P30109 (GSTF1_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase PARB
EC=2.5.1.18

Alternative name(s):
GST class-phi

Gene names

Name:

PARB

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Developmental stage	Its expression was observed during transition from G0 to the S phase of tobacco mesophyll protoplasts in vitro.
Induction	By auxin.
Sequence similarities	Belongs to the GST superfamily. Phi family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Molecular function	Transferase
Gene Ontology (GO)
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 213	213	Glutathione S-transferase PARB		PRO_0000185856
Regions
	Domain	1 – 82	82	GST N-terminal
	Domain	89 – 213	125	GST C-terminal
	Region	40 – 41	2	Glutathione binding By similarity
	Region	53 – 54	2	Glutathione binding By similarity
	Region	66 – 67	2	Glutathione binding By similarity
Sites
	Binding site	11	1	Glutathione By similarity

Sequences

	Sequence		Length	Mass (Da)
	P30109 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 0AB971171E7CCFDC	FASTA	213	23,967
	10 20 30 40 50 60 MAIKVHGSPM STATMRVAAC LIEKELDFEF VPVDMASGEH KKHPYLSLNP FGQVPAFEDG 70 80 90 100 110 120 DLKLFESRAI TQYIAHVYAD NGYQLILQDP KKMPSMSVWM EVEGQKFEPP ATKLTWELGI 130 140 150 160 170 180 KPIIGMTTDD AAVKESEAQL SKVLDIYETQ LAESKYLGGD SFTLVDLHHI PNIYYLMSSK 190 200 210 VKEVFDSRPR VSAWCADILA RPAWVKGLEK LQK

References

[1]	"parB: an auxin-regulated gene encoding glutathione S-transferase." Takahashi Y., Nagata T. Proc. Natl. Acad. Sci. U.S.A. 89:56-59(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Xanthi NC. Tissue: Leaf mesophyll.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D10524 mRNA. Translation: BAA01394.1.
PIR	A41789.
3D structure databases
ProteinModelPortal	P30109.
SMR	P30109. Positions 2-206.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTF1_TOBAC

Accession

Primary (citable) accession number: P30109

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	April 3, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program