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Protein

Outer capsid protein sigma-3

Gene

S4

Organism
Reovirus type 2 (strain D5/Jones) (T2J) (Mammalian orthoreovirus 2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Stimulates translation by blocking the activation of the dsRNA-dependent protein kinase EIF2AK2/PKR, thereby inhibiting the host interferon response. Sigma3 prevents the activation of EIF2AK2 by competing with the kinase for dsRNA-binding (By similarity).By similarity
The viral outer shell polypeptides, of which sigma-3 is one, impose structural constraints that prevent elongation of nascent transcripts by the RNA-dependent RNA polymerase lambda-3.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri51 – 7323CCHC-typeBy similarityAdd
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW
  3. structural molecule activity Source: InterPro

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: UniProtKB-KW
  2. regulation of translation Source: UniProtKB-KW
  3. suppression by virus of host PKR activity Source: UniProtKB-KW
  4. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
  5. transcription, DNA-templated Source: UniProtKB-KW
  6. viral life cycle Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host PKR by virus, Interferon antiviral system evasion, Transcription, Transcription regulation, Translation regulation, Viral immunoevasion

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Outer capsid protein sigma-3
Short name:
Sigma3
Gene namesi
Name:S4
OrganismiReovirus type 2 (strain D5/Jones) (T2J) (Mammalian orthoreovirus 2)
Taxonomic identifieri10885 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
Virus hostiMammalia [TaxID: 40674]
ProteomesiUP000006370: Genome

Subcellular locationi

Virion By similarity
Note: Found in the outer capsid. Each subunit is positioned with the small lobe anchoring it to the protein mu1 on the surface of the virion, and the large lobe, the site of initial cleavages during entry-related proteolytic disassembly, protruding outwards (By similarity).By similarity

GO - Cellular componenti

  1. viral outer capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Outer capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 365365Outer capsid protein sigma-3PRO_0000222753Add
BLAST

Post-translational modificationi

Cleaved during virus the endosomal proteolytic disassembly of the outer capsid.By similarity

Interactioni

Subunit structurei

Heterohexamer of three sigma-3 and three Mu-1 proteins. The RNA-binding form is probably a homodimer (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP30211.
SMRiP30211. Positions 1-365.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 CCHC-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri51 – 7323CCHC-typeBy similarityAdd
BLAST

Keywords - Domaini

Zinc-finger

Family and domain databases

InterProiIPR000153. Reo_capsid_sigma3.
IPR023634. Reovirus_capsid_sigma-3_dom.
[Graphical view]
PfamiPF00979. Reovirus_cap. 1 hit.
[Graphical view]
SUPFAMiSSF64465. SSF64465. 1 hit.

Sequencei

Sequence statusi: Complete.

P30211-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVCLPNGHQ IVDWINNAFE GRVSIYSAQQ GWDKTISAQP DMMVCGGAVV
60 70 80 90 100
CMHCLGVVGS LQRKLKHLPH HKCNQQLRQQ DYVDVQFADR VTAHWKRGML
110 120 130 140 150
SFVSQMHAIM NDVTPEELER VRTDGGSLAE LNWLQVDPGS MFRSIHSSWT
160 170 180 190 200
DPLQVVEDLD TQLDRYWTAL NLMIDSSDLV PNFMMRDPSH AFNGVKLEGE
210 220 230 240 250
ARQTQFSRTF DSRSNLEWGV MIYDYSELER DPLKGRAYRK EVVTPARDFG
260 270 280 290 300
HFGLSHYSRA TTPILGKMPA VFSGMLTGNC KMYPFIKGTA KLRTVKKLVD
310 320 330 340 350
AVNHTWGSEK IRYALGPGGM TGWYNRTMQQ APIVLTPAAL TMFPDMTKFG
360
DLQYPIMIGD PAVLG
Length:365
Mass (Da):41,226
Last modified:April 1, 1993 - v1
Checksum:i574350AEDFE04478
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60066 mRNA. Translation: CAA42670.1.
PIRiB42192. MNXRSJ.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60066 mRNA. Translation: CAA42670.1.
PIRiB42192. MNXRSJ.

3D structure databases

ProteinModelPortaliP30211.
SMRiP30211. Positions 1-365.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR000153. Reo_capsid_sigma3.
IPR023634. Reovirus_capsid_sigma-3_dom.
[Graphical view]
PfamiPF00979. Reovirus_cap. 1 hit.
[Graphical view]
SUPFAMiSSF64465. SSF64465. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Translational effects and sequence comparisons of the three serotypes of the reovirus S4 gene."
    Seliger L.S., Giantini M., Shatkin A.J.
    Virology 187:202-210(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiSIGM3_REOVJ
AccessioniPrimary (citable) accession number: P30211
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: January 7, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.