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P30099

- C11B2_RAT

UniProt

P30099 - C11B2_RAT

Protein

Cytochrome P450 11B2, mitochondrial

Gene

Cyp11b2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Converts 11-deoxycorticosterone into corticosterone, 18-hydroxycorticosterone, and aldosterone. Also can catalyze the conversion of 11-deoxycortisol to cortisol, 18-hydroxycortisol and cortisone.

    Catalytic activityi

    A steroid + reduced adrenodoxin + O2 = an 11-beta-hydroxysteroid + oxidized adrenodoxin + H2O.
    Corticosterone + reduced adrenodoxin + O2 = 18-hydroxycorticosterone + oxidized adrenodoxin + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi457 – 4571Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. corticosterone 18-monooxygenase activity Source: RGD
    2. heme binding Source: UniProtKB
    3. iron ion binding Source: InterPro
    4. steroid 11-beta-monooxygenase activity Source: RGD

    GO - Biological processi

    1. aldosterone biosynthetic process Source: RGD
    2. C21-steroid hormone biosynthetic process Source: RGD
    3. cellular response to mechanical stimulus Source: RGD
    4. cellular response to nutrient Source: RGD
    5. cellular response to peptide hormone stimulus Source: RGD
    6. cellular response to potassium ion starvation Source: RGD
    7. glucocorticoid biosynthetic process Source: RGD
    8. positive regulation of blood pressure Source: RGD
    9. response to drug Source: RGD
    10. response to insulin Source: RGD
    11. response to nutrient Source: RGD
    12. response to peptide hormone Source: RGD
    13. response to salt stress Source: RGD

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Steroidogenesis

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 11B2, mitochondrial
    Alternative name(s):
    Aldosterone synthase
    CYPXIB2
    Cytochrome P450-Aldo-1
    Steroid 11-beta-hydroxylase (EC:1.14.15.4, EC:1.14.15.5)
    Gene namesi
    Name:Cyp11b2
    Synonyms:Cyp11b-2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi2454. Cyp11b2.

    Subcellular locationi

    GO - Cellular componenti

    1. dendrite Source: RGD
    2. mitochondrial membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane, Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3434Mitochondrion1 PublicationAdd
    BLAST
    Chaini35 – 510476Cytochrome P450 11B2, mitochondrialPRO_0000003604Add
    BLAST

    Proteomic databases

    PaxDbiP30099.
    PRIDEiP30099.

    Expressioni

    Tissue specificityi

    Adrenal cortex.

    Inductioni

    A 12-fold increase was seen in the presence of a low sodium-high potassium diet.1 Publication

    Gene expression databases

    GenevestigatoriP30099.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000049722.

    Structurei

    3D structure databases

    ProteinModelPortaliP30099.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG2124.
    HOGENOMiHOG000013161.
    HOVERGENiHBG051098.
    InParanoidiP30099.
    PhylomeDBiP30099.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002399. Cyt_P450_mitochondrial.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00408. MITP450.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P30099-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGACDNDFIE LHSRVTADVW LARPWQCLHR TRALGTTATL APKTLKPFEA    50
    IPQYSRNKWL KMIQILREQG QENLHLEMHQ AFQELGPIFR HSAGGAQIVS 100
    VMLPEDAEKL HQVESILPRR MHLEPWVAHR ELRGLRRGVF LLNGAEWRFN 150
    RLKLNPNVLS PKAVQNFVPM VDEVARDFLE ALKKKVRQNA RGSLTMDVQQ 200
    SLFNYTIEAS NFALFGERLG LLGHDLNPGS LKFIHALHSM FKSTTQLLFL 250
    PRSLTRWTST QVWKEHFDAW DVISEYANRC IWKVHQELRL GSSQTYSGIV 300
    AALITQGALP LDAIKANSME LTAGSVDTTA IPLVMTLFEL ARNPDVQQAL 350
    RQETLAAEAS IAANPQKAMS DLPLLRAALK ETLRLYPVGG FLERILNSDL 400
    VLQNYHVPAG TLVLLYLYSM GRNPAVFPRP ERYMPQRWLE RKRSFQHLAF 450
    GFGVRQCLGR RLAEVEMLLL LHHMLKTFQV ETLRQEDVQM AYRFVLMPSS 500
    SPVLTFRPIS 510
    Length:510
    Mass (Da):58,241
    Last modified:April 1, 1993 - v1
    Checksum:i2E5129EE513DEA9E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1 – 1313MGACD…IELHS → MNKAPAKAL in AAB60457. (PubMed:8333830)CuratedAdd
    BLAST

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti84 – 841E → G.1 Publication
    Natural varianti146 – 1461E → D.
    Natural varianti261 – 2611Q → R.
    Natural varianti509 – 5091I → V.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D00567 mRNA. Translation: BAA00444.1.
    U14908 mRNA. Translation: AAB60457.1.
    PIRiA35342.
    JN0615.
    UniGeneiRn.144549.

    Genome annotation databases

    UCSCiRGD:2454. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D00567 mRNA. Translation: BAA00444.1 .
    U14908 mRNA. Translation: AAB60457.1 .
    PIRi A35342.
    JN0615.
    UniGenei Rn.144549.

    3D structure databases

    ProteinModelPortali P30099.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000049722.

    Chemistry

    ChEMBLi CHEMBL3237.

    Proteomic databases

    PaxDbi P30099.
    PRIDEi P30099.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:2454. rat.

    Organism-specific databases

    RGDi 2454. Cyp11b2.

    Phylogenomic databases

    eggNOGi COG2124.
    HOGENOMi HOG000013161.
    HOVERGENi HBG051098.
    InParanoidi P30099.
    PhylomeDBi P30099.

    Gene expression databases

    Genevestigatori P30099.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002399. Cyt_P450_mitochondrial.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00408. MITP450.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and expression of cDNAS encoding rat aldosterone synthase: variants of cytochrome P-450(11 beta)."
      Matsukawa N., Nonaka Y., Ying Z., Higaki J., Ogihara T., Okamoto M.
      Biochem. Biophys. Res. Commun. 169:245-252(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Adrenal gland.
    2. "Molecular biology of rat steroid 11 beta-hydroxylase [P450(11 beta)] and aldosterone synthase [P450(11 beta, aldo)]."
      Okamoto M., Nonaka Y.
      J. Steroid Biochem. Mol. Biol. 41:415-419(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Cloning and expression of a rat cytochrome P-450 11 beta-hydroxylase/aldosterone synthase (CYP11B2) cDNA variant."
      Zhou M., Gomez-Sanchez C.E.
      Biochem. Biophys. Res. Commun. 194:112-117(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Adrenal gland.
    4. "Three forms of rat CYP11B genes: 11 beta-hydroxylase gene, aldosterone synthase gene, and a novel gene."
      Nomura M., Morohashi K., Kirita S., Nonaka Y., Okamoto M., Nawata H., Omura T.
      J. Biochem. 113:144-152(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, VARIANT GLY-84.
    5. "Isolation of aldosterone synthase cytochrome P-450 from zona glomerulosa mitochondria of rat adrenal cortex."
      Ogishima T., Mitani F., Ishimura Y.
      J. Biol. Chem. 264:10935-10938(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 35-54.
      Tissue: Adrenal cortex.

    Entry informationi

    Entry nameiC11B2_RAT
    AccessioniPrimary (citable) accession number: P30099
    Secondary accession number(s): Q64540
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3