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Protein

Hydroxypyruvate isomerase

Gene

hyi

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible isomerization between hydroxypyruvate and 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde). Does not catalyze the isomerization of D-fructose to D-glucose or that of D-xylulose to D-xylose. Also does not catalyze racemization of serine, alanine, glycerate or lactate.1 Publication

Catalytic activityi

Hydroxypyruvate = 2-hydroxy-3-oxopropanoate.1 Publication

Enzyme regulationi

Not stimulated by addition of pyridoxal 5'-phosphate (0.1 mM), FAD, NAD+, NADP+ or ATP (1 mM each). EDTA (10 mM) and metal ions (1 mM) such as Ca2+, Co2+, Mg2+, Ni2+, Zn2+ do not affect the enzyme activity.1 Publication

Kineticsi

  1. KM=12.5 mM for hydroxypyruvate (at 37 degrees Celsius and pH 7.0)1 Publication
  1. Vmax=14.3 µmol/min/mg enzyme (at 37 degrees Celsius and pH 7.0)1 Publication

pH dependencei

Optimum pH is 6.8-7.2.1 Publication

GO - Molecular functioni

  • hydroxypyruvate isomerase activity Source: FlyBase
  • protein homodimerization activity Source: FlyBase

GO - Biological processi

  • glyoxylate metabolic process Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

BioCyciEcoCyc:G6277-MONOMER.
ECOL316407:JW0496-MONOMER.
MetaCyc:G6277-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxypyruvate isomerase (EC:5.3.1.22)
Alternative name(s):
Glyoxylate-induced protein
Gene namesi
Name:hyi
Synonyms:gip, ybbG
Ordered Locus Names:b0508, JW0496
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11584. hyi.

Pathology & Biotechi

Disruption phenotypei

Does not affect growth on glycolate or glyoxylate.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 258258Hydroxypyruvate isomerasePRO_0000209106Add
BLAST

Proteomic databases

PaxDbiP30147.

Expressioni

Inductioni

By glyoxylate.

Interactioni

Subunit structurei

Homodimer.1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: FlyBase

Protein-protein interaction databases

BioGridi4259863. 8 interactions.
DIPiDIP-9994N.
IntActiP30147. 12 interactions.
STRINGi511145.b0508.

Structurei

3D structure databases

ProteinModelPortaliP30147.
SMRiP30147. Positions 1-258.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the hyi family.Curated

Phylogenomic databases

eggNOGiENOG4105QG4. Bacteria.
COG3622. LUCA.
HOGENOMiHOG000218197.
InParanoidiP30147.
KOiK01816.
OMAiPDRVSEF.
OrthoDBiEOG6BS8PW.
PhylomeDBiP30147.

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
InterProiIPR017643. Hydroxypyruvate_isomerase.
IPR026040. HyI-like.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PfamiPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006241. HyI. 1 hit.
SUPFAMiSSF51658. SSF51658. 1 hit.
TIGRFAMsiTIGR03234. OH-pyruv-isom. 1 hit.

Sequencei

Sequence statusi: Complete.

P30147-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRFSANLSM LFGEYDFLAR FEKAAQCGFR GVEFMFPYDY DIEELKHVLA
60 70 80 90 100
SNKLEHTLHN LPAGDWAAGE RGIACIPGRE EEFRDGVAAA IRYARALGNK
110 120 130 140 150
KINCLVGKTP AGFSSEQIHA TLVENLRYAA NMLMKEDILL LIEPINHFDI
160 170 180 190 200
PGFHLTGTRQ ALKLIDDVGC CNLKIQYDIY HMQRMEGELT NTMTQWADKI
210 220 230 240 250
GHLQIADNPH RGEPGTGEIN YDYLFKVIEN SDYNGWVGCE YKPQTTTEAG

LRWMDPYR
Length:258
Mass (Da):29,377
Last modified:April 1, 1993 - v1
Checksum:i3F001A47FADE5918
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L03845 Genomic DNA. Translation: AAA23865.1.
AB028043 Genomic DNA. Translation: BAA89011.1.
U82664 Genomic DNA. Translation: AAB40261.1.
U00096 Genomic DNA. Translation: AAC73610.1.
AP009048 Genomic DNA. Translation: BAE76286.1.
PIRiJT0746.
RefSeqiNP_415041.1. NC_000913.3.
WP_000943544.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73610; AAC73610; b0508.
BAE76286; BAE76286; BAE76286.
GeneIDi946186.
KEGGiecj:JW0496.
eco:b0508.
PATRICi32116175. VBIEscCol129921_0529.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L03845 Genomic DNA. Translation: AAA23865.1.
AB028043 Genomic DNA. Translation: BAA89011.1.
U82664 Genomic DNA. Translation: AAB40261.1.
U00096 Genomic DNA. Translation: AAC73610.1.
AP009048 Genomic DNA. Translation: BAE76286.1.
PIRiJT0746.
RefSeqiNP_415041.1. NC_000913.3.
WP_000943544.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP30147.
SMRiP30147. Positions 1-258.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259863. 8 interactions.
DIPiDIP-9994N.
IntActiP30147. 12 interactions.
STRINGi511145.b0508.

Proteomic databases

PaxDbiP30147.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73610; AAC73610; b0508.
BAE76286; BAE76286; BAE76286.
GeneIDi946186.
KEGGiecj:JW0496.
eco:b0508.
PATRICi32116175. VBIEscCol129921_0529.

Organism-specific databases

EchoBASEiEB1543.
EcoGeneiEG11584. hyi.

Phylogenomic databases

eggNOGiENOG4105QG4. Bacteria.
COG3622. LUCA.
HOGENOMiHOG000218197.
InParanoidiP30147.
KOiK01816.
OMAiPDRVSEF.
OrthoDBiEOG6BS8PW.
PhylomeDBiP30147.

Enzyme and pathway databases

BioCyciEcoCyc:G6277-MONOMER.
ECOL316407:JW0496-MONOMER.
MetaCyc:G6277-MONOMER.

Miscellaneous databases

PROiP30147.

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
InterProiIPR017643. Hydroxypyruvate_isomerase.
IPR026040. HyI-like.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PfamiPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006241. HyI. 1 hit.
SUPFAMiSSF51658. SSF51658. 1 hit.
TIGRFAMsiTIGR03234. OH-pyruv-isom. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning, DNA sequencing, and biochemical analyses of Escherichia coli glyoxylate carboligase. An enzyme of the acetohydroxy acid synthase-pyruvate oxidase family."
    Chang Y.-Y., Wang A.-Y., Cronan J.E. Jr.
    J. Biol. Chem. 268:3911-3919(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE.
    Strain: K12.
  2. "Biochemical evidence that Escherichia coli hyi (orf b0508, gip) gene encodes hydroxypyruvate isomerase."
    Ashiuchi M., Misono H.
    Biochim. Biophys. Acta 1435:153-159(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
    Strain: K12 / JM109 / ATCC 53323.
  3. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiHYI_ECOLI
AccessioniPrimary (citable) accession number: P30147
Secondary accession number(s): Q2MBS0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: January 20, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.