Reviewed, UniProtKB/Swiss-Prot P30074 (CHS2_MEDSA)

Names and origin

Protein names

Recommended name:
    Chalcone synthase 2
    EC=2.3.1.74
Alternative name(s):
    Naringenin-chalcone synthase 2

Gene names

Name:

CHS2

Organism

Medicago sativa (Alfalfa)

Taxonomic identifier

3879 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Trifolieae › Medicago

Protein attributes

Sequence length	389 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.
Catalytic activity	3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.
Pathway	Secondary metabolite biosynthesis; flavonoid biosynthesis.
Sequence similarities	Belongs to the chalcone/stilbene synthases family.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Molecular function	Acyltransferase Transferase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	acyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW naringenin-chalcone synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 389

389

Chalcone synthase 2

PRO_0000216007

Sites

Active site

164

1

Secondary structure

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389

Helix Strand Turn

Details...

Helix

4 – 11

8

Beta strand

18 – 25

8

Beta strand

30 – 32

3

Helix

33 – 35

3

Helix

36 – 43

8

Helix

50 – 62

13

Beta strand

67 – 69

3

Helix

74 – 79

6

Helix

81 – 84

4

Beta strand

85 – 87

3

Helix

91 – 117

27

Helix

121 – 123

3

Beta strand

126 – 133

8

Helix

140 – 148

9

Beta strand

155 – 161

7

Helix

166 – 179

14

Beta strand

185 – 192

8

Helix

194 – 196

3

Helix

206 – 214

9

Beta strand

218 – 227

10

Turn

230 – 232

3

Beta strand

237 – 246

10

Beta strand

253 – 259

7

Beta strand

262 – 267

6

Helix

271 – 287

17

Helix

288 – 290

3

Beta strand

297 – 302

6

Helix

307 – 317

11

Helix

321 – 324

4

Helix

325 – 334

10

Helix

338 – 340

3

Helix

341 – 355

15

Turn

361 – 364

4

Beta strand

366 – 374

9

Turn

375 – 377

3

Beta strand

378 – 386

9

Sequences

	Sequence		Length	Mass (Da)
	P30074-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: E03422EE332A5408	FASTA	389	42,706
	10 20 30 40 50 60 MVSVSEIRKA QRAEGPATIL AIGTANPANC VEQSTYPDFY FKITNSEHKT ELKEKFQRMC 70 80 90 100 110 120 DKSMIKRRYM YLTEEILKEN PNVCEYMAPS LDARQDMVVV EVPRLGKEAA VKAIKEWGQP 130 140 150 160 170 180 KSKITHLIVC TTSGVDMPGA DYQLTKLLGL RPYVKRYMMY QQGCFAGGTV LRLAKDLAEN 190 200 210 220 230 240 NKGARVLVVC SEVTAVTFRG PSDTHLDSLV GQALFGDGAA ALIVGSDPVP EIEKPIFEMV 250 260 270 280 290 300 WTAQTIAPDS EGAIDGHLRE AGLTFHLLKD VPGIVSKNIT KALVEAFEPL GISDYNSIFW 310 320 330 340 350 360 IAHPGGPAIL DQVEQKLALK PEKMNATREV LSEYGNMSSA CVLFILDEMR KKSTQNGLKT 370 380 TGEGLEWGVL FGFGPGLTIE TVVLRSVAI

References

[1]	"Stress responses in alfalfa (Medicago sativa L.). 15. Characterization and expression patterns of members of a subset of the chalcone synthase multigene family." Junghans H., Dalkin K., Dixon R.A. Plant Mol. Biol. 22:239-253(1993) [PubMed: 8507827] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Dissection of malonyl-coenzyme A decarboxylation from polyketide formation in the reaction mechanism of a plant polyketide synthase." Jez J.M., Ferrer J.L., Bowman M.E., Dixon R.A., Noel J.P. Biochemistry 39:890-902(2000) [PubMed: 10653632] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.69 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L02902 mRNA. Translation: AAA02824.1.

PIR

S35164.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BI5	X-ray	1.56	A	1-389	[»]
1BQ6	X-ray	1.56	A	2-389	[»]
1CGK	X-ray	1.84	A	1-389	[»]
1CGZ	X-ray	1.70	A	1-389	[»]
1CHW	X-ray	1.90	A/B	1-389	[»]
1CML	X-ray	1.69	A	1-389	[»]
1D6F	X-ray	1.69	A	1-389	[»]
1D6H	X-ray	2.15	A	3-389	[»]
1D6I	X-ray	2.00	A/B	2-389	[»]
1I86	X-ray	1.50	A	1-389	[»]
1I88	X-ray	1.45	A/B	1-389	[»]
1I89	X-ray	1.86	A/B	1-389	[»]
1I8B	X-ray	1.95	A/B	1-389	[»]
1JWX	X-ray	1.63	A	1-389	[»]
1U0V	X-ray	1.90	A/B	1-389	[»]
1U0W	X-ray	2.00	A/B/C/D	1-389	[»]

ModBase

Search...

Enzyme and pathway databases

BRENDA

2.3.1.74. 815.

Family and domain databases

InterPro

IPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]

Gene3D

G3DSA:3.40.47.10. Thiolase-like_subgr. 2 hits.

Pfam

PF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]

PIRSF

PIRSF000451. PKS_III. 1 hit.

PROSITE

PS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

CHS2_MEDSA

Accession

Primary (citable) accession number: P30074

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	February 9, 2010
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)