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P30099

- C11B2_RAT

UniProt

P30099 - C11B2_RAT

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Protein
Cytochrome P450 11B2, mitochondrial
Gene
Cyp11b2, Cyp11b-2
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Converts 11-deoxycorticosterone into corticosterone, 18-hydroxycorticosterone, and aldosterone. Also can catalyze the conversion of 11-deoxycortisol to cortisol, 18-hydroxycortisol and cortisone.

Catalytic activityi

A steroid + reduced adrenodoxin + O2 = an 11-beta-hydroxysteroid + oxidized adrenodoxin + H2O.
Corticosterone + reduced adrenodoxin + O2 = 18-hydroxycorticosterone + oxidized adrenodoxin + H2O.

Cofactori

Heme group By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi457 – 4571Iron (heme axial ligand) By similarity

GO - Molecular functioni

  1. corticosterone 18-monooxygenase activity Source: RGD
  2. heme binding Source: UniProtKB
  3. iron ion binding Source: InterPro
  4. steroid 11-beta-monooxygenase activity Source: RGD

GO - Biological processi

  1. C21-steroid hormone biosynthetic process Source: RGD
  2. aldosterone biosynthetic process Source: RGD
  3. cellular response to mechanical stimulus Source: RGD
  4. cellular response to nutrient Source: RGD
  5. cellular response to peptide hormone stimulus Source: RGD
  6. cellular response to potassium ion starvation Source: RGD
  7. glucocorticoid biosynthetic process Source: RGD
  8. positive regulation of blood pressure Source: RGD
  9. response to drug Source: RGD
  10. response to insulin Source: RGD
  11. response to nutrient Source: RGD
  12. response to peptide hormone Source: RGD
  13. response to salt stress Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Steroidogenesis

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 11B2, mitochondrial
Alternative name(s):
Aldosterone synthase
CYPXIB2
Cytochrome P450-Aldo-1
Steroid 11-beta-hydroxylase (EC:1.14.15.4, EC:1.14.15.5)
Gene namesi
Name:Cyp11b2
Synonyms:Cyp11b-2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2454. Cyp11b2.

Subcellular locationi

GO - Cellular componenti

  1. dendrite Source: RGD
  2. mitochondrial membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3434Mitochondrion1 Publication
Add
BLAST
Chaini35 – 510476Cytochrome P450 11B2, mitochondrial
PRO_0000003604Add
BLAST

Proteomic databases

PaxDbiP30099.
PRIDEiP30099.

Expressioni

Tissue specificityi

Adrenal cortex.

Inductioni

A 12-fold increase was seen in the presence of a low sodium-high potassium diet.1 Publication

Gene expression databases

GenevestigatoriP30099.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000049722.

Structurei

3D structure databases

ProteinModelPortaliP30099.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000013161.
HOVERGENiHBG051098.
InParanoidiP30099.
PhylomeDBiP30099.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002399. Cyt_P450_mitochondrial.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00408. MITP450.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30099-1 [UniParc]FASTAAdd to Basket

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MGACDNDFIE LHSRVTADVW LARPWQCLHR TRALGTTATL APKTLKPFEA    50
IPQYSRNKWL KMIQILREQG QENLHLEMHQ AFQELGPIFR HSAGGAQIVS 100
VMLPEDAEKL HQVESILPRR MHLEPWVAHR ELRGLRRGVF LLNGAEWRFN 150
RLKLNPNVLS PKAVQNFVPM VDEVARDFLE ALKKKVRQNA RGSLTMDVQQ 200
SLFNYTIEAS NFALFGERLG LLGHDLNPGS LKFIHALHSM FKSTTQLLFL 250
PRSLTRWTST QVWKEHFDAW DVISEYANRC IWKVHQELRL GSSQTYSGIV 300
AALITQGALP LDAIKANSME LTAGSVDTTA IPLVMTLFEL ARNPDVQQAL 350
RQETLAAEAS IAANPQKAMS DLPLLRAALK ETLRLYPVGG FLERILNSDL 400
VLQNYHVPAG TLVLLYLYSM GRNPAVFPRP ERYMPQRWLE RKRSFQHLAF 450
GFGVRQCLGR RLAEVEMLLL LHHMLKTFQV ETLRQEDVQM AYRFVLMPSS 500
SPVLTFRPIS 510
Length:510
Mass (Da):58,241
Last modified:April 1, 1993 - v1
Checksum:i2E5129EE513DEA9E
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti84 – 841E → G.1 Publication
Natural varianti146 – 1461E → D.
Natural varianti261 – 2611Q → R.
Natural varianti509 – 5091I → V.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 1313MGACD…IELHS → MNKAPAKAL in AAB60457. 1 Publication
Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D00567 mRNA. Translation: BAA00444.1.
U14908 mRNA. Translation: AAB60457.1.
PIRiA35342.
JN0615.
UniGeneiRn.144549.

Genome annotation databases

UCSCiRGD:2454. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D00567 mRNA. Translation: BAA00444.1 .
U14908 mRNA. Translation: AAB60457.1 .
PIRi A35342.
JN0615.
UniGenei Rn.144549.

3D structure databases

ProteinModelPortali P30099.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000049722.

Chemistry

ChEMBLi CHEMBL3237.

Proteomic databases

PaxDbi P30099.
PRIDEi P30099.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:2454. rat.

Organism-specific databases

RGDi 2454. Cyp11b2.

Phylogenomic databases

eggNOGi COG2124.
HOGENOMi HOG000013161.
HOVERGENi HBG051098.
InParanoidi P30099.
PhylomeDBi P30099.

Gene expression databases

Genevestigatori P30099.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002399. Cyt_P450_mitochondrial.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00408. MITP450.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and expression of cDNAS encoding rat aldosterone synthase: variants of cytochrome P-450(11 beta)."
    Matsukawa N., Nonaka Y., Ying Z., Higaki J., Ogihara T., Okamoto M.
    Biochem. Biophys. Res. Commun. 169:245-252(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Adrenal gland.
  2. "Molecular biology of rat steroid 11 beta-hydroxylase [P450(11 beta)] and aldosterone synthase [P450(11 beta, aldo)]."
    Okamoto M., Nonaka Y.
    J. Steroid Biochem. Mol. Biol. 41:415-419(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Cloning and expression of a rat cytochrome P-450 11 beta-hydroxylase/aldosterone synthase (CYP11B2) cDNA variant."
    Zhou M., Gomez-Sanchez C.E.
    Biochem. Biophys. Res. Commun. 194:112-117(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Adrenal gland.
  4. "Three forms of rat CYP11B genes: 11 beta-hydroxylase gene, aldosterone synthase gene, and a novel gene."
    Nomura M., Morohashi K., Kirita S., Nonaka Y., Okamoto M., Nawata H., Omura T.
    J. Biochem. 113:144-152(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, VARIANT GLY-84.
  5. "Isolation of aldosterone synthase cytochrome P-450 from zona glomerulosa mitochondria of rat adrenal cortex."
    Ogishima T., Mitani F., Ishimura Y.
    J. Biol. Chem. 264:10935-10938(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 35-54.
    Tissue: Adrenal cortex.

Entry informationi

Entry nameiC11B2_RAT
AccessioniPrimary (citable) accession number: P30099
Secondary accession number(s): Q64540
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: April 16, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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