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P30082

- GLR_RAT

UniProt

P30082 - GLR_RAT

Protein

Glucagon receptor

Gene

Gcgr

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    G-protein coupled receptor for glucagon that plays a central role in the regulation of blood glucose levels and glucose homeostasis. Regulates the rate of hepatic glucose production by promoting glycogen hydrolysis and gluconeogenesis. Plays an important role in mediating the responses to fasting. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Promotes activation of adenylate cyclase. Besides, plays a role in signaling via a phosphatidylinositol-calcium second messenger system.1 Publication

    GO - Molecular functioni

    1. glucagon receptor activity Source: RGD
    2. peptide hormone binding Source: RGD

    GO - Biological processi

    1. adenylate cyclase-activating G-protein coupled receptor signaling pathway Source: RGD
    2. adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: RGD
    3. exocytosis Source: RGD
    4. glucose homeostasis Source: UniProtKB
    5. G-protein coupled receptor signaling pathway Source: RGD
    6. hormone-mediated signaling pathway Source: RGD
    7. regulation of glycogen metabolic process Source: UniProtKB
    8. response to starvation Source: UniProtKB

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucagon receptor
    Short name:
    GL-R
    Gene namesi
    Name:Gcgr
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 10

    Organism-specific databases

    RGDi2669. Gcgr.

    Subcellular locationi

    Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
    Note: Is rapidly internalized after ligand-binding.By similarity

    GO - Cellular componenti

    1. endosome Source: RGD
    2. integral component of membrane Source: RGD
    3. integral component of plasma membrane Source: Ensembl
    4. plasma membrane Source: RGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2626Sequence AnalysisAdd
    BLAST
    Chaini27 – 485459Glucagon receptorPRO_0000012834Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi44 ↔ 68By similarity
    Glycosylationi47 – 471N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi59 ↔ 101By similarity
    Glycosylationi60 – 601N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi75 – 751N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi79 – 791N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi82 ↔ 122By similarity

    Post-translational modificationi

    Ligand-binding promotes phosphorylation of serine residues in the C-terminal cytoplasmic domain. Phosphorylation is important for receptor endocytosis after ligand-binding By similarity.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiP30082.
    PRIDEiP30082.

    PTM databases

    PhosphoSiteiP30082.

    Expressioni

    Gene expression databases

    GenevestigatoriP30082.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-4996622.
    STRINGi10116.ENSRNOP00000051845.

    Structurei

    3D structure databases

    ProteinModelPortaliP30082.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini27 – 137111ExtracellularBy similarityAdd
    BLAST
    Topological domaini163 – 17412CytoplasmicBy similarityAdd
    BLAST
    Topological domaini200 – 22627ExtracellularBy similarityAdd
    BLAST
    Topological domaini251 – 26414CytoplasmicBy similarityAdd
    BLAST
    Topological domaini287 – 30418ExtracellularBy similarityAdd
    BLAST
    Topological domaini328 – 35124CytoplasmicBy similarityAdd
    BLAST
    Topological domaini371 – 38212ExtracellularBy similarityAdd
    BLAST
    Topological domaini404 – 48582CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei138 – 16225Helical; Name=1By similarityAdd
    BLAST
    Transmembranei175 – 19925Helical; Name=2By similarityAdd
    BLAST
    Transmembranei227 – 25024Helical; Name=3By similarityAdd
    BLAST
    Transmembranei265 – 28622Helical; Name=4By similarityAdd
    BLAST
    Transmembranei305 – 32723Helical; Name=5By similarityAdd
    BLAST
    Transmembranei352 – 37019Helical; Name=6By similarityAdd
    BLAST
    Transmembranei383 – 40321Helical; Name=7By similarityAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG263329.
    GeneTreeiENSGT00740000115231.
    HOGENOMiHOG000008250.
    HOVERGENiHBG008318.
    InParanoidiP30082.
    KOiK04583.
    OMAiLPWHHKV.
    OrthoDBiEOG7TF78W.
    PhylomeDBiP30082.
    TreeFamiTF315710.

    Family and domain databases

    InterProiIPR017981. GPCR_2-like.
    IPR001879. GPCR_2_extracellular_dom.
    IPR003290. GPCR_2_GLP1/glucagon_rcpt.
    IPR003291. GPCR_2_glucagon_rcpt.
    IPR000832. GPCR_2_secretin-like.
    IPR017983. GPCR_2_secretin-like_CS.
    [Graphical view]
    PfamiPF00002. 7tm_2. 1 hit.
    PF02793. HRM. 1 hit.
    [Graphical view]
    PRINTSiPR01353. GLUCAGNFAMLY.
    PR01354. GLUCAGONR.
    PR00249. GPCRSECRETIN.
    SMARTiSM00008. HormR. 1 hit.
    [Graphical view]
    PROSITEiPS00649. G_PROTEIN_RECEP_F2_1. 1 hit.
    PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
    PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
    PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P30082-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLLTQLHCPY LLLLLVVLSC LPKAPSAQVM DFLFEKWKLY SDQCHHNLSL    50
    LPPPTELVCN RTFDKYSCWP DTPPNTTANI SCPWYLPWYH KVQHRLVFKR 100
    CGPDGQWVRG PRGQSWRDAS QCQMDDDEIE VQKGVAKMYS SYQVMYTVGY 150
    SLSLGALLLA LVILLGLRKL HCTRNYIHGN LFASFVLKAG SVLVIDWLLK 200
    TRYSQKIGDD LSVSVWLSDG AVAGCRVATV IMQYGIIANY CWLLVEGVYL 250
    YSLLSITTFS EKSFFSLYLC IGWGSPLLFV IPWVVVKCLF ENVQCWTSND 300
    NMGFWWILRI PVLLAILINF FIFVRIIHLL VAKLRAHQMH YADYKFRLAR 350
    STLTLIPLLG VHEVVFAFVT DEHAQGTLRS TKLFFDLFFS SFQGLLVAVL 400
    YCFLNKEVQA ELLRRWRRWQ EGKALQEERM ASSHGSHMAP AGTCHGDPCE 450
    KLQLMSAGSS SGTGCEPSAK TSLASSLPRL ADSPT 485
    Length:485
    Mass (Da):55,038
    Last modified:February 1, 1996 - v2
    Checksum:i91AC67D7A4F5090E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti216 – 2161W → C(PubMed:8384842)Curated
    Sequence conflicti324 – 3241V → A(PubMed:8384842)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L04796 mRNA. Translation: AAA16439.1.
    X68692 mRNA. Translation: CAA48651.1.
    M96674 mRNA. Translation: AAA02992.1.
    U63021 Genomic DNA. Translation: AAB16800.1.
    PIRiJQ1957.
    RefSeqiNP_742088.1. NM_172091.2.
    NP_742089.1. NM_172092.2.
    XP_006247938.1. XM_006247876.1.
    UniGeneiRn.11225.

    Genome annotation databases

    EnsembliENSRNOT00000054962; ENSRNOP00000051845; ENSRNOG00000036692.
    GeneIDi24953.
    KEGGirno:24953.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L04796 mRNA. Translation: AAA16439.1 .
    X68692 mRNA. Translation: CAA48651.1 .
    M96674 mRNA. Translation: AAA02992.1 .
    U63021 Genomic DNA. Translation: AAB16800.1 .
    PIRi JQ1957.
    RefSeqi NP_742088.1. NM_172091.2.
    NP_742089.1. NM_172092.2.
    XP_006247938.1. XM_006247876.1.
    UniGenei Rn.11225.

    3D structure databases

    ProteinModelPortali P30082.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4996622.
    STRINGi 10116.ENSRNOP00000051845.

    Chemistry

    BindingDBi P30082.
    ChEMBLi CHEMBL4720.
    GuidetoPHARMACOLOGYi 251.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P30082.

    Proteomic databases

    PaxDbi P30082.
    PRIDEi P30082.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000054962 ; ENSRNOP00000051845 ; ENSRNOG00000036692 .
    GeneIDi 24953.
    KEGGi rno:24953.

    Organism-specific databases

    CTDi 2642.
    RGDi 2669. Gcgr.

    Phylogenomic databases

    eggNOGi NOG263329.
    GeneTreei ENSGT00740000115231.
    HOGENOMi HOG000008250.
    HOVERGENi HBG008318.
    InParanoidi P30082.
    KOi K04583.
    OMAi LPWHHKV.
    OrthoDBi EOG7TF78W.
    PhylomeDBi P30082.
    TreeFami TF315710.

    Miscellaneous databases

    NextBioi 604975.
    PROi P30082.

    Gene expression databases

    Genevestigatori P30082.

    Family and domain databases

    InterProi IPR017981. GPCR_2-like.
    IPR001879. GPCR_2_extracellular_dom.
    IPR003290. GPCR_2_GLP1/glucagon_rcpt.
    IPR003291. GPCR_2_glucagon_rcpt.
    IPR000832. GPCR_2_secretin-like.
    IPR017983. GPCR_2_secretin-like_CS.
    [Graphical view ]
    Pfami PF00002. 7tm_2. 1 hit.
    PF02793. HRM. 1 hit.
    [Graphical view ]
    PRINTSi PR01353. GLUCAGNFAMLY.
    PR01354. GLUCAGONR.
    PR00249. GPCRSECRETIN.
    SMARTi SM00008. HormR. 1 hit.
    [Graphical view ]
    PROSITEi PS00649. G_PROTEIN_RECEP_F2_1. 1 hit.
    PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
    PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
    PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
    2. "Small introns in a hepatic cDNA encoding a new glucagon-like peptide 1-type receptor."
      Svoboda M., Ciccarelli E., Tastenoy M., Cauvin A., Stievenart M., Christophe J.
      Biochem. Biophys. Res. Commun. 191:479-486(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Sequencing of eleven introns in genomic DNA encoding rat glucagon receptor and multiple alternative splicing of its mRNA."
      Maget B., Tastenoy M., Svoboda M.
      FEBS Lett. 351:271-275(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Wistar.
      Tissue: Liver.

    Entry informationi

    Entry nameiGLR_RAT
    AccessioniPrimary (citable) accession number: P30082
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 110 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3