Cytochrome P450 11B3, mitochondrial precursor

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P30100 (C11B3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 100. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cytochrome P450 11B3, mitochondrial

Alternative name(s):
Aldosterone synthase
CYPXIB3
Cytochrome P450-Aldo-2
Steroid 11-beta-hydroxylase
EC=1.14.15.4
EC=1.14.15.5

Gene names

Name:	Cyp11b3
Synonyms:	Cyp11b-3

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Converts 11-deoxycorticosterone into corticosterone, 18-hydroxycorticosterone, and aldosterone. Also can catalyze the conversion of 11-deoxycortisol to cortisol, 18-hydroxycortisol and cortisone.
Catalytic activity	A steroid + reduced adrenal ferredoxin + O₂ = an 11-beta-hydroxysteroid + oxidized adrenal ferredoxin + H₂O. Corticosterone + reduced adrenal ferredoxin + O₂ = 18-hydroxycorticosterone + oxidized adrenal ferredoxin + H₂O.
Cofactor	Heme group By similarity.
Subcellular location	Mitochondrion membrane.
Tissue specificity	Adrenal cortex.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Steroidogenesis
Cellular component	Membrane Mitochondrion
Domain	Transit peptide
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	adrenal gland development Inferred from expression pattern PubMed 7829497. Source: RGD response to peptide hormone stimulus Inferred from expression pattern PubMed 7829497. Source: RGD steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW steroid metabolic process Inferred from direct assay PubMed 7829497. Source: RGD
Cellular_component	mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	corticosterone 18-monooxygenase activity Inferred from direct assay PubMed 7829497. Source: RGD electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro steroid 11-beta-monooxygenase activity Inferred from direct assay PubMed 7829497. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 24

Mitochondrion Ref.6

Chain

25 – 500

476

Cytochrome P450 11B3, mitochondrial

PRO_0000003605

Sites

Metal binding

447

Iron (heme axial ligand) By similarity

Experimental info

Sequence conflict

310

K → E in CAA36978. Ref.3

Sequence conflict

310

K → E in BAA03172. Ref.4

Sequences

Sequence

Length

Mass (Da)

Tools

P30100 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 040F6ECCA84CDEAD
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FASTA

500

57,122

        10         20         30         40         50         60 
MALRVTADVW LARPWQCLHR TRALGTTATL APKTLKPFEA IPQYSRNKWL KMIQILREQG 

        70         80         90        100        110        120 
QENLHLEMHQ AFQELGPIFR HSAGGAQIVS VMLPEDAEKL HQVESILPRR MHLEPWVAHR 

       130        140        150        160        170        180 
ELRGLRRGVF LLNGAEWRFN RLKLNPNVLS PKAVQNFVPM VDEVARDFLE ALKKKVRQNA 

       190        200        210        220        230        240 
RGSLTMDVQQ SLFNYTIEAS NFALFGERLG LLGHDLNPGS LKFIHALHSM FKSTTQLLFL 

       250        260        270        280        290        300 
PRSLTRWTST QVWKEHFDAW DVISEYANRC IWKVHQELRL GSSQTYSGIV AALITQGALP 

       310        320        330        340        350        360 
LDAIKANSMK LTAGSVDTTA IPLVMTLFEL ARNPDVQQAL RQETLAAEAS IAANPQKAMS 

       370        380        390        400        410        420 
DLPLLRAALK ETLRLYPVGG FLERILNSDL VLQNYHVPAG TLVLLYLYSM GRNPAVFPRP 

       430        440        450        460        470        480 
ERYMPQRWLE RKRSFQHLAF GFGVRQCLGR RLAEVEMLLL LHHMLKTFQV ETLRQEDVQM 

       490        500 
AYRFVLMPSS SPVLTFRPIS

« Hide

References

[1]	"Molecular cloning and expression of cDNAS encoding rat aldosterone synthase: variants of cytochrome P-450(11 beta)." Matsukawa N., Nonaka Y., Ying Z., Higaki J., Ogihara T., Okamoto M. Biochem. Biophys. Res. Commun. 169:245-252(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Adrenal gland.
[2]	"Molecular biology of rat steroid 11 beta-hydroxylase [P450(11 beta)] and aldosterone synthase [P450(11 beta, aldo)]." Okamoto M., Nonaka Y. J. Steroid Biochem. Mol. Biol. 41:415-419(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[3]	"Molecular cloning of a cDNA encoding aldosterone synthase cytochrome P-450 in rat adrenal cortex." Imai M., Shimada H., Okada Y., Matsushima-Hibiya Y., Ogishima T., Ishimura Y. FEBS Lett. 263:299-302(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Adrenal gland.
[4]	"Isolation and characterization of rat CYP11B genes involved in late steps of mineralo- and glucocorticoid syntheses." Mukai K., Imai M., Shimada H., Ishimura Y. J. Biol. Chem. 268:9130-9137(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"Three forms of rat CYP11B genes: 11 beta-hydroxylase gene, aldosterone synthase gene, and a novel gene." Nomura M., Morohashi K., Kirita S., Nonaka Y., Okamoto M., Nawata H., Omura T. J. Biochem. 113:144-152(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[6]	"Isolation of aldosterone synthase cytochrome P-450 from zona glomerulosa mitochondria of rat adrenal cortex." Ogishima T., Mitani F., Ishimura Y. J. Biol. Chem. 264:10935-10938(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-44. Strain: Sprague-Dawley. Tissue: Adrenal gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D00568 mRNA. Translation: BAA00445.1. X52766 mRNA. Translation: CAA36978.1. D14097 Genomic DNA. Translation: BAA03172.1.
IPI	IPI00205747.
PIR	JX0252.
UniGene	Rn.144549.
3D structure databases
ProteinModelPortal	P30100.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
UCSC	RGD:727886. rat.
Organism-specific databases
RGD	727886. Cyp11b3.
Phylogenomic databases
HOVERGEN	HBG051098.
InParanoid	P30100.
Gene expression databases
ArrayExpress	P30100.
Genevestigator	P30100.
GermOnline	ENSRNOG00000030111. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002399. Cyt_P450_mitochondrial. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00408. MITP450. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C11B3_RAT

Accession

Primary (citable) accession number: P30100

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	April 3, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents