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Protein

DNA topoisomerase 1

Gene

Top1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex (By similarity). Introduces a single-strand break via transesterification at a target site in duplex DNA (By similarity). The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand (By similarity). The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils (By similarity). Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).By similarity

Miscellaneous

Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei930O-(3'-phospho-DNA)-tyrosine intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • DNA topoisomerase activity Source: FlyBase
  • DNA topoisomerase type I activity Source: FlyBase

GO - Biological processi

  • cell proliferation Source: FlyBase
  • chromatin remodeling Source: GO_Central
  • chromosome condensation Source: FlyBase
  • chromosome segregation Source: FlyBase
  • DNA replication Source: GO_Central
  • DNA topological change Source: FlyBase
  • instar larval development Source: FlyBase
  • multicellular organism development Source: FlyBase
  • oogenesis Source: FlyBase
  • regulation of embryonic development Source: FlyBase

Keywordsi

Molecular functionDNA-binding, Isomerase, Topoisomerase

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase I
Gene namesi
Name:Top1Imported
ORF Names:CG6146Imported
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0004924 Top1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001452051 – 972DNA topoisomerase 1Add BLAST972

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei303Phosphoserine1 Publication1
Modified residuei304Phosphotyrosine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP30189
PRIDEiP30189

PTM databases

iPTMnetiP30189

Expressioni

Developmental stagei

Detected in germline and follicle cells. Expressed in follicle cells throughout oogenesis. In germline cells, expression levels increase as cells develop and move to the posterior region of the germarium, then at stage 5 of egg chamber development levels decrease until it is no longer detected (at protein level).1 Publication

Gene expression databases

BgeeiFBgn0004924
ExpressionAtlasiP30189 baseline and differential
GenevisibleiP30189 DM

Interactioni

Subunit structurei

Interacts with Topors.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei539Interaction with DNABy similarity1
Sitei587Interaction with DNABy similarity1
Sitei635Interaction with DNABy similarity1
Sitei666Interaction with DNABy similarity1
Sitei723Interaction with DNABy similarity1
Sitei754Interaction with DNABy similarity1
Sitei796Interaction with DNABy similarity1
Sitei854Interaction with DNABy similarity1
Sitei872Interaction with DNABy similarity1

Protein-protein interaction databases

BioGridi58813, 9 interactors
IntActiP30189, 1 interactor
STRINGi7227.FBpp0073822

Structurei

3D structure databases

ProteinModelPortaliP30189
SMRiP30189
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni648 – 649Interaction with DNABy similarity2
Regioni711 – 716Interaction with DNABy similarity6
Regioni807 – 809Interaction with DNABy similarity3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi32 – 39Poly-His8
Compositional biasi40 – 198Ser-richAdd BLAST159

Sequence similaritiesi

Belongs to the type IB topoisomerase family.Curated

Phylogenomic databases

eggNOGiKOG0981 Eukaryota
COG3569 LUCA
InParanoidiP30189
KOiK03163
OrthoDBiEOG091G02KT
PhylomeDBiP30189

Family and domain databases

CDDicd00659 Topo_IB_C, 1 hit
Gene3Di1.10.10.41, 1 hit
1.10.132.10, 1 hit
2.170.11.10, 2 hits
3.90.15.10, 1 hit
InterProiView protein in InterPro
IPR011010 DNA_brk_join_enz
IPR013034 DNA_topo_DNA_db_N_dom1
IPR013030 DNA_topo_DNA_db_N_dom2
IPR001631 TopoI
IPR018521 TopoI_AS
IPR025834 TopoI_C_dom
IPR014711 TopoI_cat_a-hlx-sub_euk
IPR014727 TopoI_cat_a/b-sub_euk
IPR013500 TopoI_cat_euk
IPR008336 TopoI_DNA-bd_euk
IPR036202 TopoI_DNA-bd_euk_N_sf
IPR013499 TopoI_euk
PANTHERiPTHR10290 PTHR10290, 1 hit
PfamiView protein in Pfam
PF14370 Topo_C_assoc, 1 hit
PF01028 Topoisom_I, 1 hit
PF02919 Topoisom_I_N, 1 hit
PRINTSiPR00416 EUTPISMRASEI
SMARTiView protein in SMART
SM00435 TOPEUc, 1 hit
SUPFAMiSSF56349 SSF56349, 2 hits
SSF56741 SSF56741, 1 hit
PROSITEiView protein in PROSITE
PS00176 TOPOISOMERASE_I_EUK, 1 hit

Sequencei

Sequence statusi: Complete.

P30189-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGDVAAENS IHIQNGGSCE VVQSNGVTTN GHGHHHHHHS SSSSSSKHKS
60 70 80 90 100
SSKDKHRDRE REHKSSNSSS SSKEHKSSSR DKDRHKSSSS SSKHRDKDKE
110 120 130 140 150
RDGSSNSHRS GSSSSHKDKD GSSSSKHKSS SGHHKRSSKD KERRDKDKDR
160 170 180 190 200
GSSSSSRHKS SSSSRDKERS SSSHKSSSSS SSSKSKHSSS RHSSSSSSKD
210 220 230 240 250
HPSYDGVFVK PEPVSQQLMH SGSVDAFQMQ QLGSYEAAAA GTNFNGNGNV
260 270 280 290 300
AGANYKNGYE ESIVDIKKEE ESFNNLSQAS SCDYSMSQFR ADEPPFVVKH
310 320 330 340 350
EQSYAEEDST MNYNDHDDDA DEMNDDEEDV PLAMRKRKQE ATDRPDGGMD
360 370 380 390 400
NDDDDDIPLL ARKKVKKEKI KKESKEKSKK RVKEEPSDDY GNVKPKKKKM
410 420 430 440 450
KKEPEPAVSP GKRQKAKAKV EEEEVWRWWE EEKRADGVKW STLEHKGPVF
460 470 480 490 500
APRYERVPRN VRFYYDGKPL ELSEETEEAA TFYAKMLNHD YCTKEVFNNN
510 520 530 540 550
FFKDFRKSMT PREREIIKDF RKCNFQEMFN YFQAESEKRK AASKEEKLIK
560 570 580 590 600
KNENEALMKE FGFCMIDGHK EKIGNFRLEP PGLFRGRGEH PKMGMIKRRI
610 620 630 640 650
QASDVSINCG KDSKVPSPPP GSRWKEVRHD NTVTWLASWI ENVQGQVKYI
660 670 680 690 700
MLNPSSKLKG EKDHIKYETA RRLDKVIDKI RATYRDEWKS KEMRVRQRAV
710 720 730 740 750
ALYFIDKLAL RAGNEKDEDQ ADTVGCCSLR VEHVQLHKEL NGKENVVVFD
760 770 780 790 800
FPGKDSIRYY NEVEVEKRVF KNLELFMEHK KEGDDLFDRL NTQVLNEHLK
810 820 830 840 850
ELMEGLTAKV FRTYNASKTL QSQLDLLTDP SATVPEKLLA YNRANRAVAI
860 870 880 890 900
LCNHQRSVPK SHEKSMENLK EKIKAKREAI EKCESEYHSR DEKKGKQLER
910 920 930 940 950
LRDQLKKLEL QETDRDENKT IALGTSKLNY LDPRISVAWC KKHDVPIEKI
960 970
FNKTQRTKFL WAVHMADENY RF
Length:972
Mass (Da):111,688
Last modified:April 1, 1993 - v1
Checksum:i3764B8BDEEFA30CD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti40S → H in AAF48440 (PubMed:10731132).Curated1
Sequence conflicti46S → SSS in AAF48440 (PubMed:10731132).Curated1
Sequence conflicti201H → Q in AAF48440 (PubMed:10731132).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74557 mRNA Translation: AAA28951.1
U80064 Genomic DNA Translation: AAC24158.1
AE014298 Genomic DNA Translation: AAF48440.1
PIRiS35521
RefSeqiNP_001014742.1, NM_001014742.2
NP_511161.2, NM_078606.4
UniGeneiDm.2912

Genome annotation databases

GeneIDi32458
KEGGidme:Dmel_CG6146

Similar proteinsi

Entry informationi

Entry nameiTOP1_DROME
AccessioniPrimary (citable) accession number: P30189
Secondary accession number(s): Q9VXW6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: May 23, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

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