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P30212 - NSP1_ROTHI

UniProt

P30212 - NSP1_ROTHI

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Protein

Non-structural protein 1

Gene
N/A
Organism
Rotavirus A (isolate Human/Japan/IGV-80-3/198? Gx-Px[x]-Ix-Rx-Cx-Mx-Ax-Nx-Tx-Ex-Hx) (RV-A)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Seems to induce the proteasome-dependent degradation of IRF3, IRF5 and IRF7, thereby antagonizing the cellular interferon response and establishment of the antiviral state. Binds and targets IRF3 early post-infection and suppresses IRF3 nuclear translocation (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri42 – 7938Sequence AnalysisAdd
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. suppression by virus of host IRF3 activity Source: UniProtKB-KW
  2. suppression by virus of host IRF7 activity Source: UniProtKB-KW
  3. suppression by virus of host NF-kappaB transcription factor activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host IRF3 by virus, Inhibition of host IRF7 by virus, Inhibition of host NF-kappa-B by virus, Inhibition of host RLR pathway by virus, Interferon antiviral system evasion, Viral immunoevasion

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 1
Short name:
NSP1
Alternative name(s):
NCVP2
Non-structural RNA-binding protein 53
Short name:
NS53
OrganismiRotavirus A (isolate Human/Japan/IGV-80-3/198? Gx-Px[x]-Ix-Rx-Cx-Mx-Ax-Nx-Tx-Ex-Hx) (RV-A)
Taxonomic identifieri31574 [NCBI]
Taxonomic lineageiViruses › dsRNA viruses › Reoviridae › Sedoreovirinae › Rotavirus › Rotavirus A ›
Virus hostiHomo sapiens (Human) [TaxID: 9606]

Subcellular locationi

Host cytoplasm › host cytoskeleton By similarity

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. host cytoskeleton Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 486486Non-structural protein 1PRO_0000149556Add
BLAST

Interactioni

Subunit structurei

Interacts (via C-terminus) with host IRF3; this interaction leads to IRF3 degradation. Interacts with host IRF7; this interaction leads to IRF7 degradation (By similarity).By similarity

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 8181RNA-bindingBy similarityAdd
BLAST
Regioni82 – 17695Important for cytoskeleton localizationBy similarityAdd
BLAST
Regioni313 – 486174Interaction with IRF3By similarityAdd
BLAST

Domaini

The zinc-finger domain is important, but not sufficient for binding and degrading IRF3. It is sometimes described as a RING zinc-finger, but it is atypical and it is unclear whether it is related with ubiquitin ligase activity (By similarity).By similarity

Sequence similaritiesi

Belongs to the rotavirus A NSP1 family.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri42 – 7938Sequence AnalysisAdd
BLAST

Keywords - Domaini

Zinc-finger

Family and domain databases

InterProiIPR002148. Rotavirus_NSP1.
[Graphical view]
PfamiPF00981. Rota_NS53. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30212-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATFKDACYH YKRINKLNHT VLKLGVNDTW RPSPPTKYKG WCLDCCQHTD 
        60         70         80         90        100
LTYCRGCTMY HVCQWCSQYG RCFLDNEPHL LRMRTFKNEV TKDDLKNLID 
       110        120        130        140        150
MYETLFPMNQ RIVCRFINNT RQHKCRNECM TQWYNHLLLP ITLQSMSIEL 
       160        170        180        190        200
DGDVYYVFGY YDNMNSINQT PFSFTNLVDI YDKLLLDDVN FTRMSFLPAS 
       210        220        230        240        250
LQQEYALRYF SKSRFISEQR KCVNDSHFSI NVIENLHNPS FKVQITRNCS 
       260        270        280        290        300
ELSFDWNKAC KLVKKISAYF DILKTSHIEF YSVSTRCRIF TQCKLKMASK 
       310        320        330        340        350
LIKPNYITSN HKTLATEVHN CKWCSVNNSY TVWNDFRIKN IYDNIFNFLR 
       360        370        380        390        400
ALVKSNVNIG HCSSQEKIYE YVEDVLNVCD DERWKTSIME IFNCLEPVEL 
       410        420        430        440        450
DDVKYVLLNH EINWDVINVL VHSIGKVPQI LTLENVIAIM QSIIYEWFDI 
       460        470        480 
RYMRNTPMVT FTIDKLRRLH TGLKTVDYDS GISDIE
Length:486
Mass (Da):57,645
Last modified:April 1, 1993 - v1
Checksum:i6A9E6033A3488D2B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59297 Genomic RNA. Translation: CAA41986.1.
PIRiS15470.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59297 Genomic RNA. Translation: CAA41986.1.
PIRiS15470.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR002148. Rotavirus_NSP1.
[Graphical view]
PfamiPF00981. Rota_NS53. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Kawagishi M., Matsuno S.
    Submitted (MAY-1991) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiNSP1_ROTHI
AccessioniPrimary (citable) accession number: P30212
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: January 7, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.