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Protein

Hemagglutinin-esterase

Gene

HE

Organism
Human coronavirus OC43 (HCoV-OC43)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-4-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. May become a target for both the humoral and the cellular branches of the immune system.UniRule annotation

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei40NucleophileUniRule annotation1
Active sitei326Charge relay systemUniRule annotation1
Active sitei329Charge relay systemUniRule annotation1

GO - Molecular functioni

GO - Biological processi

  • fusion of virus membrane with host plasma membrane Source: InterPro
  • viral life cycle Source: CACAO

Keywordsi

Molecular functionHemagglutinin, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esteraseUniRule annotation (EC:3.1.1.53UniRule annotation)
Short name:
HE proteinUniRule annotation
Alternative name(s):
E3 glycoproteinUniRule annotation
Gene namesi
Name:HEUniRule annotation
ORF Names:2b
OrganismiHuman coronavirus OC43 (HCoV-OC43)
Taxonomic identifieri31631 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000100580 Componenti: Genome
  • UP000007552 Componenti: Genome

Subcellular locationi

  • Virion membrane UniRule annotation; Single-pass type I membrane protein UniRule annotation
  • Host cell membrane UniRule annotation; Single-pass type I membrane protein UniRule annotation
  • Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface.UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini17 – 392Virion surfaceUniRule annotationAdd BLAST376
Transmembranei393 – 413HelicalUniRule annotationAdd BLAST21
Topological domaini414 – 424IntravirionUniRule annotationAdd BLAST11

GO - Cellular componenti

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16UniRule annotationAdd BLAST16
ChainiPRO_000003714417 – 424Hemagglutinin-esteraseUniRule annotationAdd BLAST408

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi44 ↔ 65UniRule annotation
Glycosylationi54N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Glycosylationi89N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Disulfide bondi113 ↔ 162UniRule annotation
Glycosylationi114N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Glycosylationi153N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Disulfide bondi197 ↔ 276UniRule annotation
Disulfide bondi205 ↔ 249UniRule annotation
Glycosylationi236N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Glycosylationi301N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Disulfide bondi307 ↔ 312UniRule annotation
Glycosylationi316N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Disulfide bondi347 ↔ 371UniRule annotation
Glycosylationi358N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Glycosylationi417N-linked (GlcNAc...) asparagine; by hostUniRule annotation1

Post-translational modificationi

N-glycosylated in the host RER.UniRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Forms a complex with the M protein in the pre-Golgi. Associates then with S-M complex to form a ternary complex S-M-HE.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP30215
SMRiP30215
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni7 – 127Esterase domain 1UniRule annotationAdd BLAST121
Regioni128 – 266Receptor bindingUniRule annotationAdd BLAST139
Regioni267 – 379Esterase domain 2UniRule annotationAdd BLAST113

Sequence similaritiesi

Belongs to the influenza type C/coronaviruses hemagglutinin-esterase family.UniRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

OrthoDBiVOG0900009Y

Family and domain databases

HAMAPiMF_04207 BETA_CORONA_HE, 1 hit
InterProiView protein in InterPro
IPR008980 Capsid_hemagglutn
IPR007142 Hemagglutn-estrase_core
IPR003860 Hemagglutn-estrase_hemagglutn
PfamiView protein in Pfam
PF03996 Hema_esterase, 1 hit
PF02710 Hema_HEFG, 1 hit
SUPFAMiSSF49818 SSF49818, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30215-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLLPRFILV SCIIGSLGFY NPPTNVVSHV NGDWFLFGDS RSDCNHIVNI
60 70 80 90 100
NPHNYSYMDL NPVLCDSGKI SSKAGNSIFR SFHFTDFYNY TGEGQQIIFY
110 120 130 140 150
EGVNFTPYHA FKCNRSGSND IWMQNKGLFY TQVYKNMAVY RSLTFVNVPY
160 170 180 190 200
VYNGSAQSTA LCKSGSLVLN NPAYIAPQAN SGDYYYKVEA DFYLSGCDEY
210 220 230 240 250
IVPLCIFNGK FLSNTKYYDD SQYYFNKDTG VIYGLNSTET ITTGFDLNCY
260 270 280 290 300
YLVLPSGNYL AISNELLLTV PTKAICLNKR KDFTPVQVVD SRWNNARQSD
310 320 330 340 350
NMTAVACQPP YCYFRNSTTN YVGVYDINHG DAGFTSILSG LLYNSPCFSQ
360 370 380 390 400
QGVFRYDNVS SVWPLYPYGR CPTAADINNP DLPICVYDPL PVILLGILLG
410 420
VAVIIIVVLL LYFMVDNGTR LHDA
Length:424
Mass (Da):47,724
Last modified:April 1, 1993 - v1
Checksum:iEA43A1CCDF8598D7
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti158S → A in strain: Isolate ATCC VR-759 and Isolate clinical OC43-Paris. 1
Natural varianti379N → I in strain: Isolate ATCC VR-759 and Isolate clinical OC43-Paris. 1
Natural varianti403 – 404VI → IV in strain: Isolate ATCC VR-759 and Isolate clinical OC43-Paris. 2
Natural varianti418G → V in strain: Isolate ATCC VR-759 and Isolate clinical OC43-Paris. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M76373 Genomic RNA Translation: AAA45460.1
AY391777 Genomic RNA Translation: AAR01014.1
AY585228 Genomic RNA Translation: AAT84353.1
AY585229 Genomic RNA Translation: AAT84361.1
PIRiA39450 HMIHCC

Similar proteinsi

Entry informationi

Entry nameiHEMA_CVHOC
AccessioniPrimary (citable) accession number: P30215
Secondary accession number(s): Q6TNG0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: May 23, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health