P30110 (GSTF1_WHEAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase 1
EC=2.5.1.18

Alternative name(s):
GST class-phi

Gene names

Name:

GSTA1

Organism

Triticum aestivum (Wheat)

Taxonomic identifier

4565 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Triticum

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Induction	By pathogen infection.
Sequence similarities	Belongs to the GST superfamily. Phi family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Molecular function	Transferase
Gene Ontology (GO)
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 229	229	Glutathione S-transferase 1		PRO_0000185858
Regions
	Domain	2 – 83	82	GST N-terminal
	Domain	93 – 223	131	GST C-terminal
	Region	41 – 42	2	Glutathione binding By similarity
	Region	54 – 55	2	Glutathione binding By similarity
	Region	67 – 68	2	Glutathione binding By similarity
Natural variations
	Natural variant	34	1	M → V.
	Natural variant	171	1	T → S in strain: cv. Fidel.
	Natural variant	195	1	D → E in strain: cv. Fidel.
	Natural variant	226	1	G → R in strain: cv. Fidel.

Sequences

	Sequence		Length	Mass (Da)
	P30110 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: C6A2543D53678570	FASTA	229	25,828
	10 20 30 40 50 60 MSPVKVFGHP MLTNVARVLL FLEEVGAEYE LVPMDFVAGE HKRPQHVQLN PFAKMPGFQD 70 80 90 100 110 120 GDLVLFESRA IAKYILRKYG GTAGLDLLGE NSGIEELAMV DVWTEVEAQQ YYPAISPVVF 130 140 150 160 170 180 ECIIIPFIIP GGGAAPNQTV VDESLERLRG VLGIYEARLE KSRYLAGDSI TFADLNHIPF 190 200 210 220 TFYFMTTPYA KVFDDYPKVK AWWEMLMARP AVQRVCKHMP TEFKLGAQY

References

[1]	"A pathogen-induced wheat gene encodes a protein homologous to glutathione-S-transferases." Dudler R., Hertig C., Rebmann G., Bull J., Mauch F. Mol. Plant Microbe Interact. 4:14-18(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Cheyenne.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X56012 Genomic DNA. Translation: CAA39487.1.
PIR	T06509.
3D structure databases
ProteinModelPortal	P30110.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	P30110.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTF1_WHEAT

Accession

Primary (citable) accession number: P30110

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	April 3, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program