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Reviewed, UniProtKB/Swiss-Prot P30100 (C11B3_RAT)

Last modified May 26, 2009. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Cytochrome P450 11B3, mitochondrial
Alternative name(s):
    CYPXIB3
    P450-Aldo-2
    Steroid 11-beta-hydroxylase
    EC=1.14.15.4
    EC=1.14.15.5
    Aldosterone synthase
Gene names
Name: Cyp11b3
Synonyms: Cyp11b-3
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length500 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Converts 11-deoxycorticosterone into corticosterone, 18-hydroxycorticosterone, and aldosterone. Also can catalyze the conversion of 11-deoxycortisol to cortisol, 18-hydroxycortisol and cortisone.

Catalytic activity

A steroid + reduced adrenal ferredoxin + O2 = an 11-beta-hydroxysteroid + oxidized adrenal ferredoxin + H2O.

Corticosterone + reduced adrenal ferredoxin + O2 = 18-hydroxycorticosterone + oxidized adrenal ferredoxin + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Mitochondrion membrane.

Tissue specificity

Adrenal cortex.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2424Mitochondrion Ref.6
Chain25 – 500476Cytochrome P450 11B3, mitochondrial
PRO_0000003605

Sites

Metal binding4471Iron (heme axial ligand) By similarity

Experimental info

Sequence conflict3101K → E Ref.3
Sequence conflict3101K → E Ref.4

Sequences

Sequence LengthMass (Da)Tools
P30100-1 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 040F6ECCA84CDEAD

FASTA50057,122
        10         20         30         40         50         60 
MALRVTADVW LARPWQCLHR TRALGTTATL APKTLKPFEA IPQYSRNKWL KMIQILREQG 

        70         80         90        100        110        120 
QENLHLEMHQ AFQELGPIFR HSAGGAQIVS VMLPEDAEKL HQVESILPRR MHLEPWVAHR 

       130        140        150        160        170        180 
ELRGLRRGVF LLNGAEWRFN RLKLNPNVLS PKAVQNFVPM VDEVARDFLE ALKKKVRQNA 

       190        200        210        220        230        240 
RGSLTMDVQQ SLFNYTIEAS NFALFGERLG LLGHDLNPGS LKFIHALHSM FKSTTQLLFL 

       250        260        270        280        290        300 
PRSLTRWTST QVWKEHFDAW DVISEYANRC IWKVHQELRL GSSQTYSGIV AALITQGALP 

       310        320        330        340        350        360 
LDAIKANSMK LTAGSVDTTA IPLVMTLFEL ARNPDVQQAL RQETLAAEAS IAANPQKAMS 

       370        380        390        400        410        420 
DLPLLRAALK ETLRLYPVGG FLERILNSDL VLQNYHVPAG TLVLLYLYSM GRNPAVFPRP 

       430        440        450        460        470        480 
ERYMPQRWLE RKRSFQHLAF GFGVRQCLGR RLAEVEMLLL LHHMLKTFQV ETLRQEDVQM 

       490        500 
AYRFVLMPSS SPVLTFRPIS 

« Hide

References

[1]"Molecular cloning and expression of cDNAS encoding rat aldosterone synthase: variants of cytochrome P-450(11 beta)."
Matsukawa N., Nonaka Y., Ying Z., Higaki J., Ogihara T., Okamoto M.
Biochem. Biophys. Res. Commun. 169:245-252(1990) [PubMed: 2350348] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Adrenal gland.
[2]"Molecular biology of rat steroid 11 beta-hydroxylase [P450(11 beta)] and aldosterone synthase [P450(11 beta, aldo)]."
Okamoto M., Nonaka Y.
J. Steroid Biochem. Mol. Biol. 41:415-419(1992) [PubMed: 1562515] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[3]"Molecular cloning of a cDNA encoding aldosterone synthase cytochrome P-450 in rat adrenal cortex."
Imai M., Shimada H., Okada Y., Matsushima-Hibiya Y., Ogishima T., Ishimura Y.
FEBS Lett. 263:299-302(1990) [PubMed: 2129527] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Adrenal gland.
[4]"Isolation and characterization of rat CYP11B genes involved in late steps of mineralo- and glucocorticoid syntheses."
Mukai K., Imai M., Shimada H., Ishimura Y.
J. Biol. Chem. 268:9130-9137(1993) [PubMed: 8473352] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[5]"Three forms of rat CYP11B genes: 11 beta-hydroxylase gene, aldosterone synthase gene, and a novel gene."
Nomura M., Morohashi K., Kirita S., Nonaka Y., Okamoto M., Nawata H., Omura T.
J. Biochem. 113:144-152(1993) [PubMed: 8468320] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[6]"Isolation of aldosterone synthase cytochrome P-450 from zona glomerulosa mitochondria of rat adrenal cortex."
Ogishima T., Mitani F., Ishimura Y.
J. Biol. Chem. 264:10935-10938(1989) [PubMed: 2738055] [Abstract]
Cited for: PROTEIN SEQUENCE OF 25-44.
Strain: Sprague-Dawley.
Tissue: Adrenal gland.
+Additional computationally mapped references.

Cross-references

Sequence databases

D00568 mRNA. Translation: BAA00445.1.
X52766 mRNA. Translation: CAA36978.1.
D14097 Genomic DNA. Translation: BAA03172.1.
IPIIPI00205747.
PIRJX0252.
UniGeneRn.144549

3D structure databases

HSSPHSSP built from PDB template 1SCC based on UniProtKB P00189.
ModBaseSearch...

Genome annotation databases

EnsemblENSRNOG00000030111. Rattus norvegicus. [Contig view]
KEGGrno:24294.

Organism-specific databases

RGD727886. Cyp11b3.

Phylogenomic databases

HOVERGENP30100.

Enzyme and pathway databases

BRENDA1.14.15.4. 248.
1.14.15.5. 248.

Gene expression databases

ArrayExpressP30100.
GermOnlineENSRNOG00000030111. Rattus norvegicus.

Family and domain databases

InterProIPR001128. Cyt_P450.
IPR017973. Cyt_P450_C.
IPR017972. Cyt_P450_CS.
IPR002399. Cyt_P450_mit_N.
[Graphical view]
Gene3DG3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHERPTHR19383. Cyt_P450. 1 hit.
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00408. MITP450.
PR00385. P450.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio602896.

Entry information

Entry nameC11B3_RAT
AccessionPrimary (citable) accession number: P30100
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: May 26, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents