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Protein

Proteasome subunit alpha type-7-A

Gene

PAD1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the association of the SCF(TIR1) E3 ubiquitin ligase complex with the proteasome.1 Publication

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiR-ATH-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-ATH-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-ATH-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-ATH-5632684 Hedgehog 'on' state
R-ATH-5687128 MAPK6/MAPK4 signaling
R-ATH-5689603 UCH proteinases
R-ATH-5689880 Ub-specific processing proteases
R-ATH-68949 Orc1 removal from chromatin
R-ATH-69017 CDK-mediated phosphorylation and removal of Cdc6
R-ATH-69229 Ubiquitin-dependent degradation of Cyclin D1
R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit alpha type-7-A (EC:3.4.25.1)
Alternative name(s):
20S proteasome alpha subunit D-1
Proteasome component 6A
Proteasome subunit alpha type-4
TAS-G64
Gene namesi
Name:PAD1
Synonyms:PRC6A
Ordered Locus Names:At3g51260
ORF Names:F24M12.300
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G51260
TAIRilocus:2080833 AT3G51260

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001241591 – 250Proteasome subunit alpha type-7-AAdd BLAST250

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki62Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiP30186
PRIDEiP30186

Expressioni

Tissue specificityi

Expressed in roots, leaves and flowers.1 Publication

Inductioni

During cell proliferation.1 Publication

Gene expression databases

ExpressionAtlasiP30186 baseline and differential
GenevisibleiP30186 AT

Interactioni

Subunit structurei

Component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Interacts with KIN10 and KIN11 SnRK subunits, and with the SKP1A/ASK1 subunit of the SCF E3 ubiquitin ligase complex.4 Publications

Protein-protein interaction databases

BioGridi9607, 8 interactors
IntActiP30186, 4 interactors
STRINGi3702.AT3G51260.1

Structurei

3D structure databases

ProteinModelPortaliP30186
SMRiP30186
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1A family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0183 Eukaryota
ENOG410XP21 LUCA
HOGENOMiHOG000091085
InParanoidiP30186
KOiK02731
OMAiPYTQKPA
OrthoDBiEOG09360M33
PhylomeDBiP30186

Family and domain databases

Gene3Di3.60.20.10, 1 hit
InterProiView protein in InterPro
IPR029055 Ntn_hydrolases_N
IPR023332 Proteasome_alpha-type
IPR000426 Proteasome_asu_N
IPR001353 Proteasome_sua/b
PfamiView protein in Pfam
PF00227 Proteasome, 1 hit
PF10584 Proteasome_A_N, 1 hit
SMARTiView protein in SMART
SM00948 Proteasome_A_N, 1 hit
SUPFAMiSSF56235 SSF56235, 1 hit
PROSITEiView protein in PROSITE
PS00388 PROTEASOME_ALPHA_1, 1 hit
PS51475 PROTEASOME_ALPHA_2, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P30186-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARYDRAITV FSPDGHLFQV EYALEAVRKG NAAVGVRGTD TVVLAVEKKS
60 70 80 90 100
TPKLQDSRSA RKIVSLDNHI ALACAGLKAD ARVLINKARI ECQSHRLTLE
110 120 130 140 150
DPVTVEYITR YIAGLQQKYT QSGGVRPFGL STLIVGFDPY TRIPALYQTD
160 170 180 190 200
PSGTFSAWKA NATGRNSNSI REFLEKNYKE SAGQETVKLA IRALLEVVES
210 220 230 240 250
GGKNIEVAVM TREEGVLKQL EEEEIDIIVA EIEAEKAAAE AAKKGPAKET
Length:250
Mass (Da):27,337
Last modified:April 1, 1993 - v1
Checksum:iE026335F50C74AA3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti15G → N in CAA79082 (Ref. 7) Curated1
Sequence conflicti27V → L in AAM64989 (Ref. 6) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66825 mRNA Translation: CAA47298.1
AF043522 mRNA Translation: AAC32058.1
AL132980 Genomic DNA Translation: CAB62648.1
CP002686 Genomic DNA Translation: AEE78769.1
AY042820 mRNA Translation: AAK68760.1
AY052321 mRNA Translation: AAK96514.1
AY061899 mRNA Translation: AAL31226.1
AY081448 mRNA Translation: AAM10010.1
AY087443 mRNA Translation: AAM64989.1
Z17987 mRNA Translation: CAA79082.1
PIRiS29240
RefSeqiNP_190694.1, NM_114985.3 [P30186-1]
UniGeneiAt.19974
At.67272

Genome annotation databases

EnsemblPlantsiAT3G51260.1; AT3G51260.1; AT3G51260 [P30186-1]
GeneIDi824289
GrameneiAT3G51260.1; AT3G51260.1; AT3G51260 [P30186-1]
KEGGiath:AT3G51260

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPSA7A_ARATH
AccessioniPrimary (citable) accession number: P30186
Secondary accession number(s): Q41942, Q8LB36
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: May 23, 2018
This is version 154 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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