DNA topoisomerase 1 - Arabidopsis thaliana (Mouse-ear cress)

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P30181 (TOP1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 84. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
DNA topoisomerase 1
EC=5.99.1.2

Alternative name(s):
DNA topoisomerase I

Gene names

Name:	TOP1
Ordered Locus Names:	At5g55300
ORF Names:	MTE17.1

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	916 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Subcellular location	Nucleus Ref.5.
Miscellaneous	Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.
Sequence similarities	Belongs to the type IB topoisomerase family.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro chromatin remodeling Inferred from genetic interaction. Source: TAIR embryo development ending in seed dormancy Inferred from mutant phenotype. Source: TAIR flower morphogenesis Inferred from mutant phenotype. Source: TAIR leaf morphogenesis Inferred from mutant phenotype. Source: TAIR meristem structural organization Inferred from mutant phenotype. Source: TAIR organ formation Inferred from mutant phenotype. Source: TAIR regulation of meristem growth Inferred from mutant phenotype. Source: TAIR stem cell maintenance Inferred from genetic interaction. Source: TAIR
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro DNA topoisomerase type I activity Inferred from genetic interaction. Source: TAIR protein binding Inferred from physical interaction. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select] Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P30181-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 916

916

DNA topoisomerase 1

PRO_0000145206

Sites

Active site

872

O-(3'-phospho-DNA)-tyrosine intermediate By similarity

Amino acid modifications

Modified residue

122

Phosphoserine Ref.6

Modified residue

170

Phosphoserine Ref.4 Ref.5 Ref.6

Modified residue

286

Phosphothreonine Ref.6

Sequences

Sequence

Length

Mass (Da)

Tools

Isoform 1 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 84BF47913F14454F
Show »

FASTA

916

102,799

        10         20         30         40         50         60 
MGTETVSKPV MDNGSGDSDD DKPLAFKRNN TVASNSNQSK SNSQRSKAVP TTKVSPMRSP 

        70         80         90        100        110        120 
VTSPNGTTPS NKTSIVKSSM PSSSSKASPA KSPLRNDMPS TVKDRSQLQK DQSECKIEHE 

       130        140        150        160        170        180 
DSEDDRPLSS ILSGNKGPTS SRQVSSPQPE KKNNGDRPLD RASRIIKDES DDETPISSMF 

       190        200        210        220        230        240 
RKKIDSGMSG GNQLSNDEKK PLVQKLHQNG STVKNEVPNG KVLGKRPLEK NSSADQSSLK 

       250        260        270        280        290        300 
KAKISASPTS VKMKQDSVKK EIDDKGRVLV SPKMKAKQLS TREDGTDDDD DDDVPISKRF 

       310        320        330        340        350        360 
KSDSSNSNTS SAKPKAVKLN STSSAAKPKA RNVVSPRSRA MTKNTKKVTK DSKYSTSSKS 

       370        380        390        400        410        420 
SPSSGDGQKK WTTLVHNGVI FPPPYKPHGI KILYKGKPVD LTIEQEEVAT MFAVMRETDY 

       430        440        450        460        470        480 
YTKPQFRENF WNDWRRLLGK KHVIQKLDDC DFTPIYEWHL EEKEKKKQMS TEEKKALKEE 

       490        500        510        520        530        540 
KMKQEEKYMW AVVDGVKEKI GNFRVEPPGL FRGRGEHPKM GKLKKRIHPC EITLNIGKGA 

       550        560        570        580        590        600 
PIPECPIAGE RWKEVKHDNT VTWLAFWADP INPKEFKYVF LGAGSSLKGL SDKEKYEKAR 

       610        620        630        640        650        660 
NLTDHIDNIR TTYTKNFTAK DVKMRQIAVA TYLIDKLALR AGNEKDDDEA DTVGCCTLKV 

       670        680        690        700        710        720 
GNVECIPPNK IKFDFLGKDS IQYVNTVEVE PLVYKAIGQF QAGKSKTDDL FDELDTSKLN 

       730        740        750        760        770        780 
AHLKELVPGL TAKVFRTYNA SITLDEMLSQ ETKDGDVTQK IVVYQKANKE VAIICNHQRT 

       790        800        810        820        830        840 
VSKTHGAQIE KLTARIEELK EVLKELKTNL DRAKKGKPPL EGSDGKKIRS LEPNAWEKKI 

       850        860        870        880        890        900 
AQQSAKIEKM ERDMHTKEDL KTVALGTSKI NYLDPRITVA WCKRHEVPIE KIFTKSLLEK 

       910 
FAWAMDVEPE YRFSRR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Kieber J.K., Signer E.R. Submitted (SEP-1990) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence features of the regions of 1,013,767 bp covered by sixteen physically assigned P1 and TAC clones." Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N., Tabata S. DNA Res. 5:297-308(1998) [PubMed: 9872454] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Site-specific phosphorylation profiling of Arabidopsis proteins by mass spectrometry and peptide chip analysis." de la Fuente van Bentem S., Anrather D., Dohnal I., Roitinger E., Csaszar E., Joore J., Buijnink J., Carreri A., Forzani C., Lorkovic Z.J., Barta A., Lecourieux D., Verhounig A., Jonak C., Hirt H. J. Proteome Res. 7:2458-2470(2008) [PubMed: 18433157] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-170, MASS SPECTROMETRY. Tissue: Root.
[5]	"Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana." Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C. J. Proteomics 72:439-451(2009) [PubMed: 19245862] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-170, SUBCELLULAR LOCATION, MASS SPECTROMETRY. Strain: cv. Columbia.
[6]	"Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks." Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S. Plant Physiol. 150:889-903(2009) [PubMed: 19376835] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-122; SER-170 AND THR-286, MASS SPECTROMETRY. Strain: cv. Columbia. Tissue: Seedling.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X57544 mRNA. Translation: CAA40763.1. AB015479 Genomic DNA. Translation: BAB08547.1. CP002688 Genomic DNA. Translation: AED96612.1.
IPI	IPI00541211.
PIR	S22864.
RefSeq	NP_200341.1. NM_124912.4.
UniGene	At.197.
3D structure databases
ProteinModelPortal	P30181.
SMR	P30181. Positions 354-913.
ModBase	Search...
Protein-protein interaction databases
IntAct	P30181. 1 interaction.
STRING	P30181.
Proteomic databases
PRIDE	P30181.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G55300.1; AT5G55300.1; AT5G55300.
GeneID	835623.
GenomeReviews	Gene locus AT5G55300 in contig BA000015_GR.
KEGG	ath:AT5G55300.
NMPDR	fig\|3702.1.peg.27451.
Organism-specific databases
TAIR	At5g55300.
Phylogenomic databases
HOGENOM	HBG521929.
InParanoid	P30181.
OMA	KNFFNDF.
PhylomeDB	P30181.
ProtClustDB	CLSN2686978.
Gene expression databases
Genevestigator	P30181.
GermOnline	AT5G55300. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR011010. DNA_brk_join_enz. IPR013034. DNA_topo_domain1. IPR018521. TopoI_AS. IPR001631. TopoI_C. IPR013499. TopoI_C_euk. IPR014711. TopoI_cat_a-hlx-sub_euk. IPR014727. TopoI_cat_a/b-sub_euk. IPR013500. TopoI_cat_euk. IPR008336. TopoI_DNA-bd_euk. IPR013030. TopoI_DNA-bd_mixed-a/b_euk. [Graphical view]
Gene3D	G3DSA:3.90.15.10. TopoI_cat_a-hlx-sub_euk. 1 hit. G3DSA:1.10.132.10. TopoI_cat_a/b-sub_euk. 1 hit. G3DSA:1.10.10.41. TopoI_DNA-bd_a-hlx_euk. 1 hit. G3DSA:2.170.11.10. TopoI_DNA-bd_mixed-a/b_euk. 2 hits.
KO	K03163.
Pfam	PF01028. Topoisom_I. 1 hit. PF02919. Topoisom_I_N. 1 hit. [Graphical view]
PRINTS	PR00416. EUTPISMRASEI.
SMART	SM00435. TOPEUc. 1 hit. [Graphical view]
SUPFAM	SSF56349. DNA_brk_join_enz. 1 hit. SSF56741. TopoI_DNA_bd_euk. 1 hit.
PROSITE	PS00176. TOPOISOMERASE_I_EUK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOP1_ARATH

Accession

Primary (citable) accession number: P30181

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	December 14, 2011
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents