UniProtKB - P30212 (NSP1_ROTHI)
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Protein
Non-structural protein 1
Gene
N/A
Organism
Rotavirus A (isolate RVA/Human/Japan/IGV-80-3/XXXX/GXP[X]) (RV-A)
Status
Functioni
Plays a role in the inhibition of host innate immunity by inducing the degradation of key host factors required to activate interferon production such as IRF3, IRF5 or IRF7. Associates with components of cullin RING ligases (CRLs) including CUL1 or CUL3, which are essential multisubunit ubiquitination complexes, to modulate their activities. Recognizes the host NF-kappa-B regulator BTRC through the presence of a DSGXS motif in the C-terminal substrate recognition domain.UniRule annotation
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- RNA binding Source: UniProtKB-KW
GO - Biological processi
- suppression by virus of host IRF3 activity Source: UniProtKB-KW
- suppression by virus of host IRF7 activity Source: UniProtKB-KW
- suppression by virus of host NF-kappaB transcription factor activity Source: UniProtKB-KW
Keywordsi
Names & Taxonomyi
Protein namesi | Recommended name: Non-structural protein 1UniRule annotationShort name: NSP1UniRule annotation Alternative name(s): NCVP2UniRule annotation Non-structural RNA-binding protein 53UniRule annotation Short name: NS53UniRule annotation |
Organismi | Rotavirus A (isolate RVA/Human/Japan/IGV-80-3/XXXX/GXP[X]) (RV-A) |
Taxonomic identifieri | 31574 [NCBI] |
Taxonomic lineagei | Viruses › dsRNA viruses › Reoviridae › Sedoreovirinae › Rotavirus › Rotavirus A › |
Virus hosti | Homo sapiens (Human) [TaxID: 9606] |
Subcellular locationi
- host cytoskeleton UniRule annotation
GO - Cellular componenti
- host cell cytoplasm Source: UniProtKB-KW
- host cytoskeleton Source: UniProtKB-SubCell
Keywords - Cellular componenti
Host cytoplasm, Host cytoskeletonPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000149556 | 1 – 486 | Non-structural protein 1Add BLAST | 486 |
Post-translational modificationi
The C-terminal region is phosphorylated by host CKII/CSNK2A1. Phosphorylation of the DSGXS motif is essential for host NF-kappa-B inhibition.UniRule annotation
Keywords - PTMi
PhosphoproteinInteractioni
Subunit structurei
Interacts (via C-terminus) with host IRF3; this interaction leads to IRF3 degradation. Interacts with host IRF7; this interaction leads to IRF7 degradation. Interacts with host CUL1 and CUL3. Interacts with host BTRC.UniRule annotation
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 81 | RNA-bindingUniRule annotationAdd BLAST | 81 | |
Regioni | 42 – 79 | Zinc-binding domainUniRule annotationAdd BLAST | 38 | |
Regioni | 82 – 176 | Important for cytoskeleton localizationUniRule annotationAdd BLAST | 95 | |
Regioni | 317 – 486 | Interaction with host IRF3UniRule annotationAdd BLAST | 170 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 479 – 483 | IKBKB-like degron (ILD) motifUniRule annotation | 5 |
Domaini
The integrity of the zinc-binding domain in NSP1 is important for degradation of host IRF3.UniRule annotation
Sequence similaritiesi
Belongs to the rotavirus NSP1 family.UniRule annotation
Family and domain databases
HAMAPi | MF_04088. ROTA_NSP1. 1 hit. |
InterProi | View protein in InterPro IPR002148. Rotavirus_NSP1. |
Pfami | View protein in Pfam PF00981. Rota_NS53. 1 hit. |
i Sequence
Sequence statusi: Complete.
P30212-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATFKDACYH YKRINKLNHT VLKLGVNDTW RPSPPTKYKG WCLDCCQHTD
60 70 80 90 100
LTYCRGCTMY HVCQWCSQYG RCFLDNEPHL LRMRTFKNEV TKDDLKNLID
110 120 130 140 150
MYETLFPMNQ RIVCRFINNT RQHKCRNECM TQWYNHLLLP ITLQSMSIEL
160 170 180 190 200
DGDVYYVFGY YDNMNSINQT PFSFTNLVDI YDKLLLDDVN FTRMSFLPAS
210 220 230 240 250
LQQEYALRYF SKSRFISEQR KCVNDSHFSI NVIENLHNPS FKVQITRNCS
260 270 280 290 300
ELSFDWNKAC KLVKKISAYF DILKTSHIEF YSVSTRCRIF TQCKLKMASK
310 320 330 340 350
LIKPNYITSN HKTLATEVHN CKWCSVNNSY TVWNDFRIKN IYDNIFNFLR
360 370 380 390 400
ALVKSNVNIG HCSSQEKIYE YVEDVLNVCD DERWKTSIME IFNCLEPVEL
410 420 430 440 450
DDVKYVLLNH EINWDVINVL VHSIGKVPQI LTLENVIAIM QSIIYEWFDI
460 470 480
RYMRNTPMVT FTIDKLRRLH TGLKTVDYDS GISDIE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X59297 Genomic RNA. Translation: CAA41986.1. |
PIRi | S15470. |
Similar proteinsi
Entry informationi
Entry namei | NSP1_ROTHI | |
Accessioni | P30212Primary (citable) accession number: P30212 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1993 |
Last sequence update: | April 1, 1993 | |
Last modified: | March 28, 2018 | |
This is version 57 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |