P30052 (SCAL_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein scalloped

Gene names

Name:	sd
ORF Names:	CG8544

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcription factor which plays a key role in the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein Hippo (Hpo), in complex with its regulatory protein Salvador (Sav), phosphorylates and activates Warts (Wts) in complex with its regulatory protein Mats, which in turn phosphorylates and inactivates the Yorkie (Yki) oncoprotein. The Hippo/SWH signaling pathway inhibits the activity of the transcriptional complex formed by Scalloped (sd) and Yki and the target genes of this pathway include cyclin-E (cycE), diap1 and bantam. Sd promotes nuclear localization of Yki. Involved in the regulation of cell-specific gene expression during development, particularly in the differentiation of the nervous system. When in combination with vestigial (vg) it acts as a transcriptional activation complex that regulates gene expression in the wing. Binding to vg switches the DNA target selectivity of sd. Required autonomously for cell proliferation and viability within the wing blade. Required for proper sensory organ precursor (SOP) differentiation at the wing margin; required for correct expression of sens. Ref.4 Ref.5 Ref.6 Ref.8 Ref.9 Ref.10
Subunit structure	The C-terminus of sd interacts with the C-terminal serine-rich protein domain of vg, to form a complex which acts as a selector for wing development. Interacts (via C-terminus) with yki (via N-terminus) and this interaction enhances its transcriptional activity. Ref.4 Ref.5 Ref.8 Ref.9
Subcellular location	Nucleus.
Tissue specificity	Subset of neuroblasts in the central nervous system and in the peripheral sense organs of the embryo. Expressed in the developing wing primordia initially along the D/V wing boundary, and by the late third larval instar, maximal expression is seen in cells at the D/V wing disk boundary. Less expression in cells located farther from this boundary. Also expressed in wing progenitor cells. Ref.6
Sequence similarities	Contains 1 TEA DNA-binding domain.

Ontologies

Keywords
Biological process	Cell cycle Differentiation Transcription Transcription regulation
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cardiac muscle cell development Inferred from mutant phenotype PubMed 18987343. Source: FlyBase cell cycle Inferred from electronic annotation. Source: UniProtKB-KW compound eye morphogenesis Inferred from mutant phenotype PubMed 17110491. Source: FlyBase hippo signaling cascade Inferred from electronic annotation. Source: InterPro imaginal disc-derived leg morphogenesis Inferred from mutant phenotype PubMed 17110491. Source: FlyBase imaginal disc-derived wing morphogenesis Inferred from direct assay Ref.4. Source: UniProtKB positive regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype Ref.5. Source: FlyBase somatic muscle development Inferred from mutant phenotype PubMed 18987343. Source: FlyBase stem cell proliferation Inferred from direct assay PubMed 21078993. Source: FlyBase transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from direct assay Ref.4. Source: UniProtKB
Molecular_function	RNA polymerase II distal enhancer sequence-specific DNA binding Inferred from direct assay Ref.5. Source: FlyBase metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
vg	Q26366	7	EBI-151228,EBI-162687

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 440	440	Protein scalloped		PRO_0000205942
Regions
	DNA binding	88 – 155	68	TEA Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P30052 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: B885D7F713473A79	FASTA	440	49,658
	10 20 30 40 50 60 MKNITSSSTC STGLLQLQNN LSCSELEVAE KTEQQAVGPG TIPSPWTPVN AGPPGALGSA 70 80 90 100 110 120 DTNGSMVDSK NLDVGDMSDD EKDLSSADAE GVWSPDIEQS FQEALSIYPP CGRRKIILSD 130 140 150 160 170 180 EGKMYGRNEL IARYIKLRTG KTRTRKQVSS HIQVLARRKL REIQAKIKVQ FWQPGLQPST 190 200 210 220 230 240 SQDFYDYSIK PFPQPPYPAG KTSTAVSGDE TGIPPSQLPW EGRAIATHKF RLLEFTAFME 250 260 270 280 290 300 IQRDEIYHRH LFVQLGGKPS FSDPLLETVD IRQIFDKFPE KSGGLKDLYE KGPQNAFYLV 310 320 330 340 350 360 KCWADLNTDL TTGSETGDFY GVTSQYESNE NVVLVCSTIV CSFGKQVVEK VESEYSRLEN 370 380 390 400 410 420 NRYVYRIQRS PMCEYMINFI QKLKNLPERY MMNSVLENFT ILQVMRARET QETLLCIAYV 430 440 FEVAAQNSGT THHIYRLIKE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The scalloped gene encodes a novel, evolutionarily conserved transcription factor required for sensory organ differentiation in Drosophila." Campbell S.D., Inamdar M., Rodrigues V., Raghavan V., Palazzolo M., Chovnick A. Genes Dev. 6:367-379(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"Molecular interactions between Vestigial and Scalloped promote wing formation in Drosophila." Simmonds A.J., Liu X., Soanes K.H., Krause H.M., Irvine K.D., Bell J.B. Genes Dev. 12:3815-3820(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH VG.
[5]	"The Vestigial and Scalloped proteins act together to directly regulate wing-specific gene expression in Drosophila." Halder G., Polaczyk P., Kraus M.E., Hudson A., Kim J., Laughon A., Carroll S.B. Genes Dev. 12:3900-3909(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH VG.
[6]	"Roles for scalloped and vestigial in regulating cell affinity and interactions between the wing blade and the wing hinge." Liu X., Grammont M., Irvine K.D. Dev. Biol. 228:287-303(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[7]	"Binding of the vestigial co-factor switches the DNA-target selectivity of the scalloped selector protein." Halder G., Carroll S.B. Development 128:3295-3305(2001) [PubMed] [Europe PMC] [Abstract] Cited for: DNA-BINDING OF VG-SD COMPLEX.
[8]	"Further developmental roles of the vestigial/scalloped transcription complex during wing development in Drosophila melanogaster." Srivastava A., Bell J.B. Mech. Dev. 120:587-596(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH VG.
[9]	"SCALLOPED interacts with YORKIE, the nuclear effector of the hippo tumor-suppressor pathway in Drosophila." Goulev Y., Fauny J.D., Gonzalez-Marti B., Flagiello D., Silber J., Zider A. Curr. Biol. 18:435-441(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH YKI.
[10]	"The TEAD/TEF family of transcription factor Scalloped mediates Hippo signaling in organ size control." Zhang L., Ren F., Zhang Q., Chen Y., Wang B., Jiang J. Dev. Cell 14:377-387(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M83787 mRNA. Translation: AAA28881.1. AE014298 Genomic DNA. Translation: AAF48521.1.
PIR	A42136.
RefSeq	NP_001096990.1. NM_001103520.2. NP_001096991.1. NM_001103521.2. NP_001096992.1. NM_001103522.2. NP_001245697.1. NM_001258768.1. NP_001259582.1. NM_001272653.1. NP_001259583.1. NM_001272654.1. NP_511169.1. NM_078614.4.
UniGene	Dm.6072.
3D structure databases
ProteinModelPortal	P30052.
SMR	P30052. Positions 88-163, 223-439.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-21510N.
IntAct	P30052. 3 interactions.
MINT	MINT-809557.
Proteomic databases
PaxDb	P30052.
PRIDE	P30052.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0074099; FBpp0073914; FBgn0003345. FBtr0112825; FBpp0111737; FBgn0003345. FBtr0112826; FBpp0111738; FBgn0003345. FBtr0112827; FBpp0111739; FBgn0003345. FBtr0308129; FBpp0300451; FBgn0003345. FBtr0310312; FBpp0301995; FBgn0003345. FBtr0310313; FBpp0301996; FBgn0003345.
GeneID	32536.
KEGG	dme:Dmel_CG8544.
Organism-specific databases
CTD	109765.
FlyBase	FBgn0003345. sd.
Phylogenomic databases
eggNOG	NOG246992.
GeneTree	ENSGT00390000008670.
InParanoid	P30052.
KO	K09448.
OMA	ERKDVFF.
OrthoDB	EOG47H45B.
PhylomeDB	P30052.
Gene expression databases
Bgee	P30052.
GermOnline	CG8544. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000818. TEA/ATTS. IPR016361. TEF. [Graphical view]
PANTHER	PTHR11834. PTHR11834. 1 hit.
Pfam	PF01285. TEA. 2 hits. [Graphical view]
PIRSF	PIRSF002603. TEF. 1 hit.
PRINTS	PR00065. TEADOMAIN.
SMART	SM00426. TEA. 1 hit. [Graphical view]
PROSITE	PS00554. TEA_1. 1 hit. PS51088. TEA_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SD. drosophila.
GenomeRNAi	32536.
NextBio	778983.

Entry information

Entry name

SCAL_DROME

Accession

Primary (citable) accession number: P30052
Secondary accession number(s): Q9VXN7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	May 1, 2013
This is version 116 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project