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Protein

Phosphatidylethanolamine-binding protein 1

Gene

PEBP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase (By similarity). Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation.By similarity1 Publication
HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor (By similarity).By similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • enzyme binding Source: UniProtKB
  • phosphatidylethanolamine binding Source: ProtInc
  • poly(A) RNA binding Source: UniProtKB
  • protein kinase binding Source: UniProtKB
  • serine-type endopeptidase inhibitor activity Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Keywords - Ligandi

ATP-binding, Lipid-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000089220-MONOMER.
ReactomeiR-HSA-5674135. MAP2K and MAPK activation.
R-HSA-5675221. Negative regulation of MAPK pathway.
R-HSA-6802946. Signaling by moderate kinase activity BRAF mutants.
R-HSA-6802948. Signaling by high-kinase activity BRAF mutants.
R-HSA-6802949. Signaling by RAS mutants.
R-HSA-6802952. Signaling by BRAF and RAF fusions.
R-HSA-6802955. Paradoxical activation of RAF signaling by kinase inactive BRAF.
SignaLinkiP30086.
SIGNORiP30086.

Protein family/group databases

MEROPSiI51.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylethanolamine-binding protein 1
Short name:
PEBP-1
Alternative name(s):
HCNPpp
Neuropolypeptide h3
Prostatic-binding protein
Raf kinase inhibitor protein
Short name:
RKIP
Cleaved into the following chain:
Gene namesi
Name:PEBP1
Synonyms:PBP, PEBP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:8630. PEBP1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi5037.
OpenTargetsiENSG00000089220.
PharmGKBiPA32968.

Polymorphism and mutation databases

BioMutaiPEBP1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000232712 – 187Phosphatidylethanolamine-binding protein 1Add BLAST186
PeptideiPRO_00000232722 – 12Hippocampal cholinergic neurostimulating peptideAdd BLAST11

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei6PhosphoserineCombined sources1
Modified residuei13PhosphoserineBy similarity1
Modified residuei42PhosphothreonineCombined sources1
Modified residuei52PhosphoserineCombined sources1
Modified residuei54PhosphoserineCombined sources1
Modified residuei98PhosphoserineCombined sources1
Modified residuei153PhosphoserineCombined sources1

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

EPDiP30086.
MaxQBiP30086.
PaxDbiP30086.
PeptideAtlasiP30086.
PRIDEiP30086.
TopDownProteomicsiP30086.

2D gel databases

DOSAC-COBS-2DPAGEP30086.
OGPiP30086.
REPRODUCTION-2DPAGEIPI00219446.
P30086.
SWISS-2DPAGEP30086.
UCD-2DPAGEP30086.

PTM databases

iPTMnetiP30086.
PhosphoSitePlusiP30086.
SwissPalmiP30086.

Expressioni

Gene expression databases

BgeeiENSG00000089220.
CleanExiHS_PEBP1.
ExpressionAtlasiP30086. baseline and differential.
GenevisibleiP30086. HS.

Organism-specific databases

HPAiCAB009906.
CAB013493.
HPA008819.

Interactioni

Subunit structurei

Has a tendency to form dimers by disulfide cross-linking (By similarity). Interacts with RAF1 and this interaction is enhanced if RAF1 is phosphorylated on residues 'Ser-338', 'Ser-339', 'Tyr-340' and 'Tyr-341'. Interacts with ALOX15; in response to IL13/interleukin-13, prevents the interaction of PEBP1 with RAF1 to activate the ERK signaling cascade.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RAF1P040497EBI-716384,EBI-365996

GO - Molecular functioni

  • enzyme binding Source: UniProtKB
  • protein kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi111076. 39 interactors.
DIPiDIP-44269N.
IntActiP30086. 10 interactors.
MINTiMINT-5002544.
STRINGi9606.ENSP00000261313.

Structurei

Secondary structure

1187
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 7Combined sources3
Turni8 – 12Combined sources5
Helixi14 – 16Combined sources3
Beta strandi22 – 24Combined sources3
Beta strandi26 – 28Combined sources3
Beta strandi32 – 34Combined sources3
Turni43 – 46Combined sources4
Beta strandi51 – 54Combined sources4
Beta strandi62 – 71Combined sources10
Beta strandi76 – 78Combined sources3
Beta strandi84 – 93Combined sources10
Helixi97 – 99Combined sources3
Beta strandi100 – 104Combined sources5
Beta strandi118 – 129Combined sources12
Beta strandi140 – 142Combined sources3
Turni144 – 146Combined sources3
Helixi151 – 157Combined sources7
Beta strandi164 – 171Combined sources8
Helixi177 – 183Combined sources7
Turni184 – 186Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BD9X-ray2.05A/B1-187[»]
1BEHX-ray1.75A/B1-187[»]
2L7WNMR-A1-187[»]
2QYQX-ray1.95A1-187[»]
ProteinModelPortaliP30086.
SMRiP30086.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP30086.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni93 – 134Interaction with RAF11 PublicationAdd BLAST42

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3346. Eukaryota.
COG1881. LUCA.
GeneTreeiENSGT00840000129869.
HOGENOMiHOG000237655.
HOVERGENiHBG008165.
InParanoidiP30086.
OMAiSIEWEGC.
OrthoDBiEOG091G0KH6.
PhylomeDBiP30086.
TreeFamiTF315074.

Family and domain databases

Gene3Di3.90.280.10. 1 hit.
InterProiIPR008914. PEBP.
IPR001858. Phosphotidylethanolamine-bd_CS.
[Graphical view]
PfamiPF01161. PBP. 1 hit.
[Graphical view]
SUPFAMiSSF49777. SSF49777. 1 hit.
PROSITEiPS01220. PBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30086-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVDLSKWSG PLSLQEVDEQ PQHPLHVTYA GAAVDELGKV LTPTQVKNRP
60 70 80 90 100
TSISWDGLDS GKLYTLVLTD PDAPSRKDPK YREWHHFLVV NMKGNDISSG
110 120 130 140 150
TVLSDYVGSG PPKGTGLHRY VWLVYEQDRP LKCDEPILSN RSGDHRGKFK
160 170 180
VASFRKKYEL RAPVAGTCYQ AEWDDYVPKL YEQLSGK
Length:187
Mass (Da):21,057
Last modified:January 23, 2007 - v3
Checksum:iF1E9F17E2CD11C36
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8W → K (PubMed:7637590).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0060489S → N.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16111 mRNA. Translation: BAA03684.1.
X75252 mRNA. Translation: CAA53031.1.
X85033 mRNA. Translation: CAA59404.1.
AK311927 mRNA. Translation: BAG34868.1.
CH471054 Genomic DNA. Translation: EAW98122.1.
BC008714 mRNA. Translation: AAH08714.1.
BC017396 mRNA. Translation: AAH17396.1.
BC031102 mRNA. Translation: AAH31102.1.
S76773 mRNA. Translation: AAD14234.1.
CCDSiCCDS9187.1.
PIRiI53745.
RefSeqiNP_002558.1. NM_002567.3.
UniGeneiHs.433863.

Genome annotation databases

EnsembliENST00000261313; ENSP00000261313; ENSG00000089220.
GeneIDi5037.
KEGGihsa:5037.
UCSCiuc001twu.2. human.

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16111 mRNA. Translation: BAA03684.1.
X75252 mRNA. Translation: CAA53031.1.
X85033 mRNA. Translation: CAA59404.1.
AK311927 mRNA. Translation: BAG34868.1.
CH471054 Genomic DNA. Translation: EAW98122.1.
BC008714 mRNA. Translation: AAH08714.1.
BC017396 mRNA. Translation: AAH17396.1.
BC031102 mRNA. Translation: AAH31102.1.
S76773 mRNA. Translation: AAD14234.1.
CCDSiCCDS9187.1.
PIRiI53745.
RefSeqiNP_002558.1. NM_002567.3.
UniGeneiHs.433863.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BD9X-ray2.05A/B1-187[»]
1BEHX-ray1.75A/B1-187[»]
2L7WNMR-A1-187[»]
2QYQX-ray1.95A1-187[»]
ProteinModelPortaliP30086.
SMRiP30086.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111076. 39 interactors.
DIPiDIP-44269N.
IntActiP30086. 10 interactors.
MINTiMINT-5002544.
STRINGi9606.ENSP00000261313.

Protein family/group databases

MEROPSiI51.002.

PTM databases

iPTMnetiP30086.
PhosphoSitePlusiP30086.
SwissPalmiP30086.

Polymorphism and mutation databases

BioMutaiPEBP1.

2D gel databases

DOSAC-COBS-2DPAGEP30086.
OGPiP30086.
REPRODUCTION-2DPAGEIPI00219446.
P30086.
SWISS-2DPAGEP30086.
UCD-2DPAGEP30086.

Proteomic databases

EPDiP30086.
MaxQBiP30086.
PaxDbiP30086.
PeptideAtlasiP30086.
PRIDEiP30086.
TopDownProteomicsiP30086.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000261313; ENSP00000261313; ENSG00000089220.
GeneIDi5037.
KEGGihsa:5037.
UCSCiuc001twu.2. human.

Organism-specific databases

CTDi5037.
DisGeNETi5037.
GeneCardsiPEBP1.
HGNCiHGNC:8630. PEBP1.
HPAiCAB009906.
CAB013493.
HPA008819.
MIMi604591. gene.
neXtProtiNX_P30086.
OpenTargetsiENSG00000089220.
PharmGKBiPA32968.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3346. Eukaryota.
COG1881. LUCA.
GeneTreeiENSGT00840000129869.
HOGENOMiHOG000237655.
HOVERGENiHBG008165.
InParanoidiP30086.
OMAiSIEWEGC.
OrthoDBiEOG091G0KH6.
PhylomeDBiP30086.
TreeFamiTF315074.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000089220-MONOMER.
ReactomeiR-HSA-5674135. MAP2K and MAPK activation.
R-HSA-5675221. Negative regulation of MAPK pathway.
R-HSA-6802946. Signaling by moderate kinase activity BRAF mutants.
R-HSA-6802948. Signaling by high-kinase activity BRAF mutants.
R-HSA-6802949. Signaling by RAS mutants.
R-HSA-6802952. Signaling by BRAF and RAF fusions.
R-HSA-6802955. Paradoxical activation of RAF signaling by kinase inactive BRAF.
SignaLinkiP30086.
SIGNORiP30086.

Miscellaneous databases

ChiTaRSiPEBP1. human.
EvolutionaryTraceiP30086.
GeneWikiiPhosphatidylethanolamine_binding_protein_1.
GenomeRNAii5037.
PROiP30086.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000089220.
CleanExiHS_PEBP1.
ExpressionAtlasiP30086. baseline and differential.
GenevisibleiP30086. HS.

Family and domain databases

Gene3Di3.90.280.10. 1 hit.
InterProiIPR008914. PEBP.
IPR001858. Phosphotidylethanolamine-bd_CS.
[Graphical view]
PfamiPF01161. PBP. 1 hit.
[Graphical view]
SUPFAMiSSF49777. SSF49777. 1 hit.
PROSITEiPS01220. PBP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPEBP1_HUMAN
AccessioniPrimary (citable) accession number: P30086
Secondary accession number(s): B2R4S1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 179 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.