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Protein

2-iminoacetate synthase

Gene

thiH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol.2 Publications

Miscellaneous

The product 2-iminoacetate hydrates in vitro to yield glyoxylate and ammonium.

Catalytic activityi

L-tyrosine + S-adenosyl-L-methionine + NADPH = 2-iminoacetate + 4-methylphenol + 5'-deoxyadenosine + L-methionine + NADP+ + H+.2 Publications

Cofactori

[4Fe-4S] cluster2 PublicationsNote: Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.2 Publications

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi85Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi89Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi92Iron-sulfur (4Fe-4S-S-AdoMet)By similarity1

GO - Molecular functioni

  • 2-iminoacetate synthase activity Source: EcoCyc
  • 4 iron, 4 sulfur cluster binding Source: EcoCyc
  • iron ion binding Source: InterPro

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • thiamine biosynthetic process Source: EcoCyc
  • thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionLyase
Biological processThiamine biosynthesis
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, NADP, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:THIH-MONOMER
MetaCyc:THIH-MONOMER
BRENDAi4.1.99.19 2026
UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
2-iminoacetate synthase (EC:4.1.99.192 Publications)
Alternative name(s):
Dehydroglycine synthase
Tyrosine lyase
Gene namesi
Name:thiH
Ordered Locus Names:b3990, JW3953
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi

Organism-specific databases

EcoGeneiEG11590 thiH

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000725141 – 3772-iminoacetate synthaseAdd BLAST377

Proteomic databases

PaxDbiP30140
PRIDEiP30140

Interactioni

Subunit structurei

Forms a heterodimer with ThiG.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
thiGP301392EBI-1125553,EBI-547059

Protein-protein interaction databases

BioGridi4262909, 13 interactors
DIPiDIP-6869N
IntActiP30140, 4 interactors
STRINGi316385.ECDH10B_4178

Structurei

3D structure databases

ProteinModelPortaliP30140
SMRiP30140
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. ThiH family.Curated

Phylogenomic databases

eggNOGiENOG4105D41 Bacteria
COG1060 LUCA
HOGENOMiHOG000270732
InParanoidiP30140
KOiK03150
OMAiLICAYRL
PhylomeDBiP30140

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR010722 BATS_dom
IPR034416 BATS_domain_containing
IPR007197 rSAM
IPR012726 ThiH
PANTHERiPTHR43583:SF1 PTHR43583:SF1, 1 hit
PfamiView protein in Pfam
PF06968 BATS, 1 hit
PF04055 Radical_SAM, 1 hit
SFLDiSFLDF00301 2-iminoacetate_synthase_(ThiH), 1 hit
SFLDG01060 BATS_domain_containing, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00876 BATS, 1 hit
TIGRFAMsiTIGR02351 thiH, 1 hit

Sequencei

Sequence statusi: Complete.

P30140-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTFSDRWRQ LDWDDIRLRI NGKTAADVER ALNASQLTRD DMMALLSPAA
60 70 80 90 100
SGYLEQLAQR AQRLTRQRFG NTVSFYVPLY LSNLCANDCT YCGFSMSNRI
110 120 130 140 150
KRKTLDEADI ARESAAIREM GFEHLLLVTG EHQAKVGMDY FRRHLPALRE
160 170 180 190 200
QFSSLQMEVQ PLAETEYAEL KQLGLDGVMV YQETYHEATY ARHHLKGKKQ
210 220 230 240 250
DFFWRLETPD RLGRAGIDKI GLGALIGLSD NWRVDSYMVA EHLLWLQQHY
260 270 280 290 300
WQSRYSVSFP RLRPCTGGIE PASIMDERQL VQTICAFRLL APEIELSLST
310 320 330 340 350
RESPWFRDRV IPLAINNVSA FSKTQPGGYA DNHPELEQFS PHDDRRPEAV
360 370
AAALTAQGLQ PVWKDWDSYL GRASQRL
Length:377
Mass (Da):43,320
Last modified:January 23, 2007 - v3
Checksum:iF45A0859372F5ED6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88701 Genomic DNA Translation: AAB95619.1
U00006 Genomic DNA Translation: AAC43088.1
U00096 Genomic DNA Translation: AAC76964.1
AP009048 Genomic DNA Translation: BAE77330.1
PIRiS35121
RefSeqiNP_418417.1, NC_000913.3
WP_000847447.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76964; AAC76964; b3990
BAE77330; BAE77330; BAE77330
GeneIDi948494
KEGGiecj:JW3953
eco:b3990
PATRICifig|1411691.4.peg.2722

Similar proteinsi

Entry informationi

Entry nameiTHIH_ECOLI
AccessioniPrimary (citable) accession number: P30140
Secondary accession number(s): Q2M8S6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 137 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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