Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA topoisomerase 2

Gene

TOP2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.PROSITE-ProRule annotation

Cofactori

Mg2+PROSITE-ProRule annotation, Mn2+PROSITE-ProRule annotation, Ca2+PROSITE-ProRule annotationNote: Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ or Ca2+.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei91ATPBy similarity1
Binding sitei120ATPBy similarity1
Metal bindingi461Magnesium 1; catalyticPROSITE-ProRule annotation1
Metal bindingi538Magnesium 1; catalyticPROSITE-ProRule annotation1
Metal bindingi538Magnesium 2PROSITE-ProRule annotation1
Metal bindingi540Magnesium 2PROSITE-ProRule annotation1
Sitei793Transition state stabilizerBy similarity1
Active sitei794O-(5'-phospho-DNA)-tyrosine intermediateBy similarity1
Sitei845Important for DNA bending; intercalates between base pairs of target DNABy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi148 – 150ATPBy similarity3
Nucleotide bindingi161 – 168ATPBy similarity8
Nucleotide bindingi378 – 380ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

ATP-binding, DNA-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-4615885. SUMOylation of DNA replication proteins.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 2 (EC:5.99.1.3)
Alternative name(s):
DNA topoisomerase II
Gene namesi
Name:TOP2
Ordered Locus Names:At3g23890
ORF Names:F14O13.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G23890.

Subcellular locationi

GO - Cellular componenti

  • DNA topoisomerase complex (ATP-hydrolyzing) Source: GO_Central
  • nucleus Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001453801 – 1473DNA topoisomerase 2Add BLAST1473

Proteomic databases

PaxDbiP30182.
PRIDEiP30182.

PTM databases

iPTMnetiP30182.

Expressioni

Gene expression databases

ExpressionAtlasiP30182. baseline and differential.
GenevisibleiP30182. AT.

Interactioni

Subunit structurei

Homodimer.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei489Interaction with DNAPROSITE-ProRule annotation1
Sitei492Interaction with DNAPROSITE-ProRule annotation1
Sitei662Interaction with DNAPROSITE-ProRule annotation1
Sitei663Interaction with DNAPROSITE-ProRule annotation1
Sitei712Interaction with DNAPROSITE-ProRule annotation1
Sitei746Interaction with DNAPROSITE-ProRule annotation1
Sitei752Interaction with DNAPROSITE-ProRule annotation1
Sitei924Interaction with DNABy similarity1

Protein-protein interaction databases

STRINGi3702.AT3G23890.1.

Structurei

3D structure databases

ProteinModelPortaliP30182.
SMRiP30182.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini455 – 569ToprimPROSITE-ProRule annotationAdd BLAST115

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni345 – 347Interaction with DNABy similarity3
Regioni980 – 989Interaction with DNABy similarity10

Sequence similaritiesi

Belongs to the type II topoisomerase family.Curated
Contains 1 Toprim domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0355. Eukaryota.
COG0187. LUCA.
COG0188. LUCA.
HOGENOMiHOG000216693.
InParanoidiP30182.
KOiK03164.
OMAiTCSPDPR.
OrthoDBiEOG093600GF.
PhylomeDBiP30182.

Family and domain databases

Gene3Di1.10.268.10. 1 hit.
3.30.1360.40. 1 hit.
3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
3.90.199.10. 1 hit.
InterProiIPR024946. Arg_repress_C-like.
IPR003594. HATPase_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013760. Topo_IIA-like_dom.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013757. Topo_IIA_A_a.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR018522. TopoIIA_CS.
IPR031660. TOPRIM_C.
IPR006171. Toprim_domain.
[Graphical view]
PfamiPF00204. DNA_gyraseB. 1 hit.
PF00521. DNA_topoisoIV. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
PF16898. TOPRIM_C. 1 hit.
[Graphical view]
PRINTSiPR00418. TPI2FAMILY.
SMARTiSM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
SM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
PROSITEiPS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P30182-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATKLPLQNS NAANVAKAPA KSRAAAGGKT IEEMYQKKSQ LEHILLRPDT
60 70 80 90 100
YIGSIEKHTQ TLWVYEKDEM VQRPVTYVPG LYKIFDEILV NAADNKQRDA
110 120 130 140 150
KMDSVQVVID VEQNLISVCN SGAGVPVEIH QEEGIYVPEM IFGHLLTSSN
160 170 180 190 200
YDDNVKKTTG GRNGYGAKLT NIFSTEFIIE TADGKRLKKY KQVFENNMGK
210 220 230 240 250
KSEPVITKCN KSENWTKVTF KPDLKKFNMT ELEDDVVALM SKRVFDIAGC
260 270 280 290 300
LGKSVKVELN GKQIPVKSFT DYVDLYLSAA NKSRTEDPLP RLTEKVNDRW
310 320 330 340 350
EVCVSLSEGQ FQQVSFVNSI ATIKGGTHVD YVTSQITNHI VAAVNKKNKN
360 370 380 390 400
ANVKAHNVKN HLWVFVNALI DNPAFDSQTK ETLTLRQSSF GSKCELSEDF
410 420 430 440 450
LKKVGKSGVV ENLLSWADFK QNKDLKKSDG AKTGRVLVEK LDDAAEAGGK
460 470 480 490 500
NSRLCTLILT EGDSAKSLAL AGRSVLGNNY CGVFPLRGKL LNVREASTTQ
510 520 530 540 550
ITNNKEIENL KKILGLKQNM KYENVNSLRY GQMMIMTDQD HDGSHIKGLL
560 570 580 590 600
INFIHSFWPS LLQVPSFLVE FITPIVKATR KGTKKVLSFY SMPEYEEWKE
610 620 630 640 650
SLKGNATGWD IKYYKGLGTS TAEEGKEYFS NLGLHKKDFV WEDEQDGEAI
660 670 680 690 700
ELAFSKKKIE ARKNWLSSYV PGNHLDQRQP KVTYSDFVNK ELILFSMADL
710 720 730 740 750
QRSIPSMVDG LKPGQRKILF VAFKKIARKE MKVAQLVGYV SLLSAYHHGE
760 770 780 790 800
QSLASAIIGM AQDYVGSNNI NLLLPNGQFG TRTSGGKDSA SARYIFTKLS
810 820 830 840 850
PVTRILFPKD DDLLLDYLNE DGQRIEPTWY MPIIPTVLVN GAEGIGTGWS
860 870 880 890 900
TFIPNYNPRE IVANVRRLLN GESMVPMDPW YRGFKGTIEK TASKEGGCTY
910 920 930 940 950
TITGLYEEVD ETTIRITELP IRRWNDDYKN FLQSLKTDNG APFFQDVKAY
960 970 980 990 1000
NDEKSVDFDL ILSEENMLAA RQEGFLKKFK LTTTIATSNM HLFDKKGVIK
1010 1020 1030 1040 1050
KYVTPEQILE EFFDLRFEYY EKRKETVVKN MEIELLKLEN KARFILAVLS
1060 1070 1080 1090 1100
GEIIVNKRKK ADIVEDLRQK GFTPFPRKAE SVEAAIAGAV DDDAAEEPEE
1110 1120 1130 1140 1150
ILVDPESSSS YIPGSEYDYL LAMAIASLTI EKVEELLADR DKMIIAVADM
1160 1170 1180 1190 1200
KKTTPKSLWL SDLESLDKEL EKLDLKDAQV QQAIEAAQKK IRAKSGAAVK
1210 1220 1230 1240 1250
VKRQAPKKPA PKKTTKKASE SETTEASYSA MDTDNNVAEV VKPKARQGAK
1260 1270 1280 1290 1300
KKASESETTE ASHSAMDTDN NVAEVVKPKG RQGAKKKAPA AAKEVEEDEM
1310 1320 1330 1340 1350
LDLAQRLAQY NFGSAPADSS KTAETSKAIA VDDDDDDVVV EVAPVKKGGR
1360 1370 1380 1390 1400
KPAATKAAKP PAAPRKRGKQ TVASTEVLAI GVSPEKKVRK MRSSPFNKKS
1410 1420 1430 1440 1450
SSVMSRLADN KEEESSENVA GNSSSEKSGG DVSAISRPQR ANRRKMTYVL
1460 1470
SDSESESAND SEFDDIEDDE DDE
Length:1,473
Mass (Da):164,107
Last modified:November 1, 1995 - v2
Checksum:i00B6C4836E381403
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti1213K → N in strain: cv. Kas-1. 1
Natural varianti1245A → G in strain: cv. Kas-1. 1
Natural varianti1299E → G in strain: cv. Kas-1. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L21015 mRNA. Translation: AAA65448.1.
AP001297 Genomic DNA. Translation: BAB03006.1.
CP002686 Genomic DNA. Translation: AEE76826.1.
U12284 Genomic DNA. Translation: AAC48999.1.
U12285 Genomic DNA. Translation: AAC49000.1.
M84654 Genomic DNA. Translation: AAA32877.1.
PIRiS53598. S53599.
RefSeqiNP_189031.1. NM_113294.3. [P30182-1]
UniGeneiAt.22343.

Genome annotation databases

EnsemblPlantsiAT3G23890.1; AT3G23890.1; AT3G23890. [P30182-1]
GeneIDi821972.
GrameneiAT3G23890.1; AT3G23890.1; AT3G23890.
KEGGiath:AT3G23890.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L21015 mRNA. Translation: AAA65448.1.
AP001297 Genomic DNA. Translation: BAB03006.1.
CP002686 Genomic DNA. Translation: AEE76826.1.
U12284 Genomic DNA. Translation: AAC48999.1.
U12285 Genomic DNA. Translation: AAC49000.1.
M84654 Genomic DNA. Translation: AAA32877.1.
PIRiS53598. S53599.
RefSeqiNP_189031.1. NM_113294.3. [P30182-1]
UniGeneiAt.22343.

3D structure databases

ProteinModelPortaliP30182.
SMRiP30182.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G23890.1.

PTM databases

iPTMnetiP30182.

Proteomic databases

PaxDbiP30182.
PRIDEiP30182.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G23890.1; AT3G23890.1; AT3G23890. [P30182-1]
GeneIDi821972.
GrameneiAT3G23890.1; AT3G23890.1; AT3G23890.
KEGGiath:AT3G23890.

Organism-specific databases

TAIRiAT3G23890.

Phylogenomic databases

eggNOGiKOG0355. Eukaryota.
COG0187. LUCA.
COG0188. LUCA.
HOGENOMiHOG000216693.
InParanoidiP30182.
KOiK03164.
OMAiTCSPDPR.
OrthoDBiEOG093600GF.
PhylomeDBiP30182.

Enzyme and pathway databases

ReactomeiR-ATH-4615885. SUMOylation of DNA replication proteins.

Miscellaneous databases

PROiP30182.

Gene expression databases

ExpressionAtlasiP30182. baseline and differential.
GenevisibleiP30182. AT.

Family and domain databases

Gene3Di1.10.268.10. 1 hit.
3.30.1360.40. 1 hit.
3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
3.90.199.10. 1 hit.
InterProiIPR024946. Arg_repress_C-like.
IPR003594. HATPase_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013760. Topo_IIA-like_dom.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013757. Topo_IIA_A_a.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR018522. TopoIIA_CS.
IPR031660. TOPRIM_C.
IPR006171. Toprim_domain.
[Graphical view]
PfamiPF00204. DNA_gyraseB. 1 hit.
PF00521. DNA_topoisoIV. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
PF16898. TOPRIM_C. 1 hit.
[Graphical view]
PRINTSiPR00418. TPI2FAMILY.
SMARTiSM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
SM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
PROSITEiPS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTOP2_ARATH
AccessioniPrimary (citable) accession number: P30182
Secondary accession number(s): Q38807
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.