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Protein

Outer membrane usher protein FimD

Gene

fimD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the export and assembly of FimA fimbrial subunits across the outer membrane.

GO - Molecular functioni

  • fimbrial usher porin activity Source: EcoCyc

GO - Biological processi

  • pilus assembly Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Fimbrium biogenesis, Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG10311-MONOMER.
ECOL316407:JW5780-MONOMER.

Protein family/group databases

TCDBi1.B.11.3.9. the outer membrane fimbrial usher porin (fup) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Outer membrane usher protein FimD
Gene namesi
Name:fimD
Ordered Locus Names:b4317, JW5780
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10311. fimD.

Subcellular locationi

GO - Cellular componenti

  • cell outer membrane Source: EcoCyc
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 451 PublicationAdd BLAST45
ChainiPRO_000000931246 – 878Outer membrane usher protein FimDAdd BLAST833

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi855 ↔ 877Sequence analysis

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP30130.
PRIDEiP30130.

Interactioni

Protein-protein interaction databases

BioGridi4262748. 186 interactors.
DIPiDIP-9612N.
IntActiP30130. 7 interactors.
MINTiMINT-1292016.
STRINGi511145.b4317.

Structurei

Secondary structure

1878
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi51 – 54Combined sources4
Helixi60 – 68Combined sources9
Beta strandi75 – 84Combined sources10
Beta strandi87 – 98Combined sources12
Beta strandi104 – 107Combined sources4
Helixi111 – 116Combined sources6
Helixi121 – 123Combined sources3
Helixi127 – 129Combined sources3
Helixi138 – 141Combined sources4
Turni142 – 144Combined sources3
Beta strandi146 – 150Combined sources5
Turni151 – 154Combined sources4
Beta strandi155 – 159Combined sources5
Helixi162 – 164Combined sources3
Helixi175 – 177Combined sources3
Beta strandi184 – 198Combined sources15
Turni199 – 201Combined sources3
Beta strandi202 – 217Combined sources16
Beta strandi220 – 231Combined sources12
Beta strandi243 – 254Combined sources12
Turni255 – 258Combined sources4
Beta strandi259 – 267Combined sources9
Beta strandi270 – 273Combined sources4
Beta strandi277 – 285Combined sources9
Helixi287 – 289Combined sources3
Helixi292 – 294Combined sources3
Beta strandi301 – 315Combined sources15
Beta strandi318 – 325Combined sources8
Beta strandi327 – 332Combined sources6
Turni338 – 341Combined sources4
Beta strandi344 – 349Combined sources6
Beta strandi351 – 353Combined sources3
Beta strandi355 – 360Combined sources6
Beta strandi374 – 383Combined sources10
Beta strandi394 – 403Combined sources10
Helixi405 – 407Combined sources3
Beta strandi408 – 417Combined sources10
Beta strandi420 – 432Combined sources13
Beta strandi435 – 447Combined sources13
Beta strandi453 – 465Combined sources13
Beta strandi471 – 482Combined sources12
Helixi489 – 493Combined sources5
Beta strandi494 – 496Combined sources3
Beta strandi525 – 536Combined sources12
Beta strandi538 – 554Combined sources17
Beta strandi560 – 570Combined sources11
Beta strandi573 – 583Combined sources11
Beta strandi585 – 587Combined sources3
Beta strandi591 – 602Combined sources12
Helixi603 – 605Combined sources3
Helixi613 – 616Combined sources4
Beta strandi617 – 625Combined sources9
Beta strandi627 – 629Combined sources3
Beta strandi631 – 642Combined sources12
Turni643 – 646Combined sources4
Beta strandi647 – 658Combined sources12
Turni659 – 661Combined sources3
Beta strandi664 – 674Combined sources11
Beta strandi679 – 686Combined sources8
Beta strandi691 – 704Combined sources14
Beta strandi707 – 712Combined sources6
Beta strandi716 – 722Combined sources7
Beta strandi743 – 748Combined sources6
Beta strandi756 – 760Combined sources5
Helixi762 – 764Combined sources3
Beta strandi769 – 772Combined sources4
Beta strandi774 – 778Combined sources5
Beta strandi785 – 789Combined sources5
Beta strandi792 – 804Combined sources13
Beta strandi814 – 821Combined sources8
Beta strandi823 – 825Combined sources3
Helixi828 – 830Combined sources3
Beta strandi831 – 837Combined sources7
Beta strandi839 – 847Combined sources9
Beta strandi856 – 860Combined sources5
Helixi863 – 865Combined sources3
Beta strandi871 – 875Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZDVNMR-A70-184[»]
1ZDXNMR-A70-170[»]
1ZE3X-ray1.84D46-170[»]
3BWUX-ray1.76D46-170[»]
3OHNX-ray3.01A/B169-708[»]
3RFZX-ray2.80B/E46-878[»]
4J3OX-ray3.80D46-878[»]
ProteinModelPortaliP30130.
SMRiP30130.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP30130.

Family & Domainsi

Sequence similaritiesi

Belongs to the fimbrial export usher family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4105CW0. Bacteria.
COG3188. LUCA.
HOGENOMiHOG000111414.
InParanoidiP30130.
KOiK07347.
OMAiVPQAFMG.
PhylomeDBiP30130.

Family and domain databases

InterProiIPR000015. Fimb_usher.
IPR018030. Fimbrial_membr_usher_CS.
IPR025949. PapC-like_C.
IPR025885. PapC_N.
[Graphical view]
PANTHERiPTHR30451. PTHR30451. 1 hit.
PfamiPF13953. PapC_C. 1 hit.
PF13954. PapC_N. 1 hit.
PF00577. Usher. 1 hit.
[Graphical view]
PROSITEiPS01151. FIMBRIAL_USHER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30130-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYLNLRLYQ RNTQCLHIRK HRLAGFFVRL VVACAFAAQA PLSSADLYFN
60 70 80 90 100
PRFLADDPQA VADLSRFENG QELPPGTYRV DIYLNNGYMA TRDVTFNTGD
110 120 130 140 150
SEQGIVPCLT RAQLASMGLN TASVAGMNLL ADDACVPLTT MVQDATAHLD
160 170 180 190 200
VGQQRLNLTI PQAFMSNRAR GYIPPELWDP GINAGLLNYN FSGNSVQNRI
210 220 230 240 250
GGNSHYAYLN LQSGLNIGAW RLRDNTTWSY NSSDRSSGSK NKWQHINTWL
260 270 280 290 300
ERDIIPLRSR LTLGDGYTQG DIFDGINFRG AQLASDDNML PDSQRGFAPV
310 320 330 340 350
IHGIARGTAQ VTIKQNGYDI YNSTVPPGPF TINDIYAAGN SGDLQVTIKE
360 370 380 390 400
ADGSTQIFTV PYSSVPLLQR EGHTRYSITA GEYRSGNAQQ EKTRFFQSTL
410 420 430 440 450
LHGLPAGWTI YGGTQLADRY RAFNFGIGKN MGALGALSVD MTQANSTLPD
460 470 480 490 500
DSQHDGQSVR FLYNKSLNES GTNIQLVGYR YSTSGYFNFA DTTYSRMNGY
510 520 530 540 550
NIETQDGVIQ VKPKFTDYYN LAYNKRGKLQ LTVTQQLGRT STLYLSGSHQ
560 570 580 590 600
TYWGTSNVDE QFQAGLNTAF EDINWTLSYS LTKNAWQKGR DQMLALNVNI
610 620 630 640 650
PFSHWLRSDS KSQWRHASAS YSMSHDLNGR MTNLAGVYGT LLEDNNLSYS
660 670 680 690 700
VQTGYAGGGD GNSGSTGYAT LNYRGGYGNA NIGYSHSDDI KQLYYGVSGG
710 720 730 740 750
VLAHANGVTL GQPLNDTVVL VKAPGAKDAK VENQTGVRTD WRGYAVLPYA
760 770 780 790 800
TEYRENRVAL DTNTLADNVD LDNAVANVVP TRGAIVRAEF KARVGIKLLM
810 820 830 840 850
TLTHNNKPLP FGAMVTSESS QSSGIVADNG QVYLSGMPLA GKVQVKWGEE
860 870
ENAHCVANYQ LPPESQQQLL TQLSAECR
Length:878
Mass (Da):96,482
Last modified:February 1, 1995 - v2
Checksum:iB2B86CC44FAB7B0F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti143 – 148QDATAH → RTLLRT in CAA35968 (PubMed:1970114).Curated6
Sequence conflicti352 – 402DGSTQ…STLLH → VLAARRFLPYPIRTFPLLNV KAYSLFHYGRRIPYESRSRK KPAFSRVHYSN (PubMed:1970114).CuratedAdd BLAST51
Sequence conflicti658 – 670GGDGN…TGYAT → EAMEIAEVQATP (PubMed:1970114).CuratedAdd BLAST13
Sequence conflicti723 – 742APGAK…RTDWR → RLAQKMQKSKTRRGAYRLA in CAA35968 (PubMed:1970114).CuratedAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51655 Genomic DNA. Translation: CAA35968.1.
U14003 Genomic DNA. Translation: AAA97213.1.
U00096 Genomic DNA. Translation: AAC77273.1.
AP009048 Genomic DNA. Translation: BAE78310.1.
PIRiS56542.
RefSeqiNP_418737.1. NC_000913.3.
WP_000121005.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77273; AAC77273; b4317.
BAE78310; BAE78310; BAE78310.
GeneIDi948844.
KEGGiecj:JW5780.
eco:b4317.
PATRICi32124230. VBIEscCol129921_4458.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51655 Genomic DNA. Translation: CAA35968.1.
U14003 Genomic DNA. Translation: AAA97213.1.
U00096 Genomic DNA. Translation: AAC77273.1.
AP009048 Genomic DNA. Translation: BAE78310.1.
PIRiS56542.
RefSeqiNP_418737.1. NC_000913.3.
WP_000121005.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZDVNMR-A70-184[»]
1ZDXNMR-A70-170[»]
1ZE3X-ray1.84D46-170[»]
3BWUX-ray1.76D46-170[»]
3OHNX-ray3.01A/B169-708[»]
3RFZX-ray2.80B/E46-878[»]
4J3OX-ray3.80D46-878[»]
ProteinModelPortaliP30130.
SMRiP30130.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262748. 186 interactors.
DIPiDIP-9612N.
IntActiP30130. 7 interactors.
MINTiMINT-1292016.
STRINGi511145.b4317.

Protein family/group databases

TCDBi1.B.11.3.9. the outer membrane fimbrial usher porin (fup) family.

Proteomic databases

PaxDbiP30130.
PRIDEiP30130.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77273; AAC77273; b4317.
BAE78310; BAE78310; BAE78310.
GeneIDi948844.
KEGGiecj:JW5780.
eco:b4317.
PATRICi32124230. VBIEscCol129921_4458.

Organism-specific databases

EchoBASEiEB0307.
EcoGeneiEG10311. fimD.

Phylogenomic databases

eggNOGiENOG4105CW0. Bacteria.
COG3188. LUCA.
HOGENOMiHOG000111414.
InParanoidiP30130.
KOiK07347.
OMAiVPQAFMG.
PhylomeDBiP30130.

Enzyme and pathway databases

BioCyciEcoCyc:EG10311-MONOMER.
ECOL316407:JW5780-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP30130.
PROiP30130.

Family and domain databases

InterProiIPR000015. Fimb_usher.
IPR018030. Fimbrial_membr_usher_CS.
IPR025949. PapC-like_C.
IPR025885. PapC_N.
[Graphical view]
PANTHERiPTHR30451. PTHR30451. 1 hit.
PfamiPF13953. PapC_C. 1 hit.
PF13954. PapC_N. 1 hit.
PF00577. Usher. 1 hit.
[Graphical view]
PROSITEiPS01151. FIMBRIAL_USHER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFIMD_ECOLI
AccessioniPrimary (citable) accession number: P30130
Secondary accession number(s): Q2M5Z6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.