Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Carbamoyltransferase HypF

Gene

hypF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Along with HypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carboxamido moiety in an ATP-dependent reaction to the thiolate of the C-terminal cysteine of HypE yielding a protein-S-carboxamide.4 Publications

Pathwayi: [NiFe] hydrogenase maturation

This protein is involved in the pathway [NiFe] hydrogenase maturation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway [NiFe] hydrogenase maturation and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri109 – 13426C4-typeSequence analysisAdd
BLAST
Zinc fingeri159 – 18426C4-typeSequence analysisAdd
BLAST

GO - Molecular functioni

  • carboxyl- or carbamoyltransferase activity Source: EcoCyc
  • double-stranded RNA binding Source: InterPro
  • zinc ion binding Source: EcoCyc

GO - Biological processi

  • protein carbamoylation Source: EcoCyc
  • protein maturation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:EG11551-MONOMER.
ECOL316407:JW5433-MONOMER.
MetaCyc:EG11551-MONOMER.
UniPathwayiUPA00335.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyltransferase HypF (EC:2.1.3.-)
Alternative name(s):
Carbamoyl phosphate-converting enzyme HypF
[NiFe]-hydrogenase maturation factor HypF
Short name:
Hydrogenase maturation protein HypF
Gene namesi
Name:hypF
Synonyms:hydA
Ordered Locus Names:b2712, JW5433
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11551. hypF.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi23 – 231R → E, H or K: Loss of activity. 1 Publication
Mutagenesisi23 – 231R → Q: Diminishes activity. 1 Publication
Mutagenesisi112 – 1121C → A: Loss of activity. 1 Publication
Mutagenesisi162 – 1621C → A: Loss of activity. 1 Publication
Mutagenesisi475 – 4751H → A: Loss of activity. 1 Publication
Mutagenesisi476 – 4761H → A: Carbamoyl phosphate-dependent ATP hydrolysis activity diminished in vitro but sufficient for wild-type-like phenotype. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 750750Carbamoyltransferase HypFPRO_0000071614Add
BLAST

Proteomic databases

PaxDbiP30131.
PRIDEiP30131.

Interactioni

Subunit structurei

Forms a complex with HypE.1 Publication

Protein-protein interaction databases

BioGridi4259427. 14 interactions.
DIPiDIP-10000N.
IntActiP30131. 8 interactions.
MINTiMINT-1276849.
STRINGi511145.b2712.

Structurei

Secondary structure

1
750
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 1611Combined sources
Beta strandi18 – 214Combined sources
Helixi22 – 3312Combined sources
Beta strandi37 – 415Combined sources
Beta strandi43 – 5210Combined sources
Helixi55 – 639Combined sources
Beta strandi70 – 8314Combined sources
Beta strandi86 – 905Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GXTX-ray1.30A1-91[»]
1GXUX-ray1.27A1-91[»]
ProteinModelPortaliP30131.
SMRiP30131. Positions 4-91, 101-746.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP30131.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 9285Acylphosphatase-likePROSITE-ProRule annotationAdd
BLAST
Domaini200 – 376177YrdC-likePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni19 – 235Substrate binding

Domaini

Contains a version of the YrdC-like domain which probably has a different function than that of some proteins where it has been implicated in RNA binding.
Contains a unique C-terminal domain with an O-carbamoyltransferase motif.

Sequence similaritiesi

Belongs to the carbamoyltransferase HypF family.Curated
Contains 1 acylphosphatase-like domain.PROSITE-ProRule annotation
Contains 1 YrdC-like domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri109 – 13426C4-typeSequence analysisAdd
BLAST
Zinc fingeri159 – 18426C4-typeSequence analysisAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG4105C8H. Bacteria.
COG0068. LUCA.
HOGENOMiHOG000278743.
InParanoidiP30131.
KOiK04656.
OMAiYVMTSAN.
PhylomeDBiP30131.

Family and domain databases

Gene3Di3.90.870.10. 1 hit.
InterProiIPR001792. Acylphosphatase-like_dom.
IPR017968. Acylphosphatase_CS.
IPR004421. Carbamoyltransferase_HypF.
IPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR006070. YrdC-like_dom.
IPR011125. Znf_HypF.
[Graphical view]
PfamiPF00708. Acylphosphatase. 1 hit.
PF01300. Sua5_yciO_yrdC. 1 hit.
PF07503. zf-HYPF. 2 hits.
[Graphical view]
PIRSFiPIRSF006256. CMPcnvr_hdrg_mat. 1 hit.
SUPFAMiSSF54975. SSF54975. 1 hit.
SSF55821. SSF55821. 1 hit.
TIGRFAMsiTIGR00143. hypF. 1 hit.
PROSITEiPS00150. ACYLPHOSPHATASE_1. 1 hit.
PS51160. ACYLPHOSPHATASE_3. 1 hit.
PS51163. YRDC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30131-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKNTSCGVQ LRIRGKVQGV GFRPFVWQLA QQLNLHGDVC NDGDGVEVRL
60 70 80 90 100
REDPETFLVQ LYQHCPPLAR IDSVEREPFI WSQLPTEFTI RQSTGGTMNT
110 120 130 140 150
QIVPDAATCP ACLAEMNTPG ERRYRYPFIN CTHCGPRFTI IRAMPYDRPF
160 170 180 190 200
TVMAAFPLCP ACDKEYRDPL DRRFHAQPVA CPECGPHLEW VSHGEHAEQE
210 220 230 240 250
AALQAAIAQL KMGKIVAIKG IGGFHLACDA RNSNAVATLR ARKHRPAKPL
260 270 280 290 300
AVMLPVADGL PDAARQLLTT PAAPIVLVDK KYVPELCDDI APDLNEVGVM
310 320 330 340 350
LPANPLQHLL LQELQCPLVM TSGNLSGKPP AISNEQALAD LQGIADGFLI
360 370 380 390 400
HNRDIVQRMD DSVVRESGEM LRRSRGYVPD ALALPPGFKN VPPVLCLGAD
410 420 430 440 450
LKNTFCLVRG EQAVLSQHLG DLSDDGIQMQ WREALRLMQN IYDFTPQYVV
460 470 480 490 500
HDAHPGYVSS QWAREMNLPT QTVLHHHAHA AACLAEHQWP LDGGDVIALT
510 520 530 540 550
LDGIGMGENG ALWGGECLRV NYRECEHLGG LPAVALPGGD LAAKQPWRNL
560 570 580 590 600
LAQCLRFVPE WQNYSETASV QQQNWSVLAR AIERGINAPL ASSCGRFFDA
610 620 630 640 650
VAAALGCAPA TLSYEGEAAC ALEALAASCH GVTHPVTMPR VDNQLDLATF
660 670 680 690 700
WQQWLNWQAP VNQRAWAFHD ALAQGFAALM REQATMRGIT TLVFSGGVIH
710 720 730 740 750
NRLLRARLAH YLADFTLLFP QSLPAGDGGL SLGQGVIAAA RWLAGEVQNG
Length:750
Mass (Da):82,066
Last modified:November 1, 1997 - v2
Checksum:i8BB245F80349A91E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti31 – 322QQ → RE in BAA03315 (Ref. 1) Curated
Sequence conflicti367 – 38620SGEML…LALPP → RRNAAPFAGVCAGCAGFAS in BAA03315 (Ref. 1) CuratedAdd
BLAST
Sequence conflicti431 – 4322WR → CA in BAA03315 (Ref. 1) Curated
Sequence conflicti435 – 4351L → S in BAA03315 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14422 Genomic DNA. Translation: BAA03315.1.
U29579 Genomic DNA. Translation: AAA69222.1.
U00096 Genomic DNA. Translation: AAC75754.1.
AP009048 Genomic DNA. Translation: BAE76789.1.
PIRiD65051.
RefSeqiNP_417192.1. NC_000913.3.
WP_001107704.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75754; AAC75754; b2712.
BAE76789; BAE76789; BAE76789.
GeneIDi944963.
KEGGiecj:JW5433.
eco:b2712.
PATRICi32120822. VBIEscCol129921_2803.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14422 Genomic DNA. Translation: BAA03315.1.
U29579 Genomic DNA. Translation: AAA69222.1.
U00096 Genomic DNA. Translation: AAC75754.1.
AP009048 Genomic DNA. Translation: BAE76789.1.
PIRiD65051.
RefSeqiNP_417192.1. NC_000913.3.
WP_001107704.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GXTX-ray1.30A1-91[»]
1GXUX-ray1.27A1-91[»]
ProteinModelPortaliP30131.
SMRiP30131. Positions 4-91, 101-746.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259427. 14 interactions.
DIPiDIP-10000N.
IntActiP30131. 8 interactions.
MINTiMINT-1276849.
STRINGi511145.b2712.

Proteomic databases

PaxDbiP30131.
PRIDEiP30131.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75754; AAC75754; b2712.
BAE76789; BAE76789; BAE76789.
GeneIDi944963.
KEGGiecj:JW5433.
eco:b2712.
PATRICi32120822. VBIEscCol129921_2803.

Organism-specific databases

EchoBASEiEB1512.
EcoGeneiEG11551. hypF.

Phylogenomic databases

eggNOGiENOG4105C8H. Bacteria.
COG0068. LUCA.
HOGENOMiHOG000278743.
InParanoidiP30131.
KOiK04656.
OMAiYVMTSAN.
PhylomeDBiP30131.

Enzyme and pathway databases

UniPathwayiUPA00335.
BioCyciEcoCyc:EG11551-MONOMER.
ECOL316407:JW5433-MONOMER.
MetaCyc:EG11551-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP30131.
PROiP30131.

Family and domain databases

Gene3Di3.90.870.10. 1 hit.
InterProiIPR001792. Acylphosphatase-like_dom.
IPR017968. Acylphosphatase_CS.
IPR004421. Carbamoyltransferase_HypF.
IPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR006070. YrdC-like_dom.
IPR011125. Znf_HypF.
[Graphical view]
PfamiPF00708. Acylphosphatase. 1 hit.
PF01300. Sua5_yciO_yrdC. 1 hit.
PF07503. zf-HYPF. 2 hits.
[Graphical view]
PIRSFiPIRSF006256. CMPcnvr_hdrg_mat. 1 hit.
SUPFAMiSSF54975. SSF54975. 1 hit.
SSF55821. SSF55821. 1 hit.
TIGRFAMsiTIGR00143. hypF. 1 hit.
PROSITEiPS00150. ACYLPHOSPHATASE_1. 1 hit.
PS51160. ACYLPHOSPHATASE_3. 1 hit.
PS51163. YRDC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHYPF_ECOLI
AccessioniPrimary (citable) accession number: P30131
Secondary accession number(s): Q2MAB7, Q46878
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.