Reviewed, UniProtKB/Swiss-Prot P30115 (GSTA3_MOUSE)

Names and origin

Protein names

Recommended name:
    Glutathione S-transferase A3
    EC=2.5.1.18
Alternative name(s):
    Glutathione S-transferase Yc
    Ya3
    GST class-alpha member 3

Gene names

Name:	Gsta3
Synonyms:	Gstyc

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. This GST has a high catalytic activity for aflatoxin B1-8,9 epoxide.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the GST superfamily. Alpha family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.
Mass spectrometry	Molecular mass is 25271.4±2 Da from positions 2 - 221. Determined by ESI. Ref.5

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Transferase
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 221	220	Glutathione S-transferase A3		PRO_0000185790
Regions
	Domain	3 – 83	81	GST N-terminal
	Domain	85 – 207	123	GST C-terminal
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P30115-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 6F7332488794081F	FASTA	221	25,361
	10 20 30 40 50 60 MAGKPVLHYF DGRGRMEPIR WLLAAAGVEF EEKFLKTRDD LARLRSDGSL MFQQVPMVEI 70 80 90 100 110 120 DGMKLVQTKA ILNYIASKYN LYGKDMKERA IIDMYTEGVA DLEIMILYYP HMPPEEKEAS 130 140 150 160 170 180 LAKIKEQTRN RYFPAFEKVL KSHGQDYLVG NRLSRADIAL VELLYHVEEL DPGVVDNFPL 190 200 210 220 LKALRSRVSN LPTVKKFLQP GSQRKPFDDA KCVESAKKIF S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and heterologous expression of a cDNA encoding a mouse glutathione S-transferase Yc subunit possessing high catalytic activity for aflatoxin B1-8,9-epoxide." Hayes J.D., Judah D.J., Neal G.E., Nguyen T. Biochem. J. 285:173-180(1992) [PubMed: 1637297] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Liver.
[2]	"Complementary DNA cloning, messenger RNA expression, and induction of alpha-class glutathione S-transferases in mouse tissues." Buetler T.M., Eaton D.L. Cancer Res. 52:314-318(1992) [PubMed: 1728405] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Liver.
[4]	"Regulation of mouse glutathione S-transferases by chemoprotectors. Molecular evidence for the existence of three distinct alpha-class glutathione S-transferase subunits, Ya1, Ya2, and Ya3, in mouse liver." McLellan L.I., Kerr L.A., Cronshaw A.D., Hayes J.D. Biochem. J. 276:461-469(1991) [PubMed: 2049074] [Abstract] Cited for: PROTEIN SEQUENCE OF 16-56; 63-73; 94-110 AND 112-142. Tissue: Liver.
[5]	"Purification, mass spectrometric characterization, and covalent modification of murine glutathione S-transferases." Mitchell A.E., Morin D., Lame M.W., Jones A.D. Chem. Res. Toxicol. 8:1054-1062(1995) [PubMed: 8605288] [Abstract] Cited for: CHARACTERIZATION, MASS SPECTROMETRY, ACETYLATION AT ALA-2. Strain: CD-1. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X65021 mRNA. Translation: CAA46155.1. M73483 mRNA. Translation: AAA37751.1. BC009805 mRNA. Translation: AAH09805.1.
IPI	IPI00331241.
PIR	S24322.
RefSeq	NP_001070821.1. NP_034486.2.
UniGene	Mm.394593
3D structure databases
HSSP	HSSP built from PDB template 1K3O based on UniProtKB P08263.
SMR	P30115. Positions 4-220.
ModBase	Search...
Protein-protein interaction databases
IntAct	P30115. 1 interaction.
2-D gel databases
SWISS-2DPAGE	P30115.
Genome annotation databases
Ensembl	ENSMUSG00000025934. Mus musculus. [Contig view]
GeneID	14859.
KEGG	mmu:14859.
Organism-specific databases
MGI	MGI:95856. Gsta3.
Phylogenomic databases
HOGENOM	P30115.
HOVERGEN	P30115.
OMA	P30115. KCVESAK.
Enzyme and pathway databases
BRENDA	2.5.1.18. 244.
Gene expression databases
ArrayExpress	P30115.
Bgee	P30115.
CleanEx	MM_GSTA3.
GermOnline	ENSMUSG00000025934. Mus musculus.
Family and domain databases
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR003080. GST_alpha. IPR004046. GST_C. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:1.20.1050.10. GST_C_like. 1 hit. G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
PANTHER	PTHR11571:SF4. GST_alpha. 1 hit.
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
PRINTS	PR01266. GSTRNSFRASEA.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	287095.
SOURCE	Search...

Entry information

Entry name

GSTA3_MOUSE

Accession

Primary (citable) accession number: P30115

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)