Reviewed, UniProtKB/Swiss-Prot P30100 (C11B3_RAT)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 11B3, mitochondrial
Alternative name(s):
    CYPXIB3
    P450-Aldo-2
    Steroid 11-beta-hydroxylase
    EC=1.14.15.4
    EC=1.14.15.5
    Aldosterone synthase

Gene names

Name:	Cyp11b3
Synonyms:	Cyp11b-3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Converts 11-deoxycorticosterone into corticosterone, 18-hydroxycorticosterone, and aldosterone. Also can catalyze the conversion of 11-deoxycortisol to cortisol, 18-hydroxycortisol and cortisone.
Catalytic activity	A steroid + reduced adrenal ferredoxin + O2 = an 11-beta-hydroxysteroid + oxidized adrenal ferredoxin + H2O. Corticosterone + reduced adrenal ferredoxin + O2 = 18-hydroxycorticosterone + oxidized adrenal ferredoxin + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Mitochondrion membrane.
Tissue specificity	Adrenal cortex.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Steroidogenesis
Cellular component	Membrane Mitochondrion
Domain	Transit peptide
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	C21-steroid hormone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	corticosterone 18-monooxygenase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro steroid 11-beta-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 24	24	Mitochondrion Ref.6
	Chain	25 – 500	476	Cytochrome P450 11B3, mitochondrial		PRO_0000003605
Sites
	Metal binding	447	1	Iron (heme axial ligand) By similarity
Experimental info
	Sequence conflict	310	1	K → E Ref.3
	Sequence conflict	310	1	K → E Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P30100-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 040F6ECCA84CDEAD	FASTA	500	57,122
	10 20 30 40 50 60 MALRVTADVW LARPWQCLHR TRALGTTATL APKTLKPFEA IPQYSRNKWL KMIQILREQG 70 80 90 100 110 120 QENLHLEMHQ AFQELGPIFR HSAGGAQIVS VMLPEDAEKL HQVESILPRR MHLEPWVAHR 130 140 150 160 170 180 ELRGLRRGVF LLNGAEWRFN RLKLNPNVLS PKAVQNFVPM VDEVARDFLE ALKKKVRQNA 190 200 210 220 230 240 RGSLTMDVQQ SLFNYTIEAS NFALFGERLG LLGHDLNPGS LKFIHALHSM FKSTTQLLFL 250 260 270 280 290 300 PRSLTRWTST QVWKEHFDAW DVISEYANRC IWKVHQELRL GSSQTYSGIV AALITQGALP 310 320 330 340 350 360 LDAIKANSMK LTAGSVDTTA IPLVMTLFEL ARNPDVQQAL RQETLAAEAS IAANPQKAMS 370 380 390 400 410 420 DLPLLRAALK ETLRLYPVGG FLERILNSDL VLQNYHVPAG TLVLLYLYSM GRNPAVFPRP 430 440 450 460 470 480 ERYMPQRWLE RKRSFQHLAF GFGVRQCLGR RLAEVEMLLL LHHMLKTFQV ETLRQEDVQM 490 500 AYRFVLMPSS SPVLTFRPIS

References

[1]	"Molecular cloning and expression of cDNAS encoding rat aldosterone synthase: variants of cytochrome P-450(11 beta)." Matsukawa N., Nonaka Y., Ying Z., Higaki J., Ogihara T., Okamoto M. Biochem. Biophys. Res. Commun. 169:245-252(1990) [PubMed: 2350348] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Adrenal gland.
[2]	"Molecular biology of rat steroid 11 beta-hydroxylase [P450(11 beta)] and aldosterone synthase [P450(11 beta, aldo)]." Okamoto M., Nonaka Y. J. Steroid Biochem. Mol. Biol. 41:415-419(1992) [PubMed: 1562515] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[3]	"Molecular cloning of a cDNA encoding aldosterone synthase cytochrome P-450 in rat adrenal cortex." Imai M., Shimada H., Okada Y., Matsushima-Hibiya Y., Ogishima T., Ishimura Y. FEBS Lett. 263:299-302(1990) [PubMed: 2129527] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Adrenal gland.
[4]	"Isolation and characterization of rat CYP11B genes involved in late steps of mineralo- and glucocorticoid syntheses." Mukai K., Imai M., Shimada H., Ishimura Y. J. Biol. Chem. 268:9130-9137(1993) [PubMed: 8473352] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[5]	"Three forms of rat CYP11B genes: 11 beta-hydroxylase gene, aldosterone synthase gene, and a novel gene." Nomura M., Morohashi K., Kirita S., Nonaka Y., Okamoto M., Nawata H., Omura T. J. Biochem. 113:144-152(1993) [PubMed: 8468320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[6]	"Isolation of aldosterone synthase cytochrome P-450 from zona glomerulosa mitochondria of rat adrenal cortex." Ogishima T., Mitani F., Ishimura Y. J. Biol. Chem. 264:10935-10938(1989) [PubMed: 2738055] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-44. Strain: Sprague-Dawley. Tissue: Adrenal gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D00568 mRNA. Translation: BAA00445.1. X52766 mRNA. Translation: CAA36978.1. D14097 Genomic DNA. Translation: BAA03172.1.
IPI	IPI00205747.
PIR	JX0252.
UniGene	Rn.144549
3D structure databases
HSSP	HSSP built from PDB template 1SCC based on UniProtKB P00189.
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000030111. Rattus norvegicus. [Contig view]
KEGG	rno:24294.
Organism-specific databases
RGD	727886. Cyp11b3.
Phylogenomic databases
HOVERGEN	P30100.
Enzyme and pathway databases
BRENDA	1.14.15.4. 248. 1.14.15.5. 248.
Gene expression databases
ArrayExpress	P30100.
GermOnline	ENSRNOG00000030111. Rattus norvegicus.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002399. Cyt_P450_mit_N. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00408. MITP450. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	602896.

Entry information

Entry name

C11B3_RAT

Accession

Primary (citable) accession number: P30100

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	May 26, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)