P30079 (CHSY_PINSY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chalcone synthase
EC=2.3.1.74

Alternative name(s):
Naringenin-chalcone synthase

Gene names

Name:

CHS

Organism

Pinus sylvestris (Scots pine)

Taxonomic identifier

3349 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Coniferophyta › Coniferopsida › Coniferales › Pinaceae › Pinus › Pinus

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.
Catalytic activity	3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.
Pathway	Secondary metabolite biosynthesis; flavonoid biosynthesis.
Sequence similarities	Belongs to the chalcone/stilbene synthases family.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological_process	flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	naringenin-chalcone synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 396	396	Chalcone synthase		PRO_0000216042
Sites
	Active site	169	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P30079 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 5B5CFAEE888173B4	FASTA	396	43,294
	10 20 30 40 50 60 MAAGMMKDLE AFRKAQRADG PATILAIGTA TPPNAVDQSS YPDYYFKITN SEHMTELKEK 70 80 90 100 110 120 FRRMCDKSAI KKRYMYLTEE ILKENPKVCE YMAPSLDARQ DMVVVEVPRL GKEAAAKAIK 130 140 150 160 170 180 EWGQPKSKIT HVIFCTTSGV DMPGADYQLT KLLGLRPSVK RVMMYQQGCF AGGTVLRVAK 190 200 210 220 230 240 DLAENNRGAR VLVVCSEITA VTFRGPSDTH LDSMVGQALF GDGAAALIVG ADPVPEVEKP 250 260 270 280 290 300 CFELMWTAQT ILPDSDGAID GHLREVGLTF HLLKDVPGLI SKNIEKSLVE AFQQFGISDW 310 320 330 340 350 360 NQLFWIAHPG GPAILDQVEA KLNLDPKKLS ATRQVLSDYG NMSSACVHFI LDEMRKSSKE 370 380 390 KGCSTTGEGL DVGVLFGFGP GLTVETVVLK SVPLLD

References

[1]

"Molecular analysis of chalcone and dihydropinosylvin synthase from Scots pine (Pinus sylvestris), and differential regulation of these and related enzyme activities in stressed plants." Fliegmann J., Schroeder G., Schanz S., Britsch L., Schroeder J. Plant Mol. Biol. 18:489-503(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X60754 Genomic DNA. Translation: CAA43166.1.
PIR	S20515.
3D structure databases
ProteinModelPortal	P30079.
SMR	P30079. Positions 13-394.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-11832.
UniPathway	UPA00154.
Family and domain databases
Gene3D	3.40.47.10. 2 hits.
InterPro	IPR012328. Chalcone/stilbene_synth_C. IPR018088. Chalcone/stilbene_synthase_AS. IPR001099. Chalcone/stilbene_synthase_N. IPR011141. Polyketide_synthase_type-III. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Pfam	PF02797. Chal_sti_synt_C. 1 hit. PF00195. Chal_sti_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000451. PKS_III. 1 hit.
SUPFAM	SSF53901. Thiolase-like. 2 hits.
PROSITE	PS00441. CHALCONE_SYNTH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHSY_PINSY

Accession

Primary (citable) accession number: P30079

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	April 3, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program