Reviewed, UniProtKB/Swiss-Prot P30114 (GST28_SCHHA)

Names and origin

Protein names	Recommended name:     Glutathione S-transferase class-mu 28 kDa isozyme       Short name=GST 28     EC=2.5.1.18 Alternative name(s):     Sh28GST
Organism	Schistosoma haematobium (Blood fluke)
Taxonomic identifier	6185 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Platyhelminthes › Trematoda › Digenea › Strigeidida › Schistosomatoidea › Schistosomatidae › Schistosoma

Protein attributes

Sequence length	211 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the GST superfamily. Mu family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Molecular function	Transferase
Technical term	3D-structure
Gene Ontology (GO)
Molecular function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 211

211

Glutathione S-transferase class-mu 28 kDa isozyme

PRO_0000185813

Regions

Domain

4 – 86

83

GST N-terminal

Domain

88 – 211

124

GST C-terminal

Sites

Active site

10

1

Ref.2

Secondary structure

1

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211

Helix Strand Turn

Details...

Beta strand

5 – 12

8

Helix

18 – 26

9

Beta strand

32 – 35

4

Helix

38 – 40

3

Helix

41 – 44

4

Helix

45 – 47

3

Helix

49 – 51

3

Beta strand

55 – 59

5

Beta strand

65 – 70

6

Helix

71 – 81

11

Helix

89 – 110

22

Helix

119 – 128

10

Helix

131 – 144

14

Beta strand

147 – 152

6

Helix

158 – 173

16

Turn

175 – 180

6

Helix

183 – 195

13

Helix

197 – 206

10

Sequences

	Sequence		Length	Mass (Da)
	P30114-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 954B2B60AA22E2C8	FASTA	211	23,960
	10 20 30 40 50 60 MTGDHIKVIY FNGRGRAESI RMTLVAAGVN YEDERISFQD WPKIKPTIPG GRLPAVKITD 70 80 90 100 110 120 NHGHVKWMLE SLAIARYMAK KHHMMGETDE EYYNVEKLIG QVEDLEHEYH KTLMKPEEEK 130 140 150 160 170 180 QKITKEILNG KVPVLLDIIC ESLKASTGKL AVGDKVTLAD LVLIAVIDHV TDLDKEFLTG 190 200 210 KYPEIHKHRE NLLASSPRLA KYLSDRAATP F

References

[1]	"Inter-species variation of schistosome 28-kDa glutathione S-transferases." Trottein F., Goding G., Sellin B., Gorillot I., Samaio M., Lecocq J.-P., Capron A. Mol. Biochem. Parasitol. 54:63-72(1992) [PubMed: 1518533] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Crystal structure of the 28 kDa glutathione S-transferase from Schistosoma haematobium." Johnson K.A., Angelucci F., Bellelli A., Herve M., Fontaine J., Tsernoglou D., Capron A., Trottein F., Brunori M. Biochemistry 42:10084-10094(2003) [PubMed: 12939136] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS), ACTIVE SITE TYR-10.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M87800 Genomic DNA. Translation: AAA29893.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1OE7	X-ray	1.80	A/B	1-211	[»]
1OE8	X-ray	1.65	A/B	1-211	[»]

ModBase

Search...

Enzyme and pathway databases

BRENDA

2.5.1.18. 277766.

Family and domain databases

InterPro

IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR017933. Glutathione_S_Trfase/Cl_chnl_C.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
IPR012335. Thioredoxin_fold.
[Graphical view]

Gene3D

G3DSA:1.20.1050.10. GST_C_like. 1 hit.
G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.

Pfam

PF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]

PROSITE

PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

GST28_SCHHA

Accession

Primary (citable) accession number: P30114

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	November 24, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)