114N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi153N-linked (GlcNAc...); by hostSequence analysis1
Disulfide bondi197 ↔ 276By similarity
Disulfide bondi205 ↔ 249By similarity
Glycosylationi236N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi301N-linked (GlcNAc...); by hostSequence analysis1
Disulfide bondi307 ↔ 312By similarity
Glycosylationi316N-linked (GlcNAc...); by hostSequence analysis1
Disulfide bondi347 ↔ 371By similarity
Glycosylationi358N-linked (GlcNAc...); by hostSequence analysis1

Post-translational modificationi

N-glycosylated in the RER.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE.

Structurei

3D structure databases

ProteinModelPortaliP30215.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni7 – 127Esterase domain first partBy similarityAdd BLAST121
Regioni128 – 266Receptor bindingBy similarityAdd BLAST139
Regioni267 – 379Esterase domain second partBy similarityAdd BLAST113

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
IPR013830. SGNH_hydro.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.
SSF52266. SSF52266. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30215-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLLPRFILV SCIIGSLGFY NPPTNVVSHV NGDWFLFGDS RSDCNHIVNI
60 70 80 90 100
NPHNYSYMDL NPVLCDSGKI SSKAGNSIFR SFHFTDFYNY TGEGQQIIFY
110 120 130 140 150
EGVNFTPYHA FKCNRSGSND IWMQNKGLFY TQVYKNMAVY RSLTFVNVPY
160 170 180 190 200
VYNGSAQSTA LCKSGSLVLN NPAYIAPQAN SGDYYYKVEA DFYLSGCDEY
210 220 230 240 250
IVPLCIFNGK FLSNTKYYDD SQYYFNKDTG VIYGLNSTET ITTGFDLNCY
260 270 280 290 300
YLVLPSGNYL AISNELLLTV PTKAICLNKR KDFTPVQVVD SRWNNARQSD
310 320 330 340 350
NMTAVACQPP YCYFRNSTTN YVGVYDINHG DAGFTSILSG LLYNSPCFSQ
360 370 380 390 400
QGVFRYDNVS SVWPLYPYGR CPTAADINNP DLPICVYDPL PVILLGILLG
410 420
VAVIIIVVLL LYFMVDNGTR LHDA
Length:424
Mass (Da):47,724
Last modified:April 1, 1993 - v1
Checksum:iEA43A1CCDF8598D7
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti158S → A in strain: Isolate ATCC VR-759 and Isolate clinical OC43-Paris. 1
Natural varianti379N → I in strain: Isolate ATCC VR-759 and Isolate clinical OC43-Paris. 1
Natural varianti403 – 404VI → IV in strain: Isolate ATCC VR-759 and Isolate clinical OC43-Paris. 2
Natural varianti418G → V in strain: Isolate ATCC VR-759 and Isolate clinical OC43-Paris. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M76373 Genomic RNA. Translation: AAA45460.1.
AY391777 Genomic RNA. Translation: AAR01014.1.
AY585228 Genomic RNA. Translation: AAT84353.1.
AY585229 Genomic RNA. Translation: AAT84361.1.
PIRiA39450. HMIHCC.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M76373 Genomic RNA. Translation: AAA45460.1.
AY391777 Genomic RNA. Translation: AAR01014.1.
AY585228 Genomic RNA. Translation: AAT84353.1.
AY585229 Genomic RNA. Translation: AAT84361.1.
PIRiA39450. HMIHCC.

3D structure databases

ProteinModelPortaliP30215.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
IPR013830. SGNH_hydro.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.
SSF52266. SSF52266. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiHEMA_CVHOC
AccessioniPrimary (citable) accession number: P30215
Secondary accession number(s): Q6TNG0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: November 30, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.