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Protein

Cytochrome P450 11B3, mitochondrial

Gene

Cyp11b3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts 11-deoxycorticosterone into corticosterone, 18-hydroxycorticosterone, and aldosterone. Also can catalyze the conversion of 11-deoxycortisol to cortisol, 18-hydroxycortisol and cortisone.

Catalytic activityi

A steroid + 2 reduced adrenodoxin + O2 + 2 H+ = an 11-beta-hydroxysteroid + 2 oxidized adrenodoxin + H2O.By similarity
Corticosterone + 2 reduced adrenodoxin + O2 + 2 H+ = 18-hydroxycorticosterone + 2 oxidized adrenodoxin + H2O.By similarity

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi447Iron (heme axial ligand)By similarity1

GO - Molecular functioni

  • corticosterone 18-monooxygenase activity Source: RGD
  • heme binding Source: RGD
  • iron ion binding Source: InterPro
  • steroid 11-beta-monooxygenase activity Source: RGD

GO - Biological processi

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
Biological processSteroidogenesis
LigandHeme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 11B3, mitochondrial
Alternative name(s):
Aldosterone synthase
CYPXIB3
Cytochrome P450-Aldo-2
Steroid 11-beta-hydroxylase (EC:1.14.15.4, EC:1.14.15.5)
Gene namesi
Name:Cyp11b3
Synonyms:Cyp11b-3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi727886 Cyp11b3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 24Mitochondrion1 PublicationAdd BLAST24
ChainiPRO_000000360525 – 500Cytochrome P450 11B3, mitochondrialAdd BLAST476

Proteomic databases

PaxDbiP30100

Expressioni

Tissue specificityi

Adrenal cortex.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000049722

Structurei

3D structure databases

ProteinModelPortaliP30100
SMRiP30100
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0159 Eukaryota
COG2124 LUCA
HOVERGENiHBG051098
InParanoidiP30100

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR017972 Cyt_P450_CS
IPR002399 Cyt_P450_mitochondrial
IPR036396 Cyt_P450_sf
PfamiView protein in Pfam
PF00067 p450, 1 hit
PRINTSiPR00408 MITP450
PR00385 P450
SUPFAMiSSF48264 SSF48264, 1 hit
PROSITEiView protein in PROSITE
PS00086 CYTOCHROME_P450, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30100-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALRVTADVW LARPWQCLHR TRALGTTATL APKTLKPFEA IPQYSRNKWL
60 70 80 90 100
KMIQILREQG QENLHLEMHQ AFQELGPIFR HSAGGAQIVS VMLPEDAEKL
110 120 130 140 150
HQVESILPRR MHLEPWVAHR ELRGLRRGVF LLNGAEWRFN RLKLNPNVLS
160 170 180 190 200
PKAVQNFVPM VDEVARDFLE ALKKKVRQNA RGSLTMDVQQ SLFNYTIEAS
210 220 230 240 250
NFALFGERLG LLGHDLNPGS LKFIHALHSM FKSTTQLLFL PRSLTRWTST
260 270 280 290 300
QVWKEHFDAW DVISEYANRC IWKVHQELRL GSSQTYSGIV AALITQGALP
310 320 330 340 350
LDAIKANSMK LTAGSVDTTA IPLVMTLFEL ARNPDVQQAL RQETLAAEAS
360 370 380 390 400
IAANPQKAMS DLPLLRAALK ETLRLYPVGG FLERILNSDL VLQNYHVPAG
410 420 430 440 450
TLVLLYLYSM GRNPAVFPRP ERYMPQRWLE RKRSFQHLAF GFGVRQCLGR
460 470 480 490 500
RLAEVEMLLL LHHMLKTFQV ETLRQEDVQM AYRFVLMPSS SPVLTFRPIS
Length:500
Mass (Da):57,122
Last modified:April 1, 1993 - v1
Checksum:i040F6ECCA84CDEAD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti310K → E in CAA36978 (PubMed:2129527).Curated1
Sequence conflicti310K → E in BAA03172 (PubMed:8473352).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00568 mRNA Translation: BAA00445.1
X52766 mRNA Translation: CAA36978.1
D14097 Genomic DNA Translation: BAA03172.1
PIRiJX0252
UniGeneiRn.144549

Genome annotation databases

UCSCiRGD:727886 rat

Similar proteinsi

Entry informationi

Entry nameiC11B3_RAT
AccessioniPrimary (citable) accession number: P30100
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: March 28, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health