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P30211

- SIGM3_REOVJ

UniProt

P30211 - SIGM3_REOVJ

Protein

Outer capsid protein sigma-3

Gene

S4

Organism
Reovirus type 2 (strain D5/Jones) (T2J) (Mammalian orthoreovirus 2)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Stimulates translation by blocking the activation of the dsRNA-dependent protein kinase EIF2AK2/PKR, thereby inhibiting the host interferon response. Sigma3 prevents the activation of EIF2AK2 by competing with the kinase for dsRNA-binding By similarity.By similarity
    The viral outer shell polypeptides, of which sigma-3 is one, impose structural constraints that prevent elongation of nascent transcripts by the RNA-dependent RNA polymerase lambda-3.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri51 – 7323CCHC-typeBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. RNA binding Source: UniProtKB-KW
    3. structural molecule activity Source: InterPro

    GO - Biological processi

    1. regulation of transcription, DNA-templated Source: UniProtKB-KW
    2. regulation of translation Source: UniProtKB-KW
    3. suppression by virus of host PKR activity Source: UniProtKB-KW
    4. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
    5. transcription, DNA-templated Source: UniProtKB-KW
    6. viral life cycle Source: InterPro

    Keywords - Biological processi

    Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host PKR by virus, Interferon antiviral system evasion, Transcription, Transcription regulation, Translation regulation, Viral immunoevasion

    Keywords - Ligandi

    Metal-binding, RNA-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Outer capsid protein sigma-3
    Short name:
    Sigma3
    Gene namesi
    Name:S4
    OrganismiReovirus type 2 (strain D5/Jones) (T2J) (Mammalian orthoreovirus 2)
    Taxonomic identifieri10885 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
    Virus hostiMammalia [TaxID: 40674]
    ProteomesiUP000006370: Genome

    Subcellular locationi

    Virion By similarity
    Note: Found in the outer capsid. Each subunit is positioned with the small lobe anchoring it to the protein mu1 on the surface of the virion, and the large lobe, the site of initial cleavages during entry-related proteolytic disassembly, protruding outwards By similarity.By similarity

    GO - Cellular componenti

    1. viral outer capsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Outer capsid protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 365365Outer capsid protein sigma-3PRO_0000222753Add
    BLAST

    Post-translational modificationi

    Cleaved during virus the endosomal proteolytic disassembly of the outer capsid.By similarity

    Interactioni

    Subunit structurei

    Heterohexamer of three sigma-3 and three Mu-1 proteins. The RNA-binding form is probably a homodimer By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP30211.
    SMRiP30211. Positions 1-365.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Contains 1 CCHC-type zinc finger.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri51 – 7323CCHC-typeBy similarityAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Family and domain databases

    InterProiIPR000153. Reo_capsid_sigma3.
    IPR023634. Reovirus_capsid_sigma-3_dom.
    [Graphical view]
    PfamiPF00979. Reovirus_cap. 1 hit.
    [Graphical view]
    SUPFAMiSSF64465. SSF64465. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P30211-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEVCLPNGHQ IVDWINNAFE GRVSIYSAQQ GWDKTISAQP DMMVCGGAVV    50
    CMHCLGVVGS LQRKLKHLPH HKCNQQLRQQ DYVDVQFADR VTAHWKRGML 100
    SFVSQMHAIM NDVTPEELER VRTDGGSLAE LNWLQVDPGS MFRSIHSSWT 150
    DPLQVVEDLD TQLDRYWTAL NLMIDSSDLV PNFMMRDPSH AFNGVKLEGE 200
    ARQTQFSRTF DSRSNLEWGV MIYDYSELER DPLKGRAYRK EVVTPARDFG 250
    HFGLSHYSRA TTPILGKMPA VFSGMLTGNC KMYPFIKGTA KLRTVKKLVD 300
    AVNHTWGSEK IRYALGPGGM TGWYNRTMQQ APIVLTPAAL TMFPDMTKFG 350
    DLQYPIMIGD PAVLG 365
    Length:365
    Mass (Da):41,226
    Last modified:April 1, 1993 - v1
    Checksum:i574350AEDFE04478
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X60066 mRNA. Translation: CAA42670.1.
    PIRiB42192. MNXRSJ.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X60066 mRNA. Translation: CAA42670.1 .
    PIRi B42192. MNXRSJ.

    3D structure databases

    ProteinModelPortali P30211.
    SMRi P30211. Positions 1-365.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR000153. Reo_capsid_sigma3.
    IPR023634. Reovirus_capsid_sigma-3_dom.
    [Graphical view ]
    Pfami PF00979. Reovirus_cap. 1 hit.
    [Graphical view ]
    SUPFAMi SSF64465. SSF64465. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Translational effects and sequence comparisons of the three serotypes of the reovirus S4 gene."
      Seliger L.S., Giantini M., Shatkin A.J.
      Virology 187:202-210(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiSIGM3_REOVJ
    AccessioniPrimary (citable) accession number: P30211
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3