Non-structural protein 1

Functionⁱ

Plays a role in the inhibition of host innate immunity by inducing the degradation of key host factors required to activate interferon production such as IRF3, IRF5 or IRF7. Associates with components of cullin RING ligases (CRLs) including CUL1 or CUL3, which are essential multisubunit ubiquitination complexes, to modulate their activities. Recognizes the host NF-kappa-B regulator BTRC through the presence of a DSGXS motif in the C-terminal substrate recognition domain.UniRule annotation

GO - Molecular functionⁱ

metal ion binding Source: UniProtKB-KW
RNA binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

suppression by virus of host IRF3 activity Source: UniProtKB-KW
suppression by virus of host IRF7 activity Source: UniProtKB-KW
suppression by virus of host NF-kappaB transcription factor activity Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	RNA-binding
Biological process	Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host IRF3 by virus, Inhibition of host IRF7 by virus, Inhibition of host NF-kappa-B by virus, Inhibition of host RLR pathway by virus, Interferon antiviral system evasion, Viral immunoevasion
Ligand	Metal-binding

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Non-structural protein 1UniRule annotation Short name: NSP1UniRule annotation Alternative name(s): NCVP2UniRule annotation Non-structural RNA-binding protein 53UniRule annotation Short name: NS53UniRule annotation
Organismⁱ	Rotavirus A (isolate RVA/Human/Japan/IGV-80-3/XXXX/GXP[X]) (RV-A)
Taxonomic identifierⁱ	31574 [NCBI]
Taxonomic lineageⁱ	Viruses › dsRNA viruses › Reoviridae › Sedoreovirinae › Rotavirus › Rotavirus A › Rotavirus A isolates
Virus hostⁱ	Homo sapiens (Human) [TaxID: 9606]

Subcellular locationⁱ

host cytoskeleton
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_04088

GO - Cellular componentⁱ

host cell cytoplasm Source: UniProtKB-KW
host cytoskeleton Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Host cytoplasm, Host cytoskeleton

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000149556	1 – 486	Non-structural protein 1Add BLAST		486

Post-translational modificationⁱ

The C-terminal region is phosphorylated by host CKII/CSNK2A1. Phosphorylation of the DSGXS motif is essential for host NF-kappa-B inhibition.UniRule annotation

Keywords - PTMⁱ

Phosphoprotein

Interactionⁱ

Subunit structureⁱ

Interacts (via C-terminus) with host IRF3; this interaction leads to IRF3 degradation. Interacts with host IRF7; this interaction leads to IRF7 degradation. Interacts with host CUL1 and CUL3. Interacts with host BTRC.UniRule annotation

Structureⁱ

3D structure databases

SMRⁱ	P30212.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	1 – 81	RNA-bindingUniRule annotationAdd BLAST	81
Regionⁱ	42 – 79	Zinc-binding domainUniRule annotationAdd BLAST	38
Regionⁱ	82 – 176	Important for cytoskeleton localizationUniRule annotationAdd BLAST	95
Regionⁱ	317 – 486	Interaction with host IRF3UniRule annotationAdd BLAST	170

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	479 – 483	IKBKB-like degron (ILD) motifUniRule annotation		5

Domainⁱ

The integrity of the zinc-binding domain in NSP1 is important for degradation of host IRF3.UniRule annotation

Sequence similaritiesⁱ

Belongs to the rotavirus NSP1 family.UniRule annotation

Family and domain databases

HAMAPⁱ	MF_04088. ROTA_NSP1. 1 hit.
InterProⁱ	View protein in InterPro IPR002148. Rotavirus_NSP1.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00981. Rota_NS53. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

P30212-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MATFKDACYH YKRINKLNHT VLKLGVNDTW RPSPPTKYKG WCLDCCQHTD 
        60         70         80         90        100
LTYCRGCTMY HVCQWCSQYG RCFLDNEPHL LRMRTFKNEV TKDDLKNLID 
       110        120        130        140        150
MYETLFPMNQ RIVCRFINNT RQHKCRNECM TQWYNHLLLP ITLQSMSIEL 
       160        170        180        190        200
DGDVYYVFGY YDNMNSINQT PFSFTNLVDI YDKLLLDDVN FTRMSFLPAS 
       210        220        230        240        250
LQQEYALRYF SKSRFISEQR KCVNDSHFSI NVIENLHNPS FKVQITRNCS 
       260        270        280        290        300
ELSFDWNKAC KLVKKISAYF DILKTSHIEF YSVSTRCRIF TQCKLKMASK 
       310        320        330        340        350
LIKPNYITSN HKTLATEVHN CKWCSVNNSY TVWNDFRIKN IYDNIFNFLR 
       360        370        380        390        400
ALVKSNVNIG HCSSQEKIYE YVEDVLNVCD DERWKTSIME IFNCLEPVEL 
       410        420        430        440        450
DDVKYVLLNH EINWDVINVL VHSIGKVPQI LTLENVIAIM QSIIYEWFDI 
       460        470        480 
RYMRNTPMVT FTIDKLRRLH TGLKTVDYDS GISDIE

Length:486

Mass (Da):57,645

Last modified:April 1, 1993 - v1

Checksum:ⁱ6A9E6033A3488D2B

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X59297 Genomic RNA. Translation: CAA41986.1.
PIRⁱ	S15470.

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
P30212	Q9QNA9 J9QNE1 J9QL90 X2C166 G3FZH2 J9QMH5 J9QMH1 O40627 Q9IUH7 B3SRV2 +244	Rotavirus A (strain RVA/Human/Japan/KU/1995/G1P1A[8]) (RV-A) Human rotavirus A Rotavirus A (strain RVA/Human/Japan/K8/1977/G1P3A[9]) (RV-A) Rotavirus A Rotavirus A (strain RVA/Human/United States/P/1974/G3P1A[8]) (RV-A) Rotavirus A human/Bethesda/DC1563/1974/G3P[8] Rotavirus A human/Vanderbilt/VU05-06-26/2005/G1P[8] Rotavirus A human/Vanderbilt/VU06-07-33/2006/G1P[8] Rotavirus A human/Vanderbilt/VU05-06-27/2005/G1P[8] Rotavirus A human/Victoria/CK00045/2006/G1P[8] And more	486	UniRef90_Q9QNA9	Cluster: Non-structural protein 1	255

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
P30212	Q8JZ13 A0A059UKQ5 A0A1D8MAJ1 B3SRR2 X4YML9 P12475 W0G1J0 A0A142I7Y5 A0A1B4XK28 A0A077KA21 +1988	Rotavirus A (strain RVA/Cow/United States/B641/XXXX/G6P7[5]) (RV-A) Rotavirus A Rotavirus A (isolate RVA/Human/United Kingdom/A64/1987/G10P11[14]) (RV-A) Rotavirus A (strain RVA/Cow/France/RF/1975/G6P6[1]) (RV-A) Rotavirus A RVA/Cow-tc/THA/61A/1989/G10P[5] Porcine rotavirus A Rotavirus A (strain RVA/Cow/United States/WC3/1981/G6P7[5]) (RV-A) (Rotavirus (strain Wistar calf 3)) Human rotavirus A Rotavirus A giraffe/UCD/IRL/2007 Rotavirus A (isolate RVA/Cow/Thailand/A44/1988/G10P8[11]) (RV-A) And more	491	UniRef50_Q8JZ13	Cluster: Non-structural protein 1	1,999

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X59297 Genomic RNA. Translation: CAA41986.1.
PIRⁱ	S15470.

3D structure databases

SMRⁱ	P30212.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Family and domain databases

HAMAPⁱ	MF_04088. ROTA_NSP1. 1 hit.
InterProⁱ	View protein in InterPro IPR002148. Rotavirus_NSP1.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00981. Rota_NS53. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	NSP1_ROTHI
Accessionⁱ	P30212Primary (citable) accession number: P30212
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
	Last sequence update:	April 1, 1993
	Last modified:	March 28, 2018
	This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Viral Protein Annotation Program

UniProtKB - P30212 (NSP1_ROTHI)

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Keywordsⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Molecule processing

Post-translational modificationⁱ

Keywords - PTMⁱ

Interactionⁱ

Subunit structureⁱ

Structureⁱ

3D structure databases

Family & Domainsⁱ

Region

Motif

Domainⁱ

Sequence similaritiesⁱ

Family and domain databases

Sequenceⁱ

Sequence databases

Similar proteinsⁱ

Cross-referencesⁱ

Sequence databases

3D structure databases

Protocols and materials databases

Family and domain databases

Entry informationⁱ

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P30212 (NSP1_ROTHI)

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Non-structural protein 1

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

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<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Family and domain databases

Sequence databases

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Sequence databases

3D structure databases

Protocols and materials databases

Family and domain databases

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ