P30212 (NSP1_ROTHI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Non-structural protein 1 Short name=NSP1 Alternative name(s): NCVP2 Non-structural RNA-binding protein 53 Short name=NS53
Organism	Rotavirus A (isolate Human/Japan/IGV-80-3/198? Gx-Px[x]-Ix-Rx-Cx-Mx-Ax-Nx-Tx-Ex-Hx) (RV-A)
Taxonomic identifier	31574 [NCBI]
Taxonomic lineage	Viruses › dsRNA viruses › Reoviridae › Sedoreovirinae › Rotavirus › Rotavirus A › Rotavirus A isolates
Virus host	Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Seems to induce the proteasome-dependent degradation of IRF3, IRF5 and IRF7, thereby antagonizing the cellular interferon response and establishment of the antiviral state. Binds and targets IRF3 early post-infection and suppresses IRF3 nuclear translocation By similarity.
Subunit structure	Interacts (via C-terminus) with host IRF3; this interaction leads to IRF3 degradation. Interacts with host IRF7; this interaction leads to IRF7 degradation By similarity.
Subcellular location	Host cytoplasm › host cytoskeleton By similarity.
Domain	The zinc-finger domain is important, but not sufficient for binding and degrading IRF3. It is sometimes described as a RING zinc-finger, but it is atypical and it is unclear whether it is related with ubiquitin ligase activity By similarity.
Sequence similarities	Belongs to the rotavirus A NSP1 family.

Ontologies

Keywords
Biological process	Host-virus interaction Inhibition of host IFN-mediated response initiation by virus Inhibition of host IRF3 by virus Inhibition of host IRF7 by virus Inhibition of host NF-kappa-B by virus Inhibition of host innate immune response by virus Interferon antiviral system evasion Viral immunoevasion
Cellular component	Host cytoplasm Host cytoskeleton
Domain	Zinc-finger
Ligand	Metal-binding RNA-binding Zinc
Gene Ontology (GO)
Biological_process	suppression by virus of host IRF3 activity Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host IRF7 activity Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host NF-kappaB transcription factor activity Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host type I interferon production Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW host cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 486	486	Non-structural protein 1		PRO_0000149556
Regions
	Zinc finger	42 – 79	38	Potential
	Region	1 – 81	81	RNA-binding By similarity
	Region	82 – 176	95	Important for cytoskeleton localization By similarity
	Region	313 – 486	174	Interaction with IRF3 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P30212 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 6A9E6033A3488D2B	FASTA	486	57,645
	10 20 30 40 50 60 MATFKDACYH YKRINKLNHT VLKLGVNDTW RPSPPTKYKG WCLDCCQHTD LTYCRGCTMY 70 80 90 100 110 120 HVCQWCSQYG RCFLDNEPHL LRMRTFKNEV TKDDLKNLID MYETLFPMNQ RIVCRFINNT 130 140 150 160 170 180 RQHKCRNECM TQWYNHLLLP ITLQSMSIEL DGDVYYVFGY YDNMNSINQT PFSFTNLVDI 190 200 210 220 230 240 YDKLLLDDVN FTRMSFLPAS LQQEYALRYF SKSRFISEQR KCVNDSHFSI NVIENLHNPS 250 260 270 280 290 300 FKVQITRNCS ELSFDWNKAC KLVKKISAYF DILKTSHIEF YSVSTRCRIF TQCKLKMASK 310 320 330 340 350 360 LIKPNYITSN HKTLATEVHN CKWCSVNNSY TVWNDFRIKN IYDNIFNFLR ALVKSNVNIG 370 380 390 400 410 420 HCSSQEKIYE YVEDVLNVCD DERWKTSIME IFNCLEPVEL DDVKYVLLNH EINWDVINVL 430 440 450 460 470 480 VHSIGKVPQI LTLENVIAIM QSIIYEWFDI RYMRNTPMVT FTIDKLRRLH TGLKTVDYDS GISDIE

References

[1]	Kawagishi M., Matsuno S. Submitted (MAY-1991) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X59297 Genomic RNA. Translation: CAA41986.1.
PIR	S15470.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002148. Rotavirus_NSP1. [Graphical view]
Pfam	PF00981. Rota_NS53. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NSP1_ROTHI

Accession

Primary (citable) accession number: P30212

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	January 9, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program