Hydroxypyruvate isomerase - Escherichia coli (strain K12)

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P30147 (HYI_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 94. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Hydroxypyruvate isomerase
EC=5.3.1.22

Alternative name(s):
Glyoxylate-induced protein

Gene names

Name:	hyi
Synonyms:	gip, ybbG
Ordered Locus Names:	b0508, JW0496

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the reversible isomerization between hydroxypyruvate and 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde). Does not catalyze the isomerization of D-fructose to D-glucose or that of D-xylulose to D-xylose. Also does not catalyze racemization of serine, alanine, glycerate or lactate. Ref.2
Catalytic activity	Hydroxypyruvate = 2-hydroxy-3-oxopropanoate. Ref.2
Enzyme regulation	Not stimulated by addition of pyridoxal 5'-phosphate (0.1 mM), FAD, NAD⁺, NADP⁺ or ATP (1 mM each). EDTA (10 mM) and metal ions (1 mM) such as Ca²⁺, Co²⁺, Mg²⁺, Ni²⁺, Zn²⁺ do not affect the enzyme activity. Ref.2
Subunit structure	Homodimer. Ref.2
Induction	By glyoxylate. Ref.2
Disruption phenotype	Does not affect growth on glycolate or glyoxylate. Ref.1
Sequence similarities	Belongs to the hyi family.
Biophysicochemical properties	Kinetic parameters: K_M=12.5 mM for hydroxypyruvate (at 37 degrees Celsius and pH 7.0) Ref.2 V_max=14.3 µmol/min/mg enzyme (at 37 degrees Celsius and pH 7.0) pH dependence: Optimum pH is 6.8-7.2.

Ontologies

Keywords
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glyoxylate metabolic process Inferred from direct assay Ref.2. Source: FlyBase
Molecular_function	hydroxypyruvate isomerase activity Inferred from direct assay Ref.2. Source: FlyBase protein homodimerization activity Inferred from direct assay Ref.2. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 258

258

Hydroxypyruvate isomerase

PRO_0000209106

Sequences

Sequence

Length

Mass (Da)

Tools

P30147 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 3F001A47FADE5918
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FASTA

258

29,377

        10         20         30         40         50         60 
MLRFSANLSM LFGEYDFLAR FEKAAQCGFR GVEFMFPYDY DIEELKHVLA SNKLEHTLHN 

        70         80         90        100        110        120 
LPAGDWAAGE RGIACIPGRE EEFRDGVAAA IRYARALGNK KINCLVGKTP AGFSSEQIHA 

       130        140        150        160        170        180 
TLVENLRYAA NMLMKEDILL LIEPINHFDI PGFHLTGTRQ ALKLIDDVGC CNLKIQYDIY 

       190        200        210        220        230        240 
HMQRMEGELT NTMTQWADKI GHLQIADNPH RGEPGTGEIN YDYLFKVIEN SDYNGWVGCE 

       250 
YKPQTTTEAG LRWMDPYR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, DNA sequencing, and biochemical analyses of Escherichia coli glyoxylate carboligase. An enzyme of the acetohydroxy acid synthase-pyruvate oxidase family." Chang Y.-Y., Wang A.-Y., Cronan J.E. Jr. J. Biol. Chem. 268:3911-3919(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE. Strain: K12.
[2]	"Biochemical evidence that Escherichia coli hyi (orf b0508, gip) gene encodes hydroxypyruvate isomerase." Ashiuchi M., Misono H. Biochim. Biophys. Acta 1435:153-159(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT. Strain: K12 / JM109 / ATCC 53323.
[3]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L03845 Genomic DNA. Translation: AAA23865.1. AB028043 Genomic DNA. Translation: BAA89011.1. U82664 Genomic DNA. Translation: AAB40261.1. U00096 Genomic DNA. Translation: AAC73610.1. AP009048 Genomic DNA. Translation: BAE76286.1.
PIR	JT0746.
RefSeq	NP_415041.1. NC_000913.2. YP_488798.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P30147.
SMR	P30147. Positions 1-258.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-9994N.
IntAct	P30147. 7 interactions.
STRING	511145.b0508.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73610; AAC73610; b0508. BAE76286; BAE76286; BAE76286.
GeneID	12930869. 946186.
KEGG	ecj:Y75_p0494. eco:b0508.
PATRIC	32116175. VBIEscCol129921_0529.
Organism-specific databases
EchoBASE	EB1543.
EcoGene	EG11584. hyi.
Phylogenomic databases
eggNOG	COG3622.
HOGENOM	HOG000218197.
KO	K01816.
OMA	EPINKYA.
ProtClustDB	PRK09997.
Enzyme and pathway databases
BioCyc	EcoCyc:G6277-MONOMER. ECOL316407:JW0496-MONOMER. MetaCyc:G6277-MONOMER.
Gene expression databases
Genevestigator	P30147.
Family and domain databases
Gene3D	3.20.20.150. 1 hit.
InterPro	IPR017643. Hydroxypyruvate_isomerase. IPR026040. HyI-like. IPR013022. Xyl_isomerase-like_TIM-brl. [Graphical view]
Pfam	PF01261. AP_endonuc_2. 1 hit. [Graphical view]
PIRSF	PIRSF006241. HyI. 1 hit.
SUPFAM	SSF51658. Xyl_isomerase-like_TIM-brl. 1 hit.
TIGRFAMs	TIGR03234. OH-pyruv-isom. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HYI_ECOLI

Accession

Primary (citable) accession number: P30147
Secondary accession number(s): Q2MBS0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents