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P30100

- C11B3_RAT

UniProt

P30100 - C11B3_RAT

Protein

Cytochrome P450 11B3, mitochondrial

Gene

Cyp11b3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Converts 11-deoxycorticosterone into corticosterone, 18-hydroxycorticosterone, and aldosterone. Also can catalyze the conversion of 11-deoxycortisol to cortisol, 18-hydroxycortisol and cortisone.

    Catalytic activityi

    A steroid + reduced adrenodoxin + O2 = an 11-beta-hydroxysteroid + oxidized adrenodoxin + H2O.
    Corticosterone + reduced adrenodoxin + O2 = 18-hydroxycorticosterone + oxidized adrenodoxin + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi447 – 4471Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. corticosterone 18-monooxygenase activity Source: RGD
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro
    4. steroid 11-beta-monooxygenase activity Source: RGD

    GO - Biological processi

    1. adrenal gland development Source: RGD
    2. response to peptide hormone Source: RGD
    3. steroid biosynthetic process Source: UniProtKB-KW
    4. steroid metabolic process Source: RGD

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Steroidogenesis

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 11B3, mitochondrial
    Alternative name(s):
    Aldosterone synthase
    CYPXIB3
    Cytochrome P450-Aldo-2
    Steroid 11-beta-hydroxylase (EC:1.14.15.4, EC:1.14.15.5)
    Gene namesi
    Name:Cyp11b3
    Synonyms:Cyp11b-3
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi727886. Cyp11b3.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane, Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2424Mitochondrion1 PublicationAdd
    BLAST
    Chaini25 – 500476Cytochrome P450 11B3, mitochondrialPRO_0000003605Add
    BLAST

    Expressioni

    Tissue specificityi

    Adrenal cortex.

    Gene expression databases

    GenevestigatoriP30100.

    Structurei

    3D structure databases

    ProteinModelPortaliP30100.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    HOVERGENiHBG051098.
    InParanoidiP30100.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002399. Cyt_P450_mitochondrial.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00408. MITP450.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P30100-1 [UniParc]FASTAAdd to Basket

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    MALRVTADVW LARPWQCLHR TRALGTTATL APKTLKPFEA IPQYSRNKWL    50
    KMIQILREQG QENLHLEMHQ AFQELGPIFR HSAGGAQIVS VMLPEDAEKL 100
    HQVESILPRR MHLEPWVAHR ELRGLRRGVF LLNGAEWRFN RLKLNPNVLS 150
    PKAVQNFVPM VDEVARDFLE ALKKKVRQNA RGSLTMDVQQ SLFNYTIEAS 200
    NFALFGERLG LLGHDLNPGS LKFIHALHSM FKSTTQLLFL PRSLTRWTST 250
    QVWKEHFDAW DVISEYANRC IWKVHQELRL GSSQTYSGIV AALITQGALP 300
    LDAIKANSMK LTAGSVDTTA IPLVMTLFEL ARNPDVQQAL RQETLAAEAS 350
    IAANPQKAMS DLPLLRAALK ETLRLYPVGG FLERILNSDL VLQNYHVPAG 400
    TLVLLYLYSM GRNPAVFPRP ERYMPQRWLE RKRSFQHLAF GFGVRQCLGR 450
    RLAEVEMLLL LHHMLKTFQV ETLRQEDVQM AYRFVLMPSS SPVLTFRPIS 500
    Length:500
    Mass (Da):57,122
    Last modified:April 1, 1993 - v1
    Checksum:i040F6ECCA84CDEAD
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti310 – 3101K → E in CAA36978. (PubMed:2129527)Curated
    Sequence conflicti310 – 3101K → E in BAA03172. (PubMed:8473352)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D00568 mRNA. Translation: BAA00445.1.
    X52766 mRNA. Translation: CAA36978.1.
    D14097 Genomic DNA. Translation: BAA03172.1.
    PIRiJX0252.
    UniGeneiRn.144549.

    Genome annotation databases

    UCSCiRGD:727886. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D00568 mRNA. Translation: BAA00445.1 .
    X52766 mRNA. Translation: CAA36978.1 .
    D14097 Genomic DNA. Translation: BAA03172.1 .
    PIRi JX0252.
    UniGenei Rn.144549.

    3D structure databases

    ProteinModelPortali P30100.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:727886. rat.

    Organism-specific databases

    RGDi 727886. Cyp11b3.

    Phylogenomic databases

    HOVERGENi HBG051098.
    InParanoidi P30100.

    Gene expression databases

    Genevestigatori P30100.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002399. Cyt_P450_mitochondrial.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00408. MITP450.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and expression of cDNAS encoding rat aldosterone synthase: variants of cytochrome P-450(11 beta)."
      Matsukawa N., Nonaka Y., Ying Z., Higaki J., Ogihara T., Okamoto M.
      Biochem. Biophys. Res. Commun. 169:245-252(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Adrenal gland.
    2. "Molecular biology of rat steroid 11 beta-hydroxylase [P450(11 beta)] and aldosterone synthase [P450(11 beta, aldo)]."
      Okamoto M., Nonaka Y.
      J. Steroid Biochem. Mol. Biol. 41:415-419(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    3. "Molecular cloning of a cDNA encoding aldosterone synthase cytochrome P-450 in rat adrenal cortex."
      Imai M., Shimada H., Okada Y., Matsushima-Hibiya Y., Ogishima T., Ishimura Y.
      FEBS Lett. 263:299-302(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Adrenal gland.
    4. "Isolation and characterization of rat CYP11B genes involved in late steps of mineralo- and glucocorticoid syntheses."
      Mukai K., Imai M., Shimada H., Ishimura Y.
      J. Biol. Chem. 268:9130-9137(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    5. "Three forms of rat CYP11B genes: 11 beta-hydroxylase gene, aldosterone synthase gene, and a novel gene."
      Nomura M., Morohashi K., Kirita S., Nonaka Y., Okamoto M., Nawata H., Omura T.
      J. Biochem. 113:144-152(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    6. "Isolation of aldosterone synthase cytochrome P-450 from zona glomerulosa mitochondria of rat adrenal cortex."
      Ogishima T., Mitani F., Ishimura Y.
      J. Biol. Chem. 264:10935-10938(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 25-44.
      Strain: Sprague-Dawley.
      Tissue: Adrenal gland.

    Entry informationi

    Entry nameiC11B3_RAT
    AccessioniPrimary (citable) accession number: P30100
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3