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Protein

Hydroxypyruvate isomerase

Gene

hyi

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible isomerization between hydroxypyruvate and 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde). Does not catalyze the isomerization of D-fructose to D-glucose or that of D-xylulose to D-xylose. Also does not catalyze racemization of serine, alanine, glycerate or lactate.1 Publication

Catalytic activityi

Hydroxypyruvate = 2-hydroxy-3-oxopropanoate.1 Publication

Enzyme regulationi

Not stimulated by addition of pyridoxal 5'-phosphate (0.1 mM), FAD, NAD+, NADP+ or ATP (1 mM each). EDTA (10 mM) and metal ions (1 mM) such as Ca2+, Co2+, Mg2+, Ni2+, Zn2+ do not affect the enzyme activity.1 Publication

Kineticsi

  1. KM=12.5 mM for hydroxypyruvate (at 37 degrees Celsius and pH 7.0)1 Publication
  1. Vmax=14.3 µmol/min/mg enzyme (at 37 degrees Celsius and pH 7.0)1 Publication

pH dependencei

Optimum pH is 6.8-7.2.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei143Proton donor/acceptorBy similarity1
Active sitei240Proton donor/acceptorBy similarity1

GO - Molecular functioni

  • hydroxypyruvate isomerase activity Source: FlyBase
  • protein homodimerization activity Source: FlyBase

GO - Biological processi

  • glyoxylate metabolic process Source: FlyBase

Keywordsi

Molecular functionIsomerase

Enzyme and pathway databases

BioCyciEcoCyc:G6277-MONOMER
MetaCyc:G6277-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxypyruvate isomerase (EC:5.3.1.22)
Alternative name(s):
Glyoxylate-induced protein
Gene namesi
Name:hyi
Synonyms:gip, ybbG
Ordered Locus Names:b0508, JW0496
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11584 hyi

Pathology & Biotechi

Disruption phenotypei

Does not affect growth on glycolate or glyoxylate.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002091061 – 258Hydroxypyruvate isomeraseAdd BLAST258

Proteomic databases

PaxDbiP30147
PRIDEiP30147

Expressioni

Inductioni

By glyoxylate.

Interactioni

Subunit structurei

Homodimer.1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: FlyBase

Protein-protein interaction databases

BioGridi4259863, 8 interactors
DIPiDIP-9994N
IntActiP30147, 12 interactors
STRINGi316385.ECDH10B_0464

Structurei

3D structure databases

ProteinModelPortaliP30147
SMRiP30147
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the hyi family.Curated

Phylogenomic databases

eggNOGiENOG4105QG4 Bacteria
COG3622 LUCA
HOGENOMiHOG000218197
InParanoidiP30147
KOiK01816
OMAiYVQYDIY
PhylomeDBiP30147

Family and domain databases

InterProiView protein in InterPro
IPR017643 Hydroxypyruvate_isomerase
IPR026040 HyI-like
IPR036237 Xyl_isomerase-like_sf
IPR013022 Xyl_isomerase-like_TIM-brl
PfamiView protein in Pfam
PF01261 AP_endonuc_2, 1 hit
PIRSFiPIRSF006241 HyI, 1 hit
SUPFAMiSSF51658 SSF51658, 1 hit
TIGRFAMsiTIGR03234 OH-pyruv-isom, 1 hit

Sequencei

Sequence statusi: Complete.

P30147-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRFSANLSM LFGEYDFLAR FEKAAQCGFR GVEFMFPYDY DIEELKHVLA
60 70 80 90 100
SNKLEHTLHN LPAGDWAAGE RGIACIPGRE EEFRDGVAAA IRYARALGNK
110 120 130 140 150
KINCLVGKTP AGFSSEQIHA TLVENLRYAA NMLMKEDILL LIEPINHFDI
160 170 180 190 200
PGFHLTGTRQ ALKLIDDVGC CNLKIQYDIY HMQRMEGELT NTMTQWADKI
210 220 230 240 250
GHLQIADNPH RGEPGTGEIN YDYLFKVIEN SDYNGWVGCE YKPQTTTEAG

LRWMDPYR
Length:258
Mass (Da):29,377
Last modified:April 1, 1993 - v1
Checksum:i3F001A47FADE5918
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L03845 Genomic DNA Translation: AAA23865.1
AB028043 Genomic DNA Translation: BAA89011.1
U82664 Genomic DNA Translation: AAB40261.1
U00096 Genomic DNA Translation: AAC73610.1
AP009048 Genomic DNA Translation: BAE76286.1
PIRiJT0746
RefSeqiNP_415041.1, NC_000913.3
WP_000943544.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73610; AAC73610; b0508
BAE76286; BAE76286; BAE76286
GeneIDi946186
KEGGiecj:JW0496
eco:b0508
PATRICifig|1411691.4.peg.1769

Similar proteinsi

Entry informationi

Entry nameiHYI_ECOLI
AccessioniPrimary (citable) accession number: P30147
Secondary accession number(s): Q2MBS0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: March 28, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health