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P30215

- HEMA_CVHOC

UniProt

P30215 - HEMA_CVHOC

Protein

Hemagglutinin-esterase

Gene

HE

Organism
Human coronavirus OC43 (HCoV-OC43)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-9-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system By similarity.By similarity

    Catalytic activityi

    N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei40 – 401NucleophileBy similarity
    Active sitei326 – 3261Charge relay systemBy similarity
    Active sitei329 – 3291Charge relay systemBy similarity

    GO - Molecular functioni

    1. sialate O-acetylesterase activity Source: UniProtKB-EC

    GO - Biological processi

    1. fusion of virus membrane with host plasma membrane Source: InterPro
    2. viral life cycle Source: CACAO

    Keywords - Molecular functioni

    Hemagglutinin, Hydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hemagglutinin-esterase (EC:3.1.1.53)
    Short name:
    HE protein
    Alternative name(s):
    E3 glycoprotein
    Gene namesi
    Name:HE
    ORF Names:2b
    OrganismiHuman coronavirus OC43 (HCoV-OC43)
    Taxonomic identifieri31631 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000007552: Genome

    Subcellular locationi

    Virion membrane Curated; Single-pass type I membrane protein Curated. Host cell membrane Curated; Single-pass type I membrane protein Curated
    Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface By similarity.By similarity

    GO - Cellular componenti

    1. host cell plasma membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. viral envelope Source: UniProtKB-KW
    4. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818By similarityAdd
    BLAST
    Chaini19 – 424406Hemagglutinin-esterasePRO_0000037144Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi44 ↔ 65By similarity
    Glycosylationi54 – 541N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi89 – 891N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi113 ↔ 162By similarity
    Glycosylationi114 – 1141N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi153 – 1531N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi197 ↔ 276By similarity
    Disulfide bondi205 ↔ 249By similarity
    Glycosylationi236 – 2361N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi301 – 3011N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi307 ↔ 312By similarity
    Glycosylationi316 – 3161N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi347 ↔ 371By similarity
    Glycosylationi358 – 3581N-linked (GlcNAc...); by hostSequence Analysis

    Post-translational modificationi

    N-glycosylated in the RER.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE.

    Structurei

    3D structure databases

    ProteinModelPortaliP30215.
    SMRiP30215. Positions 19-376.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini19 – 392374Virion surfaceSequence AnalysisAdd
    BLAST
    Topological domaini414 – 42411IntravirionSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei393 – 41321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni7 – 127121Esterase domain first partBy similarityAdd
    BLAST
    Regioni128 – 266139Receptor bindingBy similarityAdd
    BLAST
    Regioni267 – 379113Esterase domain second partBy similarityAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Family and domain databases

    InterProiIPR008980. Capsid_hemagglutn.
    IPR007142. Hemagglutn-estrase_core.
    IPR003860. Hemagglutn-estrase_hemagglutn.
    [Graphical view]
    PfamiPF03996. Hema_esterase. 1 hit.
    PF02710. Hema_HEFG. 1 hit.
    [Graphical view]
    SUPFAMiSSF49818. SSF49818. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P30215-1 [UniParc]FASTAAdd to Basket

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    MFLLPRFILV SCIIGSLGFY NPPTNVVSHV NGDWFLFGDS RSDCNHIVNI    50
    NPHNYSYMDL NPVLCDSGKI SSKAGNSIFR SFHFTDFYNY TGEGQQIIFY 100
    EGVNFTPYHA FKCNRSGSND IWMQNKGLFY TQVYKNMAVY RSLTFVNVPY 150
    VYNGSAQSTA LCKSGSLVLN NPAYIAPQAN SGDYYYKVEA DFYLSGCDEY 200
    IVPLCIFNGK FLSNTKYYDD SQYYFNKDTG VIYGLNSTET ITTGFDLNCY 250
    YLVLPSGNYL AISNELLLTV PTKAICLNKR KDFTPVQVVD SRWNNARQSD 300
    NMTAVACQPP YCYFRNSTTN YVGVYDINHG DAGFTSILSG LLYNSPCFSQ 350
    QGVFRYDNVS SVWPLYPYGR CPTAADINNP DLPICVYDPL PVILLGILLG 400
    VAVIIIVVLL LYFMVDNGTR LHDA 424
    Length:424
    Mass (Da):47,724
    Last modified:April 1, 1993 - v1
    Checksum:iEA43A1CCDF8598D7
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti158 – 1581S → A in strain: Isolate ATCC VR-759 and Isolate clinical OC43-Paris.
    Natural varianti379 – 3791N → I in strain: Isolate ATCC VR-759 and Isolate clinical OC43-Paris.
    Natural varianti403 – 4042VI → IV in strain: Isolate ATCC VR-759 and Isolate clinical OC43-Paris.
    Natural varianti418 – 4181G → V in strain: Isolate ATCC VR-759 and Isolate clinical OC43-Paris.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M76373 Genomic RNA. Translation: AAA45460.1.
    AY391777 Genomic RNA. Translation: AAR01014.1.
    AY585228 Genomic RNA. Translation: AAT84353.1.
    AY585229 Genomic RNA. Translation: AAT84361.1.
    PIRiA39450. HMIHCC.
    RefSeqiNP_937949.1. NC_005147.1.

    Genome annotation databases

    GeneIDi2648209.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M76373 Genomic RNA. Translation: AAA45460.1 .
    AY391777 Genomic RNA. Translation: AAR01014.1 .
    AY585228 Genomic RNA. Translation: AAT84353.1 .
    AY585229 Genomic RNA. Translation: AAT84361.1 .
    PIRi A39450. HMIHCC.
    RefSeqi NP_937949.1. NC_005147.1.

    3D structure databases

    ProteinModelPortali P30215.
    SMRi P30215. Positions 19-376.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 2648209.

    Family and domain databases

    InterProi IPR008980. Capsid_hemagglutn.
    IPR007142. Hemagglutn-estrase_core.
    IPR003860. Hemagglutn-estrase_hemagglutn.
    [Graphical view ]
    Pfami PF03996. Hema_esterase. 1 hit.
    PF02710. Hema_HEFG. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49818. SSF49818. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The hemagglutinin/esterase gene of human coronavirus strain OC43: phylogenetic relationships to bovine and murine coronaviruses and influenza C virus."
      Zhang X.M., Kousoulas K.G., Storz J.
      Virology 186:318-323(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Human respiratory coronavirus OC43: genetic stability and neuroinvasion."
      St Jean J.R., Jacomy H., Desforges M., Vabret A., Freymuth F., Talbot P.J.
      J. Virol. 78:8824-8834(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate ATCC VR-759 and Isolate clinical OC43-Paris.
    3. "Complete genomic sequence of human coronavirus OC43: molecular clock analysis suggests a relatively recent zoonotic coronavirus transmission event."
      Vijgen L., Keyaerts E., Moes E., Thoelen I., Wollants E., Lemey P., Vandamme A.M., Van Ranst M.
      J. Virol. 79:1595-1604(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate ATCC VR-759.

    Entry informationi

    Entry nameiHEMA_CVHOC
    AccessioniPrimary (citable) accession number: P30215
    Secondary accession number(s): Q6TNG0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3