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P30115

- GSTA3_MOUSE

UniProt

P30115 - GSTA3_MOUSE

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Protein

Glutathione S-transferase A3

Gene

Gsta3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. This GST has a high catalytic activity for aflatoxin B1-8,9 epoxide.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei9 – 91GlutathioneBy similarity
Binding sitei45 – 451GlutathioneBy similarity

GO - Molecular functioni

  1. glutathione transferase activity Source: UniProtKB

GO - Biological processi

  1. aflatoxin catabolic process Source: MGI
  2. glutathione metabolic process Source: UniProtKB
  3. ureteric bud development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

ReactomeiREACT_215316. Glutathione conjugation.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase A3 (EC:2.5.1.18)
Alternative name(s):
GST class-alpha member 3
Glutathione S-transferase Ya3
Glutathione S-transferase Yc
Gene namesi
Name:Gsta3
Synonyms:Gstyc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:95856. Gsta3.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 221220Glutathione S-transferase A3PRO_0000185790Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine2 Publications
Modified residuei4 – 41N6-succinyllysine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP30115.
PaxDbiP30115.
PRIDEiP30115.

2D gel databases

SWISS-2DPAGEP30115.

PTM databases

PhosphoSiteiP30115.

Expressioni

Gene expression databases

BgeeiP30115.
CleanExiMM_GSTA3.
ExpressionAtlasiP30115. baseline and differential.
GenevestigatoriP30115.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

IntActiP30115. 1 interaction.
MINTiMINT-1864745.

Structurei

3D structure databases

ProteinModelPortaliP30115.
SMRiP30115. Positions 2-206.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 8381GST N-terminalAdd
BLAST
Domaini85 – 207123GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni54 – 552Glutathione bindingBy similarity
Regioni67 – 682Glutathione bindingBy similarity

Sequence similaritiesi

Belongs to the GST superfamily. Alpha family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiNOG266414.
GeneTreeiENSGT00670000097856.
HOGENOMiHOG000115734.
HOVERGENiHBG053749.
InParanoidiP30115.
KOiK00799.
OMAiKDIPRLM.
OrthoDBiEOG79CZ0K.
PhylomeDBiP30115.
TreeFamiTF105321.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR003080. GST_alpha.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01266. GSTRNSFRASEA.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30115-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAGKPVLHYF DGRGRMEPIR WLLAAAGVEF EEKFLKTRDD LARLRSDGSL
60 70 80 90 100
MFQQVPMVEI DGMKLVQTKA ILNYIASKYN LYGKDMKERA IIDMYTEGVA
110 120 130 140 150
DLEIMILYYP HMPPEEKEAS LAKIKEQTRN RYFPAFEKVL KSHGQDYLVG
160 170 180 190 200
NRLSRADIAL VELLYHVEEL DPGVVDNFPL LKALRSRVSN LPTVKKFLQP
210 220
GSQRKPFDDA KCVESAKKIF S
Length:221
Mass (Da):25,361
Last modified:January 23, 2007 - v2
Checksum:i6F7332488794081F
GO

Mass spectrometryi

Molecular mass is 25271.4±2 Da from positions 2 - 221. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65021 mRNA. Translation: CAA46155.1.
M73483 mRNA. Translation: AAA37751.1.
AK014076 mRNA. Translation: BAB29143.1.
AK149543 mRNA. Translation: BAE28948.1.
CH466536 Genomic DNA. Translation: EDL14389.1.
CH466536 Genomic DNA. Translation: EDL14390.1.
BC147272 mRNA. Translation: AAI47273.1.
BC147273 mRNA. Translation: AAI47274.1.
CCDSiCCDS14847.1.
PIRiS24322.
RefSeqiNP_001070821.1. NM_001077353.2.
NP_034486.2. NM_010356.4.
UniGeneiMm.394593.

Genome annotation databases

EnsembliENSMUST00000027067; ENSMUSP00000027067; ENSMUSG00000025934.
ENSMUST00000121676; ENSMUSP00000113262; ENSMUSG00000025934.
GeneIDi14859.
KEGGimmu:14859.
UCSCiuc007alk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65021 mRNA. Translation: CAA46155.1 .
M73483 mRNA. Translation: AAA37751.1 .
AK014076 mRNA. Translation: BAB29143.1 .
AK149543 mRNA. Translation: BAE28948.1 .
CH466536 Genomic DNA. Translation: EDL14389.1 .
CH466536 Genomic DNA. Translation: EDL14390.1 .
BC147272 mRNA. Translation: AAI47273.1 .
BC147273 mRNA. Translation: AAI47274.1 .
CCDSi CCDS14847.1.
PIRi S24322.
RefSeqi NP_001070821.1. NM_001077353.2.
NP_034486.2. NM_010356.4.
UniGenei Mm.394593.

3D structure databases

ProteinModelPortali P30115.
SMRi P30115. Positions 2-206.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P30115. 1 interaction.
MINTi MINT-1864745.

PTM databases

PhosphoSitei P30115.

2D gel databases

SWISS-2DPAGE P30115.

Proteomic databases

MaxQBi P30115.
PaxDbi P30115.
PRIDEi P30115.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000027067 ; ENSMUSP00000027067 ; ENSMUSG00000025934 .
ENSMUST00000121676 ; ENSMUSP00000113262 ; ENSMUSG00000025934 .
GeneIDi 14859.
KEGGi mmu:14859.
UCSCi uc007alk.1. mouse.

Organism-specific databases

CTDi 2940.
MGIi MGI:95856. Gsta3.

Phylogenomic databases

eggNOGi NOG266414.
GeneTreei ENSGT00670000097856.
HOGENOMi HOG000115734.
HOVERGENi HBG053749.
InParanoidi P30115.
KOi K00799.
OMAi KDIPRLM.
OrthoDBi EOG79CZ0K.
PhylomeDBi P30115.
TreeFami TF105321.

Enzyme and pathway databases

Reactomei REACT_215316. Glutathione conjugation.

Miscellaneous databases

ChiTaRSi Gsta3. mouse.
NextBioi 287095.
PROi P30115.
SOURCEi Search...

Gene expression databases

Bgeei P30115.
CleanExi MM_GSTA3.
ExpressionAtlasi P30115. baseline and differential.
Genevestigatori P30115.

Family and domain databases

Gene3Di 1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProi IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR003080. GST_alpha.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view ]
Pfami PF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view ]
PRINTSi PR01266. GSTRNSFRASEA.
SUPFAMi SSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEi PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and heterologous expression of a cDNA encoding a mouse glutathione S-transferase Yc subunit possessing high catalytic activity for aflatoxin B1-8,9-epoxide."
    Hayes J.D., Judah D.J., Neal G.E., Nguyen T.
    Biochem. J. 285:173-180(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Liver.
  2. "Complementary DNA cloning, messenger RNA expression, and induction of alpha-class glutathione S-transferases in mouse tissues."
    Buetler T.M., Eaton D.L.
    Cancer Res. 52:314-318(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Liver.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Head and Liver.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  6. "Regulation of mouse glutathione S-transferases by chemoprotectors. Molecular evidence for the existence of three distinct alpha-class glutathione S-transferase subunits, Ya1, Ya2, and Ya3, in mouse liver."
    McLellan L.I., Kerr L.A., Cronshaw A.D., Hayes J.D.
    Biochem. J. 276:461-469(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 16-56; 63-73; 94-110 AND 112-142.
    Tissue: Liver.
  7. "Purification, mass spectrometric characterization, and covalent modification of murine glutathione S-transferases."
    Mitchell A.E., Morin D., Lame M.W., Jones A.D.
    Chem. Res. Toxicol. 8:1054-1062(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, MASS SPECTROMETRY, ACETYLATION AT ALA-2.
    Strain: CD-1.
    Tissue: Liver.
  8. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-4, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiGSTA3_MOUSE
AccessioniPrimary (citable) accession number: P30115
Secondary accession number(s): Q544Y6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3