Non-structural protein 1

Functionⁱ

Seems to induce the proteasome-dependent degradation of IRF3, IRF5 and IRF7, thereby antagonizing the cellular interferon response and establishment of the antiviral state. Binds and targets IRF3 early post-infection and suppresses IRF3 nuclear translocation (By similarity).By similarity

Regions

Feature key	Position(s)	Length	Description	Graphical view	Feature identifier	Actions
Zinc fingerⁱ	42 – 79	38	Sequence Analysis			Add BLAST

GO - Molecular functionⁱ

metal ion binding Source: UniProtKB-KW
RNA binding Source: UniProtKB-KW

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Non-structural protein 1 Short name: NSP1 Alternative name(s): NCVP2 Non-structural RNA-binding protein 53 Short name: NS53
Organismⁱ	Rotavirus A (isolate Human/Japan/IGV-80-3/198? Gx-Px[x]-Ix-Rx-Cx-Mx-Ax-Nx-Tx-Ex-Hx) (RV-A)
Taxonomic identifierⁱ	31574 [NCBI]
Taxonomic lineageⁱ	Viruses › dsRNA viruses › Reoviridae › Sedoreovirinae › Rotavirus › Rotavirus A › Rotavirus A isolates
Virus hostⁱ	Homo sapiens (Human) [TaxID: 9606]

Subcellular locationⁱ

Host cytoplasm › host cytoskeleton By similarity

GO - Cellular componentⁱ

host cell cytoplasm Source: UniProtKB-KW
host cytoskeleton Source: UniProtKB-SubCell

Complete GO annotation...

Keywords - Cellular componentⁱ

Host cytoplasm, Host cytoskeleton

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	Length	Description	Graphical view	Feature identifier	Actions
Chainⁱ	1 – 486	486	Non-structural protein 1		PRO_0000149556	Add BLAST

Interactionⁱ

Subunit structureⁱ

Interacts (via C-terminus) with host IRF3; this interaction leads to IRF3 degradation. Interacts with host IRF7; this interaction leads to IRF7 degradation (By similarity).By similarity

Family & Domainsⁱ

Region

Feature key	Position(s)	Length	Description	Actions
Regionⁱ	1 – 81	81	RNA-bindingBy similarity	Add BLAST
Regionⁱ	82 – 176	95	Important for cytoskeleton localizationBy similarity	Add BLAST
Regionⁱ	313 – 486	174	Interaction with IRF3By similarity	Add BLAST

Domainⁱ

The zinc-finger domain is important, but not sufficient for binding and degrading IRF3. It is sometimes described as a RING zinc-finger, but it is atypical and it is unclear whether it is related with ubiquitin ligase activity (By similarity).By similarity

Sequence similaritiesⁱ

Belongs to the rotavirus A NSP1 family.Curated

Zinc finger

Feature key	Position(s)	Length	Description	Graphical view	Feature identifier	Actions
Zinc fingerⁱ	42 – 79	38	Sequence Analysis			Add BLAST

Keywords - Domainⁱ

Zinc-finger

Family and domain databases

InterProⁱ	IPR002148. Rotavirus_NSP1. [Graphical view]
Pfamⁱ	PF00981. Rota_NS53. 1 hit. [Graphical view]

Sequenceⁱ

Sequence statusⁱ: Complete.

P30212-1 [UniParc]FASTA Add to Basket

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        10         20         30         40         50
MATFKDACYH YKRINKLNHT VLKLGVNDTW RPSPPTKYKG WCLDCCQHTD 
        60         70         80         90        100
LTYCRGCTMY HVCQWCSQYG RCFLDNEPHL LRMRTFKNEV TKDDLKNLID 
       110        120        130        140        150
MYETLFPMNQ RIVCRFINNT RQHKCRNECM TQWYNHLLLP ITLQSMSIEL 
       160        170        180        190        200
DGDVYYVFGY YDNMNSINQT PFSFTNLVDI YDKLLLDDVN FTRMSFLPAS 
       210        220        230        240        250
LQQEYALRYF SKSRFISEQR KCVNDSHFSI NVIENLHNPS FKVQITRNCS 
       260        270        280        290        300
ELSFDWNKAC KLVKKISAYF DILKTSHIEF YSVSTRCRIF TQCKLKMASK 
       310        320        330        340        350
LIKPNYITSN HKTLATEVHN CKWCSVNNSY TVWNDFRIKN IYDNIFNFLR 
       360        370        380        390        400
ALVKSNVNIG HCSSQEKIYE YVEDVLNVCD DERWKTSIME IFNCLEPVEL 
       410        420        430        440        450
DDVKYVLLNH EINWDVINVL VHSIGKVPQI LTLENVIAIM QSIIYEWFDI 
       460        470        480 
RYMRNTPMVT FTIDKLRRLH TGLKTVDYDS GISDIE

Length:486

Mass (Da):57,645

Last modified:April 1, 1993 - v1

Checksum:ⁱ6A9E6033A3488D2B

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X59297 Genomic RNA. Translation: CAA41986.1.
PIRⁱ	S15470.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X59297 Genomic RNA. Translation: CAA41986.1 .
PIRⁱ	S15470.

3D structure databases

ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Family and domain databases

InterProⁱ	IPR002148. Rotavirus_NSP1. [Graphical view ]
Pfamⁱ	PF00981. Rota_NS53. 1 hit. [Graphical view ]
ProtoNetⁱ	Search...

Publicationsⁱ

Kawagishi M., Matsuno S.
Submitted (MAY-1991) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationⁱ

Entry nameⁱ

NSP1_ROTHI

Accessionⁱ

Primary (citable) accession number: P30212

Entry historyⁱ

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	January 7, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry statusⁱ

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program

Miscellaneousⁱ

Documents

SIMILARITY comments
Index of protein domains and families

External Data

Dasty 3

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

UniProtKB

Proteomes

UniRef

Supporting data

UniParc

Help

P30212

- NSP1_ROTHI

UniProt

P30212 - NSP1_ROTHI

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<p>Provides any useful information about the protein, mostly biological knowledge.</p><p><a href='../manual/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:</p><p><a href='../manual/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:</p><p><a href='../manual/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>Provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.</p><p><a href='../manual/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>Provides information on the location and the topology of the mature protein in the cell.</p><p><a href='../manual/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:</p><p><a href='../manual/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>Describes post-translational modifications (PTMs) and/or processing events.</p><p><a href='../manual/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>Provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.</p><p><a href='../manual/interaction_section' target='_top'>More...</a></p>Interactioni

<p>Provides information on sequence similarities with other proteins and the domain(s) present in a protein.</p><p><a href='../manual/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>Provides information about the sequence similarity with other proteins.</p><p><a href='../manual/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Zinc finger

Family and domain databases

<p>Displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.</p><p><a href='../manual/sequences_section' target='_top'>More...</a></p>Sequencei

Sequence databases

<p>Is used to point to information related to entries and found in data collections other than UniProtKB.</p><p><a href='../manual/cross_references_section' target='_top'>More...</a></p> Cross-referencesi

Sequence databases

3D structure databases

Protocols and materials databases

Family and domain databases

<p>Contains the literature citations that are the sources of data used to annotate the entry. Each reference is numbered and contains several subsections allowing a precise description of a given citation.</p><p><a href='../manual/publications_section' target='_top'>More...</a></p>Publicationsi

<p>Provides general information on the entry.</p><p><a href='../manual/entry_information_section' target='_top'>More...</a></p>Entry informationi

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Documents

External Data

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Publicationsⁱ

Entry informationⁱ

Miscellaneousⁱ