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Protein

Transcription elongation factor GreB

Gene

greB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreB releases sequences of up to 9 nucleotides in length.UniRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

  • DNA-templated transcription, initiation Source: EcoCyc
  • positive regulation of mRNA cleavage Source: EcoCyc
  • regulation of DNA-templated transcription, elongation Source: EcoCyc

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:EG11578-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription elongation factor GreBUniRule annotation
Alternative name(s):
Transcript cleavage factor GreBUniRule annotation
Gene namesi
Name:greBUniRule annotation
Ordered Locus Names:b3406, JW3369
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11578 greB

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001769221 – 158Transcription elongation factor GreBAdd BLAST158

Proteomic databases

PaxDbiP30128
PRIDEiP30128

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4261264, 21 interactors
DIPiDIP-9834N
IntActiP30128, 24 interactors
STRINGi316385.ECDH10B_3581

Structurei

Secondary structure

1158
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 23Combined sources16
Helixi25 – 39Combined sources15
Turni42 – 44Combined sources3
Helixi46 – 71Combined sources26
Beta strandi79 – 81Combined sources3
Beta strandi86 – 88Combined sources3
Beta strandi90 – 94Combined sources5
Beta strandi101 – 105Combined sources5
Beta strandi113 – 115Combined sources3
Helixi123 – 127Combined sources5
Beta strandi136 – 140Combined sources5
Beta strandi145 – 154Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2P4VX-ray2.60A/B/C/D/E/F1-158[»]
ProteinModelPortaliP30128
SMRiP30128
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP30128

Family & Domainsi

Sequence similaritiesi

Belongs to the GreA/GreB family. GreB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108RRZ Bacteria
COG0782 LUCA
HOGENOMiHOG000241145
InParanoidiP30128
KOiK04760
OMAiDQIFFGA
PhylomeDBiP30128

Family and domain databases

Gene3Di1.10.287.180, 1 hit
3.10.50.30, 1 hit
HAMAPiMF_00105 GreA_GreB, 1 hit
MF_00930 GreB, 1 hit
InterProiView protein in InterPro
IPR036953 GreA/GreB_C_sf
IPR018151 TF_GreA/GreB_CS
IPR028624 Tscrpt_elong_fac_GreA/B
IPR001437 Tscrpt_elong_fac_GreA/B_C
IPR023459 Tscrpt_elong_fac_GreA/B_fam
IPR022691 Tscrpt_elong_fac_GreA/B_N
IPR036805 Tscrpt_elong_fac_GreA/B_N_sf
IPR006358 Tscrpt_elong_fac_GreB
PANTHERiPTHR30437 PTHR30437, 1 hit
PfamiView protein in Pfam
PF01272 GreA_GreB, 1 hit
PF03449 GreA_GreB_N, 1 hit
PIRSFiPIRSF006092 GreA_GreB, 1 hit
SUPFAMiSSF46557 SSF46557, 1 hit
TIGRFAMsiTIGR01461 greB, 1 hit
PROSITEiView protein in PROSITE
PS00829 GREAB_1, 1 hit
PS00830 GREAB_2, 1 hit

Sequencei

Sequence statusi: Complete.

P30128-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTPLVTREG YEKLKQELNY LWREERPEVT KKVTWAASLG DRSENADYQY
60 70 80 90 100
NKKRLREIDR RVRYLTKCLE NLKIVDYSPQ QEGKVFFGAW VEIENDDGVT
110 120 130 140 150
HRFRIVGYDE IFGRKDYISI DSPMARALLK KEVGDLAVVN TPAGEASWYV

NAIEYVKP
Length:158
Mass (Da):18,527
Last modified:June 1, 1994 - v2
Checksum:i28B46455FABA59CF
GO

Sequence cautioni

The sequence AAA58203 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U81522 Genomic DNA Translation: AAB39763.1
U18997 Genomic DNA Translation: AAA58203.1 Different initiation.
U00096 Genomic DNA Translation: AAC76431.2
AP009048 Genomic DNA Translation: BAE77885.1
PIRiA65136
RefSeqiYP_026216.2, NC_000913.3

Genome annotation databases

EnsemblBacteriaiAAC76431; AAC76431; b3406
BAE77885; BAE77885; BAE77885
GeneIDi2847706
KEGGiecj:JW3369
eco:b3406
PATRICifig|1411691.4.peg.3323

Similar proteinsi

Entry informationi

Entry nameiGREB_ECOLI
AccessioniPrimary (citable) accession number: P30128
Secondary accession number(s): P78114, Q2M771
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: June 1, 1994
Last modified: April 25, 2018
This is version 141 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health