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Protein

Glucagon receptor

Gene

Gcgr

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

G-protein coupled receptor for glucagon that plays a central role in the regulation of blood glucose levels and glucose homeostasis. Regulates the rate of hepatic glucose production by promoting glycogen hydrolysis and gluconeogenesis. Plays an important role in mediating the responses to fasting. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Promotes activation of adenylate cyclase. Besides, plays a role in signaling via a phosphatidylinositol-calcium second messenger system.1 Publication

GO - Molecular functioni

  • glucagon receptor activity Source: RGD
  • peptide hormone binding Source: RGD

GO - Biological processi

  • adenylate cyclase-activating G-protein coupled receptor signaling pathway Source: RGD
  • adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: RGD
  • exocytosis Source: RGD
  • glucose homeostasis Source: UniProtKB
  • G-protein coupled receptor signaling pathway Source: RGD
  • hormone-mediated signaling pathway Source: RGD
  • regulation of glycogen metabolic process Source: UniProtKB
  • response to starvation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_287445. G alpha (s) signalling events.
REACT_311068. Glucagon signaling in metabolic regulation.
REACT_326473. Glucagon-type ligand receptors.
REACT_353304. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucagon receptor
Short name:
GL-R
Gene namesi
Name:Gcgr
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi2669. Gcgr.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini27 – 137111ExtracellularBy similarityAdd
BLAST
Transmembranei138 – 16225Helical; Name=1By similarityAdd
BLAST
Topological domaini163 – 17412CytoplasmicBy similarityAdd
BLAST
Transmembranei175 – 19925Helical; Name=2By similarityAdd
BLAST
Topological domaini200 – 22627ExtracellularBy similarityAdd
BLAST
Transmembranei227 – 25024Helical; Name=3By similarityAdd
BLAST
Topological domaini251 – 26414CytoplasmicBy similarityAdd
BLAST
Transmembranei265 – 28622Helical; Name=4By similarityAdd
BLAST
Topological domaini287 – 30418ExtracellularBy similarityAdd
BLAST
Transmembranei305 – 32723Helical; Name=5By similarityAdd
BLAST
Topological domaini328 – 35124CytoplasmicBy similarityAdd
BLAST
Transmembranei352 – 37019Helical; Name=6By similarityAdd
BLAST
Topological domaini371 – 38212ExtracellularBy similarityAdd
BLAST
Transmembranei383 – 40321Helical; Name=7By similarityAdd
BLAST
Topological domaini404 – 48582CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • endosome Source: RGD
  • integral component of membrane Source: RGD
  • integral component of plasma membrane Source: Ensembl
  • plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence AnalysisAdd
BLAST
Chaini27 – 485459Glucagon receptorPRO_0000012834Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi44 ↔ 68By similarity
Glycosylationi47 – 471N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi59 ↔ 101By similarity
Glycosylationi60 – 601N-linked (GlcNAc...)Sequence Analysis
Glycosylationi75 – 751N-linked (GlcNAc...)Sequence Analysis
Glycosylationi79 – 791N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi82 ↔ 122By similarity
Disulfide bondi225 ↔ 295By similarity
Modified residuei460 – 4601PhosphoserineBy similarity

Post-translational modificationi

Ligand-binding promotes phosphorylation of serine residues in the C-terminal cytoplasmic domain. Phosphorylation is important for receptor endocytosis after ligand-binding (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP30082.
PRIDEiP30082.

PTM databases

PhosphoSiteiP30082.

Expressioni

Gene expression databases

GenevisibleiP30082. RN.

Interactioni

Protein-protein interaction databases

MINTiMINT-4996622.
STRINGi10116.ENSRNOP00000051845.

Structurei

3D structure databases

ProteinModelPortaliP30082.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG263329.
GeneTreeiENSGT00760000118800.
HOGENOMiHOG000008250.
HOVERGENiHBG008318.
InParanoidiP30082.
KOiK04583.
OMAiCQMDGEE.
OrthoDBiEOG7TF78W.
PhylomeDBiP30082.
TreeFamiTF315710.

Family and domain databases

InterProiIPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR003290. GPCR_2_GLP1/glucagon_rcpt.
IPR003291. GPCR_2_glucagon_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF02793. HRM. 1 hit.
[Graphical view]
PRINTSiPR01353. GLUCAGNFAMLY.
PR01354. GLUCAGONR.
PR00249. GPCRSECRETIN.
SMARTiSM00008. HormR. 1 hit.
[Graphical view]
PROSITEiPS00649. G_PROTEIN_RECEP_F2_1. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30082-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLTQLHCPY LLLLLVVLSC LPKAPSAQVM DFLFEKWKLY SDQCHHNLSL
60 70 80 90 100
LPPPTELVCN RTFDKYSCWP DTPPNTTANI SCPWYLPWYH KVQHRLVFKR
110 120 130 140 150
CGPDGQWVRG PRGQSWRDAS QCQMDDDEIE VQKGVAKMYS SYQVMYTVGY
160 170 180 190 200
SLSLGALLLA LVILLGLRKL HCTRNYIHGN LFASFVLKAG SVLVIDWLLK
210 220 230 240 250
TRYSQKIGDD LSVSVWLSDG AVAGCRVATV IMQYGIIANY CWLLVEGVYL
260 270 280 290 300
YSLLSITTFS EKSFFSLYLC IGWGSPLLFV IPWVVVKCLF ENVQCWTSND
310 320 330 340 350
NMGFWWILRI PVLLAILINF FIFVRIIHLL VAKLRAHQMH YADYKFRLAR
360 370 380 390 400
STLTLIPLLG VHEVVFAFVT DEHAQGTLRS TKLFFDLFFS SFQGLLVAVL
410 420 430 440 450
YCFLNKEVQA ELLRRWRRWQ EGKALQEERM ASSHGSHMAP AGTCHGDPCE
460 470 480
KLQLMSAGSS SGTGCEPSAK TSLASSLPRL ADSPT
Length:485
Mass (Da):55,038
Last modified:February 1, 1996 - v2
Checksum:i91AC67D7A4F5090E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti216 – 2161W → C (PubMed:8384842).Curated
Sequence conflicti324 – 3241V → A (PubMed:8384842).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L04796 mRNA. Translation: AAA16439.1.
X68692 mRNA. Translation: CAA48651.1.
M96674 mRNA. Translation: AAA02992.1.
U63021 Genomic DNA. Translation: AAB16800.1.
PIRiJQ1957.
RefSeqiNP_742088.1. NM_172091.2.
NP_742089.1. NM_172092.2.
XP_006247938.1. XM_006247876.2.
UniGeneiRn.11225.

Genome annotation databases

EnsembliENSRNOT00000054962; ENSRNOP00000051845; ENSRNOG00000036692.
ENSRNOT00000083601; ENSRNOP00000074929; ENSRNOG00000036692.
GeneIDi24953.
KEGGirno:24953.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L04796 mRNA. Translation: AAA16439.1.
X68692 mRNA. Translation: CAA48651.1.
M96674 mRNA. Translation: AAA02992.1.
U63021 Genomic DNA. Translation: AAB16800.1.
PIRiJQ1957.
RefSeqiNP_742088.1. NM_172091.2.
NP_742089.1. NM_172092.2.
XP_006247938.1. XM_006247876.2.
UniGeneiRn.11225.

3D structure databases

ProteinModelPortaliP30082.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4996622.
STRINGi10116.ENSRNOP00000051845.

Chemistry

BindingDBiP30082.
ChEMBLiCHEMBL4720.
GuidetoPHARMACOLOGYi251.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP30082.

Proteomic databases

PaxDbiP30082.
PRIDEiP30082.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000054962; ENSRNOP00000051845; ENSRNOG00000036692.
ENSRNOT00000083601; ENSRNOP00000074929; ENSRNOG00000036692.
GeneIDi24953.
KEGGirno:24953.

Organism-specific databases

CTDi2642.
RGDi2669. Gcgr.

Phylogenomic databases

eggNOGiNOG263329.
GeneTreeiENSGT00760000118800.
HOGENOMiHOG000008250.
HOVERGENiHBG008318.
InParanoidiP30082.
KOiK04583.
OMAiCQMDGEE.
OrthoDBiEOG7TF78W.
PhylomeDBiP30082.
TreeFamiTF315710.

Enzyme and pathway databases

ReactomeiREACT_287445. G alpha (s) signalling events.
REACT_311068. Glucagon signaling in metabolic regulation.
REACT_326473. Glucagon-type ligand receptors.
REACT_353304. G alpha (q) signalling events.

Miscellaneous databases

NextBioi604975.
PROiP30082.

Gene expression databases

GenevisibleiP30082. RN.

Family and domain databases

InterProiIPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR003290. GPCR_2_GLP1/glucagon_rcpt.
IPR003291. GPCR_2_glucagon_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF02793. HRM. 1 hit.
[Graphical view]
PRINTSiPR01353. GLUCAGNFAMLY.
PR01354. GLUCAGONR.
PR00249. GPCRSECRETIN.
SMARTiSM00008. HormR. 1 hit.
[Graphical view]
PROSITEiPS00649. G_PROTEIN_RECEP_F2_1. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
  2. "Small introns in a hepatic cDNA encoding a new glucagon-like peptide 1-type receptor."
    Svoboda M., Ciccarelli E., Tastenoy M., Cauvin A., Stievenart M., Christophe J.
    Biochem. Biophys. Res. Commun. 191:479-486(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Sequencing of eleven introns in genomic DNA encoding rat glucagon receptor and multiple alternative splicing of its mRNA."
    Maget B., Tastenoy M., Svoboda M.
    FEBS Lett. 351:271-275(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Wistar.
    Tissue: Liver.

Entry informationi

Entry nameiGLR_RAT
AccessioniPrimary (citable) accession number: P30082
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: February 1, 1996
Last modified: July 22, 2015
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.