Reviewed, UniProtKB/Swiss-Prot P30086 (PEBP1_HUMAN)

Names and origin

Protein names

Recommended name:
    Phosphatidylethanolamine-binding protein 1
      Short name=PEBP-1
Alternative name(s):
    Prostatic-binding protein
    HCNPpp
    Neuropolypeptide h3
    Raf kinase inhibitor protein
      Short name=RKIP
Cleaved into the following chain:
    1- Recommended name:
            Hippocampal cholinergic neurostimulating peptide
                Short name=HCNP

Gene names

Name:	PEBP1
Synonyms:	PBP, PEBP

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	187 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase By similarity. HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor By similarity.
Subunit structure	Interacts with Raf-1 and seems to inhibit it. Ref.9
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphatidylethanolamine-binding protein family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Lipid-binding Nucleotide-binding
Molecular function	Protease inhibitor Serine protease inhibitor
PTM	Phosphoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphatidylethanolamine binding Ref.2 Traceable author statement. Source: ProtInc protein binding Inferred from physical interaction. Source: IntAct serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CDK2	P24941	1	EBI-716384,EBI-375096
PACSIN3	Q9UKS6	1	EBI-716384,EBI-77926
PIK3R1	P27986	1	EBI-716384,EBI-79464

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.5

Chain

2 – 187

186

Phosphatidylethanolamine-binding protein 1

PRO_0000023271

Peptide

2 – 12

11

Hippocampal cholinergic neurostimulating peptide

PRO_0000023272

Amino acid modifications

Modified residue

42

1

Phosphothreonine Ref.11

Modified residue

52

1

Phosphoserine Ref.11 Ref.10

Modified residue

54

1

Phosphoserine By similarity

Natural variations

Natural variant

9

1

S → N

VAR_006048

Experimental info

Sequence conflict

8

1

W → K Ref.2

Secondary structure

1

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187

Helix Strand Turn

Details...

Helix

6 – 9

4

Helix

14 – 16

3

Beta strand

22 – 24

3

Beta strand

26 – 29

4

Beta strand

32 – 34

3

Helix

44 – 46

3

Beta strand

51 – 54

4

Beta strand

62 – 70

9

Beta strand

76 – 78

3

Beta strand

84 – 93

10

Helix

97 – 99

3

Beta strand

100 – 104

5

Beta strand

118 – 126

9

Turn

144 – 146

3

Helix

151 – 157

7

Beta strand

164 – 171

8

Helix

177 – 183

7

Sequences

	Sequence		Length	Mass (Da)
	P30086-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: F1E9F17E2CD11C36	FASTA	187	21,057
	10 20 30 40 50 60 MPVDLSKWSG PLSLQEVDEQ PQHPLHVTYA GAAVDELGKV LTPTQVKNRP TSISWDGLDS 70 80 90 100 110 120 GKLYTLVLTD PDAPSRKDPK YREWHHFLVV NMKGNDISSG TVLSDYVGSG PPKGTGLHRY 130 140 150 160 170 180 VWLVYEQDRP LKCDEPILSN RSGDHRGKFK VASFRKKYEL RAPVAGTCYQ AEWDDYVPKL YEQLSGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A human cDNA sequence homologue of bovine phosphatidylethanolamine-binding protein." Hori N., Keon-Sang C., Murakawa K., Matoba R., Fukushima A., Okubo K., Matsubara K. Gene 140:293-294(1994) [PubMed: 8144042] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Sequence homology of rat and human HCNP precursor proteins, bovine phosphatidylethanolamine-binding protein and rat 23-kDa protein associated with the opioid-binding protein." Tohdoh N., Tojo S., Agui H., Ojika K. Brain Res. Mol. Brain Res. 30:381-384(1995) [PubMed: 7637590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta.
[3]	Hall L. Submitted (FEB-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain, Hypothalamus and Lung.
[5]	"Human liver protein map: a reference database established by microsequencing and gel comparison." Hochstrasser D.F., Frutiger S., Paquet N., Bairoch A., Ravier F., Pasquali C., Sanchez J.-C., Tissot J.-D., Bjellqvist B., Vargas R., Appel R.D., Hughes G.J. Electrophoresis 13:992-1001(1992) [PubMed: 1286669] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-11. Tissue: Liver.
[6]	Lubec G., Vishwanath V., Chen W.-Q., Sun Y. Submitted (DEC-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 8-39; 48-77; 81-113; 120-141 AND 162-187, MASS SPECTROMETRY. Tissue: Brain, Cajal-Retzius cell and Fetal brain cortex.
[7]	"Amino acid sequence of the Homo sapiens brain 21-23-kDa protein (neuropolypeptide h3), comparison with its counterparts from Rattus norvegicus and Bos taurus species, and expression of its mRNA in different tissues." Seddiqi N., Bollengier F., Alliel P.M., Perin J.-P., Bonnet F., Bucquoy S., Jolles P., Schoentgen F. J. Mol. Evol. 39:655-660(1994) [PubMed: 7807553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 48-187. Tissue: Brain.
[8]	"Hippocampal cholinergic neurostimulating peptides (HCNP)." Ojika K., Mitake S., Tohdoh N., Appel S.H., Otsuka Y., Katada E., Matsukawa N. Prog. Neurobiol. 60:37-83(2000) [PubMed: 10622376] [Abstract] Cited for: CHARACTERIZATION OF HCNP.
[9]	"Suppression of Raf-1 kinase activity and MAP kinase signalling by RKIP." Yeung K., Seitz T., Li S., Janosch P., McFerran B., Kaiser C., Fee F., Katsanakis K.D., Rose D.W., Mischak H., Sedivy J.M., Kolch W. Nature 401:173-177(1999) [PubMed: 10490027] [Abstract] Cited for: INTERACTION WITH RAF-1.
[10]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-52, MASS SPECTROMETRY. Tissue: Epithelium.
[11]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-42 AND SER-52, MASS SPECTROMETRY.
[12]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[13]	"Function from structure? The crystal structure of human phosphatidylethanolamine-binding protein suggests a role in membrane signal transduction." Banfield M.J., Barker J.J., Perry A.C., Brady R.L. Structure 6:1245-1254(1998) [PubMed: 9782050] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS).
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases

EMBLGenBankDDBJ

D16111 mRNA. Translation: BAA03684.1.
X75252 mRNA. Translation: CAA53031.1.
X85033 mRNA. Translation: CAA59404.1.
BC008714 mRNA. Translation: AAH08714.1.
BC017396 mRNA. Translation: AAH17396.1.
BC031102 mRNA. Translation: AAH31102.1.
S76773 mRNA. Translation: AAD14234.1.

IPI

IPI00219446.

PIR

I53745.

RefSeq

NP_002558.1.

UniGene

Hs.433863
Hs.713526

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BD9	X-ray	2.05	A/B	1-187	[»]
1BEH	X-ray	1.75	A/B	1-187	[»]
2QYQ	X-ray	1.95	A	1-187	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P30086. 13 interactions.

Protein family/group databases

MEROPS

I51.002.

PTM databases

PhosphoSite

P30086.

2-D gel databases

SWISS-2DPAGE

P30086.

DOSAC-COBS-2DPAGE

P30086.

OGP

P30086.

REPRODUCTION-2DPAGE

IPI00219446.
P30086.

Siena-2DPAGE

P30086.

Proteomic databases

PeptideAtlas

P30086.

PRIDE

P30086.

Genome annotation databases

Ensembl

ENSG00000089220. Homo sapiens. [Contig view]

GeneID

5037.

KEGG

hsa:5037.

Organism-specific databases

GeneCards

GC12P117058.

H-InvDB

HIX0011046.

HGNC

HGNC:8630. PEBP1.

HPA

CAB009906.
CAB013493.
HPA008819.

MIM

604591. gene.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

P30086.

HOVERGEN

P30086.

OMA

P30086. QEVDERP.

Enzyme and pathway databases

Pathway_Interaction_DB

aurora_b_pathway. Aurora B signaling.

Gene expression databases

Bgee

P30086.

CleanEx

HS_PEBP1.

GermOnline

ENSG00000089220. Homo sapiens.

Family and domain databases

InterPro

IPR001858. Phosphotidylethanolamine_bd_CS.
IPR008914. PtdEtn-bd_prot_PEBP.
[Graphical view]

Pfam

PF01161. PBP. 1 hit.
[Graphical view]

ProDom

PD004330. PBP. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS01220. PBP. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

19408.

SOURCE

Search...

Entry information

Entry name

PEBP1_HUMAN

Accession

Primary (citable) accession number: P30086

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 101 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)