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Protein

Carbamoyltransferase HypF

Gene

hypF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the maturation of [NiFe] hydrogenases (PubMed:8661925, PubMed:12377778, PubMed:12586941). Along with HypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases (PubMed:12586941). HypF functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carboxamido moiety in an ATP-dependent reaction to the thiolate of the C-terminal cysteine of HypE yielding a protein-S-carboxamide (PubMed:12586941, PubMed:15291820). In the absence of any other substrate, displays carbamoyl-phosphate phosphatase activity (PubMed:12377778).4 Publications

Catalytic activityi

ATP + C-terminal L-cysteinyl-[HypE protein] + carbamoyl phosphate + H2O = AMP + C-terminal S-carboxamide-L-cysteinyl-[HypE protein] + diphosphate + phosphate.2 Publications

Pathwayi: [NiFe] hydrogenase maturation

This protein is involved in the pathway [NiFe] hydrogenase maturation, which is part of Protein modification.Curated
View all proteins of this organism that are known to be involved in the pathway [NiFe] hydrogenase maturation and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri109 – 134C4-type1 PublicationAdd BLAST26
Zinc fingeri159 – 184C4-type1 PublicationAdd BLAST26

GO - Molecular functioni

  • carboxyl- or carbamoyltransferase activity Source: EcoCyc
  • double-stranded RNA binding Source: InterPro
  • ligase activity Source: UniProtKB-KW
  • zinc ion binding Source: EcoCyc

GO - Biological processi

  • protein carbamoylation Source: EcoCyc
  • protein maturation Source: EcoCyc

Keywordsi

Molecular functionLigase
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:EG11551-MONOMER
MetaCyc:EG11551-MONOMER
UniPathwayiUPA00335

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyltransferase HypFCurated (EC:6.2.-.-2 Publications)
Alternative name(s):
Carbamoyl phosphate-converting enzyme HypF
[NiFe]-hydrogenase maturation factor HypF
Short name:
Hydrogenase maturation protein HypF
Gene namesi
Name:hypF1 Publication
Synonyms:hydA1 Publication
Ordered Locus Names:b2712, JW5433
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11551 hypF

Pathology & Biotechi

Disruption phenotypei

Deletion of the gene results in the loss of hydrogenase activity and in the synthesis of the large subunits of the hydrogenase 1, 2 and 3 in the inactive precursor form.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi23R → E or H: Loss of activity. 2 Publications1
Mutagenesisi23R → Q: Diminishes activity. 2 Publications1
Mutagenesisi109C → A: Loss of activity. 1 Publication1
Mutagenesisi159C → A: Loss of activity. 1 Publication1
Mutagenesisi475H → A: No change in activity. 1 Publication1
Mutagenesisi476H → A: Carbamoyl phosphate-dependent ATP hydrolysis activity diminished in vitro but sufficient for wild-type-like phenotype. 2 Publications1
Mutagenesisi479H → A: No change in activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000716141 – 750Carbamoyltransferase HypFAdd BLAST750

Proteomic databases

PaxDbiP30131
PRIDEiP30131

Interactioni

Subunit structurei

Monomer (PubMed:12377778). Forms a complex with HypE (PubMed:15291820, PubMed:18065529).3 Publications

Protein-protein interaction databases

BioGridi4259427, 14 interactors
DIPiDIP-10000N
IntActiP30131, 8 interactors
STRINGi316385.ECDH10B_2880

Structurei

Secondary structure

1750
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 16Combined sources11
Beta strandi18 – 21Combined sources4
Helixi22 – 33Combined sources12
Beta strandi37 – 41Combined sources5
Beta strandi43 – 52Combined sources10
Helixi55 – 63Combined sources9
Beta strandi70 – 83Combined sources14
Beta strandi86 – 90Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GXTX-ray1.30A1-91[»]
1GXUX-ray1.27A1-91[»]
ProteinModelPortaliP30131
SMRiP30131
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP30131

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 92Acylphosphatase-likePROSITE-ProRule annotationAdd BLAST85
Domaini200 – 376YrdC-likePROSITE-ProRule annotationAdd BLAST177

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni19 – 23Substrate binding1 Publication5

Domaini

Contains a version of the YrdC-like domain which probably has a different function than that of some proteins where it has been implicated in RNA binding.
Contains a unique C-terminal domain with an O-carbamoyltransferase motif.

Sequence similaritiesi

Belongs to the carbamoyltransferase HypF family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri109 – 134C4-type1 PublicationAdd BLAST26
Zinc fingeri159 – 184C4-type1 PublicationAdd BLAST26

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG4105C8H Bacteria
COG0068 LUCA
HOGENOMiHOG000278743
InParanoidiP30131
KOiK04656
OMAiVQHHYAH
PhylomeDBiP30131

Family and domain databases

InterProiView protein in InterPro
IPR001792 Acylphosphatase-like_dom
IPR036046 Acylphosphatase-like_dom_sf
IPR017968 Acylphosphatase_CS
IPR004421 Carbamoyltransferase_HypF
IPR017945 DHBP_synth_RibB-like_a/b_dom
IPR006070 YrdC-like_dom
IPR011125 Znf_HypF
PfamiView protein in Pfam
PF00708 Acylphosphatase, 1 hit
PF01300 Sua5_yciO_yrdC, 1 hit
PF07503 zf-HYPF, 2 hits
PIRSFiPIRSF006256 CMPcnvr_hdrg_mat, 1 hit
SUPFAMiSSF54975 SSF54975, 1 hit
SSF55821 SSF55821, 1 hit
TIGRFAMsiTIGR00143 hypF, 1 hit
PROSITEiView protein in PROSITE
PS00150 ACYLPHOSPHATASE_1, 1 hit
PS51160 ACYLPHOSPHATASE_3, 1 hit
PS51163 YRDC, 1 hit

Sequencei

Sequence statusi: Complete.

P30131-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKNTSCGVQ LRIRGKVQGV GFRPFVWQLA QQLNLHGDVC NDGDGVEVRL
60 70 80 90 100
REDPETFLVQ LYQHCPPLAR IDSVEREPFI WSQLPTEFTI RQSTGGTMNT
110 120 130 140 150
QIVPDAATCP ACLAEMNTPG ERRYRYPFIN CTHCGPRFTI IRAMPYDRPF
160 170 180 190 200
TVMAAFPLCP ACDKEYRDPL DRRFHAQPVA CPECGPHLEW VSHGEHAEQE
210 220 230 240 250
AALQAAIAQL KMGKIVAIKG IGGFHLACDA RNSNAVATLR ARKHRPAKPL
260 270 280 290 300
AVMLPVADGL PDAARQLLTT PAAPIVLVDK KYVPELCDDI APDLNEVGVM
310 320 330 340 350
LPANPLQHLL LQELQCPLVM TSGNLSGKPP AISNEQALAD LQGIADGFLI
360 370 380 390 400
HNRDIVQRMD DSVVRESGEM LRRSRGYVPD ALALPPGFKN VPPVLCLGAD
410 420 430 440 450
LKNTFCLVRG EQAVLSQHLG DLSDDGIQMQ WREALRLMQN IYDFTPQYVV
460 470 480 490 500
HDAHPGYVSS QWAREMNLPT QTVLHHHAHA AACLAEHQWP LDGGDVIALT
510 520 530 540 550
LDGIGMGENG ALWGGECLRV NYRECEHLGG LPAVALPGGD LAAKQPWRNL
560 570 580 590 600
LAQCLRFVPE WQNYSETASV QQQNWSVLAR AIERGINAPL ASSCGRFFDA
610 620 630 640 650
VAAALGCAPA TLSYEGEAAC ALEALAASCH GVTHPVTMPR VDNQLDLATF
660 670 680 690 700
WQQWLNWQAP VNQRAWAFHD ALAQGFAALM REQATMRGIT TLVFSGGVIH
710 720 730 740 750
NRLLRARLAH YLADFTLLFP QSLPAGDGGL SLGQGVIAAA RWLAGEVQNG
Length:750
Mass (Da):82,066
Last modified:November 1, 1997 - v2
Checksum:i8BB245F80349A91E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti31 – 32QQ → RE in BAA03315 (Ref. 1) Curated2
Sequence conflicti367 – 386SGEML…LALPP → RRNAAPFAGVCAGCAGFAS in BAA03315 (Ref. 1) CuratedAdd BLAST20
Sequence conflicti431 – 432WR → CA in BAA03315 (Ref. 1) Curated2
Sequence conflicti435L → S in BAA03315 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14422 Genomic DNA Translation: BAA03315.1
U29579 Genomic DNA Translation: AAA69222.1
U00096 Genomic DNA Translation: AAC75754.1
AP009048 Genomic DNA Translation: BAE76789.1
PIRiD65051
RefSeqiNP_417192.1, NC_000913.3
WP_001107704.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75754; AAC75754; b2712
BAE76789; BAE76789; BAE76789
GeneIDi944963
KEGGiecj:JW5433
eco:b2712
PATRICifig|1411691.4.peg.4030

Similar proteinsi

Entry informationi

Entry nameiHYPF_ECOLI
AccessioniPrimary (citable) accession number: P30131
Secondary accession number(s): Q2MAB7, Q46878
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 152 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health