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Protein

Cytochrome P450 11B3, mitochondrial

Gene

Cyp11b3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Converts 11-deoxycorticosterone into corticosterone, 18-hydroxycorticosterone, and aldosterone. Also can catalyze the conversion of 11-deoxycortisol to cortisol, 18-hydroxycortisol and cortisone.

Catalytic activityi

A steroid + 2 reduced adrenodoxin + O2 + 2 H+ = an 11-beta- hydroxysteroid + 2 oxidized adrenodoxin + H2O.By similarity
Corticosterone + 2 reduced adrenodoxin + O2 + 2 H+ = 18-hydroxycorticosterone + 2 oxidized adrenodoxin + H2O.By similarity

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi447Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Steroidogenesis

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 11B3, mitochondrial
Alternative name(s):
Aldosterone synthase
CYPXIB3
Cytochrome P450-Aldo-2
Steroid 11-beta-hydroxylase (EC:1.14.15.4, EC:1.14.15.5)
Gene namesi
Name:Cyp11b3
Synonyms:Cyp11b-3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi727886. Cyp11b3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 24Mitochondrion1 PublicationAdd BLAST24
ChainiPRO_000000360525 – 500Cytochrome P450 11B3, mitochondrialAdd BLAST476

Proteomic databases

PaxDbiP30100.

Expressioni

Tissue specificityi

Adrenal cortex.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000049722.

Structurei

3D structure databases

ProteinModelPortaliP30100.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0159. Eukaryota.
COG2124. LUCA.
HOVERGENiHBG051098.
InParanoidiP30100.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002399. Cyt_P450_mitochondrial.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00408. MITP450.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30100-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALRVTADVW LARPWQCLHR TRALGTTATL APKTLKPFEA IPQYSRNKWL
60 70 80 90 100
KMIQILREQG QENLHLEMHQ AFQELGPIFR HSAGGAQIVS VMLPEDAEKL
110 120 130 140 150
HQVESILPRR MHLEPWVAHR ELRGLRRGVF LLNGAEWRFN RLKLNPNVLS
160 170 180 190 200
PKAVQNFVPM VDEVARDFLE ALKKKVRQNA RGSLTMDVQQ SLFNYTIEAS
210 220 230 240 250
NFALFGERLG LLGHDLNPGS LKFIHALHSM FKSTTQLLFL PRSLTRWTST
260 270 280 290 300
QVWKEHFDAW DVISEYANRC IWKVHQELRL GSSQTYSGIV AALITQGALP
310 320 330 340 350
LDAIKANSMK LTAGSVDTTA IPLVMTLFEL ARNPDVQQAL RQETLAAEAS
360 370 380 390 400
IAANPQKAMS DLPLLRAALK ETLRLYPVGG FLERILNSDL VLQNYHVPAG
410 420 430 440 450
TLVLLYLYSM GRNPAVFPRP ERYMPQRWLE RKRSFQHLAF GFGVRQCLGR
460 470 480 490 500
RLAEVEMLLL LHHMLKTFQV ETLRQEDVQM AYRFVLMPSS SPVLTFRPIS
Length:500
Mass (Da):57,122
Last modified:April 1, 1993 - v1
Checksum:i040F6ECCA84CDEAD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti310K → E in CAA36978 (PubMed:2129527).Curated1
Sequence conflicti310K → E in BAA03172 (PubMed:8473352).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00568 mRNA. Translation: BAA00445.1.
X52766 mRNA. Translation: CAA36978.1.
D14097 Genomic DNA. Translation: BAA03172.1.
PIRiJX0252.
UniGeneiRn.144549.

Genome annotation databases

UCSCiRGD:727886. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00568 mRNA. Translation: BAA00445.1.
X52766 mRNA. Translation: CAA36978.1.
D14097 Genomic DNA. Translation: BAA03172.1.
PIRiJX0252.
UniGeneiRn.144549.

3D structure databases

ProteinModelPortaliP30100.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000049722.

Proteomic databases

PaxDbiP30100.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:727886. rat.

Organism-specific databases

RGDi727886. Cyp11b3.

Phylogenomic databases

eggNOGiKOG0159. Eukaryota.
COG2124. LUCA.
HOVERGENiHBG051098.
InParanoidiP30100.

Miscellaneous databases

PROiP30100.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002399. Cyt_P450_mitochondrial.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00408. MITP450.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiC11B3_RAT
AccessioniPrimary (citable) accession number: P30100
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: July 6, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.