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Protein

Transcription elongation factor GreB

Gene

greB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreB releases sequences of up to 9 nucleotides in length.UniRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

  • DNA-templated transcription, initiation Source: EcoCyc
  • positive regulation of mRNA cleavage Source: EcoCyc
  • regulation of DNA-templated transcription, elongation Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11578-MONOMER.
ECOL316407:JW3369-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription elongation factor GreBUniRule annotation
Alternative name(s):
Transcript cleavage factor GreBUniRule annotation
Gene namesi
Name:greBUniRule annotation
Ordered Locus Names:b3406, JW3369
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11578. greB.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001769221 – 158Transcription elongation factor GreBAdd BLAST158

Proteomic databases

PaxDbiP30128.
PRIDEiP30128.

Interactioni

Protein-protein interaction databases

BioGridi4261264. 21 interactors.
DIPiDIP-9834N.
IntActiP30128. 24 interactors.
MINTiMINT-1255527.
STRINGi511145.b3406.

Structurei

Secondary structure

1158
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 23Combined sources16
Helixi25 – 39Combined sources15
Turni42 – 44Combined sources3
Helixi46 – 71Combined sources26
Beta strandi79 – 81Combined sources3
Beta strandi86 – 88Combined sources3
Beta strandi90 – 94Combined sources5
Beta strandi101 – 105Combined sources5
Beta strandi113 – 115Combined sources3
Helixi123 – 127Combined sources5
Beta strandi136 – 140Combined sources5
Beta strandi145 – 154Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2P4VX-ray2.60A/B/C/D/E/F1-158[»]
ProteinModelPortaliP30128.
SMRiP30128.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP30128.

Family & Domainsi

Sequence similaritiesi

Belongs to the GreA/GreB family. GreB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108RRZ. Bacteria.
COG0782. LUCA.
HOGENOMiHOG000241145.
InParanoidiP30128.
KOiK04760.
OMAiDQIFFGA.
PhylomeDBiP30128.

Family and domain databases

Gene3Di1.10.287.180. 1 hit.
3.10.50.30. 1 hit.
HAMAPiMF_00105. GreA_GreB. 1 hit.
MF_00930. GreB. 1 hit.
InterProiIPR018151. TF_GreA/GreB_CS.
IPR028624. Tscrpt_elong_fac_GreA/B.
IPR001437. Tscrpt_elong_fac_GreA/B_C.
IPR023459. Tscrpt_elong_fac_GreA/B_fam.
IPR022691. Tscrpt_elong_fac_GreA/B_N.
IPR006358. Tscrpt_elong_fac_GreB.
[Graphical view]
PfamiPF01272. GreA_GreB. 1 hit.
PF03449. GreA_GreB_N. 1 hit.
[Graphical view]
PIRSFiPIRSF006092. GreA_GreB. 1 hit.
SUPFAMiSSF46557. SSF46557. 1 hit.
TIGRFAMsiTIGR01461. greB. 1 hit.
PROSITEiPS00829. GREAB_1. 1 hit.
PS00830. GREAB_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30128-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTPLVTREG YEKLKQELNY LWREERPEVT KKVTWAASLG DRSENADYQY
60 70 80 90 100
NKKRLREIDR RVRYLTKCLE NLKIVDYSPQ QEGKVFFGAW VEIENDDGVT
110 120 130 140 150
HRFRIVGYDE IFGRKDYISI DSPMARALLK KEVGDLAVVN TPAGEASWYV

NAIEYVKP
Length:158
Mass (Da):18,527
Last modified:June 1, 1994 - v2
Checksum:i28B46455FABA59CF
GO

Sequence cautioni

The sequence AAA58203 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U81522 Genomic DNA. Translation: AAB39763.1.
U18997 Genomic DNA. Translation: AAA58203.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76431.2.
AP009048 Genomic DNA. Translation: BAE77885.1.
PIRiA65136.
RefSeqiWP_000856737.1. NZ_LN832404.1.
YP_026216.2. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC76431; AAC76431; b3406.
BAE77885; BAE77885; BAE77885.
GeneIDi2847706.
KEGGiecj:JW3369.
eco:b3406.
PATRICi32122248. VBIEscCol129921_3501.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U81522 Genomic DNA. Translation: AAB39763.1.
U18997 Genomic DNA. Translation: AAA58203.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76431.2.
AP009048 Genomic DNA. Translation: BAE77885.1.
PIRiA65136.
RefSeqiWP_000856737.1. NZ_LN832404.1.
YP_026216.2. NC_000913.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2P4VX-ray2.60A/B/C/D/E/F1-158[»]
ProteinModelPortaliP30128.
SMRiP30128.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261264. 21 interactors.
DIPiDIP-9834N.
IntActiP30128. 24 interactors.
MINTiMINT-1255527.
STRINGi511145.b3406.

Proteomic databases

PaxDbiP30128.
PRIDEiP30128.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76431; AAC76431; b3406.
BAE77885; BAE77885; BAE77885.
GeneIDi2847706.
KEGGiecj:JW3369.
eco:b3406.
PATRICi32122248. VBIEscCol129921_3501.

Organism-specific databases

EchoBASEiEB1538.
EcoGeneiEG11578. greB.

Phylogenomic databases

eggNOGiENOG4108RRZ. Bacteria.
COG0782. LUCA.
HOGENOMiHOG000241145.
InParanoidiP30128.
KOiK04760.
OMAiDQIFFGA.
PhylomeDBiP30128.

Enzyme and pathway databases

BioCyciEcoCyc:EG11578-MONOMER.
ECOL316407:JW3369-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP30128.
PROiP30128.

Family and domain databases

Gene3Di1.10.287.180. 1 hit.
3.10.50.30. 1 hit.
HAMAPiMF_00105. GreA_GreB. 1 hit.
MF_00930. GreB. 1 hit.
InterProiIPR018151. TF_GreA/GreB_CS.
IPR028624. Tscrpt_elong_fac_GreA/B.
IPR001437. Tscrpt_elong_fac_GreA/B_C.
IPR023459. Tscrpt_elong_fac_GreA/B_fam.
IPR022691. Tscrpt_elong_fac_GreA/B_N.
IPR006358. Tscrpt_elong_fac_GreB.
[Graphical view]
PfamiPF01272. GreA_GreB. 1 hit.
PF03449. GreA_GreB_N. 1 hit.
[Graphical view]
PIRSFiPIRSF006092. GreA_GreB. 1 hit.
SUPFAMiSSF46557. SSF46557. 1 hit.
TIGRFAMsiTIGR01461. greB. 1 hit.
PROSITEiPS00829. GREAB_1. 1 hit.
PS00830. GREAB_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGREB_ECOLI
AccessioniPrimary (citable) accession number: P30128
Secondary accession number(s): P78114, Q2M771
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: June 1, 1994
Last modified: November 2, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.