P30143 (YAAJ_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized transporter YaaJ

Gene names

Name:	yaaJ
Ordered Locus Names:	b0007, JW0006

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Subcellular location	Cell inner membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the sodium:alanine (SAF) symporter family.

Ontologies

Keywords
Biological process	Symport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	alanine:sodium symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 476	476	Uncharacterized transporter YaaJ		PRO_0000161566
Regions
	Transmembrane	4 – 24	21	Helical; Potential
	Transmembrane	81 – 101	21	Helical; Potential
	Transmembrane	141 – 161	21	Helical; Potential
	Transmembrane	174 – 194	21	Helical; Potential
	Transmembrane	207 – 227	21	Helical; Potential
	Transmembrane	233 – 253	21	Helical; Potential
	Transmembrane	300 – 320	21	Helical; Potential
	Transmembrane	351 – 371	21	Helical; Potential
	Transmembrane	391 – 411	21	Helical; Potential
	Transmembrane	414 – 434	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P30143 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 2F6EB2E12E126E63	FASTA	476	51,663
	10 20 30 40 50 60 MPDFFSFINS VLWGSVMIYL LFGAGCWFTF RTGFVQFRYI RQFGKSLKNS IHPQPGGLTS 70 80 90 100 110 120 FQSLCTSLAA RVGSGNLAGV ALAITAGGPG AVFWMWVAAF IGMATSFAEC SLAQLYKERD 130 140 150 160 170 180 VNGQFRGGPA WYMARGLGMR WMGVLFAVFL LIAYGIIFSG VQANAVARAL SFSFDFPPLV 190 200 210 220 230 240 TGIILAVFTL LAITRGLHGV ARLMQGFVPL MAIIWVLTSL VICVMNIGQL PHVIWSIFES 250 260 270 280 290 300 AFGWQEAAGG AAGYTLSQAI TNGFQRSMFS NEAGMGSTPN AAAAAASWPP HPAAQGIVQM 310 320 330 340 350 360 IGIFIDTLVI CTASAMLILL AGNGTTYMPL EGIQLIQKAM RVLMGSWGAE FVTLVVILFA 370 380 390 400 410 420 FSSIVANYIY AENNLFFLRL NNPKAIWCLR ICTFATVIGG TLLSLPLMWQ LADIIMACMA 430 440 450 460 470 ITNLTAILLL SPVVHTIASD YLRQRKLGVR PVFDPLRYPD IGRQLSPDAW DDVSQE

References

[1]	"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC73118.1. AP009048 Genomic DNA. Translation: BAB96585.1.
PIR	G64720.
RefSeq	NP_414548.1. NC_000913.2. YP_488313.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P30143.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b0007.
Proteomic databases
PRIDE	P30143.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73118; AAC73118; b0007. BAB96585; BAB96585; BAB96585.
GeneID	12932944. 944745.
KEGG	ecj:Y75_p0007. eco:b0007.
PATRIC	32115109. VBIEscCol129921_0006.
Organism-specific databases
EchoBASE	EB1516.
EcoGene	EG11555. yaaJ.
Phylogenomic databases
eggNOG	COG1115.
HOGENOM	HOG000255107.
KO	K03310.
OMA	TLVICTS.
ProtClustDB	CLSK879538.
Enzyme and pathway databases
BioCyc	EcoCyc:YAAJ-MONOMER. ECOL316407:JW0006-MONOMER.
Gene expression databases
Genevestigator	P30143.
Family and domain databases
InterPro	IPR001463. Na/Ala_symport. [Graphical view]
Pfam	PF01235. Na_Ala_symp. 1 hit. [Graphical view]
PRINTS	PR00175. NAALASMPORT.
TIGRFAMs	TIGR00835. agcS. 1 hit.
PROSITE	PS00873. NA_ALANINE_SYMP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YAAJ_ECOLI

Accession

Primary (citable) accession number: P30143

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	May 1, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program