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P30100 (C11B3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 11B3, mitochondrial
Alternative name(s):
Aldosterone synthase
CYPXIB3
Cytochrome P450-Aldo-2
Steroid 11-beta-hydroxylase
EC=1.14.15.4
EC=1.14.15.5
Gene names
Name:Cyp11b3
Synonyms:Cyp11b-3
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length500 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Converts 11-deoxycorticosterone into corticosterone, 18-hydroxycorticosterone, and aldosterone. Also can catalyze the conversion of 11-deoxycortisol to cortisol, 18-hydroxycortisol and cortisone.

Catalytic activity

A steroid + reduced adrenodoxin + O2 = an 11-beta-hydroxysteroid + oxidized adrenodoxin + H2O.

Corticosterone + reduced adrenodoxin + O2 = 18-hydroxycorticosterone + oxidized adrenodoxin + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Mitochondrion membrane.

Tissue specificity

Adrenal cortex.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2424Mitochondrion Ref.6
Chain25 – 500476Cytochrome P450 11B3, mitochondrial
PRO_0000003605

Sites

Metal binding4471Iron (heme axial ligand) By similarity

Experimental info

Sequence conflict3101K → E in CAA36978. Ref.3
Sequence conflict3101K → E in BAA03172. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P30100 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 040F6ECCA84CDEAD

FASTA50057,122
        10         20         30         40         50         60 
MALRVTADVW LARPWQCLHR TRALGTTATL APKTLKPFEA IPQYSRNKWL KMIQILREQG 

        70         80         90        100        110        120 
QENLHLEMHQ AFQELGPIFR HSAGGAQIVS VMLPEDAEKL HQVESILPRR MHLEPWVAHR 

       130        140        150        160        170        180 
ELRGLRRGVF LLNGAEWRFN RLKLNPNVLS PKAVQNFVPM VDEVARDFLE ALKKKVRQNA 

       190        200        210        220        230        240 
RGSLTMDVQQ SLFNYTIEAS NFALFGERLG LLGHDLNPGS LKFIHALHSM FKSTTQLLFL 

       250        260        270        280        290        300 
PRSLTRWTST QVWKEHFDAW DVISEYANRC IWKVHQELRL GSSQTYSGIV AALITQGALP 

       310        320        330        340        350        360 
LDAIKANSMK LTAGSVDTTA IPLVMTLFEL ARNPDVQQAL RQETLAAEAS IAANPQKAMS 

       370        380        390        400        410        420 
DLPLLRAALK ETLRLYPVGG FLERILNSDL VLQNYHVPAG TLVLLYLYSM GRNPAVFPRP 

       430        440        450        460        470        480 
ERYMPQRWLE RKRSFQHLAF GFGVRQCLGR RLAEVEMLLL LHHMLKTFQV ETLRQEDVQM 

       490        500 
AYRFVLMPSS SPVLTFRPIS 

« Hide

References

[1]"Molecular cloning and expression of cDNAS encoding rat aldosterone synthase: variants of cytochrome P-450(11 beta)."
Matsukawa N., Nonaka Y., Ying Z., Higaki J., Ogihara T., Okamoto M.
Biochem. Biophys. Res. Commun. 169:245-252(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Adrenal gland.
[2]"Molecular biology of rat steroid 11 beta-hydroxylase [P450(11 beta)] and aldosterone synthase [P450(11 beta, aldo)]."
Okamoto M., Nonaka Y.
J. Steroid Biochem. Mol. Biol. 41:415-419(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[3]"Molecular cloning of a cDNA encoding aldosterone synthase cytochrome P-450 in rat adrenal cortex."
Imai M., Shimada H., Okada Y., Matsushima-Hibiya Y., Ogishima T., Ishimura Y.
FEBS Lett. 263:299-302(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Adrenal gland.
[4]"Isolation and characterization of rat CYP11B genes involved in late steps of mineralo- and glucocorticoid syntheses."
Mukai K., Imai M., Shimada H., Ishimura Y.
J. Biol. Chem. 268:9130-9137(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"Three forms of rat CYP11B genes: 11 beta-hydroxylase gene, aldosterone synthase gene, and a novel gene."
Nomura M., Morohashi K., Kirita S., Nonaka Y., Okamoto M., Nawata H., Omura T.
J. Biochem. 113:144-152(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[6]"Isolation of aldosterone synthase cytochrome P-450 from zona glomerulosa mitochondria of rat adrenal cortex."
Ogishima T., Mitani F., Ishimura Y.
J. Biol. Chem. 264:10935-10938(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-44.
Strain: Sprague-Dawley.
Tissue: Adrenal gland.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D00568 mRNA. Translation: BAA00445.1.
X52766 mRNA. Translation: CAA36978.1.
D14097 Genomic DNA. Translation: BAA03172.1.
PIRJX0252.
UniGeneRn.144549.

3D structure databases

ProteinModelPortalP30100.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:727886. rat.

Organism-specific databases

RGD727886. Cyp11b3.

Phylogenomic databases

HOVERGENHBG051098.
InParanoidP30100.

Gene expression databases

GenevestigatorP30100.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002399. Cyt_P450_mitochondrial.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00408. MITP450.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameC11B3_RAT
AccessionPrimary (citable) accession number: P30100
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: February 19, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families