P30098 (NK3R_CAVPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neuromedin-K receptor
Short name=NKR

Alternative name(s):
NK-3 receptor
Short name=NK-3R
Neurokinin B receptor
Tachykinin receptor 3

Gene names

Name:

TACR3

Organism

Cavia porcellus (Guinea pig)

Taxonomic identifier

10141 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Caviidae › Cavia

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This is a receptor for the tachykinin neuropeptide neuromedin-K (neurokinin B). It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Post-translational modification	The anchoring of this receptor to the plasma membrane is probably mediated by the palmitoylation of a cysteine residue.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.
Caution	Was originally (Ref.1) thought to be a kappa-type opioid receptor and to originate from human. Ref.2 showed that it is a tachikinin receptor and was termed NK-4R. Ref.3 shows that it is from guinea pig and is NK-3R.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Gene Ontology (GO)
Cellular component	integral to plasma membrane Traceable author statement. Source: ProtInc
Molecular function	tachykinin receptor activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 440	440	Neuromedin-K receptor		PRO_0000069898
Regions
	Topological domain	1 – 59	59	Extracellular Potential
	Transmembrane	60 – 82	23	Helical; Name=1; Potential
	Topological domain	83 – 92	10	Cytoplasmic Potential
	Transmembrane	93 – 114	22	Helical; Name=2; Potential
	Topological domain	115 – 134	20	Extracellular Potential
	Transmembrane	135 – 156	22	Helical; Name=3; Potential
	Topological domain	157 – 176	20	Cytoplasmic Potential
	Transmembrane	177 – 197	21	Helical; Name=4; Potential
	Topological domain	198 – 220	23	Extracellular Potential
	Transmembrane	221 – 245	25	Helical; Name=5; Potential
	Topological domain	246 – 274	29	Cytoplasmic Potential
	Transmembrane	275 – 296	22	Helical; Name=6; Potential
	Topological domain	297 – 309	13	Extracellular Potential
	Transmembrane	310 – 334	25	Helical; Name=7; Potential
	Topological domain	335 – 440	106	Cytoplasmic Potential
Amino acid modifications
	Lipidation	349	1	S-palmitoyl cysteine By similarity
	Glycosylation	7	1	N-linked (GlcNAc...) Potential
	Glycosylation	24	1	N-linked (GlcNAc...) Potential
	Disulfide bond	133 ↔ 208		By similarity
Experimental info
	Sequence conflict	59	1	A → R in AAK49193. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P30098 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 544EB3A71CC6C143	FASTA	440	49,432
	10 20 30 40 50 60 MASPAGNLSA WPGWGWPPPA ALRNLTSSPA PTASPSPAPS WTPSPRPGPA HPFLQPPWAV 70 80 90 100 110 120 ALWSLAYGAV VAVAVLGNLV VIWIVLAHKR MRTVTNSFLV NLAFADAAMA ALNALVNFIY 130 140 150 160 170 180 ALHGEWYFGA NYCRFQNFFP ITAVFASIYS MTAIAVDRYM AIIDPLKPRL SATATRIVIG 190 200 210 220 230 240 SIWILAFLLA FPQCLYSKIK VMPGRTLCYV QWPEGSRQHF TYHMIVIVLV YCFPLLIMGI 250 260 270 280 290 300 TYTIVGITLW GGEIPGDTCD KYQEQLKAKR KVVKMMIIVV VTFAICWLPY HIYFILTAIY 310 320 330 340 350 360 QQLNRWKYIQ QVYLASFWLA MSSTMYNPII YCCLNKRFRA GFKRAFRWCP FIHVSSYDEL 370 380 390 400 410 420 ELKATRLHPM RQSSLYTVTR MESMSVVFDS NDGDSARSSH QKRGTTRDVG SNVCSRRNSK 430 440 STSTTASFVS SSHMSVEEGS

References

[1]	"Expression cloning of cDNA encoding a seven-helix receptor from human placenta with affinity for opioid ligands." Xie G.-X., Miyajima A., Goldstein A. Proc. Natl. Acad. Sci. U.S.A. 89:4124-4128(1992) [PubMed: 1315051] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta.
[2]	"Functional characterization by heterologous expression of a novel cloned tachykinin peptide receptor." Donaldson L.F., Haskell C.A., Hanley M.R. Biochem. J. 320:1-5(1996) [PubMed: 8947459] [Abstract] Cited for: SHOWS THAT IT IS NOT AN OPIOID RECEPTOR.
[3]	"The human tachykinin NK1 (short form) and tachykinin NK4 receptor: a reappraisal." Page N.M., Bell N.J. Eur. J. Pharmacol. 437:27-30(2002) [PubMed: 11864635] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SHOWS THAT IT IS NOT A HUMAN SEQUENCE.
[4]	"Cloning and sequencing of guinea pig NK3 receptor." Stumm R.K., Derst C., Schaefer M.K.H., Weihe E. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M84605 mRNA. Translation: AAA36395.1. AF426173 mRNA. Translation: AAL78507.1. AF255393 mRNA. Translation: AAK49193.1.
PIR	A44081.
RefSeq	NP_001166200.1. NM_001172729.1.
3D structure databases
ProteinModelPortal	P30098.
ModBase	Search...
Protein-protein interaction databases
STRING	P30098.
Protein family/group databases
GPCRDB	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSCPOT00000027665; ENSCPOP00000014870; ENSCPOG00000010746.
GeneID	100135627.
Organism-specific databases
CTD	6870.
Phylogenomic databases
eggNOG	roNOG11982.
GeneTree	ENSGT00560000076767.
HOVERGEN	HBG103412.
InParanoid	P30098.
Family and domain databases
InterPro	IPR000276. 7TM_GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR001681. Neurokn_rcpt. IPR001013. NK3_rcpt. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR01026. NEUROKININ3R. PR00244. NEUROKININR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NK3R_CAVPO

Accession

Primary (citable) accession number: P30098
Secondary accession number(s): Q925R4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	September 21, 2011
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program