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P30115

- GSTA3_MOUSE

UniProt

P30115 - GSTA3_MOUSE

Protein

Glutathione S-transferase A3

Gene

Gsta3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. This GST has a high catalytic activity for aflatoxin B1-8,9 epoxide.

    Catalytic activityi

    RX + glutathione = HX + R-S-glutathione.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei9 – 91GlutathioneBy similarity
    Binding sitei45 – 451GlutathioneBy similarity

    GO - Molecular functioni

    1. glutathione transferase activity Source: UniProtKB

    GO - Biological processi

    1. aflatoxin catabolic process Source: MGI
    2. glutathione metabolic process Source: UniProtKB
    3. ureteric bud development Source: UniProtKB

    Keywords - Molecular functioni

    Transferase

    Enzyme and pathway databases

    ReactomeiREACT_215316. Glutathione conjugation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione S-transferase A3 (EC:2.5.1.18)
    Alternative name(s):
    GST class-alpha member 3
    Glutathione S-transferase Ya3
    Glutathione S-transferase Yc
    Gene namesi
    Name:Gsta3
    Synonyms:Gstyc
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:95856. Gsta3.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 221220Glutathione S-transferase A3PRO_0000185790Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine2 Publications
    Modified residuei4 – 41N6-succinyllysine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP30115.
    PaxDbiP30115.
    PRIDEiP30115.

    2D gel databases

    SWISS-2DPAGEP30115.

    PTM databases

    PhosphoSiteiP30115.

    Expressioni

    Gene expression databases

    ArrayExpressiP30115.
    BgeeiP30115.
    CleanExiMM_GSTA3.
    GenevestigatoriP30115.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    IntActiP30115. 1 interaction.
    MINTiMINT-1864745.

    Structurei

    3D structure databases

    ProteinModelPortaliP30115.
    SMRiP30115. Positions 2-206.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 8381GST N-terminalAdd
    BLAST
    Domaini85 – 207123GST C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni54 – 552Glutathione bindingBy similarity
    Regioni67 – 682Glutathione bindingBy similarity

    Sequence similaritiesi

    Belongs to the GST superfamily. Alpha family.Curated
    Contains 1 GST C-terminal domain.Curated
    Contains 1 GST N-terminal domain.Curated

    Phylogenomic databases

    eggNOGiNOG266414.
    GeneTreeiENSGT00670000097856.
    HOGENOMiHOG000115734.
    HOVERGENiHBG053749.
    InParanoidiQ544Y6.
    KOiK00799.
    OMAiKDIPRLM.
    OrthoDBiEOG79CZ0K.
    PhylomeDBiP30115.
    TreeFamiTF105321.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR003080. GST_alpha.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF00043. GST_C. 1 hit.
    PF02798. GST_N. 1 hit.
    [Graphical view]
    PRINTSiPR01266. GSTRNSFRASEA.
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEiPS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P30115-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGKPVLHYF DGRGRMEPIR WLLAAAGVEF EEKFLKTRDD LARLRSDGSL    50
    MFQQVPMVEI DGMKLVQTKA ILNYIASKYN LYGKDMKERA IIDMYTEGVA 100
    DLEIMILYYP HMPPEEKEAS LAKIKEQTRN RYFPAFEKVL KSHGQDYLVG 150
    NRLSRADIAL VELLYHVEEL DPGVVDNFPL LKALRSRVSN LPTVKKFLQP 200
    GSQRKPFDDA KCVESAKKIF S 221
    Length:221
    Mass (Da):25,361
    Last modified:January 23, 2007 - v2
    Checksum:i6F7332488794081F
    GO

    Mass spectrometryi

    Molecular mass is 25271.4±2 Da from positions 2 - 221. Determined by ESI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X65021 mRNA. Translation: CAA46155.1.
    M73483 mRNA. Translation: AAA37751.1.
    AK014076 mRNA. Translation: BAB29143.1.
    AK149543 mRNA. Translation: BAE28948.1.
    CH466536 Genomic DNA. Translation: EDL14389.1.
    CH466536 Genomic DNA. Translation: EDL14390.1.
    BC147272 mRNA. Translation: AAI47273.1.
    BC147273 mRNA. Translation: AAI47274.1.
    CCDSiCCDS14847.1.
    PIRiS24322.
    RefSeqiNP_001070821.1. NM_001077353.2.
    NP_034486.2. NM_010356.4.
    UniGeneiMm.394593.

    Genome annotation databases

    EnsembliENSMUST00000027067; ENSMUSP00000027067; ENSMUSG00000025934.
    ENSMUST00000121676; ENSMUSP00000113262; ENSMUSG00000025934.
    GeneIDi14859.
    KEGGimmu:14859.
    UCSCiuc007alk.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X65021 mRNA. Translation: CAA46155.1 .
    M73483 mRNA. Translation: AAA37751.1 .
    AK014076 mRNA. Translation: BAB29143.1 .
    AK149543 mRNA. Translation: BAE28948.1 .
    CH466536 Genomic DNA. Translation: EDL14389.1 .
    CH466536 Genomic DNA. Translation: EDL14390.1 .
    BC147272 mRNA. Translation: AAI47273.1 .
    BC147273 mRNA. Translation: AAI47274.1 .
    CCDSi CCDS14847.1.
    PIRi S24322.
    RefSeqi NP_001070821.1. NM_001077353.2.
    NP_034486.2. NM_010356.4.
    UniGenei Mm.394593.

    3D structure databases

    ProteinModelPortali P30115.
    SMRi P30115. Positions 2-206.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P30115. 1 interaction.
    MINTi MINT-1864745.

    PTM databases

    PhosphoSitei P30115.

    2D gel databases

    SWISS-2DPAGE P30115.

    Proteomic databases

    MaxQBi P30115.
    PaxDbi P30115.
    PRIDEi P30115.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000027067 ; ENSMUSP00000027067 ; ENSMUSG00000025934 .
    ENSMUST00000121676 ; ENSMUSP00000113262 ; ENSMUSG00000025934 .
    GeneIDi 14859.
    KEGGi mmu:14859.
    UCSCi uc007alk.1. mouse.

    Organism-specific databases

    CTDi 2940.
    MGIi MGI:95856. Gsta3.

    Phylogenomic databases

    eggNOGi NOG266414.
    GeneTreei ENSGT00670000097856.
    HOGENOMi HOG000115734.
    HOVERGENi HBG053749.
    InParanoidi Q544Y6.
    KOi K00799.
    OMAi KDIPRLM.
    OrthoDBi EOG79CZ0K.
    PhylomeDBi P30115.
    TreeFami TF105321.

    Enzyme and pathway databases

    Reactomei REACT_215316. Glutathione conjugation.

    Miscellaneous databases

    NextBioi 287095.
    PROi P30115.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P30115.
    Bgeei P30115.
    CleanExi MM_GSTA3.
    Genevestigatori P30115.

    Family and domain databases

    Gene3Di 1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProi IPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR003080. GST_alpha.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    Pfami PF00043. GST_C. 1 hit.
    PF02798. GST_N. 1 hit.
    [Graphical view ]
    PRINTSi PR01266. GSTRNSFRASEA.
    SUPFAMi SSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEi PS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and heterologous expression of a cDNA encoding a mouse glutathione S-transferase Yc subunit possessing high catalytic activity for aflatoxin B1-8,9-epoxide."
      Hayes J.D., Judah D.J., Neal G.E., Nguyen T.
      Biochem. J. 285:173-180(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Liver.
    2. "Complementary DNA cloning, messenger RNA expression, and induction of alpha-class glutathione S-transferases in mouse tissues."
      Buetler T.M., Eaton D.L.
      Cancer Res. 52:314-318(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Liver.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Head and Liver.
    4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    6. "Regulation of mouse glutathione S-transferases by chemoprotectors. Molecular evidence for the existence of three distinct alpha-class glutathione S-transferase subunits, Ya1, Ya2, and Ya3, in mouse liver."
      McLellan L.I., Kerr L.A., Cronshaw A.D., Hayes J.D.
      Biochem. J. 276:461-469(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 16-56; 63-73; 94-110 AND 112-142.
      Tissue: Liver.
    7. "Purification, mass spectrometric characterization, and covalent modification of murine glutathione S-transferases."
      Mitchell A.E., Morin D., Lame M.W., Jones A.D.
      Chem. Res. Toxicol. 8:1054-1062(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, MASS SPECTROMETRY, ACETYLATION AT ALA-2.
      Strain: CD-1.
      Tissue: Liver.
    8. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-4, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiGSTA3_MOUSE
    AccessioniPrimary (citable) accession number: P30115
    Secondary accession number(s): Q544Y6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 130 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3