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P30082 (GLR_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucagon receptor

Short name=GL-R
Gene names
Name:Gcgr
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length485 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

G-protein coupled receptor for glucagon that plays a central role in the regulation of blood glucose levels and glucose homeostasis. Regulates the rate of hepatic glucose production by promoting glycogen hydrolysis and gluconeogenesis. Plays an important role in mediating the responses to fasting. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Promotes activation of adenylate cyclase. Besides, plays a role in signaling via a phosphatidylinositol-calcium second messenger system. Ref.1

Subcellular location

Cell membrane; Multi-pass membrane protein. Note: Is rapidly internalized after ligand-binding By similarity. Ref.1

Post-translational modification

Ligand-binding promotes phosphorylation of serine residues in the C-terminal cytoplasmic domain. Phosphorylation is important for receptor endocytosis after ligand-binding By similarity.

Sequence similarities

Belongs to the G-protein coupled receptor 2 family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor signaling pathway

Inferred from direct assay PubMed 17010343. Source: RGD

adenylate cyclase-activating G-protein coupled receptor signaling pathway

Inferred from direct assay PubMed 15459251. Source: RGD

adenylate cyclase-modulating G-protein coupled receptor signaling pathway

Inferred from mutant phenotype PubMed 8552628. Source: RGD

exocytosis

Inferred from direct assay PubMed 15459251. Source: RGD

glucose homeostasis

Inferred from sequence or structural similarity. Source: UniProtKB

hormone-mediated signaling pathway

Inferred from direct assay PubMed 17010343. Source: RGD

regulation of glycogen metabolic process

Inferred from sequence or structural similarity. Source: UniProtKB

response to starvation

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentendosome

Inferred from direct assay PubMed 17010343. Source: RGD

integral component of membrane

Traceable author statement PubMed 12269822. Source: RGD

integral component of plasma membrane

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Inferred from direct assay PubMed 15687107PubMed 17010343PubMed 8552628. Source: RGD

   Molecular_functionglucagon receptor activity

Inferred from direct assay PubMed 17010343PubMed 8386505. Source: RGD

peptide hormone binding

Inferred from direct assay PubMed 15687107PubMed 17010343. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Chain27 – 485459Glucagon receptor
PRO_0000012834

Regions

Topological domain27 – 137111Extracellular By similarity
Transmembrane138 – 16225Helical; Name=1; By similarity
Topological domain163 – 17412Cytoplasmic By similarity
Transmembrane175 – 19925Helical; Name=2; By similarity
Topological domain200 – 22627Extracellular By similarity
Transmembrane227 – 25024Helical; Name=3; By similarity
Topological domain251 – 26414Cytoplasmic By similarity
Transmembrane265 – 28622Helical; Name=4; By similarity
Topological domain287 – 30418Extracellular By similarity
Transmembrane305 – 32723Helical; Name=5; By similarity
Topological domain328 – 35124Cytoplasmic By similarity
Transmembrane352 – 37019Helical; Name=6; By similarity
Topological domain371 – 38212Extracellular By similarity
Transmembrane383 – 40321Helical; Name=7; By similarity
Topological domain404 – 48582Cytoplasmic By similarity

Amino acid modifications

Glycosylation471N-linked (GlcNAc...) Potential
Glycosylation601N-linked (GlcNAc...) Potential
Glycosylation751N-linked (GlcNAc...) Potential
Glycosylation791N-linked (GlcNAc...) Potential
Disulfide bond44 ↔ 68 By similarity
Disulfide bond59 ↔ 101 By similarity
Disulfide bond82 ↔ 122 By similarity

Experimental info

Sequence conflict2161W → C Ref.2
Sequence conflict3241V → A Ref.2

Sequences

Sequence LengthMass (Da)Tools
P30082 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: 91AC67D7A4F5090E

FASTA48555,038
        10         20         30         40         50         60 
MLLTQLHCPY LLLLLVVLSC LPKAPSAQVM DFLFEKWKLY SDQCHHNLSL LPPPTELVCN 

        70         80         90        100        110        120 
RTFDKYSCWP DTPPNTTANI SCPWYLPWYH KVQHRLVFKR CGPDGQWVRG PRGQSWRDAS 

       130        140        150        160        170        180 
QCQMDDDEIE VQKGVAKMYS SYQVMYTVGY SLSLGALLLA LVILLGLRKL HCTRNYIHGN 

       190        200        210        220        230        240 
LFASFVLKAG SVLVIDWLLK TRYSQKIGDD LSVSVWLSDG AVAGCRVATV IMQYGIIANY 

       250        260        270        280        290        300 
CWLLVEGVYL YSLLSITTFS EKSFFSLYLC IGWGSPLLFV IPWVVVKCLF ENVQCWTSND 

       310        320        330        340        350        360 
NMGFWWILRI PVLLAILINF FIFVRIIHLL VAKLRAHQMH YADYKFRLAR STLTLIPLLG 

       370        380        390        400        410        420 
VHEVVFAFVT DEHAQGTLRS TKLFFDLFFS SFQGLLVAVL YCFLNKEVQA ELLRRWRRWQ 

       430        440        450        460        470        480 
EGKALQEERM ASSHGSHMAP AGTCHGDPCE KLQLMSAGSS SGTGCEPSAK TSLASSLPRL 


ADSPT 

« Hide

References

[1]"Expression cloning and signaling properties of the rat glucagon receptor."
Jelinek L.J., Lok S., Grant F.J., Rosenberg G.B., Smith R.A., Bensch P.A., Sheppard P.O., O'Hara P.J., Foster D.C., Kuijper J.L., Biggs S.H., Walker K.M., Chen L.H., McKernan P.A., Kindsvogel W.
Science 259:1614-1616(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
[2]"Small introns in a hepatic cDNA encoding a new glucagon-like peptide 1-type receptor."
Svoboda M., Ciccarelli E., Tastenoy M., Cauvin A., Stievenart M., Christophe J.
Biochem. Biophys. Res. Commun. 191:479-486(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Sequencing of eleven introns in genomic DNA encoding rat glucagon receptor and multiple alternative splicing of its mRNA."
Maget B., Tastenoy M., Svoboda M.
FEBS Lett. 351:271-275(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Wistar.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L04796 mRNA. Translation: AAA16439.1.
X68692 mRNA. Translation: CAA48651.1.
M96674 mRNA. Translation: AAA02992.1.
U63021 Genomic DNA. Translation: AAB16800.1.
PIRJQ1957.
RefSeqNP_742088.1. NM_172091.2.
NP_742089.1. NM_172092.2.
XP_006247938.1. XM_006247876.1.
UniGeneRn.11225.

3D structure databases

ProteinModelPortalP30082.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4996622.
STRING10116.ENSRNOP00000051845.

Chemistry

BindingDBP30082.
ChEMBLCHEMBL4720.
GuidetoPHARMACOLOGY251.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP30082.

Proteomic databases

PaxDbP30082.
PRIDEP30082.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000054962; ENSRNOP00000051845; ENSRNOG00000036692.
GeneID24953.
KEGGrno:24953.

Organism-specific databases

CTD2642.
RGD2669. Gcgr.

Phylogenomic databases

eggNOGNOG263329.
GeneTreeENSGT00740000115231.
HOGENOMHOG000008250.
HOVERGENHBG008318.
InParanoidP30082.
KOK04583.
OMALPWHHKV.
OrthoDBEOG7TF78W.
PhylomeDBP30082.
TreeFamTF315710.

Gene expression databases

GenevestigatorP30082.

Family and domain databases

InterProIPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR003290. GPCR_2_GLP1/glucagon_rcpt.
IPR003291. GPCR_2_glucagon_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
[Graphical view]
PfamPF00002. 7tm_2. 1 hit.
PF02793. HRM. 1 hit.
[Graphical view]
PRINTSPR01353. GLUCAGNFAMLY.
PR01354. GLUCAGONR.
PR00249. GPCRSECRETIN.
SMARTSM00008. HormR. 1 hit.
[Graphical view]
PROSITEPS00649. G_PROTEIN_RECEP_F2_1. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio604975.
PROP30082.

Entry information

Entry nameGLR_RAT
AccessionPrimary (citable) accession number: P30082
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: February 1, 1996
Last modified: June 11, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries