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Protein

Carbamoyltransferase HypF

Gene

hypF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Along with HypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carboxamido moiety in an ATP-dependent reaction to the thiolate of the C-terminal cysteine of HypE yielding a protein-S-carboxamide.4 Publications

Pathwayi: [NiFe] hydrogenase maturation

This protein is involved in the pathway [NiFe] hydrogenase maturation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway [NiFe] hydrogenase maturation and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri109 – 134C4-typeSequence analysisAdd BLAST26
Zinc fingeri159 – 184C4-typeSequence analysisAdd BLAST26

GO - Molecular functioni

  • carboxyl- or carbamoyltransferase activity Source: EcoCyc
  • double-stranded RNA binding Source: InterPro
  • zinc ion binding Source: EcoCyc

GO - Biological processi

  • protein carbamoylation Source: EcoCyc
  • protein maturation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:EG11551-MONOMER.
ECOL316407:JW5433-MONOMER.
MetaCyc:EG11551-MONOMER.
UniPathwayiUPA00335.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyltransferase HypF (EC:2.1.3.-)
Alternative name(s):
Carbamoyl phosphate-converting enzyme HypF
[NiFe]-hydrogenase maturation factor HypF
Short name:
Hydrogenase maturation protein HypF
Gene namesi
Name:hypF
Synonyms:hydA
Ordered Locus Names:b2712, JW5433
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11551. hypF.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi23R → E, H or K: Loss of activity. 1 Publication1
Mutagenesisi23R → Q: Diminishes activity. 1 Publication1
Mutagenesisi112C → A: Loss of activity. 1 Publication1
Mutagenesisi162C → A: Loss of activity. 1 Publication1
Mutagenesisi475H → A: Loss of activity. 1 Publication1
Mutagenesisi476H → A: Carbamoyl phosphate-dependent ATP hydrolysis activity diminished in vitro but sufficient for wild-type-like phenotype. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000716141 – 750Carbamoyltransferase HypFAdd BLAST750

Proteomic databases

PaxDbiP30131.
PRIDEiP30131.

Interactioni

Subunit structurei

Forms a complex with HypE.1 Publication

Protein-protein interaction databases

BioGridi4259427. 14 interactors.
DIPiDIP-10000N.
IntActiP30131. 8 interactors.
MINTiMINT-1276849.
STRINGi511145.b2712.

Structurei

Secondary structure

1750
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 16Combined sources11
Beta strandi18 – 21Combined sources4
Helixi22 – 33Combined sources12
Beta strandi37 – 41Combined sources5
Beta strandi43 – 52Combined sources10
Helixi55 – 63Combined sources9
Beta strandi70 – 83Combined sources14
Beta strandi86 – 90Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GXTX-ray1.30A1-91[»]
1GXUX-ray1.27A1-91[»]
ProteinModelPortaliP30131.
SMRiP30131.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP30131.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 92Acylphosphatase-likePROSITE-ProRule annotationAdd BLAST85
Domaini200 – 376YrdC-likePROSITE-ProRule annotationAdd BLAST177

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni19 – 23Substrate binding5

Domaini

Contains a version of the YrdC-like domain which probably has a different function than that of some proteins where it has been implicated in RNA binding.
Contains a unique C-terminal domain with an O-carbamoyltransferase motif.

Sequence similaritiesi

Belongs to the carbamoyltransferase HypF family.Curated
Contains 1 acylphosphatase-like domain.PROSITE-ProRule annotation
Contains 1 YrdC-like domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri109 – 134C4-typeSequence analysisAdd BLAST26
Zinc fingeri159 – 184C4-typeSequence analysisAdd BLAST26

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG4105C8H. Bacteria.
COG0068. LUCA.
HOGENOMiHOG000278743.
InParanoidiP30131.
KOiK04656.
OMAiYVMTSAN.
PhylomeDBiP30131.

Family and domain databases

Gene3Di3.90.870.10. 1 hit.
InterProiIPR001792. Acylphosphatase-like_dom.
IPR017968. Acylphosphatase_CS.
IPR004421. Carbamoyltransferase_HypF.
IPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR006070. YrdC-like_dom.
IPR011125. Znf_HypF.
[Graphical view]
PfamiPF00708. Acylphosphatase. 1 hit.
PF01300. Sua5_yciO_yrdC. 1 hit.
PF07503. zf-HYPF. 2 hits.
[Graphical view]
PIRSFiPIRSF006256. CMPcnvr_hdrg_mat. 1 hit.
SUPFAMiSSF54975. SSF54975. 1 hit.
SSF55821. SSF55821. 1 hit.
TIGRFAMsiTIGR00143. hypF. 1 hit.
PROSITEiPS00150. ACYLPHOSPHATASE_1. 1 hit.
PS51160. ACYLPHOSPHATASE_3. 1 hit.
PS51163. YRDC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30131-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKNTSCGVQ LRIRGKVQGV GFRPFVWQLA QQLNLHGDVC NDGDGVEVRL
60 70 80 90 100
REDPETFLVQ LYQHCPPLAR IDSVEREPFI WSQLPTEFTI RQSTGGTMNT
110 120 130 140 150
QIVPDAATCP ACLAEMNTPG ERRYRYPFIN CTHCGPRFTI IRAMPYDRPF
160 170 180 190 200
TVMAAFPLCP ACDKEYRDPL DRRFHAQPVA CPECGPHLEW VSHGEHAEQE
210 220 230 240 250
AALQAAIAQL KMGKIVAIKG IGGFHLACDA RNSNAVATLR ARKHRPAKPL
260 270 280 290 300
AVMLPVADGL PDAARQLLTT PAAPIVLVDK KYVPELCDDI APDLNEVGVM
310 320 330 340 350
LPANPLQHLL LQELQCPLVM TSGNLSGKPP AISNEQALAD LQGIADGFLI
360 370 380 390 400
HNRDIVQRMD DSVVRESGEM LRRSRGYVPD ALALPPGFKN VPPVLCLGAD
410 420 430 440 450
LKNTFCLVRG EQAVLSQHLG DLSDDGIQMQ WREALRLMQN IYDFTPQYVV
460 470 480 490 500
HDAHPGYVSS QWAREMNLPT QTVLHHHAHA AACLAEHQWP LDGGDVIALT
510 520 530 540 550
LDGIGMGENG ALWGGECLRV NYRECEHLGG LPAVALPGGD LAAKQPWRNL
560 570 580 590 600
LAQCLRFVPE WQNYSETASV QQQNWSVLAR AIERGINAPL ASSCGRFFDA
610 620 630 640 650
VAAALGCAPA TLSYEGEAAC ALEALAASCH GVTHPVTMPR VDNQLDLATF
660 670 680 690 700
WQQWLNWQAP VNQRAWAFHD ALAQGFAALM REQATMRGIT TLVFSGGVIH
710 720 730 740 750
NRLLRARLAH YLADFTLLFP QSLPAGDGGL SLGQGVIAAA RWLAGEVQNG
Length:750
Mass (Da):82,066
Last modified:November 1, 1997 - v2
Checksum:i8BB245F80349A91E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti31 – 32QQ → RE in BAA03315 (Ref. 1) Curated2
Sequence conflicti367 – 386SGEML…LALPP → RRNAAPFAGVCAGCAGFAS in BAA03315 (Ref. 1) CuratedAdd BLAST20
Sequence conflicti431 – 432WR → CA in BAA03315 (Ref. 1) Curated2
Sequence conflicti435L → S in BAA03315 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14422 Genomic DNA. Translation: BAA03315.1.
U29579 Genomic DNA. Translation: AAA69222.1.
U00096 Genomic DNA. Translation: AAC75754.1.
AP009048 Genomic DNA. Translation: BAE76789.1.
PIRiD65051.
RefSeqiNP_417192.1. NC_000913.3.
WP_001107704.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75754; AAC75754; b2712.
BAE76789; BAE76789; BAE76789.
GeneIDi944963.
KEGGiecj:JW5433.
eco:b2712.
PATRICi32120822. VBIEscCol129921_2803.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14422 Genomic DNA. Translation: BAA03315.1.
U29579 Genomic DNA. Translation: AAA69222.1.
U00096 Genomic DNA. Translation: AAC75754.1.
AP009048 Genomic DNA. Translation: BAE76789.1.
PIRiD65051.
RefSeqiNP_417192.1. NC_000913.3.
WP_001107704.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GXTX-ray1.30A1-91[»]
1GXUX-ray1.27A1-91[»]
ProteinModelPortaliP30131.
SMRiP30131.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259427. 14 interactors.
DIPiDIP-10000N.
IntActiP30131. 8 interactors.
MINTiMINT-1276849.
STRINGi511145.b2712.

Proteomic databases

PaxDbiP30131.
PRIDEiP30131.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75754; AAC75754; b2712.
BAE76789; BAE76789; BAE76789.
GeneIDi944963.
KEGGiecj:JW5433.
eco:b2712.
PATRICi32120822. VBIEscCol129921_2803.

Organism-specific databases

EchoBASEiEB1512.
EcoGeneiEG11551. hypF.

Phylogenomic databases

eggNOGiENOG4105C8H. Bacteria.
COG0068. LUCA.
HOGENOMiHOG000278743.
InParanoidiP30131.
KOiK04656.
OMAiYVMTSAN.
PhylomeDBiP30131.

Enzyme and pathway databases

UniPathwayiUPA00335.
BioCyciEcoCyc:EG11551-MONOMER.
ECOL316407:JW5433-MONOMER.
MetaCyc:EG11551-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP30131.
PROiP30131.

Family and domain databases

Gene3Di3.90.870.10. 1 hit.
InterProiIPR001792. Acylphosphatase-like_dom.
IPR017968. Acylphosphatase_CS.
IPR004421. Carbamoyltransferase_HypF.
IPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR006070. YrdC-like_dom.
IPR011125. Znf_HypF.
[Graphical view]
PfamiPF00708. Acylphosphatase. 1 hit.
PF01300. Sua5_yciO_yrdC. 1 hit.
PF07503. zf-HYPF. 2 hits.
[Graphical view]
PIRSFiPIRSF006256. CMPcnvr_hdrg_mat. 1 hit.
SUPFAMiSSF54975. SSF54975. 1 hit.
SSF55821. SSF55821. 1 hit.
TIGRFAMsiTIGR00143. hypF. 1 hit.
PROSITEiPS00150. ACYLPHOSPHATASE_1. 1 hit.
PS51160. ACYLPHOSPHATASE_3. 1 hit.
PS51163. YRDC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHYPF_ECOLI
AccessioniPrimary (citable) accession number: P30131
Secondary accession number(s): Q2MAB7, Q46878
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 145 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.