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Protein

Glutathione S-transferase A3

Gene

Gsta3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. This GST has a high catalytic activity for aflatoxin B1-8,9 epoxide.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei9GlutathioneBy similarity1
Binding sitei45GlutathioneBy similarity1

GO - Molecular functioni

GO - Biological processi

  • aflatoxin catabolic process Source: MGI
  • glutathione metabolic process Source: UniProtKB
  • ureteric bud development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase A3 (EC:2.5.1.18)
Alternative name(s):
GST class-alpha member 3
Glutathione S-transferase Ya3
Glutathione S-transferase Yc
Gene namesi
Name:Gsta3
Synonyms:Gstyc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:95856. Gsta3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00001857902 – 221Glutathione S-transferase A3Add BLAST220

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Modified residuei4N6-succinyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP30115.
PaxDbiP30115.
PeptideAtlasiP30115.
PRIDEiP30115.

2D gel databases

SWISS-2DPAGEP30115.

PTM databases

iPTMnetiP30115.
PhosphoSitePlusiP30115.
SwissPalmiP30115.

Expressioni

Gene expression databases

BgeeiENSMUSG00000025934.
CleanExiMM_GSTA3.
ExpressionAtlasiP30115. baseline and differential.
GenevisibleiP30115. MM.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

IntActiP30115. 1 interactor.
MINTiMINT-1864745.
STRINGi10090.ENSMUSP00000027067.

Structurei

3D structure databases

ProteinModelPortaliP30115.
SMRiP30115.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 83GST N-terminalAdd BLAST81
Domaini85 – 207GST C-terminalAdd BLAST123

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni54 – 55Glutathione bindingBy similarity2
Regioni67 – 68Glutathione bindingBy similarity2

Sequence similaritiesi

Belongs to the GST superfamily. Alpha family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiKOG1695. Eukaryota.
ENOG4111VAU. LUCA.
GeneTreeiENSGT00670000097856.
HOGENOMiHOG000115734.
HOVERGENiHBG053749.
InParanoidiP30115.
KOiK00799.
OMAiTRAIANY.
OrthoDBiEOG091G0O3D.
PhylomeDBiP30115.
TreeFamiTF105321.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR003080. GST_alpha.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01266. GSTRNSFRASEA.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30115-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGKPVLHYF DGRGRMEPIR WLLAAAGVEF EEKFLKTRDD LARLRSDGSL
60 70 80 90 100
MFQQVPMVEI DGMKLVQTKA ILNYIASKYN LYGKDMKERA IIDMYTEGVA
110 120 130 140 150
DLEIMILYYP HMPPEEKEAS LAKIKEQTRN RYFPAFEKVL KSHGQDYLVG
160 170 180 190 200
NRLSRADIAL VELLYHVEEL DPGVVDNFPL LKALRSRVSN LPTVKKFLQP
210 220
GSQRKPFDDA KCVESAKKIF S
Length:221
Mass (Da):25,361
Last modified:January 23, 2007 - v2
Checksum:i6F7332488794081F
GO

Mass spectrometryi

Molecular mass is 25271.4±2 Da from positions 2 - 221. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65021 mRNA. Translation: CAA46155.1.
M73483 mRNA. Translation: AAA37751.1.
AK014076 mRNA. Translation: BAB29143.1.
AK149543 mRNA. Translation: BAE28948.1.
CH466536 Genomic DNA. Translation: EDL14389.1.
CH466536 Genomic DNA. Translation: EDL14390.1.
BC147272 mRNA. Translation: AAI47273.1.
BC147273 mRNA. Translation: AAI47274.1.
CCDSiCCDS14847.1.
PIRiS24322.
RefSeqiNP_001070821.1. NM_001077353.2.
NP_034486.2. NM_010356.4.
UniGeneiMm.394593.

Genome annotation databases

EnsembliENSMUST00000027067; ENSMUSP00000027067; ENSMUSG00000025934.
ENSMUST00000121676; ENSMUSP00000113262; ENSMUSG00000025934.
GeneIDi14859.
KEGGimmu:14859.
UCSCiuc007alk.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65021 mRNA. Translation: CAA46155.1.
M73483 mRNA. Translation: AAA37751.1.
AK014076 mRNA. Translation: BAB29143.1.
AK149543 mRNA. Translation: BAE28948.1.
CH466536 Genomic DNA. Translation: EDL14389.1.
CH466536 Genomic DNA. Translation: EDL14390.1.
BC147272 mRNA. Translation: AAI47273.1.
BC147273 mRNA. Translation: AAI47274.1.
CCDSiCCDS14847.1.
PIRiS24322.
RefSeqiNP_001070821.1. NM_001077353.2.
NP_034486.2. NM_010356.4.
UniGeneiMm.394593.

3D structure databases

ProteinModelPortaliP30115.
SMRiP30115.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP30115. 1 interactor.
MINTiMINT-1864745.
STRINGi10090.ENSMUSP00000027067.

PTM databases

iPTMnetiP30115.
PhosphoSitePlusiP30115.
SwissPalmiP30115.

2D gel databases

SWISS-2DPAGEP30115.

Proteomic databases

MaxQBiP30115.
PaxDbiP30115.
PeptideAtlasiP30115.
PRIDEiP30115.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027067; ENSMUSP00000027067; ENSMUSG00000025934.
ENSMUST00000121676; ENSMUSP00000113262; ENSMUSG00000025934.
GeneIDi14859.
KEGGimmu:14859.
UCSCiuc007alk.2. mouse.

Organism-specific databases

CTDi2940.
MGIiMGI:95856. Gsta3.

Phylogenomic databases

eggNOGiKOG1695. Eukaryota.
ENOG4111VAU. LUCA.
GeneTreeiENSGT00670000097856.
HOGENOMiHOG000115734.
HOVERGENiHBG053749.
InParanoidiP30115.
KOiK00799.
OMAiTRAIANY.
OrthoDBiEOG091G0O3D.
PhylomeDBiP30115.
TreeFamiTF105321.

Miscellaneous databases

ChiTaRSiGsta3. mouse.
PROiP30115.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025934.
CleanExiMM_GSTA3.
ExpressionAtlasiP30115. baseline and differential.
GenevisibleiP30115. MM.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR003080. GST_alpha.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01266. GSTRNSFRASEA.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSTA3_MOUSE
AccessioniPrimary (citable) accession number: P30115
Secondary accession number(s): Q544Y6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 151 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.