Reviewed, UniProtKB/Swiss-Prot P30154 (2AAB_HUMAN)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform
Alternative name(s):
    PP2A, subunit A, PR65-beta isoform
    PP2A, subunit A, R1-beta isoform

Gene names

Name:

PPP2R1B

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	601 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit.
Subunit structure	PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with IPO9. Interacts with SGOL1. Ref.9 Ref.10
Domain	Each HEAT repeat appears to consist of two alpha helices joined by a hydrophilic region, the intrarepeat loop. The repeat units may be arranged laterally to form a rod-like structure.
Involvement in disease	Defects in PPP2R1B might be a cause of some lung and colorectal cancers.
Sequence similarities	Belongs to the phosphatase 2A regulatory subunit A family. Contains 15 HEAT repeats.
Sequence caution	The sequence AAA59983.1 differs from that shown. Reason: Miscellaneous discrepancy. Contaminating sequence. Sequence of unknown origin in the N-terminal part.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	antigen binding Inferred from sequence or structural similarity. Source: UniProtKB protein binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PPP2CA	P67775	1	EBI-357094,EBI-712311
PPP2R2A	P63151	1	EBI-357094,EBI-1048931
PPP2R5A	Q15172	1	EBI-357094,EBI-641666
PPP2R5C	Q13362	1	EBI-357094,EBI-1266156
PPP2R5D	Q14738	1	EBI-357094,EBI-396563
PPP2R5E	Q16537	1	EBI-357094,EBI-968374
RALA	P11233	1	EBI-357094,EBI-1036803

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P30154-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P30154-2)

The sequence of this isoform differs from the canonical sequence as follows:
     598-601: LALA → VAQRLRKLEFPVKDSGEPSVPRADKNHFPRPTVPGEDMGKGPVYQLRGDTRDTLAQLGIAELVHFSQSTD

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 601	601	Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform		PRO_0000071403
Regions
	Repeat	20 – 58	39	HEAT 1
	Repeat	59 – 96	38	HEAT 2
	Repeat	97 – 135	39	HEAT 3
	Repeat	136 – 173	38	HEAT 4
	Repeat	174 – 212	39	HEAT 5
	Repeat	213 – 251	39	HEAT 6
	Repeat	252 – 290	39	HEAT 7
	Repeat	291 – 333	43	HEAT 8
	Repeat	334 – 372	39	HEAT 9
	Repeat	373 – 411	39	HEAT 10
	Repeat	412 – 450	39	HEAT 11
	Repeat	451 – 489	39	HEAT 12
	Repeat	490 – 528	39	HEAT 13
	Repeat	529 – 567	39	HEAT 14
	Repeat	568 – 601	34	HEAT 15
Natural variations
	Alternative sequence	598 – 601	4	LALA → VAQRLRKLEFPVKDSGEPSV PRADKNHFPRPTVPGEDMGK GPVYQLRGDTRDTLAQLGIA ELVHFSQSTD in isoform 2.		VSP_036460
	Natural variant	8	1	G → R in a lung cancer patient. Ref.2		VAR_022895
	Natural variant	15	1	G → A in a colorectal cancer patient. Ref.13		VAR_022896
	Natural variant	65	1	P → S in a lung cancer patient. Ref.2		VAR_022897
	Natural variant	90	1	G → D in a lung cancer patient. dbSNP rs1805076. Ref.2 Ref.12 Ref.14		VAR_006384
	Natural variant	101	1	L → P in a colon adenocarcinoma. Ref.2		VAR_022898
	Natural variant	343	1	K → E in a lung cancer patient. Ref.2		VAR_022899
	Natural variant	365	1	S → P in a colorectal cancer patient. Ref.13		VAR_022900
	Natural variant	448	1	V → A in a colon adenocarcinoma. Ref.2		VAR_022901
	Natural variant	498	1	V → E in a colorectal cancer patient. Ref.13		VAR_022902
	Natural variant	499	1	L → I in a colorectal cancer patient. Ref.13		VAR_022903
	Natural variant	500	1	V → G in a colorectal cancer patient. Ref.13		VAR_022904
	Natural variant	504	1	D → G in a lung cancer patient. Ref.2		VAR_022905
	Natural variant	545	1	V → A in a colon adenocarcinoma. Ref.2		VAR_022906
Experimental info
	Sequence conflict	74	1	E → V in AAA59983. Ref.8
	Sequence conflict	178	1	I → T in AAC63525. Ref.1
	Sequence conflict	178	1	I → T in AAG39644. Ref.3
	Sequence conflict	178	1	I → T in AAA59983. Ref.8
	Sequence conflict	411	1	Q → R in AAA59983. Ref.8

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 17, 2006. Version 3. Checksum: 86AB20D7505210B0	FASTA	601	66,214
	10 20 30 40 50 60 MAGASELGTG PGAAGGDGDD SLYPIAVLID ELRNEDVQLR LNSIKKLSTI ALALGVERTR 70 80 90 100 110 120 SELLPFLTDT IYDEDEVLLA LAEQLGNFTG LVGGPDFAHC LLPPLENLAT VEETVVRDKA 130 140 150 160 170 180 VESLRQISQE HTPVALEAYF VPLVKRLASG DWFTSRTSAC GLFSVCYPRA SNAVKAEIRQ 190 200 210 220 230 240 QFRSLCSDDT PMVRRAAASK LGEFAKVLEL DSVKSEIVPL FTSLASDEQD SVRLLAVEAC 250 260 270 280 290 300 VSIAQLLSQD DLETLVMPTL RQAAEDKSWR VRYMVADRFS ELQKAMGPKI TLNDLIPAFQ 310 320 330 340 350 360 NLLKDCEAEV RAAAAHKVKE LGENLPIEDR ETIIMNQILP YIKELVSDTN QHVKSALASV 370 380 390 400 410 420 IMGLSTILGK ENTIEHLLPL FLAQLKDECP DVRLNIISNL DCVNEVIGIR QLSQSLLPAI 430 440 450 460 470 480 VELAEDAKWR VRLAIIEYMP LLAGQLGVEF FDEKLNSLCM AWLVDHVYAI REAATNNLMK 490 500 510 520 530 540 LVQKFGTEWA QNTIVPKVLV MANDPNYLHR MTTLFCINAL SEACGQEITT KQMLPIVLKM 550 560 570 580 590 600 AGDQVANVRF NVAKSLQKIG PILDTNALQG EVKPVLQKLG QDEDMDVKYF AQEAISVLAL A
	Isoform 2. Checksum: 726EA8A8FB0DEB23	FASTA	667	73,585
	10 20 30 40 50 60 MAGASELGTG PGAAGGDGDD SLYPIAVLID ELRNEDVQLR LNSIKKLSTI ALALGVERTR 70 80 90 100 110 120 SELLPFLTDT IYDEDEVLLA LAEQLGNFTG LVGGPDFAHC LLPPLENLAT VEETVVRDKA 130 140 150 160 170 180 VESLRQISQE HTPVALEAYF VPLVKRLASG DWFTSRTSAC GLFSVCYPRA SNAVKAEIRQ 190 200 210 220 230 240 QFRSLCSDDT PMVRRAAASK LGEFAKVLEL DSVKSEIVPL FTSLASDEQD SVRLLAVEAC 250 260 270 280 290 300 VSIAQLLSQD DLETLVMPTL RQAAEDKSWR VRYMVADRFS ELQKAMGPKI TLNDLIPAFQ 310 320 330 340 350 360 NLLKDCEAEV RAAAAHKVKE LGENLPIEDR ETIIMNQILP YIKELVSDTN QHVKSALASV 370 380 390 400 410 420 IMGLSTILGK ENTIEHLLPL FLAQLKDECP DVRLNIISNL DCVNEVIGIR QLSQSLLPAI 430 440 450 460 470 480 VELAEDAKWR VRLAIIEYMP LLAGQLGVEF FDEKLNSLCM AWLVDHVYAI REAATNNLMK 490 500 510 520 530 540 LVQKFGTEWA QNTIVPKVLV MANDPNYLHR MTTLFCINAL SEACGQEITT KQMLPIVLKM 550 560 570 580 590 600 AGDQVANVRF NVAKSLQKIG PILDTNALQG EVKPVLQKLG QDEDMDVKYF AQEAISVVAQ 610 620 630 640 650 660 RLRKLEFPVK DSGEPSVPRA DKNHFPRPTV PGEDMGKGPV YQLRGDTRDT LAQLGIAELV HFSQSTD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genomic organization and precise physical location of protein phosphatase 2A regulatory subunit A beta isoform gene on chromosome band 11q23." Baysal B.E., Farr J.E., Goss J.R., Devlin B., Richard C.W. III Gene 217:107-116(1998) [PubMed: 9795170] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Alterations of the PPP2R1B gene in human lung and colon cancer." Wang S.S., Esplin E.D., Li J.L., Huang L., Gazdar A., Minna J., Evans G.A. Science 282:284-287(1998) [PubMed: 9765152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS ARG-8; SER-65; ASP-90; PRO-101; GLU-343; ALA-448; GLY-504 AND ALA-545.
[3]	"A high-resolution integrated map spanning the SDHD gene at 11q23: a 1.1-Mb BAC contig, a partial transcript map and 15 new repeat polymorphisms in a tumour-suppressor region." Baysal B.E., Willett-Brozick J.E., Taschner P.E.M., Dauwerse J.G., Devilee P., Devlin B. Eur. J. Hum. Genet. 9:121-129(2001) [PubMed: 11313745] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]	NHLBI resequencing and genotyping service (RS&G) Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed: 16554811] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Testis.
[8]	"Alpha- and beta-forms of the 65-kDa subunit of protein phosphatase 2A have a similar 39 amino acid repeating structure." Hemmings B.A., Adams-Pearson C., Maurer F., Mueller P., Goris J., Merlevede W., Hofsteenge J., Stone S.R. Biochemistry 29:3166-3173(1990) [PubMed: 2159327] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 31-601 (ISOFORM 1).
[9]	"Interaction between protein phosphatase 2A and members of the importin beta superfamily." Lubert E.J., Sarge K.D. Biochem. Biophys. Res. Commun. 303:908-913(2003) [PubMed: 12670497] [Abstract] Cited for: INTERACTION WITH IPO9.
[10]	"Shugoshin collaborates with protein phosphatase 2A to protect cohesin." Kitajima T.S., Sakuno T., Ishiguro K., Iemura S., Natsume T., Kawashima S.A., Watanabe Y. Nature 441:46-52(2006) [PubMed: 16541025] [Abstract] Cited for: INTERACTION WITH SGOL1.
[11]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[12]	"Absence of PPP2R1B gene alterations in primary ovarian cancers." Campbell I.G., Manolitsas T. Oncogene 18:6367-6369(1999) [PubMed: 10597236] [Abstract] Cited for: VARIANT ASP-90.
[13]	"Alterations of the PPP2R1B gene located at 11q23 in human colorectal cancers." Takagi Y., Futamura M., Yamaguchi K., Aoki S., Takahashi T., Saji S. Gut 47:268-271(2000) [PubMed: 10896920] [Abstract] Cited for: VARIANTS ALA-15; PRO-365; GLU-498; ILE-499 AND GLY-500.
[14]	"Alterations in the suppressor gene PPP2R1B in parathyroid hyperplasias and adenomas." Hemmer S., Wasenius V.M., Haglund C., Zhu Y., Knuutila S., Franssila K., Joensuu H. Cancer Genet. Cytogenet. 134:13-17(2002) [PubMed: 11996789] [Abstract] Cited for: VARIANT ASP-90.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF083439 , AF083425, AF083426, AF083427, AF083428, AF083429, AF083430, AF083431, AF083432, AF083433, AF083434, AF083435, AF083436, AF083437, AF083438 Genomic DNA. Translation: AAC63525.1. AF087438 mRNA. Translation: AAC69624.1. AF163473 mRNA. Translation: AAG39644.1. EF445011 Genomic DNA. Translation: ACA06047.1. AP000925 Genomic DNA. No translation available. AP001781 Genomic DNA. No translation available. CH471065 Genomic DNA. Translation: EAW67151.1. BC027596 mRNA. Translation: AAH27596.1. M65254 mRNA. Translation: AAA59983.1. Sequence problems.
IPI	IPI00294178. IPI00335449.
PIR	B34541.
RefSeq	NP_002707.3. NP_859050.1.
UniGene	Hs.584790
3D structure databases
HSSP	HSSP built from PDB template 1B3U based on UniProtKB P30153.
SMR	P30154. Positions 14-601.
ModBase	Search...
Protein-protein interaction databases
IntAct	P30154. 31 interactions.
STRING	P30154.
Proteomic databases
PRIDE	P30154.
Genome annotation databases
Ensembl	ENST00000311129; ENSP00000311344; ENSG00000137713; Homo sapiens. [Genome view] ENST00000341980; ENSP00000343317; ENSG00000137713; Homo sapiens. [Genome view] ENST00000393055; ENSP00000376775; ENSG00000137713; Homo sapiens. [Genome view] ENST00000412902; ENSP00000403301; ENSG00000137713; Homo sapiens. [Genome view] ENST00000426998; ENSP00000410671; ENSG00000137713; Homo sapiens. [Genome view] ENST00000427203; ENSP00000415759; ENSG00000137713; Homo sapiens. [Genome view]
GeneID	5519.
KEGG	hsa:5519.
UCSC	uc001plw.1. human. uc001plx.1. human.
Organism-specific databases
CTD	5519.
GeneCards	GC11M111102.
HGNC	HGNC:9303. PPP2R1B.
HPA	CAB004034.
MIM	603113. gene.
PharmGKB	PA33667.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	P30154.
OMA	HVNTRDT.
Enzyme and pathway databases
Reactome	REACT_11045. Signaling by Wnt. REACT_11061. Signalling by NGF. REACT_1505. Integration of energy metabolism. REACT_152. Cell Cycle, Mitotic. REACT_15295. Opioid Signalling. REACT_6900. Signaling in Immune system.
Gene expression databases
ArrayExpress	P30154.
Bgee	P30154.
CleanEx	HS_PPP2R1B.
Genevestigator	P30154.
GermOnline	ENSG00000137713. Homo sapiens.
Family and domain databases
InterPro	IPR011989. ARM-like. IPR000357. HEAT. [Graphical view]
Gene3D	G3DSA:1.25.10.10. ARM-like. 1 hit.
Pfam	PF02985. HEAT. 11 hits. [Graphical view]
PROSITE	PS50077. HEAT_REPEAT. 12 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	21346.
SOURCE	Search...

Entry information

Entry name

2AAB_HUMAN

Accession

Primary (citable) accession number: P30154
Secondary accession number(s): O75620, Q8NHV8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	October 17, 2006
Last modified:	November 3, 2009
This is version 92 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.