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P30100

- C11B3_RAT

UniProt

P30100 - C11B3_RAT

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Protein

Cytochrome P450 11B3, mitochondrial

Gene

Cyp11b3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Converts 11-deoxycorticosterone into corticosterone, 18-hydroxycorticosterone, and aldosterone. Also can catalyze the conversion of 11-deoxycortisol to cortisol, 18-hydroxycortisol and cortisone.

Catalytic activityi

A steroid + reduced adrenodoxin + O2 = an 11-beta-hydroxysteroid + oxidized adrenodoxin + H2O.
Corticosterone + reduced adrenodoxin + O2 = 18-hydroxycorticosterone + oxidized adrenodoxin + H2O.

Cofactori

Heme group.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi447 – 4471Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. corticosterone 18-monooxygenase activity Source: RGD
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro
  4. steroid 11-beta-monooxygenase activity Source: RGD

GO - Biological processi

  1. adrenal gland development Source: RGD
  2. response to peptide hormone Source: RGD
  3. steroid biosynthetic process Source: UniProtKB-KW
  4. steroid metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Steroidogenesis

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 11B3, mitochondrial
Alternative name(s):
Aldosterone synthase
CYPXIB3
Cytochrome P450-Aldo-2
Steroid 11-beta-hydroxylase (EC:1.14.15.4, EC:1.14.15.5)
Gene namesi
Name:Cyp11b3
Synonyms:Cyp11b-3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi727886. Cyp11b3.

Subcellular locationi

GO - Cellular componenti

  1. membrane Source: UniProtKB-KW
  2. mitochondrion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2424Mitochondrion1 PublicationAdd
BLAST
Chaini25 – 500476Cytochrome P450 11B3, mitochondrialPRO_0000003605Add
BLAST

Expressioni

Tissue specificityi

Adrenal cortex.

Gene expression databases

GenevestigatoriP30100.

Structurei

3D structure databases

ProteinModelPortaliP30100.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOVERGENiHBG051098.
InParanoidiP30100.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002399. Cyt_P450_mitochondrial.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00408. MITP450.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30100-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALRVTADVW LARPWQCLHR TRALGTTATL APKTLKPFEA IPQYSRNKWL
60 70 80 90 100
KMIQILREQG QENLHLEMHQ AFQELGPIFR HSAGGAQIVS VMLPEDAEKL
110 120 130 140 150
HQVESILPRR MHLEPWVAHR ELRGLRRGVF LLNGAEWRFN RLKLNPNVLS
160 170 180 190 200
PKAVQNFVPM VDEVARDFLE ALKKKVRQNA RGSLTMDVQQ SLFNYTIEAS
210 220 230 240 250
NFALFGERLG LLGHDLNPGS LKFIHALHSM FKSTTQLLFL PRSLTRWTST
260 270 280 290 300
QVWKEHFDAW DVISEYANRC IWKVHQELRL GSSQTYSGIV AALITQGALP
310 320 330 340 350
LDAIKANSMK LTAGSVDTTA IPLVMTLFEL ARNPDVQQAL RQETLAAEAS
360 370 380 390 400
IAANPQKAMS DLPLLRAALK ETLRLYPVGG FLERILNSDL VLQNYHVPAG
410 420 430 440 450
TLVLLYLYSM GRNPAVFPRP ERYMPQRWLE RKRSFQHLAF GFGVRQCLGR
460 470 480 490 500
RLAEVEMLLL LHHMLKTFQV ETLRQEDVQM AYRFVLMPSS SPVLTFRPIS
Length:500
Mass (Da):57,122
Last modified:April 1, 1993 - v1
Checksum:i040F6ECCA84CDEAD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti310 – 3101K → E in CAA36978. (PubMed:2129527)Curated
Sequence conflicti310 – 3101K → E in BAA03172. (PubMed:8473352)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D00568 mRNA. Translation: BAA00445.1.
X52766 mRNA. Translation: CAA36978.1.
D14097 Genomic DNA. Translation: BAA03172.1.
PIRiJX0252.
UniGeneiRn.144549.

Genome annotation databases

UCSCiRGD:727886. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D00568 mRNA. Translation: BAA00445.1 .
X52766 mRNA. Translation: CAA36978.1 .
D14097 Genomic DNA. Translation: BAA03172.1 .
PIRi JX0252.
UniGenei Rn.144549.

3D structure databases

ProteinModelPortali P30100.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:727886. rat.

Organism-specific databases

RGDi 727886. Cyp11b3.

Phylogenomic databases

HOVERGENi HBG051098.
InParanoidi P30100.

Gene expression databases

Genevestigatori P30100.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002399. Cyt_P450_mitochondrial.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00408. MITP450.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and expression of cDNAS encoding rat aldosterone synthase: variants of cytochrome P-450(11 beta)."
    Matsukawa N., Nonaka Y., Ying Z., Higaki J., Ogihara T., Okamoto M.
    Biochem. Biophys. Res. Commun. 169:245-252(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Adrenal gland.
  2. "Molecular biology of rat steroid 11 beta-hydroxylase [P450(11 beta)] and aldosterone synthase [P450(11 beta, aldo)]."
    Okamoto M., Nonaka Y.
    J. Steroid Biochem. Mol. Biol. 41:415-419(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  3. "Molecular cloning of a cDNA encoding aldosterone synthase cytochrome P-450 in rat adrenal cortex."
    Imai M., Shimada H., Okada Y., Matsushima-Hibiya Y., Ogishima T., Ishimura Y.
    FEBS Lett. 263:299-302(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Adrenal gland.
  4. "Isolation and characterization of rat CYP11B genes involved in late steps of mineralo- and glucocorticoid syntheses."
    Mukai K., Imai M., Shimada H., Ishimura Y.
    J. Biol. Chem. 268:9130-9137(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "Three forms of rat CYP11B genes: 11 beta-hydroxylase gene, aldosterone synthase gene, and a novel gene."
    Nomura M., Morohashi K., Kirita S., Nonaka Y., Okamoto M., Nawata H., Omura T.
    J. Biochem. 113:144-152(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  6. "Isolation of aldosterone synthase cytochrome P-450 from zona glomerulosa mitochondria of rat adrenal cortex."
    Ogishima T., Mitani F., Ishimura Y.
    J. Biol. Chem. 264:10935-10938(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 25-44.
    Strain: Sprague-Dawley.
    Tissue: Adrenal gland.

Entry informationi

Entry nameiC11B3_RAT
AccessioniPrimary (citable) accession number: P30100
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: October 29, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3