Reviewed, UniProtKB/Swiss-Prot P30112 (GST26_FASHE)

Names and origin

Protein names	Recommended name:     Glutathione S-transferase class-mu 26 kDa isozyme 51       Short name=GST51     EC=2.5.1.18 Alternative name(s):     Fh51
Organism	Fasciola hepatica (Liver fluke)
Taxonomic identifier	6192 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Platyhelminthes › Trematoda › Digenea › Echinostomida › Echinostomata › Echinostomatoidea › Fasciolidae › Fasciola

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the GST superfamily. Mu family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Transferase
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 218	217	Glutathione S-transferase class-mu 26 kDa isozyme 51		PRO_0000185807
Regions
	Domain	2 – 83	82	GST N-terminal
	Domain	85 – 203	119	GST C-terminal
Sites
	Active site	7	1	By similarity
Experimental info
	Sequence conflict	84	1	T → S Ref.3
	Sequence conflict	135	1	N → D Ref.3
	Sequence conflict	147	1	P → T Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P30112-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: DF8A826E056A2196	FASTA	218	25,373
	10 20 30 40 50 60 MPAKLGYWKI RGLQQPVRLL LEYLGEEYEE HLYGRDDREK WFGDKFNMGL DLPNLPYYID 70 80 90 100 110 120 DKCKLTQSVA IMRYIADKHG MLGTTPEERA RISMIEGAAM DLRMGFVRVC YNPKFEEVKG 130 140 150 160 170 180 DYLKELPTTL KMWSNFLGDR HYLTGSPVSH VDFMVYEALD CIRYLAPQCL EDFPKLKEFK 190 200 210 SRIEDLPKIK AYMESEKFIK WPLNSWIASF GGGDAAPA

References

[1]	"Molecular characterization of cDNA sequences encoding glutathione S-transferases of Fasciola hepatica." Panaccio M., Wilson L.R., Crameri S.L., Wijffels G.L., Spithill T.W. Exp. Parasitol. 74:232-237(1992) [PubMed: 1740183] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Erratum Panaccio M., Wilson L.R., Crameri S.L., Wijffels G.L., Spithill T.W. Exp. Parasitol. 77:385-385(1993) [PubMed: 8224094] [Abstract]
[3]	"Sequence analysis of a Fasciola hepatica glutathione S-transferase cDNA clone." Muro A., Rodriguez-Medina J.R., Hillyer G.V. Am. J. Trop. Med. Hyg. 48:457-463(1993) [PubMed: 7682383] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-218.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M77682 mRNA. Translation: AAA29141.1.
PIR	A48388.
3D structure databases
HSSP	HSSP built from PDB template 1FHE based on UniProtKB P31670.
SMR	P30112. Positions 2-215.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.5.1.18. 39370.
Family and domain databases
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:1.20.1050.10. GST_C_like. 1 hit. G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GST26_FASHE

Accession

Primary (citable) accession number: P30112

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 56 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)